LOCUS       Exported               19527 bp ds-DNA     circular SYN 22-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19527)
  AUTHORS   The Weissman lab
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 6, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..19527
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    874..936
                     /label=5'ITR
     misc_feature    complement(1400..1993)
                     /label=WPRE
                     /label=WPRE; Woodchuck post-transcriptional
     primer_bind     complement(1893..1912)
                     /label=HKO083
     CDS             complement(2016..2615)
                     /label=Puro (-)
     promoter        complement(2628..3130)
                     /label=PGK (-)
     misc_feature    3137..16893
                     /label=NDUFB9-endogenous locus
     misc_feature    5066..5150
                     /label=5'UTR
     misc_feature    9217..9228
                     /label=NDUFB9
     misc_feature    9217..9228
                     /label=variant_bps
     misc_feature    9217..9228
                     /label=variation
     misc_feature    15857..15950
                     /label=3'UTR
     misc_feature    16981..17015
                     /label=3'ITR
     primer_bind     complement(17506..17522)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    17530..17546
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(17554..17584)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    17599..17620
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(17908..18496)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(18667..19527)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc agggtttatt acagggacag cagagatcca
     1381 gtttggttaa ttagctagcg atgcggggag gcggcccaaa gggagatccg actcgtctga
     1441 gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg
     1501 tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg
     1561 gcaacacagg cgagcagcca aggaaaggac gatgatttcc ccgacaacac cacggaattg
     1621 tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc
     1681 gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca
     1741 atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg
     1801 acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa
     1861 atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat
     1921 agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc
     1981 agaggttgat taccgataag cttgatatcg aattcttaac tggcaccggg cttgcgggtc
     2041 atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc
     2101 cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg
     2161 cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg
     2221 tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc
     2281 aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc
     2341 gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc
     2401 acggcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg
     2461 aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc
     2521 gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg
     2581 cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg tggtaccccc tggggagaga
     2641 ggtcggtgat tcggtcaacg agggagccga ctgccgacgt gcgctccgga ggcttgcaga
     2701 atgcggaaca ccgcgcgggc aggaacaggg cccacactac cgccccacac cccgcctccc
     2761 gcaccgcccc ttcccggccg ctgctctcgg cgcgccctgc tgagcagccg ctattggcca
     2821 cagcccatcg cggtcggcgc gctgccattg ctccctggcg ctgtccgtct gcgagggtac
     2881 tagtgagacg tgcggcttcc gtttgtcacg tccggcacgc cgcgaaccgc aaggaacctt
     2941 cccgacttag gggcggagca ggaagcgtcg ccggggggcc cacaagggta gcggcgaaga
     3001 tccgggtgac gctgcgaacg gacgtgaaga atgtgcgaga cccagggtcg gcgccgctgc
     3061 gtttcccgga accacgccca gagcagccgc gtccctgcgc aaacccaggg ctgccttgga
     3121 aaaggcgcaa acgcgtccca aacagtcatt gtactacgcc cctaggaaaa aaatataaat
     3181 accataccag tatctgcctt aagagtttga tctagtggga aaggcatact catatttaat
     3241 tataaataat gtggtataag ttttaaggag tgagtaggca gacaggcaac agggtagttg
     3301 gtaggcagag gaatattttg gccccaaact gccactctaa aaccttgtca gaggtaacaa
     3361 acagcatgca caagtgttta tagagcagcc cagggatggg ctagaaaagt gggcaggact
     3421 ggaccacaga agggctttgt atggtgtgta agcatttagg ctatcctgag gagcgtaaat
     3481 gaaagatttg aagcaaaaga aacatttagc tgatttgatt tttagataca ccaaaatgga
     3541 gtctacatgg aggatggatt taagacatta acttgaggca ggaatttggt tgtataacat
     3601 aaacacactt ttaaaaagtt atgtggcctt tctttacgga gtacttcatg gctctttatt
     3661 ccatctccct catcaattat atcaaaggtt aggatggaaa ggctaagaca cggcaatgtc
     3721 ttatttgaca acccctcccc aaacccctac tccaggcttt cccacagcaa tttcaaaacc
     3781 tttttcctgg cacttaacat attctcccct gcatttgctt ttgactgtac agtacatagg
     3841 tcttaggtct ccagtctgaa aactgcacca ctagatttta cagttccaca ggacttagca
     3901 caacgtcttt gaaaaaaaaa aatactgctt aaataactct cagatctaaa tgtataatcc
     3961 taatctcttg attgccagac cacattccca attgcccaga tatctctacc tggatgtcct
     4021 ataattatct caaacttaac ctaatccaaa tggacctggg tacctctctt caccgccaaa
     4081 ctagtcatgc ttccatttca tatttaagta cttttccaaa ccctgagtca tctttgttca
     4141 ctctctacta ccggccatga acactcagct gctaaatgct gcacacatct cttccatttc
     4201 tctctctttt ccattaccct agtttaagct cttgaaatgc ttccctacct ctccctattt
     4261 cccacaaaac ctacataata gctatcagga ttaccttgct aaagcagagg tgtgatcact
     4321 gtcatttccc accttcaggt agctggaaac cccacaaaac gaggatcatg cccatttaaa
     4381 tcccgcagca ttctccctct cgttctactg atgccgactg gatcattgta cttgccaagt
     4441 acagtttgtt tttctcccca ttacttgtgt tttctagttt ctccaccaga tggtgatgac
     4501 agtacaactg ggagctttgt gccctgtgtt cctaggccag tgccaggccc acttgaggcg
     4561 ctcactaact atgatatgaa cgaatgaatg cttgagtgaa ttggtcccca gaagcggtca
     4621 gcctctccgc caaggcagag gggaagggcc gggaggtgca cgtgggatga atggaaacat
     4681 agtgattggc ctttttggag ttttcttctt aaaaaactga atacattaca gatgttgaaa
     4741 acagcacaaa aatcataact gtacggcttg ttaattttta caaataaata caccctcgtg
     4801 aatccagaac ctagatcaag aaccagagca ttaccagcac ccagaaacct ccccgcgccc
     4861 tcctccactg agcggccctt tcaaccctta caatgccgga gggcgcaatt cctgggtcct
     4921 gtgaccttgg tgaccgacgc ccgggacaag tcagaaagac ccaagggcgt ggaaaacgct
     4981 cctcttaccg ataaacttga agcgactcat gactgcgcat ggaggacctc cccagcggaa
     5041 gcggaagtgg ccgccggcaa ctccgccctt ccggctggcc ccgctcagtc acccgcagca
     5101 ggcgtgcagt ttcccggctc tccgcgcggc cggggaaggt cagcgccgta atggcgttct
     5161 tggcgtcggg accctacctg acccatcagc aaaaggtgtt gcggctttat aagcgggcgc
     5221 tacgccacct cgagtcgtgg tgcgtccaga ggtaagggat ggggacccag gactcgggga
     5281 ggtgaccctc ggggccccat ggaggtggag aggcccctgg gtacctggag gttcaaggac
     5341 ttcgggaacg gaattggaag gtggcctctc gcttcacaga atcctatggt agacccgggt
     5401 tcaaatccag gcttcgccac ttatggccgg gagaccttgt agagttgctt gtcgggtctg
     5461 ggcgtcagct ttttgtttag taaaacggac gcacgaccag cgcagagggt cgcaggaaaa
     5521 ttaagtgaaa taaaggaaag tgcctaaaat tgtaattgtc atgggtccag cgctcgctca
     5581 atcgttttct tcacgatctt ctcctttcca atattaacag caataaacta tgcattattt
     5641 tcttcagtct gcaaaactct aggagttagg aattatctta agcttatttt acagataaga
     5701 agatgagcac agctggtaac ttgcgcaagg tgatagagtt tctaagtgtc tgagccaggg
     5761 ttaaaattta ggtctttctc caaagcatca tcattctccc acccctccca ttagcttttc
     5821 tctttttagc ttcatctagt ccttttcatt acctttacat ctttttcacc tcttacataa
     5881 ttctctttcg acctctatcc gtctgagact agtctgagaa catagtgtta aaatgtgtag
     5941 attttaatca cctaaattca ttgcagggtg ggctaacaac ccaagatgtg tgagtgcctc
     6001 gcctccactc cagtcttacg actctccaaa cgtagtttta taagaaggcc ttctgggaaa
     6061 aaaactctat ggaaaatgga gtaggcctgg aagaattaca gagggtaccc tcgaagccca
     6121 gattgcatat atgtgactat tagaaaatca ctgacatact tctcttgatt tggcaggaag
     6181 atggccaagt acaatgtgat gggattgtta gtttcttcct tgagttttcc ttatttattt
     6241 attgatttga cagaatttgc aattcagatt ctatgctcta tgctaaatat ataaatgtta
     6301 gtaaatccat ttttgagcag cagcagctca cggattagtg gggaaaatgg acatgtaaac
     6361 aaatcgatga tgaaggaagt tgagccatca gggattcagg tatcccaaca caaatggtaa
     6421 atgtttgagg tgatgaatat ccaaattacc ctgatttgat cattatacat tatatggatg
     6481 tattaaaata tcacatgtac cccataaata ggtacaataa ttatgtgtca aaaaaagtta
     6541 aacatgaaaa aaagaattca agcaatactg aactatagct gggaatacta aagtccatag
     6601 ttctagagga aaatgatata aactagctac tatgacagtt atggctcagc attttttaga
     6661 tctgacattc tttctcaatc cttgccttaa tttcttcctc catttgccct cattgtgctg
     6721 ttctctgtgg ttccatccta ggcatactgt gttgttgtat gtactcgggc agcctcattt
     6781 agtgctgtca ccacctgcgc tcatttgtct ctagagctgt agttcgaatt gcttgttaga
     6841 tcacgactgt tggctgggca tgatggctca cgcctataat cccagtactt tgggaggctg
     6901 aggtgagtgg atcacctgag gccaggagtt caacagcctg gcctacatgt gaaaccgcgt
     6961 ctctactaaa aatacaaaaa ttagccaggc gtggtggtgg gtgcctgtaa tcccagctac
     7021 tcatgaggct gaggcagaag aaccgcttga acctgggagg cagaggctgc agtgagccga
     7081 gatagcccca ttgcactcca gcctgggcga caagagtgaa actttgtctc aaaaaataat
     7141 aataaaaaaa aaattgaaaa gatcaccaac cgttctctca atctccattt atctaaattc
     7201 tttgaaatca aacttgtttt cctgtcactc cccattctga ctcttttccc aaattccctg
     7261 cctccgctaa tggtattatt agtaattcag ttactgagct ttaagtctca gaattgttct
     7321 caagtatttg ctccttattc ctttgccaat aacacacata ctcatacacc atgtataatt
     7381 agacactggt ttttataaat tctctttcca gtatgatatt agtaactgac attatttaac
     7441 acttgggatg taccaggcac tttttaaagt gtgtttatct tattgactgt ttatgacaac
     7501 cttaagatat acacactgtt attgccactg ttttacagag gaggaacccg tggcctagaa
     7561 gacttttttt gtttgttttt ttgttagacg gagtttcgct tatgttgccc aggctggagt
     7621 gcagtggcgc gatcttggct caccgcaagt tccgtctccc gggttcaagc aattctcctg
     7681 cctcagtctc ccgagtagct gggattacag gcatgcgcca ccacgcctgg ctaatttttg
     7741 tatttttagt agagacgggg ttttgccatg ttggccaggc tggtcttgaa ctcctgacct
     7801 caggtgacct ccacctcagc ctcccaaagt gctgggatta caggcgtgag ccaccgtgcc
     7861 tggccttttt gttgttgttg ttgttgtttt tttttgagat gaagtctcgc tcttgtcccc
     7921 caggctggag tgcaaatggc acaatctcga ctcactgcaa cctctgcctc ccgggttcaa
     7981 gctattctcc tgccttggcc cccagagtag ctgggattac aggtgcctgc caccacgccc
     8041 ggctagtttt tgtattttta gctgagacgg gatttcacca tgttggccag gctggtgtag
     8101 aactcctcac ctcagatgat ccccctgcct tggcctccca aagtgctggg attacaggcg
     8161 tgagccacca tgcccggcct catttttgta tttttagtag agacggggtt tctccatgtt
     8221 gatcaggctt gtcttgaact ccagacctca ggtgatccac acgccttggc ctcccaacgt
     8281 gctgggatta caggtgtgag ccaccgcacc tgaccagaag acagtaactt accccaagtt
     8341 acacagctag tagtggcaga gctaagattc aggcccatac agtctgactg gagagcctat
     8401 tctcataact tggaagtctc aatgtctccc ctgtctcctg tcaccatcac agctccgtac
     8461 tttcaggccc tcgtcagctg tgtcctagat tattgtcaaa acctcctaac tgatctttgc
     8521 ccagtttctt tagaacttga ctttaacttg cctaagttta tgcagctagt gtcagagtca
     8581 ggatacaaac ctagctctgt atgagctaag caatactgtt tctgccaagt aacacctttc
     8641 ttattcatct ttgtatctcc agaatttagc atagtgacta gcacaagata gaaggaaatg
     8701 gaagttgtca ttcaatgaat gttgaatgat cctgcagagg ttatagcttt tctcttgcga
     8761 atgctctttt cttttttttt tttttttttt ttgtttaaat cctcattcca ttgttggatg
     8821 taagaggaga aaaatggtta gggtatatat ttaatacagt atttttttgc tgctgctgtt
     8881 tggtgggctg tagaggaagc agccatgatg atacggctgc tccattggga gggttggggt
     8941 aggtccactg cccatccaga aggtatcaga agtgcagcct cctgtcagac agcaacactg
     9001 accacgtgag taggtaacat ttctaatctt caaaatcatt ttggtttaag agacaaatac
     9061 cgatactttg cttgtttgat gagagcccgg tttgaagaac ataagaatga aaaggatatg
     9121 gcgaaggcca cccagctgct gaaggaggcc gaggaagaat tctggtaccg tcagcatcca
     9181 cagccataca tcttccctga ctctcctggg ggcaccagct atgagcggta cgattgctac
     9241 aaggtaggtg agaattatga tgactgcctt ctgagaaata gactttgttt cttcagtccc
     9301 acacctcagc ctcagaaatc acttgccctt caaggggtag ctaggagact tatttcctga
     9361 cagttgtcag atgcaggcct acctcatttt attgcatttt gttttattgt gcttggcaga
     9421 tattgtattt tcacagattg aaagtttgtg gcaaccccgt gctgagcaag tctgtgggca
     9481 ccatttttcc aacagcatgt gctcactccc tgtctctgtt tctcagtttg gcaattcttg
     9541 caatatttca aacttttttt tattattact ctatctacac acagttgtgg tgaactgtga
     9601 tcagtcacct ttgatgttac tattgtcatt gttttggggc acaaaaaaac catgcccaca
     9661 taagacagca gacttaatcg ataaatgtgc gtgtgctgac tgtttcaccg accaatagtt
     9721 cctccgcctc tatccctctc ctcagggttc cctattccct aagacgcagc aacagtgaaa
     9781 ttaggacaat taacgaccat accatagcct ctaagtgttc aagtgaaagg aagagttaca
     9841 tgtctctcac tttaaatccg ttacacgtct cttcctttaa atcaaaagct agaaatgatt
     9901 aagcttagtg aggaaggcat gttgaaagcc aaggtgggta gaaagcaagg ccttttccgc
     9961 tggttagcca agttgtgaat gcaaaggaaa ttaaaggttt ttttaaggaa attaaaagtg
    10021 ctgctccact aaacacacaa atgataagtg aaactgtctt attgctgata tggagaaagt
    10081 tttagtggtt gggacaaaag atcaaaccag ccacgacatt cccttaagcc aaagcctaat
    10141 ccaaagcaag tctttaactc ttcatttcta tgaaggctga gagagatgag gaagctgcag
    10201 aaaaaaagtt ggaagctaat agaggttggt ccatgaggtt taaggaaaaa agccatctct
    10261 gtaacacaga tgtgcaaggt gaagcaacaa gtgctgatgg aaaagctgca gcaaattatt
    10321 acacaaaatc tggctaagat agttgatgaa ggtggctata cttaacaaca gattttcagt
    10381 ggagatgaag tagccttttt ttggaataag ataccatgta gaactttcat accttgagag
    10441 aaatcaatgc ttggcttcaa aacttcaagg ataggctgac tctcttgtta gggactaatg
    10501 cagttggtgg ttttaagttg aagccattgt tcatttgcca ttctgaaaat cctgaggccc
    10561 ttaagaatta tgctaaacct actctgcctg tgccctagaa atggaataac aaagcctgaa
    10621 ttacagcaaa tctgtataca acatggttta ctgaatcttt taagcccatt gttgagacct
    10681 gctaccatgt ctgctaacac aacatccatt ctgcagccca tggatcaagg agtaatttcc
    10741 acttgcaagt tttattattt aagaaatata ttttgcatag ttatagttgc catagatagt
    10801 gattcctctg atggatttgg gcaaagtaaa attttctgga aagaattcac cattctaaat
    10861 gccattgagg atatttatga ttcatgggaa gagatcaaaa tatcagcttt aacggggttg
    10921 gaagaagttg attccaaccc ttgtgggtga ttttgaaggg ctcaagactt cagcagaaga
    10981 agtaactgca gaagttgtgg aaatagcaag agagccagaa tagaagctga tcctgagaat
    11041 atgactgaat tgctgtaatc tcatgataaa tcttgagcag atgaagagtt gcttcttatg
    11101 gatgaatacg aaagtggttt cttgagatgg agtctcctgg tgaagttttt gtgaacattg
    11161 atgataacaa aggacttcgc atagcacatc aacttagttg attaagcagt agcagggttt
    11221 gagaggattg actccaattt tgcaagaagt tcttctgtgg gtaaaatgct atcaaatggc
    11281 atcacatgct atagaagtgt ttggtgtaag gaagagtcag ttgatgtcgc aaacctcgtt
    11341 attttaggaa attgccaaag ccactccagc ctttggcaat catcaccctg attagtcacc
    11401 agccatcaac attgatataa tactctccac cagtaaaaag attggttttc tgatggctca
    11461 gatgattgtt ggcagttttt atcaataacg tatttctttt gtttttgttt gggagatggt
    11521 ctccctctgt tgcccaggct ggagtgcaat agcatgatca tagctcactg cagcctggat
    11581 cccctgggct caagcaatcc tcctgtccta gccaaccaag tagctaagaa aacagaggca
    11641 caacaccaca cctagctaat ttattttatt ttttgtagag atggaatctt gctatgttgt
    11701 ccaggctggt ctggaactcc taccctcata gaatctccct gcctcagcct tcaaagggct
    11761 gggactaaag gcaggagcca cctcacccaa ccaataatgt atttttaaat tatgtctttt
    11821 ttttggtgga gggggacaga gttttgccca ggctggagtg caatggcaca atctcggctc
    11881 accgcaacct ccacctccca ggttcaagcg attctcctac ctcagtctcc caagtagctg
    11941 ggattacagg catgtaccac tatgcctggc tagttttgta tttttagtag agacagcgtt
    12001 tctccatgtt ggtcaggctg gtctcgaact cctgacctca ggtgatccgc ctgcctcagc
    12061 ttcccaaagt gctgggatta caggcgtgag ccactgcacc tggcctaaat tatgtacatt
    12121 ttttatgtat attatgtgag gtttgccaca tcaattgact tttccttttg atagcatttt
    12181 acccacagaa gaacttcttt caaaatggga atcaatcctc tcaaaccctg ccactgcttt
    12241 atcaactaag ttgatgtact gttcaaagtc ctttgttatc agttaatcaa tattcacaaa
    12301 atcttcacca ggaggactcc atctcaacag acatcataat gctgttggac actttatagt
    12361 atagtataaa caaaactttt atatgcactg ggaaaccaaa aacattttgt gacttgcttt
    12421 attgtgatat ttgctatatt gcagcggtct ggaaccaaac ccccagtatc tctaaggtgt
    12481 gcctgtactg tttcttaggt gtcagttttc tctctctgag acgtcattaa gacttttctt
    12541 tctacattca gatttaaggt cgtacagtat actttttgct ataacaaagt tcattttgga
    12601 ctttaaagtt aaccaacttg gattggtgct taatatttct gagatactct aattaagagt
    12661 tccaagtaag ataagactac tctggatatt tagagaaggc aggtagggga ggaatgaagc
    12721 cagttctcct tggactgatg atggtgctat ttttcagcac agtctttgac tctaaggtgt
    12781 tttcctttat ctttgcttcc tacttatcct ctgctgttcc ttgcttagag ttagaaacag
    12841 tctggtagtt gcagtggtta taaaaatata tcttgatctt tgtcttacat ctcctgagca
    12901 ggccctgtca gggataggta aatgtgtcct gtggattgat accatgattt cctctcctga
    12961 caggtcccag aatggtgctt agatgactgg catccttctg agaaggcaat gtatcctgat
    13021 tactttgcca agagagaaca gtggaagaaa ctgcggaggg aaagctggga acgagaggtg
    13081 cgttctactt gtacttcgtt attccttagc tatgtagatg gagtgaagtt ttgctcccca
    13141 caagcagagg tccagattcc agccatgaca gttactctgg gcccttgctt ctcagatata
    13201 ctttagtatc taagtgtatc aatttaacat ttataatcac cttacaagta agttaaagtg
    13261 cattccctgg tcgttttggc tttgtctttg attttgcttc agatctcctt ctctttagtc
    13321 ctttgtctga cttttttgcc ttcttgctat actttccctc aacctgcctc tccactattt
    13381 tcttaggcat ttttcagtca tgttaggatg gtcagttata tttttaaaag ctcaatcttg
    13441 ctgtagtgtg aagaatatat tggagagggg aagaggagaa gcagagagac ccttctagga
    13501 ttattgcaga agtttagtca aaaggttgtc atgactaaca ctagggtgga tgaagtggat
    13561 atggaaggaa attgactcag gacatcattt ggagggatgt gacgactgac tggatgttga
    13621 gggtgggtga gggatgggga ggaatcaaga atggtttctg gggcagggtg cggtggctca
    13681 cacctataat cccagcactt tgggaggcca aagtgggtga tagcttgagg tcaggagttc
    13741 atgacgacca gcctgactaa catggtgaaa ccccatctct actaaaagta caaataatta
    13801 gctgggcgtg ggggctgtgc acctgtaatc ccagctactt gggaggctaa ggcaggagaa
    13861 ttgcttgaat cccaggaggt ggaggttgca atgagctgac atcgcgccac tgcattccag
    13921 cctgggcgac agagcaagac tccatcccaa aagaaaaaaa aaagagagaa tggtttctgg
    13981 gttttaagct ttaacatctg gagtttggtg atgtttactg agatagggaa gattggggaa
    14041 gggggcacag gtgtcagagc agcaatcgag aattcatttt ggaagtgtga agttttgaga
    14101 atcctgtgag acatcaagtg cagatgtcag aagccaactt cagatgtgga aatctggaac
    14161 tcccaggaga gatttggcct agaaacagaa acgtgggagt tttcagtttt aaaagccatg
    14221 gagagatgta tggaatcctt aggagagagt atagagaaaa aagaaaagga aaaaggattc
    14281 tttgtgcatt ataattgggc aaaagattgt atataaatac atttgggtta gaagttttga
    14341 agatgacaga cttgtgtgat agcttccttt ttttagtgaa ataagaggaa aagcccagaa
    14401 tgagtgggtg cagcatattt ggagaatgtg gacaagatgt gatagtcatg aggcgaggaa
    14461 gagcttgctg gagagtagta ggatggccag gtggcaaagg cccatttgag gttgggaata
    14521 atcagtgggt aatgactccg atttgctccc atatcttttt tctctttagc aatactaagc
    14581 tgtatgagca caggcctgag ggtatatggt tcggtttgtc tgttgctcag agagtgacaa
    14641 gggacttgag gttattcgaa aggaagctgt tgtcatgatg gaatctaagc tgaatttaaa
    14701 aaaaaacaaa gtcaagccag tgggggcaga tgcagagagg gaaggccaag ttgggaggat
    14761 ctgtgaattg gtagtctgag tttcaaggag gcatttaaac aaatgagtgg gaaagggaga
    14821 gtgtggttgg atagtgggat atttgaattc atgatttgga agttgtgctg gttctggtga
    14881 taacactgag gatattgacc tttggaagtg tttggccaag ggggacccga aggaagggca
    14941 ctgtggccaa agggggcagt gttttgtcag aaatgtctca ccgtttagaa gcttgaagta
    15001 tatgcatggt gagccgcagg tgcattctgc aattgatcac tgggataaca tcaaacaatt
    15061 cctcctttac acatcccatc acttcaacac tattaagcag cacgtgtgac tggaggccca
    15121 gggcagccac aatccagaat gggtgatctt ctgttaggac atttatcttg gccataacta
    15181 gaactggagg tagtagggca gcacttggca gaactgagca tatttttaca tgtaattttg
    15241 tgatttattt ttgttcttgg ttgctaggaa gggggcttag aattaacatc cccagcatat
    15301 tatgaactga ggtttcttaa tggtgctttt ggagaacact tagctgagac acaacaataa
    15361 atgtttttct ggtcagtggg tgggggctgt gacaaggaga attggaatga tagttgtcat
    15421 ttttctgtct gcagaataag aggaggtcca gataatgagg tgatgaaaag gggtaggggg
    15481 aagaatagga agggtaggct ttgctattta aaagaatggg aaaaccctta aggagacttt
    15541 tctaaaagtg tggatatgaa aataaagcag gtatatgtaa aaaacacttt tcaatattgt
    15601 gatcagatag tgtgtaatgt cagtgacaca ccatagacat aaagctgacc cttcactgca
    15661 gcagacagat ttttatagtc aattagattc ctttggtaat gattccttcc ttgcctcctt
    15721 ctaggttaag cagctgcagg aggaaacgcc acctggtggt cctttaactg aagctttgcc
    15781 ccctgcccga aaggaaggtg atttgccccc actgtggtgg tatattgtga ccagaccccg
    15841 ggagcggccc atgtagaaag agagagacct catctttcat gcttgcaagt gaaatatgtt
    15901 acagaacatg cacttgccct aataaaaaat cagtgaaatg gtctctggta tgactgacgt
    15961 tcttctaaga attactgtga tgccaagtga ctgtgccact gtcattgttt tatcaatcgt
    16021 gcatgtaaag aaaatgacag gacatgtagt cagtcagctg tgttctctgc aataaatcac
    16081 tgatagcata tgttaccagt aaggaaaaac tgaggcagaa ccagattgag gcgaacaaac
    16141 tctcaagtca aaggcctaat tagctgtggt aactagaagg aataaacaaa gccaagaaca
    16201 gaaatactga ggatttaaaa ataacctttt tggttttcta aagatttcac taatggaact
    16261 tagtttttat tagaagaaca actctcccta agaagtacat agtcaacaaa taagcatgga
    16321 atttgtggag ttctgtcagg ggtggaggtt tgatggttgg ggttaaatgg tccaatctgg
    16381 gataaaactt tgtggcgctt attttagaag cagttttcca gctttgcttc tgctaccgta
    16441 gcttggattg tctgcggact caaaggtaag ggaataacag aactccttaa atgtttcaga
    16501 agctttgttt ttgtttttta aagataaagc agtttttaaa actttcaacc caaaacagct
    16561 gcttttagtt cttgatccct cattagctag aactgttttc aaatagtttc aggcaactaa
    16621 tgatcttata gccatcacaa aaatattttg tcacttttct acgtaagctc aatttgagtc
    16681 ccagtccatc ttctaacatt tgactaagac ctcaaactga attgtaacaa gacttgaaat
    16741 acaggacttt tttaatctag tggttcttta tttataacgt aggctgaagc aattgttaca
    16801 atgtaggagg gagacacatt acacaattgt tatttataca agtttagaac aaaaaactgc
    16861 acagggagag gtcaactctc agtacaaact agcgtttaaa ccaagcggcg actgagatgt
    16921 cctaaatgca cagcgacgga ttcgcgctat ttagaaagag agagcaatat ttcaagaatg
    16981 catgcgtcaa ttttacgcag actatctttc tagggttaat ctagctgcat caggatcata
    17041 tcgtcgggtc ttttttccgg ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg
    17101 aggaagaagc ccgtgccttt tcccgcgagg ttgaagcggc atggaaagag tttgccgagg
    17161 atgactgctg ctgcattgac gttgagcgaa aacgcacgtt taccatgatg attcgggaag
    17221 gtgtggccat gcacgccttt aacggtgaac tgttcgttca ggccacctgg gataccagtt
    17281 cgtcgcggct tttccggaca cagttccgga tggtcagccc gaagcgcatc agcaacccga
    17341 acaataccgg cgacagccgg aactgccgtg ccggtgtgca gattaatgac agcggtgcgg
    17401 cgctgggata ttacgtcagc gaggacgggt atcctggctg gatgccgcag aaatggacat
    17461 ggataccccg tgagttaccc ggcgggcgcg cttggcgtaa tcatggtcat agctgtttcc
    17521 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
    17581 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
    17641 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
    17701 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    17761 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    17821 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    17881 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    17941 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    18001 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    18061 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    18121 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    18181 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    18241 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    18301 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
    18361 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    18421 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    18481 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    18541 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    18601 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    18661 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    18721 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    18781 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    18841 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    18901 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    18961 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    19021 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    19081 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    19141 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    19201 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    19261 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    19321 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    19381 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    19441 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    19501 ggcgacacgg aaatgttgaa tactcat
//
