LOCUS       pCMY-GRF-GIF_in         5468 bp    DNA     circular SYN 14-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 22, 2025 from SnapGene 8.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5468
                     /note="/dnas_title=pCMY-GRF-GIF in pUC-GW-Kan"
                     /organism="synthetic DNA construct"
     misc_feature    416..879
                     /label=CMY 9.11 Promoter
     CDS             890..2800
                     /codon_start=1
                     /label=GRF-GIF CDS
                     /translation="MAMPYASLSPAGDRRSSPAATATASLLPFCRSSPFSAGGNGGMGE
                     EARMDGRWMARPVPFTAAQYEELEHQALIYKYLVAGVSVPPDLVLPIRRGIESLAARFY
                     HNPLAIGYGSYLGKKVDPEPGRCRRTDGKKWRCAKEAASDSKYCERHMHRGRNRSRKPV
                     ETQLVSHSQPPAASVVPPLATGFHNHSLYPAIGGTNGGGGGGNNGMSMPGTFSSALGPP
                     QQHMGNNAASPYAALGGAGTCKDFRYTAYGIRSLADEQSQLMTEAMNTSVENPWRLPPS
                     SQTTTFPLSSYSPQLGATSDLGQNNSSNNNSGVKAEGQQQQQPLSFPGCGDFGSGDSAK
                     QENQTLRPFFDEWPKTRDSWSDLTDDNSNVASFSATQLSISIPMTSSDFSAASSQSPNG
                     MLFAGEMYAAAAMQQQHLMQMNQSMMGGYASSTTATTDLIQQYLDENKQLILAILDNQN
                     NGKVEECARNQAKLQQNLMYLAAIADSQPPQTASLSQYPSNLMMQSGPRYMQQQSAQMM
                     SPQSLMAARSSMMYAQQAMSPLQQQQQQQQHQAAAHGQLGMSSGATTGFNLLHGEASMG
                     GGGGATGNSMMNASVFSDYGRGGSGAKEGSTSLSADARGANSGAHSGDGEYLKGTEEEG
                     S"
     misc_feature    2801..3257
                     /label=At HSP Term
     rep_origin      complement(3694..4282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4453..5268)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRFAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagactgtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggattggca
      421 gacatactgt cccacaaatg aagatggaat ctgtaaaaga aaacgcgtga aataatgcgt
      481 ctgacaaagg ttaggtcggc tgcctttaat caataccaaa gtggtcccta ccacgatgga
      541 aaaactgtgc agtcggtttg gctttttctg acgaacaaat aagattcgtg gccgacaggt
      601 gggggtccac catgtgaagg catcttcaga ctccaataat ggagcaatga cgtaagggct
      661 tacgaaataa gtaagggtag tttgggaaat gtccactcac ccgtcagtct ataaatactt
      721 agcccctccc tcattgttaa gggagcaaaa tctcagagag atagtcctag agagagaaag
      781 agagcaagta gcctagaagt agtcaaggcg gcgaagtatt caggcaggtg gccaggaaga
      841 agaaaagcca agacgacgaa aacaggtaag agctaagctt tcctgcagga tggcaatgcc
      901 ctacgcctct ctttcgcccg ctggcgatcg taggtcgtca cccgcagcga ctgcgactgc
      961 gtccctcctc cctttctgcc gttcatcgcc cttctcggca ggcggcaatg ggggtatggg
     1021 agaagaagca aggatggacg ggagatggat ggcaagacct gtgccgttta ccgctgcgca
     1081 atatgaagaa ttggaacatc aggcgctcat ttataaatat cttgttgccg gggtaagtgt
     1141 ccccccggac cttgtgctgc ccatccgtag gggtatagag tcacttgcgg ctcgttttta
     1201 tcataaccct ttggctattg gctatggatc gtacttgggt aagaaggtgg acccggagcc
     1261 gggcaggtgc cgtagaactg atggtaagaa gtggagatgt gcgaaggaag ccgcttcgga
     1321 ctccaaatat tgtgaacgtc atatgcatcg tgggaggaat agatctagga aaccagtaga
     1381 gactcaactg gtatcccact cccagccccc tgcggcgagc gtggtgccgc cgttggctac
     1441 cggcttccat aaccattctt tgtatccagc gattggaggc actaatggtg gagggggcgg
     1501 cggtaataac gggatgtcaa tgcctgggac attcagttca gctcttggcc ccccacagca
     1561 acatatggga aacaatgccg ctagcccgta cgcagccctg ggtggggctg gtacctgcaa
     1621 ggatttcaga tatacggcgt acggcataag atccctggca gatgagcagt cccagcttat
     1681 gactgaggcc atgaacacca gtgtcgagaa cccatggcgt ttgcctcctt caagccaaac
     1741 gacaaccttt ccgttgagct catattcacc ccagctcgga gcaacgtcgg acctcggtca
     1801 aaacaactcc agtaataata atagtggtgt taaagcggaa ggacagcagc agcagcaacc
     1861 gctttcattc cctggctgcg gtgactttgg atccggggat tcggcgaagc aagaaaatca
     1921 gacgctcaga cccttctttg acgagtggcc aaagacgaga gactcatggt cggatcttac
     1981 agacgataac tctaatgtcg cgagtttctc cgccacacag ctcagtatat cgattcccat
     2041 gacttcatcg gattttagcg ccgcaagttc tcaatcgccc aacggaatgc ttttcgctgg
     2101 cgaaatgtat gctgccgccg cgatgcagca gcagcatctc atgcagatga accagtcaat
     2161 gatgggtgga tacgctagca gtacaactgc aaccacggat cttattcaac agtatttgga
     2221 tgaaaataag caacttatat tggctattct ggataaccag aataatggta aggtcgaaga
     2281 gtgcgctcgt aatcaagcta agctccagca gaatctcatg tatctcgcag ctatagctga
     2341 ttctcagcct cctcagactg cttccctctc tcagtatccc agcaacttga tgatgcaatc
     2401 tggcccaagg tatatgcagc aacagtccgc acaaatgatg agcccccaat cgttgatggc
     2461 ggcgcgttcg tccatgatgt atgcgcaaca ggcaatgagt cctttgcagc aacaacagca
     2521 acaacaacag catcaggcgg ccgcacacgg acagctcgga atgtcttcgg gtgctaccac
     2581 cggattcaac ttgcttcatg gtgaggcgtc tatggggggc ggtggggggg cgacgggcaa
     2641 ttctatgatg aacgcttcgg ttttctcgga ctacgggcgt ggtggaagtg gcgctaaaga
     2701 aggttccacc tccctgagtg cggacgcaag gggagccaat tccggtgcgc attctggcga
     2761 tggggaatac cttaaaggta ctgaggagga aggaagctga atatgaagat gaagatgaaa
     2821 tatttggtgt gtcaaataaa aagcttgtgt gcttaagttt gtgttttttt cttggcttgt
     2881 tgtgttatga atttgtggct ttttctaata ttaaatgaat gtaagatctc attataatga
     2941 ataaacaaat gtttctataa tccattgtga atgttttgtt ggatctcttc tgcagcatat
     3001 aactactgta tgtgctatgg tatggactat ggaatatgat taaagataag atgggctcat
     3061 agagtaaaac gaggcgaggg acctataaac ctcccttcat catgctattt catgatctat
     3121 tttataaaat aaagatgtag aaaaaagtaa gcgtaataac cgcaaaacaa atgatttaaa
     3181 acatggcaca taatgaggag attaagttcg gtttacgttt attttagtac taattgtaac
     3241 gtgagactac gtatcggatc ccaatggcgc gccgagcttg gctcgagcat ggtcatagct
     3301 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     3361 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     3421 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     3481 cgcggggaga ggcggtttgc gtattgggcg ctgttccgct tcctcgctca ctgactcgct
     3541 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     3601 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     3661 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     3721 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     3781 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     3841 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     3901 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3961 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4021 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4081 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4141 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4201 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4261 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4321 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4381 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     4441 ttggtctgac agttagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc
     4501 aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc
     4561 gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac
     4621 atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc
     4681 atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg
     4741 ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt
     4801 cattcgtgat tgcgcctgag cgaaacgaaa tacgcgatcg ctgttaaaag gacaattaca
     4861 aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc
     4921 tgaatcagga tattcttcta atacctggaa tgctgttttc ccagggatcg cagtggtgag
     4981 taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc
     5041 cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc
     5101 atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc
     5161 tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga
     5221 atttaatcgc ggcctagagc aagacgtttc ccgttgaata tggctcatac tcttcctttt
     5281 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     5341 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     5401 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     5461 cttttgtc
//

