LOCUS       AiP15245                4978 bp    DNA     circular UNA 14-OCT-2024
DEFINITION  pAAV-AiE1058q_3xC2-minBG-SYFP2-WPRE3-BGHpA.
ACCESSION   urn.local.aaron.oster.6u2-it6u6ma
VERSION     urn.local.aaron.oster.6u2-it6u6ma
KEYWORDS    .
SOURCE       (pAAV-AiE1058rh_3xC2-minBG-SYFP2-WPRE3-BGHpA)
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    complement(1..141)
                     /note="Geneious type: LTR"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ITR"
     misc_feature    156..902
                     /note="Geneious type: Bashed enhancer"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AiE1058q_3xC2"
     regulatory      909..961
                     /note="Geneious type: promoter"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="minBG promoter"
     regulatory      996..1005
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Kozak sequence"
     CDS             1002..1721
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SYFP2"
     3'UTR           1756..2001
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="WPRE3"
     misc_feature    2015..2218
                     /note="Geneious type: polyA_signal"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="BGH-pA"
     misc_feature    2241..2381
                     /note="Geneious type: LTR"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ITR"
     rep_origin      2473..2779
     regulatory      3193..3297
                     /note="Geneious type: promoter"
                     /gene="bla"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR promoter"
     CDS             3298..4158
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR"
     rep_origin      4306..4973
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaatca gttttacacg gagtaaagca
      181 aacagttatg tcagctaatt gcacttaatt acagagacaa ttgctgtttc aaaatgttgg
      241 tgtgtccact tcttcatctt tcaatcagtt caagaacatc cagccccaac tgtgaacatt
      301 ttaattctgc aattttaatt tcacttctgg aaaggaactt cagaaaaaga ctcagcaagg
      361 atctccatta acaagacagc actccctggg tacccagagg tggcaatcag ttttacacgg
      421 agtaaagcaa acagttatgt cagctaattg cacttaatta cagagacaat tgctgtttca
      481 aaatgttggt gtgtccactt cttcatcttt caatcagttc aagaacatcc agccccaact
      541 gtgaacattt taattctgca attttaattt cacttctgga aaggaacttc agaaaaagac
      601 tcagcaagga tctccattaa caagacagca ctccctgggt acccagaggt ggcaatcagt
      661 tttacacgga gtaaagcaaa cagttatgtc agctaattgc acttaattac agagacaatt
      721 gctgtttcaa aatgttggtg tgtccacttc ttcatctttc aatcagttca agaacatcca
      781 gccccaactg tgaacatttt aattctgcaa ttttaatttc acttctggaa aggaacttca
      841 gaaaaagact cagcaaggat ctccattaac aagacagcac tccctgggta cccagaggtg
      901 gcgagctcgg gctgggcata aaagtcaggg cagagccatc tattgcttac atttgcttct
      961 gggatccaga tctttcgaag ctagcgctac cggtcgccac catggtgagc aagggcgagg
     1021 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1081 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc
     1141 tgatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgggct
     1201 acggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1261 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1321 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1381 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     1441 acagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca
     1501 agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca
     1561 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtcca
     1621 agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     1681 ccgccgggat cactctcggc atggacgagc tgtacaagta agtcgacggc gcgccgcggc
     1741 cgcgaattcg atatcataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     1801 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     1861 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttagttc
     1921 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     1981 tgggcactga caattccgtg gctcgagaga tcttcgactg tgccttctag ttgccagcca
     2041 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     2101 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     2161 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgca
     2221 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
     2281 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     2341 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
     2401 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     2461 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     2521 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     2581 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     2641 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     2701 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     2761 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg
     2821 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     2881 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     2941 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     3001 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     3061 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     3121 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     3181 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     3241 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     3301 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     3361 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     3421 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     3481 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     3541 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     3601 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     3661 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     3721 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     3781 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     3841 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3901 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3961 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     4021 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     4081 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     4141 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     4201 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     4261 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     4321 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     4381 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     4441 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     4501 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4561 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4621 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4681 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4741 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4801 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4861 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4921 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
