LOCUS       AiP15708_pAAV-AiE1129m_3xC1-minBG-SYFP2-WPRE3-BGHpA        5041 bp    DNA     linear   UNA 03-JAN-2025
DEFINITION  .
ACCESSION   urn.local...eu-ho79f3j
VERSION     urn.local...eu-ho79f3j
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="L-ITR"
     misc_feature    156..970
                     /Original_Bases="ATAGGTACCGAGCTCTATGCACTCACAGTGGTTTGGCATGC
                     ATCTGGTGAATTTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTC
                     TCCCTAACGTAATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGC
                     CTCTTCCATTCTTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGT
                     CTCTGCAATTTGTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTG
                     TTCCTGAATCTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGT
                     AATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAA
                     TTATGGCTGCATTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATT
                     GTCCCCAATGTCTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATT
                     GATCCTGGGGAGCTACGAACCCAAAGTTTCACAGTAGGGAGCT"
                     /label="ATAGGTACCGAGCTCTATGCACTCACAGTGGTTTGGCATGCATCTGGTGA
                     ATTTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTCTCCCTAACG
                     TAATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGCCTCTTCCAT
                     TCTTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGTCTCTGCAAT
                     TTGTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTGTTCCTGAAT
                     CTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTT
                     TGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTG
                     CATTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATTGTCCCCAAT
                     GTCTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATTGATCCTGGG
                     GAGCTACGAACCCAAAGTTTCACAGTAGGGAGCT"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    156..427
                     /created_by="ximena.opitzaraya"
                     /label="eHGT_1129m_c1"
     misc_feature    428..699
                     /created_by="ximena.opitzaraya"
                     /label="eHGT_1129m_c1"
     misc_feature    700..971
                     /created_by="ximena.opitzaraya"
                     /label="eHGT_1129m_c1"
     misc_feature    972..1024
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey=21
                     /label="Beta\Globin\minimal\promoter"
     misc_feature    981..986
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey=21
                     /label="TATA\box"
     misc_feature    1013
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey=21
                     /label="transcriptional\start"
     misc_feature    1065..1784
                     /label="SYFP2"
     primer_bind     1111..1131
                     /label="EGFP-N-R"
     misc_feature    1819..2064
                     /label="WPRE3"
     misc_feature    2078..2281
                     /label="BGH-pA"
     primer          complement(2192..2211)
                     /label="BGH-R"
     misc_feature    2304..2444
                     /label="R-ITR"
     rep_origin      2536..2842
                     /label="f1 Origin"
     misc_feature    3361..4218
                     /label="AmpR"
     rep_origin      4369..5036
                     /label="pUC ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtaga tggatgggtg tatagattga
      181 tgggtatatg gatagaccag tgtatagatg ggtgggtggg tgaataggtg gtcttgtatt
      241 acatcctcaa gtctggtcta atccatttct cctgtaggac tggacatgtg ggtgccatat
      301 ttggcatttg ttctctaaag atagcctgga ctgttgtttt aattttgata tatatgaagt
      361 agaattctct tcttgcagta actgggtgct aaggctgagc tctcagcggg gagggccatt
      421 gtactgcgta gatggatggg tgtatagatt gatgggtata tggatagacc agtgtataga
      481 tgggtgggtg ggtgaatagg tggtcttgta ttacatcctc aagtctggtc taatccattt
      541 ctcctgtagg actggacatg tgggtgccat atttggcatt tgttctctaa agatagcctg
      601 gactgttgtt ttaattttga tatatatgaa gtagaattct cttcttgcag taactgggtg
      661 ctaaggctga gctctcagcg gggagggcca ttgtactgcg tagatggatg ggtgtataga
      721 ttgatgggta tatggataga ccagtgtata gatgggtggg tgggtgaata ggtggtcttg
      781 tattacatcc tcaagtctgg tctaatccat ttctcctgta ggactggaca tgtgggtgcc
      841 atatttggca tttgttctct aaagatagcc tggactgttg ttttaatttt gatatatatg
      901 aagtagaatt ctcttcttgc agtaactggg tgctaaggct gagctctcag cggggagggc
      961 cattgtactg cgggctgggc ataaaagtca gggcagagcc atctattgct tacatttgct
     1021 tctgggatcc agatctttcg aagctagcgc taccggtcgc caccatggtg agcaagggcg
     1081 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1141 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     1201 agctgatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg
     1261 gctacggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
     1321 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     1381 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     1441 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     1501 acaacagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact
     1561 tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga
     1621 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt
     1681 ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     1741 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaagtcgac ggcgcgccgc
     1801 ggccgcgaat tcgatatcat aatcaacctc tggattacaa aatttgtgaa agattgactg
     1861 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     1921 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttag
     1981 ttcttgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     2041 tgttgggcac tgacaattcc gtggctcgag agatcttcga ctgtgccttc tagttgccag
     2101 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     2161 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     2221 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     2281 gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     2341 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     2401 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     2461 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     2521 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     2581 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     2641 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     2701 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     2761 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     2821 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
     2881 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     2941 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     3001 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     3061 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     3121 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     3181 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     3241 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3301 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3361 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     3421 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     3481 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     3541 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     3601 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     3661 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     3721 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     3781 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     3841 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     3901 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     3961 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4021 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     4081 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     4141 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     4201 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     4261 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     4321 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4381 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4441 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4501 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     4561 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     4621 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4681 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     4741 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     4801 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4861 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4921 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4981 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5041 t
//
