LOCUS       Exported               12066 bp ds-DNA     circular SYN 22-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12066)
  AUTHORS   The Weissman lab
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 6, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12066
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          6495..6524
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /note="/locus_tag=source:synthetic DNA construct"
     intron          20..85
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             215..235
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    660..794
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             963..1596
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1643..1768
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2265..2497
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2682..2726
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3011..3128
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    3188..3775
                     /label=UCOE
     promoter        3870..4277
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      4311..4316
                     /regulatory_class="other"
                     /locus_tag="vertebrate consensus sequence for strong
                     initiation  of translation (Kozak, 1987)"
                     /label=vertebrate consensus sequence for strong
                     initia...
                     /label=vertebrate consensus sequence for strong
                     initiation  of translation (Kozak, 1987)
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
     misc_feature    4320..4982
                     /locus_tag="mCherry"
                     /label=mCherry
     misc_feature    4983..5048
                     /locus_tag="P2A"
                     /label=P2A
     misc_feature    5049..5114
                     /locus_tag="3xFlag"
                     /label=3xFlag
     misc_feature    5118..5345
                     /label=BirA
     misc_feature    5343..5345
                     /label=L76
     misc_feature    5346..5762
                     /locus_tag="hLOV"
                     /label=hLOV
     misc_feature    5763..6485
                     /label=BirA
     misc_feature    6537..6821
                     /locus_tag="Sec61B"
                     /label=Sec61B
     misc_feature    6825..6827
                     /locus_tag="Sec61B"
                     /label=Sec61B
     misc_feature    6828..6831
                     /locus_tag="rtTA3 promoter region"
                     /label=rtTA3 promoter region
     misc_feature    6891..7479
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(7362..7373)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7482..7498)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             7589..7822
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7847..7865)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        8653..8757
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8758..9618
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9789..10377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        10623..10952
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10803..10938
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
       61 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
      121 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
      181 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
      241 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
      301 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
      361 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat
      421 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa
      481 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag
      541 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
      601 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
      661 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
      721 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
      781 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
      841 cataccctat taccactgcc aattacctag tggtttcatt tactctaaac ctgtgattcc
      901 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
      961 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
     1021 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
     1081 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
     1141 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     1201 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
     1261 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
     1321 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
     1381 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     1441 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     1501 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     1561 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     1621 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     1681 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     1741 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1801 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1861 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1921 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1981 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     2041 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     2101 gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     2161 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     2221 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     2281 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     2341 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     2401 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     2461 ctggctgtga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     2521 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     2581 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     2641 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     2701 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     2761 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     2821 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     2881 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     2941 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccaa atggcagtat
     3001 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     3061 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     3121 aaaattttcg ggtttattac agggacagca gagatccagt ttggatcgat aagcttgata
     3181 tcgaattcgg gaggtggtcc ctgcagttac gccaatgata acccccgcca gaaaaatctt
     3241 agtagccttc cctttttgtt ttccgtgccc caactcggcg gattgactcg gccccttccg
     3301 gaaacacccg aatcaacttc tagtcaaatt attgttcacg ccgcaatgac ccacccctgg
     3361 cccgcgtctg tggaactgac ccctggtgta caggagagtt cgctgctgaa agtggtccca
     3421 aaggggtact agtttttaag ctcccaactc cccctccccc agcgtctgga ggattccaca
     3481 ccctcgcacc gcaggggcga ggaagtgggc ggagtccggt tttggcgcca gccgctgagg
     3541 ctgccaagca gaaaagccac cgctgaggag actccggtca ctgtcctcgc cccgcctccc
     3601 ccttccctcc ccttggggac caccgggcgc cacgccgcga acggtaagtg ccgcggtcgt
     3661 cggcgcctcc gccctccccc tagggcccca attcccagcg ggcgcggcgc gcggccccgc
     3721 gcgcagctcc cggctccctc ccccttcgga tgtggcttga gctgtaggcg cggagtcctg
     3781 cagccccgat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
     3841 ccacctgtag gtttggcaag ctagctgcag taacgccatt ttgcaaggca tggaaaaata
     3901 ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag ctaacgttgg
     3961 gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa gaacagatgg
     4021 tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga tatggcccaa
     4081 ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg acctgaaatg
     4141 accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt cgcgcgcttc
     4201 tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca gtcctccgac
     4261 agactgagtc gcccgggggg gatctggagc tctcgagaat tctcacgcgt gccaccatga
     4321 acatggcgat tatcaaggaa ttcatgaggt tcaaagttca catggaggga tctgtaaacg
     4381 gccacgagtt cgaaatcgag ggagaggggg agggtcgacc atatgaagga acacagaccg
     4441 ctaaattgaa ggttactaaa ggtggaccac ttcctttcgc atgggacatt cttagccccc
     4501 agtttatgta tggttccaag gcgtatgtca aacatcccgc agacattcct gattacctta
     4561 aactgagttt tccagagggg ttcaaatggg agcgcgtcat gaactttgaa gatgggggcg
     4621 ttgttacagt gactcaggac tcttctttgc aagatggaga gtttatatat aaggtgaaac
     4681 ttcgcggaac caacttccca agcgatggcc cagtcatgca gaagaaaact atgggttggg
     4741 aagccagtag cgagcggatg tacccagagg acggcgcact taagggtgag attaagcagc
     4801 ggctgaaact caaagacgga ggccattatg acgcggaagt taaaacgacg tacaaagcta
     4861 aaaagcctgt ccaattgcca ggcgcgtaca atgtcaatat taaattggac attaccagtc
     4921 acaacgaaga ttacacaata gtggagcaat acgaacgcgc agagggacgc cattcaacag
     4981 ggggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc
     5041 ctggacctga ctacaaagac catgacggtg attataaaga tcatgacatc gattacaagg
     5101 atgacgatga caagcttatg aaggataaca ccgtgccact gaaattgatt gccctgttag
     5161 cgaacggtga atttcactct ggcgagcagt tgggtgaaac gctgggaatg agccgggcgg
     5221 ctattaataa acacattcag acactgcgtg actggggcgt tgatgtcttt accgttccgg
     5281 gtaaaggata cagcctgcct gagcctatcc agttacttaa tgctaaacag atattgggtc
     5341 agctggagtt tcgggcaacc acactggaac ggatcgagaa aagcttcgtg attactgatc
     5401 cgagactgcc tgacaaccca atcatttttg tgagcgattc cttcctgcag ctgacagaat
     5461 attctcggga agagatcctg gggcgcaatt gccgttttct gcagggaccc gagacagacc
     5521 gtgccactgt tcggaaaatc agagatgcta ttgacaacca gactgaagtg accgttcagc
     5581 tgatcaatta taccaagagc ggcaagaagt tctggaacgt gttccacctg cagccgatgc
     5641 gcgattataa gggcgacgtc cagtacttca ttggcgtgca gctggatggc accgaacgtc
     5701 ttcatggcgc cgctgagcgt gaggcggtct gcctggtcaa aaagacagcc tttcagattg
     5761 ctgtagccgt gctgccagtg attgactcca cgaatcagta ccttcttgat cgtatcggag
     5821 agcttaaatc gggcgatgct tgcattgcag aataccagca ggctggccgt ggtcgccggg
     5881 gtcggaaatg gttttcgcct tttggcgcaa acttatattt gtcgatgttc tggcgtctgg
     5941 aacaaggccc ggcggcggcg attggtttaa gtctggttat cggtatcgtg atggcggaag
     6001 tattacgcaa gctgggtgca gataaagttc gtgttaaatg gcctaatgac ctctatctgc
     6061 aggatcgcaa gctggcaggc attctggtgg agctgactgg caaaactggc gatgcggcgc
     6121 aaatagtcat tggagccggg atcaacatgg caatgcgccg tgttgaagag agtgtcgtta
     6181 atcaggggtg gatcacgctg caggaagcgg ggatcaatct cgatcgtaat acgttggcgg
     6241 ccatgctaat acgtgaatta cgtgctgcgt tggaactctt cgaacaagaa ggattggcac
     6301 cttatctgtc gcgctgggaa aagctggata attttattaa tcgcccagtg aaacttatca
     6361 ttggtgataa agaaatattt ggcatttcac gcggaataga caaacagggg gctttattac
     6421 ttgagcagga tggaataata aaaccctgga tgggcggtga aatatccctg cgtagtgcag
     6481 aaaaaggagg ttctggtgga ggcgggtctg gaggcggggg tagtaacagc cgcgtgcctg
     6541 gtccgacccc cagtggcact aacgtgggat cctcagggcg ctctcccagc aaagcagtgg
     6601 ccgcccgggc ggcgggatcc actgtccggc agaggaaaaa tgccagctgt gggacaagga
     6661 gtgcaggccg cacaacctcg gcaggcaccg gggggatgtg gcgattctac acagaagatt
     6721 cacctgggct caaagttggc cctgttccag tattggttat gagtcttctg ttcatcgctt
     6781 ctgtatttat gttgcacatt tggggcaagt acactcgttc gtagtaaggc cgcggccgcg
     6841 actctagagt cgacctgcag gcatgcaagc ttgatatcaa gcttatcgat aatcaacctc
     6901 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6961 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     7021 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     7081 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     7141 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     7201 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     7261 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     7321 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     7381 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     7441 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
     7501 ggaattaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     7561 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     7621 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7681 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7741 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7801 tcagtgtgga aaatctctag cagcatctag aattaattcc gtgtattcta tagtgtcacc
     7861 taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt tctaacgaca
     7921 atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta tcatctctct
     7981 cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct ggtaacgccg
     8041 tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc cagatcctct
     8101 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
     8161 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
     8221 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
     8281 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
     8341 taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct
     8401 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     8461 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     8521 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     8581 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     8641 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     8701 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     8761 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     8821 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     8881 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     8941 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     9001 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     9061 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     9121 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     9181 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     9241 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     9301 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     9361 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     9421 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     9481 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     9541 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     9601 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     9661 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     9721 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     9781 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     9841 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     9901 actggcttca gcagagcgca gataccaaat actgtctttc tagtgtagcc gtagttaggc
     9961 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    10021 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    10081 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    10141 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    10201 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    10261 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    10321 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    10381 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    10441 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    10501 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
    10561 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga
    10621 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
    10681 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
    10741 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
    10801 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
    10861 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
    10921 gaggcttttt tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag
    10981 atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg
    11041 gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt
    11101 ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa
    11161 aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg
    11221 atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg
    11281 cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg
    11341 atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct
    11401 tcatcgttat tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt
    11461 atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg
    11521 actatgttgt tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg
    11581 tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg
    11641 ttctttttaa cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc
    11701 atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc
    11761 tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat
    11821 ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc
    11881 attgacccac gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt
    11941 accgacgatg atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag
    12001 tgggccccgg atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact
    12061 acctac
//
