LOCUS       pCMY-ZmWus2_in_p        4466 bp    DNA     circular SYN 14-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 22, 2025 from SnapGene 8.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4466
                     /note="/dnas_title=pCMY-ZmWus2 in pUC-GW-Kan"
                     /organism="synthetic DNA construct"
     misc_feature    416..879
                     /label=CMY 9.11 Promoter
     CDS             890..1798
                     /codon_start=1
                     /label=ZmWUS2 CDS
                     /translation="MAANAGGGGAGGGSGSGSVAAPAVCRPSGSRWTPTPEQIRMLKEL
                     YYGCGIRSPSSEQIQRITAMLRQHGKIEGKNVFYWFQNHKARERQKRRLTSLDVNVPAA
                     GAADATTSQLGVLSLSSPPPSGAAPPSPTLGFYAAGNGGGSAVLLDTSSDWGSSGAAMA
                     TETCFLQDYMGVTDTGSSSQWPRFSSSDTIMAAAAARAATTRAPETLPLFPTCGDDGGS
                     GSSSYLPFWGAASTTAGATSSVAIQQQHQLQEQYSFYSNSNSTQLAGTGNQDVSATAAA
                     AAALELSLSSWCSPYPAAGSM"
     misc_feature    1799..2255
                     /label=At HSP Term
     rep_origin      complement(2692..3280)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3451..4266)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRFAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagactgtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggattggca
      421 gacatactgt cccacaaatg aagatggaat ctgtaaaaga aaacgcgtga aataatgcgt
      481 ctgacaaagg ttaggtcggc tgcctttaat caataccaaa gtggtcccta ccacgatgga
      541 aaaactgtgc agtcggtttg gctttttctg acgaacaaat aagattcgtg gccgacaggt
      601 gggggtccac catgtgaagg catcttcaga ctccaataat ggagcaatga cgtaagggct
      661 tacgaaataa gtaagggtag tttgggaaat gtccactcac ccgtcagtct ataaatactt
      721 agcccctccc tcattgttaa gggagcaaaa tctcagagag atagtcctag agagagaaag
      781 agagcaagta gcctagaagt agtcaaggcg gcgaagtatt caggcaggtg gccaggaaga
      841 agaaaagcca agacgacgaa aacaggtaag agctaagctt tcctgcagga tggcggccaa
      901 tgcgggcggc ggtggagcgg gaggaggcag cggcagcggc agcgtggctg cgccggcggt
      961 gtgccgcccc agcggctcgc ggtggacgcc gacgccggag cagatcagga tgctgaagga
     1021 gctgtactac ggctgcggca tccggtcgcc cagctcggag cagatccagc gcatcaccgc
     1081 catgctgcgg cagcacggca agatcgaggg caagaacgtc ttctactggt tccagaacca
     1141 caaggcccgc gagcgccaga agcgccgcct caccagcctc gacgtgaacg tgcccgccgc
     1201 cggcgcggcc gacgccacca ccagccaact cggcgtcctc tcgctgtcgt cgccgccgcc
     1261 ttcaggcgcg gcgcctccct cgcccaccct cggcttctac gccgccggca atggcggcgg
     1321 atcggctgtg ctgctggaca cgagttccga ctggggcagc agcggcgctg cgatggccac
     1381 cgagacatgc ttcctccagg actacatggg cgtgacggac acgggcagct cgtcgcagtg
     1441 gccacgcttc tcgtcgtcgg acacgataat ggcggcggcc gcggcgcggg cggcgacgac
     1501 gcgggcgccc gagactctcc ctctcttccc gacctgcggc gacgacggcg gcagcggtag
     1561 cagcagctac ttgccgttct ggggtgccgc gtccacaact gccggcgcca cttcttccgt
     1621 tgcgatccag cagcaacacc agctgcagga gcagtacagc ttttacagca acagcaacag
     1681 cacccagctg gccggcaccg gcaaccaaga cgtatcggca acagcagcag cagccgccgc
     1741 cctggagctg agcctcagct catggtgctc cccttaccct gctgcaggga gtatgtgaat
     1801 atgaagatga agatgaaata tttggtgtgt caaataaaaa gcttgtgtgc ttaagtttgt
     1861 gtttttttct tggcttgttg tgttatgaat ttgtggcttt ttctaatatt aaatgaatgt
     1921 aagatctcat tataatgaat aaacaaatgt ttctataatc cattgtgaat gttttgttgg
     1981 atctcttctg cagcatataa ctactgtatg tgctatggta tggactatgg aatatgatta
     2041 aagataagat gggctcatag agtaaaacga ggcgagggac ctataaacct cccttcatca
     2101 tgctatttca tgatctattt tataaaataa agatgtagaa aaaagtaagc gtaataaccg
     2161 caaaacaaat gatttaaaac atggcacata atgaggagat taagttcggt ttacgtttat
     2221 tttagtacta attgtaacgt gagactacgt atcggatccc aatggcgcgc cgagcttggc
     2281 tcgagcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     2341 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     2401 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     2461 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct gttccgcttc
     2521 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     2581 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     2641 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2701 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2761 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2821 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2881 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2941 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3001 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3061 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3121 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3181 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3241 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3301 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     3361 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     3421 aagtatatat gagtaaactt ggtctgacag ttagaaaaac tcatcgagca tcaaatgaaa
     3481 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     3541 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     3601 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     3661 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
     3721 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     3781 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg aaacgaaata cgcgatcgct
     3841 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     3901 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc
     3961 agggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     4021 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     4081 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     4141 tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     4201 atcagcatcc atgttggaat ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg
     4261 gctcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     4321 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4381 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     4441 taggcgtatc acgaggccct tttgtc
//

