LOCUS       Exported               19526 bp ds-DNA     circular SYN 22-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19526)
  AUTHORS   The Weissman lab
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 6, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..19526
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    874..936
                     /label=5'ITR
     misc_feature    complement(1400..1993)
                     /label=WPRE
                     /label=WPRE; Woodchuck post-transcriptional
     primer_bind     complement(1893..1912)
                     /label=HKO083
     CDS             complement(2016..2615)
                     /label=Puro (-)
     promoter        complement(2628..3130)
                     /label=PGK (-)
     misc_feature    3137..16892
                     /label=NDUFB9-endogenous locus
     misc_feature    complement(5066..5149)
                     /label=ACTB 5UTR
     misc_feature    5066..5143
                     /label=exon 1
     misc_feature    complement(5144..5149)
                     /label=exon 2
     misc_feature    9216..9227
                     /label=NDUFB9
     misc_feature    9216..9227
                     /label=variant_bps
     misc_feature    9216..9227
                     /label=variation
     misc_feature    15856..15949
                     /label=3'UTR
     misc_feature    16980..17014
                     /label=3'ITR
     primer_bind     complement(17505..17521)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    17529..17545
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(17553..17583)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    17598..17619
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(17907..18495)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(18666..19526)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc agggtttatt acagggacag cagagatcca
     1381 gtttggttaa ttagctagcg atgcggggag gcggcccaaa gggagatccg actcgtctga
     1441 gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg
     1501 tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg
     1561 gcaacacagg cgagcagcca aggaaaggac gatgatttcc ccgacaacac cacggaattg
     1621 tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc
     1681 gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca
     1741 atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg
     1801 acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa
     1861 atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat
     1921 agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc
     1981 agaggttgat taccgataag cttgatatcg aattcttaac tggcaccggg cttgcgggtc
     2041 atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc
     2101 cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg
     2161 cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg
     2221 tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc
     2281 aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc
     2341 gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc
     2401 acggcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg
     2461 aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc
     2521 gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg
     2581 cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg tggtaccccc tggggagaga
     2641 ggtcggtgat tcggtcaacg agggagccga ctgccgacgt gcgctccgga ggcttgcaga
     2701 atgcggaaca ccgcgcgggc aggaacaggg cccacactac cgccccacac cccgcctccc
     2761 gcaccgcccc ttcccggccg ctgctctcgg cgcgccctgc tgagcagccg ctattggcca
     2821 cagcccatcg cggtcggcgc gctgccattg ctccctggcg ctgtccgtct gcgagggtac
     2881 tagtgagacg tgcggcttcc gtttgtcacg tccggcacgc cgcgaaccgc aaggaacctt
     2941 cccgacttag gggcggagca ggaagcgtcg ccggggggcc cacaagggta gcggcgaaga
     3001 tccgggtgac gctgcgaacg gacgtgaaga atgtgcgaga cccagggtcg gcgccgctgc
     3061 gtttcccgga accacgccca gagcagccgc gtccctgcgc aaacccaggg ctgccttgga
     3121 aaaggcgcaa acgcgtccca aacagtcatt gtactacgcc cctaggaaaa aaatataaat
     3181 accataccag tatctgcctt aagagtttga tctagtggga aaggcatact catatttaat
     3241 tataaataat gtggtataag ttttaaggag tgagtaggca gacaggcaac agggtagttg
     3301 gtaggcagag gaatattttg gccccaaact gccactctaa aaccttgtca gaggtaacaa
     3361 acagcatgca caagtgttta tagagcagcc cagggatggg ctagaaaagt gggcaggact
     3421 ggaccacaga agggctttgt atggtgtgta agcatttagg ctatcctgag gagcgtaaat
     3481 gaaagatttg aagcaaaaga aacatttagc tgatttgatt tttagataca ccaaaatgga
     3541 gtctacatgg aggatggatt taagacatta acttgaggca ggaatttggt tgtataacat
     3601 aaacacactt ttaaaaagtt atgtggcctt tctttacgga gtacttcatg gctctttatt
     3661 ccatctccct catcaattat atcaaaggtt aggatggaaa ggctaagaca cggcaatgtc
     3721 ttatttgaca acccctcccc aaacccctac tccaggcttt cccacagcaa tttcaaaacc
     3781 tttttcctgg cacttaacat attctcccct gcatttgctt ttgactgtac agtacatagg
     3841 tcttaggtct ccagtctgaa aactgcacca ctagatttta cagttccaca ggacttagca
     3901 caacgtcttt gaaaaaaaaa aatactgctt aaataactct cagatctaaa tgtataatcc
     3961 taatctcttg attgccagac cacattccca attgcccaga tatctctacc tggatgtcct
     4021 ataattatct caaacttaac ctaatccaaa tggacctggg tacctctctt caccgccaaa
     4081 ctagtcatgc ttccatttca tatttaagta cttttccaaa ccctgagtca tctttgttca
     4141 ctctctacta ccggccatga acactcagct gctaaatgct gcacacatct cttccatttc
     4201 tctctctttt ccattaccct agtttaagct cttgaaatgc ttccctacct ctccctattt
     4261 cccacaaaac ctacataata gctatcagga ttaccttgct aaagcagagg tgtgatcact
     4321 gtcatttccc accttcaggt agctggaaac cccacaaaac gaggatcatg cccatttaaa
     4381 tcccgcagca ttctccctct cgttctactg atgccgactg gatcattgta cttgccaagt
     4441 acagtttgtt tttctcccca ttacttgtgt tttctagttt ctccaccaga tggtgatgac
     4501 agtacaactg ggagctttgt gccctgtgtt cctaggccag tgccaggccc acttgaggcg
     4561 ctcactaact atgatatgaa cgaatgaatg cttgagtgaa ttggtcccca gaagcggtca
     4621 gcctctccgc caaggcagag gggaagggcc gggaggtgca cgtgggatga atggaaacat
     4681 agtgattggc ctttttggag ttttcttctt aaaaaactga atacattaca gatgttgaaa
     4741 acagcacaaa aatcataact gtacggcttg ttaattttta caaataaata caccctcgtg
     4801 aatccagaac ctagatcaag aaccagagca ttaccagcac ccagaaacct ccccgcgccc
     4861 tcctccactg agcggccctt tcaaccctta caatgccgga gggcgcaatt cctgggtcct
     4921 gtgaccttgg tgaccgacgc ccgggacaag tcagaaagac ccaagggcgt ggaaaacgct
     4981 cctcttaccg ataaacttga agcgactcat gactgcgcat ggaggacctc cccagcggaa
     5041 gcggaagtgg ccgccggcaa ctccgaccgc cgagaccgcg tccgccccgc gagcacagag
     5101 cctcgccttt gccgatccgc cgcccgtcca cacccgccgc cagctcacca tggcgttctt
     5161 ggcgtcggga ccctacctga cccatcagca aaaggtgttg cggctttata agcgggcgct
     5221 acgccacctc gagtcgtggt gcgtccagag gtaagggatg gggacccagg actcggggag
     5281 gtgaccctcg gggccccatg gaggtggaga ggcccctggg tacctggagg ttcaaggact
     5341 tcgggaacgg aattggaagg tggcctctcg cttcacagaa tcctatggta gacccgggtt
     5401 caaatccagg cttcgccact tatggccggg agaccttgta gagttgcttg tcgggtctgg
     5461 gcgtcagctt tttgtttagt aaaacggacg cacgaccagc gcagagggtc gcaggaaaat
     5521 taagtgaaat aaaggaaagt gcctaaaatt gtaattgtca tgggtccagc gctcgctcaa
     5581 tcgttttctt cacgatcttc tcctttccaa tattaacagc aataaactat gcattatttt
     5641 cttcagtctg caaaactcta ggagttagga attatcttaa gcttatttta cagataagaa
     5701 gatgagcaca gctggtaact tgcgcaaggt gatagagttt ctaagtgtct gagccagggt
     5761 taaaatttag gtctttctcc aaagcatcat cattctccca cccctcccat tagcttttct
     5821 ctttttagct tcatctagtc cttttcatta cctttacatc tttttcacct cttacataat
     5881 tctctttcga cctctatccg tctgagacta gtctgagaac atagtgttaa aatgtgtaga
     5941 ttttaatcac ctaaattcat tgcagggtgg gctaacaacc caagatgtgt gagtgcctcg
     6001 cctccactcc agtcttacga ctctccaaac gtagttttat aagaaggcct tctgggaaaa
     6061 aaactctatg gaaaatggag taggcctgga agaattacag agggtaccct cgaagcccag
     6121 attgcatata tgtgactatt agaaaatcac tgacatactt ctcttgattt ggcaggaaga
     6181 tggccaagta caatgtgatg ggattgttag tttcttcctt gagttttcct tatttattta
     6241 ttgatttgac agaatttgca attcagattc tatgctctat gctaaatata taaatgttag
     6301 taaatccatt tttgagcagc agcagctcac ggattagtgg ggaaaatgga catgtaaaca
     6361 aatcgatgat gaaggaagtt gagccatcag ggattcaggt atcccaacac aaatggtaaa
     6421 tgtttgaggt gatgaatatc caaattaccc tgatttgatc attatacatt atatggatgt
     6481 attaaaatat cacatgtacc ccataaatag gtacaataat tatgtgtcaa aaaaagttaa
     6541 acatgaaaaa aagaattcaa gcaatactga actatagctg ggaatactaa agtccatagt
     6601 tctagaggaa aatgatataa actagctact atgacagtta tggctcagca ttttttagat
     6661 ctgacattct ttctcaatcc ttgccttaat ttcttcctcc atttgccctc attgtgctgt
     6721 tctctgtggt tccatcctag gcatactgtg ttgttgtatg tactcgggca gcctcattta
     6781 gtgctgtcac cacctgcgct catttgtctc tagagctgta gttcgaattg cttgttagat
     6841 cacgactgtt ggctgggcat gatggctcac gcctataatc ccagtacttt gggaggctga
     6901 ggtgagtgga tcacctgagg ccaggagttc aacagcctgg cctacatgtg aaaccgcgtc
     6961 tctactaaaa atacaaaaat tagccaggcg tggtggtggg tgcctgtaat cccagctact
     7021 catgaggctg aggcagaaga accgcttgaa cctgggaggc agaggctgca gtgagccgag
     7081 atagccccat tgcactccag cctgggcgac aagagtgaaa ctttgtctca aaaaataata
     7141 ataaaaaaaa aattgaaaag atcaccaacc gttctctcaa tctccattta tctaaattct
     7201 ttgaaatcaa acttgttttc ctgtcactcc ccattctgac tcttttccca aattccctgc
     7261 ctccgctaat ggtattatta gtaattcagt tactgagctt taagtctcag aattgttctc
     7321 aagtatttgc tccttattcc tttgccaata acacacatac tcatacacca tgtataatta
     7381 gacactggtt tttataaatt ctctttccag tatgatatta gtaactgaca ttatttaaca
     7441 cttgggatgt accaggcact ttttaaagtg tgtttatctt attgactgtt tatgacaacc
     7501 ttaagatata cacactgtta ttgccactgt tttacagagg aggaacccgt ggcctagaag
     7561 actttttttg tttgtttttt tgttagacgg agtttcgctt atgttgccca ggctggagtg
     7621 cagtggcgcg atcttggctc accgcaagtt ccgtctcccg ggttcaagca attctcctgc
     7681 ctcagtctcc cgagtagctg ggattacagg catgcgccac cacgcctggc taatttttgt
     7741 atttttagta gagacggggt tttgccatgt tggccaggct ggtcttgaac tcctgacctc
     7801 aggtgacctc cacctcagcc tcccaaagtg ctgggattac aggcgtgagc caccgtgcct
     7861 ggcctttttg ttgttgttgt tgttgttttt ttttgagatg aagtctcgct cttgtccccc
     7921 aggctggagt gcaaatggca caatctcgac tcactgcaac ctctgcctcc cgggttcaag
     7981 ctattctcct gccttggccc ccagagtagc tgggattaca ggtgcctgcc accacgcccg
     8041 gctagttttt gtatttttag ctgagacggg atttcaccat gttggccagg ctggtgtaga
     8101 actcctcacc tcagatgatc cccctgcctt ggcctcccaa agtgctggga ttacaggcgt
     8161 gagccaccat gcccggcctc atttttgtat ttttagtaga gacggggttt ctccatgttg
     8221 atcaggcttg tcttgaactc cagacctcag gtgatccaca cgccttggcc tcccaacgtg
     8281 ctgggattac aggtgtgagc caccgcacct gaccagaaga cagtaactta ccccaagtta
     8341 cacagctagt agtggcagag ctaagattca ggcccataca gtctgactgg agagcctatt
     8401 ctcataactt ggaagtctca atgtctcccc tgtctcctgt caccatcaca gctccgtact
     8461 ttcaggccct cgtcagctgt gtcctagatt attgtcaaaa cctcctaact gatctttgcc
     8521 cagtttcttt agaacttgac tttaacttgc ctaagtttat gcagctagtg tcagagtcag
     8581 gatacaaacc tagctctgta tgagctaagc aatactgttt ctgccaagta acacctttct
     8641 tattcatctt tgtatctcca gaatttagca tagtgactag cacaagatag aaggaaatgg
     8701 aagttgtcat tcaatgaatg ttgaatgatc ctgcagaggt tatagctttt ctcttgcgaa
     8761 tgctcttttc tttttttttt tttttttttt tgtttaaatc ctcattccat tgttggatgt
     8821 aagaggagaa aaatggttag ggtatatatt taatacagta tttttttgct gctgctgttt
     8881 ggtgggctgt agaggaagca gccatgatga tacggctgct ccattgggag ggttggggta
     8941 ggtccactgc ccatccagaa ggtatcagaa gtgcagcctc ctgtcagaca gcaacactga
     9001 ccacgtgagt aggtaacatt tctaatcttc aaaatcattt tggtttaaga gacaaatacc
     9061 gatactttgc ttgtttgatg agagcccggt ttgaagaaca taagaatgaa aaggatatgg
     9121 cgaaggccac ccagctgctg aaggaggccg aggaagaatt ctggtaccgt cagcatccac
     9181 agccatacat cttccctgac tctcctgggg gcaccagcta tgagcggtac gattgctaca
     9241 aggtaggtga gaattatgat gactgccttc tgagaaatag actttgtttc ttcagtccca
     9301 cacctcagcc tcagaaatca cttgcccttc aaggggtagc taggagactt atttcctgac
     9361 agttgtcaga tgcaggccta cctcatttta ttgcattttg ttttattgtg cttggcagat
     9421 attgtatttt cacagattga aagtttgtgg caaccccgtg ctgagcaagt ctgtgggcac
     9481 catttttcca acagcatgtg ctcactccct gtctctgttt ctcagtttgg caattcttgc
     9541 aatatttcaa actttttttt attattactc tatctacaca cagttgtggt gaactgtgat
     9601 cagtcacctt tgatgttact attgtcattg ttttggggca caaaaaaacc atgcccacat
     9661 aagacagcag acttaatcga taaatgtgcg tgtgctgact gtttcaccga ccaatagttc
     9721 ctccgcctct atccctctcc tcagggttcc ctattcccta agacgcagca acagtgaaat
     9781 taggacaatt aacgaccata ccatagcctc taagtgttca agtgaaagga agagttacat
     9841 gtctctcact ttaaatccgt tacacgtctc ttcctttaaa tcaaaagcta gaaatgatta
     9901 agcttagtga ggaaggcatg ttgaaagcca aggtgggtag aaagcaaggc cttttccgct
     9961 ggttagccaa gttgtgaatg caaaggaaat taaaggtttt tttaaggaaa ttaaaagtgc
    10021 tgctccacta aacacacaaa tgataagtga aactgtctta ttgctgatat ggagaaagtt
    10081 ttagtggttg ggacaaaaga tcaaaccagc cacgacattc ccttaagcca aagcctaatc
    10141 caaagcaagt ctttaactct tcatttctat gaaggctgag agagatgagg aagctgcaga
    10201 aaaaaagttg gaagctaata gaggttggtc catgaggttt aaggaaaaaa gccatctctg
    10261 taacacagat gtgcaaggtg aagcaacaag tgctgatgga aaagctgcag caaattatta
    10321 cacaaaatct ggctaagata gttgatgaag gtggctatac ttaacaacag attttcagtg
    10381 gagatgaagt agcctttttt tggaataaga taccatgtag aactttcata ccttgagaga
    10441 aatcaatgct tggcttcaaa acttcaagga taggctgact ctcttgttag ggactaatgc
    10501 agttggtggt tttaagttga agccattgtt catttgccat tctgaaaatc ctgaggccct
    10561 taagaattat gctaaaccta ctctgcctgt gccctagaaa tggaataaca aagcctgaat
    10621 tacagcaaat ctgtatacaa catggtttac tgaatctttt aagcccattg ttgagacctg
    10681 ctaccatgtc tgctaacaca acatccattc tgcagcccat ggatcaagga gtaatttcca
    10741 cttgcaagtt ttattattta agaaatatat tttgcatagt tatagttgcc atagatagtg
    10801 attcctctga tggatttggg caaagtaaaa ttttctggaa agaattcacc attctaaatg
    10861 ccattgagga tatttatgat tcatgggaag agatcaaaat atcagcttta acggggttgg
    10921 aagaagttga ttccaaccct tgtgggtgat tttgaagggc tcaagacttc agcagaagaa
    10981 gtaactgcag aagttgtgga aatagcaaga gagccagaat agaagctgat cctgagaata
    11041 tgactgaatt gctgtaatct catgataaat cttgagcaga tgaagagttg cttcttatgg
    11101 atgaatacga aagtggtttc ttgagatgga gtctcctggt gaagtttttg tgaacattga
    11161 tgataacaaa ggacttcgca tagcacatca acttagttga ttaagcagta gcagggtttg
    11221 agaggattga ctccaatttt gcaagaagtt cttctgtggg taaaatgcta tcaaatggca
    11281 tcacatgcta tagaagtgtt tggtgtaagg aagagtcagt tgatgtcgca aacctcgtta
    11341 ttttaggaaa ttgccaaagc cactccagcc tttggcaatc atcaccctga ttagtcacca
    11401 gccatcaaca ttgatataat actctccacc agtaaaaaga ttggttttct gatggctcag
    11461 atgattgttg gcagttttta tcaataacgt atttcttttg tttttgtttg ggagatggtc
    11521 tccctctgtt gcccaggctg gagtgcaata gcatgatcat agctcactgc agcctggatc
    11581 ccctgggctc aagcaatcct cctgtcctag ccaaccaagt agctaagaaa acagaggcac
    11641 aacaccacac ctagctaatt tattttattt tttgtagaga tggaatcttg ctatgttgtc
    11701 caggctggtc tggaactcct accctcatag aatctccctg cctcagcctt caaagggctg
    11761 ggactaaagg caggagccac ctcacccaac caataatgta tttttaaatt atgtcttttt
    11821 tttggtggag ggggacagag ttttgcccag gctggagtgc aatggcacaa tctcggctca
    11881 ccgcaacctc cacctcccag gttcaagcga ttctcctacc tcagtctccc aagtagctgg
    11941 gattacaggc atgtaccact atgcctggct agttttgtat ttttagtaga gacagcgttt
    12001 ctccatgttg gtcaggctgg tctcgaactc ctgacctcag gtgatccgcc tgcctcagct
    12061 tcccaaagtg ctgggattac aggcgtgagc cactgcacct ggcctaaatt atgtacattt
    12121 tttatgtata ttatgtgagg tttgccacat caattgactt ttccttttga tagcatttta
    12181 cccacagaag aacttctttc aaaatgggaa tcaatcctct caaaccctgc cactgcttta
    12241 tcaactaagt tgatgtactg ttcaaagtcc tttgttatca gttaatcaat attcacaaaa
    12301 tcttcaccag gaggactcca tctcaacaga catcataatg ctgttggaca ctttatagta
    12361 tagtataaac aaaactttta tatgcactgg gaaaccaaaa acattttgtg acttgcttta
    12421 ttgtgatatt tgctatattg cagcggtctg gaaccaaacc cccagtatct ctaaggtgtg
    12481 cctgtactgt ttcttaggtg tcagttttct ctctctgaga cgtcattaag acttttcttt
    12541 ctacattcag atttaaggtc gtacagtata ctttttgcta taacaaagtt cattttggac
    12601 tttaaagtta accaacttgg attggtgctt aatatttctg agatactcta attaagagtt
    12661 ccaagtaaga taagactact ctggatattt agagaaggca ggtaggggag gaatgaagcc
    12721 agttctcctt ggactgatga tggtgctatt tttcagcaca gtctttgact ctaaggtgtt
    12781 ttcctttatc tttgcttcct acttatcctc tgctgttcct tgcttagagt tagaaacagt
    12841 ctggtagttg cagtggttat aaaaatatat cttgatcttt gtcttacatc tcctgagcag
    12901 gccctgtcag ggataggtaa atgtgtcctg tggattgata ccatgatttc ctctcctgac
    12961 aggtcccaga atggtgctta gatgactggc atccttctga gaaggcaatg tatcctgatt
    13021 actttgccaa gagagaacag tggaagaaac tgcggaggga aagctgggaa cgagaggtgc
    13081 gttctacttg tacttcgtta ttccttagct atgtagatgg agtgaagttt tgctccccac
    13141 aagcagaggt ccagattcca gccatgacag ttactctggg cccttgcttc tcagatatac
    13201 tttagtatct aagtgtatca atttaacatt tataatcacc ttacaagtaa gttaaagtgc
    13261 attccctggt cgttttggct ttgtctttga ttttgcttca gatctccttc tctttagtcc
    13321 tttgtctgac ttttttgcct tcttgctata ctttccctca acctgcctct ccactatttt
    13381 cttaggcatt tttcagtcat gttaggatgg tcagttatat ttttaaaagc tcaatcttgc
    13441 tgtagtgtga agaatatatt ggagagggga agaggagaag cagagagacc cttctaggat
    13501 tattgcagaa gtttagtcaa aaggttgtca tgactaacac tagggtggat gaagtggata
    13561 tggaaggaaa ttgactcagg acatcatttg gagggatgtg acgactgact ggatgttgag
    13621 ggtgggtgag ggatggggag gaatcaagaa tggtttctgg ggcagggtgc ggtggctcac
    13681 acctataatc ccagcacttt gggaggccaa agtgggtgat agcttgaggt caggagttca
    13741 tgacgaccag cctgactaac atggtgaaac cccatctcta ctaaaagtac aaataattag
    13801 ctgggcgtgg gggctgtgca cctgtaatcc cagctacttg ggaggctaag gcaggagaat
    13861 tgcttgaatc ccaggaggtg gaggttgcaa tgagctgaca tcgcgccact gcattccagc
    13921 ctgggcgaca gagcaagact ccatcccaaa agaaaaaaaa aagagagaat ggtttctggg
    13981 ttttaagctt taacatctgg agtttggtga tgtttactga gatagggaag attggggaag
    14041 ggggcacagg tgtcagagca gcaatcgaga attcattttg gaagtgtgaa gttttgagaa
    14101 tcctgtgaga catcaagtgc agatgtcaga agccaacttc agatgtggaa atctggaact
    14161 cccaggagag atttggccta gaaacagaaa cgtgggagtt ttcagtttta aaagccatgg
    14221 agagatgtat ggaatcctta ggagagagta tagagaaaaa agaaaaggaa aaaggattct
    14281 ttgtgcatta taattgggca aaagattgta tataaataca tttgggttag aagttttgaa
    14341 gatgacagac ttgtgtgata gcttcctttt tttagtgaaa taagaggaaa agcccagaat
    14401 gagtgggtgc agcatatttg gagaatgtgg acaagatgtg atagtcatga ggcgaggaag
    14461 agcttgctgg agagtagtag gatggccagg tggcaaaggc ccatttgagg ttgggaataa
    14521 tcagtgggta atgactccga tttgctccca tatctttttt ctctttagca atactaagct
    14581 gtatgagcac aggcctgagg gtatatggtt cggtttgtct gttgctcaga gagtgacaag
    14641 ggacttgagg ttattcgaaa ggaagctgtt gtcatgatgg aatctaagct gaatttaaaa
    14701 aaaaacaaag tcaagccagt gggggcagat gcagagaggg aaggccaagt tgggaggatc
    14761 tgtgaattgg tagtctgagt ttcaaggagg catttaaaca aatgagtggg aaagggagag
    14821 tgtggttgga tagtgggata tttgaattca tgatttggaa gttgtgctgg ttctggtgat
    14881 aacactgagg atattgacct ttggaagtgt ttggccaagg gggacccgaa ggaagggcac
    14941 tgtggccaaa gggggcagtg ttttgtcaga aatgtctcac cgtttagaag cttgaagtat
    15001 atgcatggtg agccgcaggt gcattctgca attgatcact gggataacat caaacaattc
    15061 ctcctttaca catcccatca cttcaacact attaagcagc acgtgtgact ggaggcccag
    15121 ggcagccaca atccagaatg ggtgatcttc tgttaggaca tttatcttgg ccataactag
    15181 aactggaggt agtagggcag cacttggcag aactgagcat atttttacat gtaattttgt
    15241 gatttatttt tgttcttggt tgctaggaag ggggcttaga attaacatcc ccagcatatt
    15301 atgaactgag gtttcttaat ggtgcttttg gagaacactt agctgagaca caacaataaa
    15361 tgtttttctg gtcagtgggt gggggctgtg acaaggagaa ttggaatgat agttgtcatt
    15421 tttctgtctg cagaataaga ggaggtccag ataatgaggt gatgaaaagg ggtaggggga
    15481 agaataggaa gggtaggctt tgctatttaa aagaatggga aaacccttaa ggagactttt
    15541 ctaaaagtgt ggatatgaaa ataaagcagg tatatgtaaa aaacactttt caatattgtg
    15601 atcagatagt gtgtaatgtc agtgacacac catagacata aagctgaccc ttcactgcag
    15661 cagacagatt tttatagtca attagattcc tttggtaatg attccttcct tgcctccttc
    15721 taggttaagc agctgcagga ggaaacgcca cctggtggtc ctttaactga agctttgccc
    15781 cctgcccgaa aggaaggtga tttgccccca ctgtggtggt atattgtgac cagaccccgg
    15841 gagcggccca tgtagaaaga gagagacctc atctttcatg cttgcaagtg aaatatgtta
    15901 cagaacatgc acttgcccta ataaaaaatc agtgaaatgg tctctggtat gactgacgtt
    15961 cttctaagaa ttactgtgat gccaagtgac tgtgccactg tcattgtttt atcaatcgtg
    16021 catgtaaaga aaatgacagg acatgtagtc agtcagctgt gttctctgca ataaatcact
    16081 gatagcatat gttaccagta aggaaaaact gaggcagaac cagattgagg cgaacaaact
    16141 ctcaagtcaa aggcctaatt agctgtggta actagaagga ataaacaaag ccaagaacag
    16201 aaatactgag gatttaaaaa taaccttttt ggttttctaa agatttcact aatggaactt
    16261 agtttttatt agaagaacaa ctctccctaa gaagtacata gtcaacaaat aagcatggaa
    16321 tttgtggagt tctgtcaggg gtggaggttt gatggttggg gttaaatggt ccaatctggg
    16381 ataaaacttt gtggcgctta ttttagaagc agttttccag ctttgcttct gctaccgtag
    16441 cttggattgt ctgcggactc aaaggtaagg gaataacaga actccttaaa tgtttcagaa
    16501 gctttgtttt tgttttttaa agataaagca gtttttaaaa ctttcaaccc aaaacagctg
    16561 cttttagttc ttgatccctc attagctaga actgttttca aatagtttca ggcaactaat
    16621 gatcttatag ccatcacaaa aatattttgt cacttttcta cgtaagctca atttgagtcc
    16681 cagtccatct tctaacattt gactaagacc tcaaactgaa ttgtaacaag acttgaaata
    16741 caggactttt ttaatctagt ggttctttat ttataacgta ggctgaagca attgttacaa
    16801 tgtaggaggg agacacatta cacaattgtt atttatacaa gtttagaaca aaaaactgca
    16861 cagggagagg tcaactctca gtacaaacta gcgtttaaac caagcggcga ctgagatgtc
    16921 ctaaatgcac agcgacggat tcgcgctatt tagaaagaga gagcaatatt tcaagaatgc
    16981 atgcgtcaat tttacgcaga ctatctttct agggttaatc tagctgcatc aggatcatat
    17041 cgtcgggtct tttttccggc tcagtcatcg cccaagctgg cgctatctgg gcatcgggga
    17101 ggaagaagcc cgtgcctttt cccgcgaggt tgaagcggca tggaaagagt ttgccgagga
    17161 tgactgctgc tgcattgacg ttgagcgaaa acgcacgttt accatgatga ttcgggaagg
    17221 tgtggccatg cacgccttta acggtgaact gttcgttcag gccacctggg ataccagttc
    17281 gtcgcggctt ttccggacac agttccggat ggtcagcccg aagcgcatca gcaacccgaa
    17341 caataccggc gacagccgga actgccgtgc cggtgtgcag attaatgaca gcggtgcggc
    17401 gctgggatat tacgtcagcg aggacgggta tcctggctgg atgccgcaga aatggacatg
    17461 gataccccgt gagttacccg gcgggcgcgc ttggcgtaat catggtcata gctgtttcct
    17521 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    17581 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    17641 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    17701 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    17761 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    17821 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    17881 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    17941 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    18001 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    18061 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    18121 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    18181 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    18241 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    18301 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
    18361 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    18421 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    18481 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    18541 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    18601 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    18661 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    18721 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    18781 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    18841 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    18901 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    18961 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    19021 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    19081 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    19141 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    19201 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    19261 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    19321 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    19381 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    19441 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    19501 gcgacacgga aatgttgaat actcat
//
