LOCUS       Exported               20373 bp ds-DNA     circular SYN 22-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20373)
  AUTHORS   The Weissman lab
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 6, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..20373
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    874..936
                     /label=5'ITR
     misc_feature    complement(1400..1993)
                     /label=WPRE
                     /label=WPRE; Woodchuck post-transcriptional
     primer_bind     complement(1893..1912)
                     /label=HKO083
     CDS             complement(2016..2615)
                     /label=Puro (-)
     misc_feature    complement(2616..2678)
                     /label=T2A (-)
     misc_feature    2679..3386
                     /label=mCherry (-)
     promoter        complement(3396..3556)
                     /label=PGK (-)
     promoter        complement(3557..3898)
                     /label=PGK (-)
     misc_feature    3905..17739
                     /label=NDUFB9-endogenous locus
     misc_feature    5834..5854
                     /label=additional 5'UTR
     misc_feature    5855..5918
                     /label=5'UTR
     misc_feature    9985..9996
                     /label=NDUFB9
     misc_feature    9985..9996
                     /label=variant_bps
     misc_feature    9985..9996
                     /label=variation
     CDS             16622..16651
                     /codon_start=1
                     /label=(GS)5 linker
                     /translation="GSGSGSGSGS"
     CDS             16652..16699
                     /codon_start=1
                     /label=mNeon11
                     /translation="TELNFKEWQKAFTDMM"
     misc_feature    16703..16796
                     /label=3'UTR
     misc_feature    17827..17861
                     /label=3'ITR
     primer_bind     complement(18352..18368)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    18376..18392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(18400..18430)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    18445..18466
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(18754..19342)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(19513..20373)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc agggtttatt acagggacag cagagatcca
     1381 gtttggttaa ttagctagcg atgcggggag gcggcccaaa gggagatccg actcgtctga
     1441 gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg
     1501 tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg
     1561 gcaacacagg cgagcagcca aggaaaggac gatgatttcc ccgacaacac cacggaattg
     1621 tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc
     1681 gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca
     1741 atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg
     1801 acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa
     1861 atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat
     1921 agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc
     1981 agaggttgat taccgataag cttgatatcg aattcttaac tggcaccggg cttgcgggtc
     2041 atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc
     2101 cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg
     2161 cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg
     2221 tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc
     2281 aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc
     2341 gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc
     2401 acggcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg
     2461 aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc
     2521 gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg
     2581 cgggtggcga ggcgcaccgt gggcttgtac tcggttgggc caggattctc ctcgacgtca
     2641 ccgcatgtta gcagacttcc tctgccctct ccactgccct tgtacagctc gtccatgccg
     2701 ccggtggagt ggcggccctc ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg
     2761 tgggaggtga tgtccaactt gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc
     2821 ttggccttgt aggtggtctt gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc
     2881 ctctgcttga tctcgccctt cagggcgccg tcctcggggt acatccgctc ggaggaggcc
     2941 tcccagccca tggtcttctt ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc
     3001 agcttcacct tgtagatgaa ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc
     3061 acgccgccgt cctcgaagtt catcacgcgc tcccacttga agccctcggg gaaggacagc
     3121 ttcaagtagt cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac
     3181 tgaggggaca ggatgtccca ggcgaagggc agggggccac ccttggtcac cttcagcttg
     3241 gcggtctggg tgccctcgta ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg
     3301 ccgttcacgg agccctccat gtgcaccttg aagcgcatga actccttgat gatggccatg
     3361 ttatcctcct cgcccttgct caccatggtg gtaccccctg gggagagagg tcggtgattc
     3421 ggtcaacgag ggagccgact gccgacgtgc gctccggagg cttgcagaat gcggaacacc
     3481 gcgcgggcag gaacagggcc cacactaccg ccccacaccc cgcctcccgc accgcccctt
     3541 cccggccgct gctctcggcg cgccctgctg agcagccgct attggccaca gcccatcgcg
     3601 gtcggcgcgc tgccattgct ccctggcgct gtccgtctgc gagggtacta gtgagacgtg
     3661 cggcttccgt ttgtcacgtc cggcacgccg cgaaccgcaa ggaaccttcc cgacttaggg
     3721 gcggagcagg aagcgtcgcc ggggggccca caagggtagc ggcgaagatc cgggtgacgc
     3781 tgcgaacgga cgtgaagaat gtgcgagacc cagggtcggc gccgctgcgt ttcccggaac
     3841 cacgcccaga gcagccgcgt ccctgcgcaa acccagggct gccttggaaa aggcgcaaat
     3901 cgatcccaaa cagtcattgt actacgcccc taggaaaaaa atataaatac cataccagta
     3961 tctgccttaa gagtttgatc tagtgggaaa ggcatactca tatttaatta taaataatgt
     4021 ggtataagtt ttaaggagtg agtaggcaga caggcaacag ggtagttggt aggcagagga
     4081 atattttggc cccaaactgc cactctaaaa ccttgtcaga ggtaacaaac agcatgcaca
     4141 agtgtttata gagcagccca gggatgggct agaaaagtgg gcaggactgg accacagaag
     4201 ggctttgtat ggtgtgtaag catttaggct atcctgagga gcgtaaatga aagatttgaa
     4261 gcaaaagaaa catttagctg atttgatttt tagatacacc aaaatggagt ctacatggag
     4321 gatggattta agacattaac ttgaggcagg aatttggttg tataacataa acacactttt
     4381 aaaaagttat gtggcctttc tttacggagt acttcatggc tctttattcc atctccctca
     4441 tcaattatat caaaggttag gatggaaagg ctaagacacg gcaatgtctt atttgacaac
     4501 ccctccccaa acccctactc caggctttcc cacagcaatt tcaaaacctt tttcctggca
     4561 cttaacatat tctcccctgc atttgctttt gactgtacag tacataggtc ttaggtctcc
     4621 agtctgaaaa ctgcaccact agattttaca gttccacagg acttagcaca acgtctttga
     4681 aaaaaaaaaa tactgcttaa ataactctca gatctaaatg tataatccta atctcttgat
     4741 tgccagacca cattcccaat tgcccagata tctctacctg gatgtcctat aattatctca
     4801 aacttaacct aatccaaatg gacctgggta cctctcttca ccgccaaact agtcatgctt
     4861 ccatttcata tttaagtact tttccaaacc ctgagtcatc tttgttcact ctctactacc
     4921 ggccatgaac actcagctgc taaatgctgc acacatctct tccatttctc tctcttttcc
     4981 attaccctag tttaagctct tgaaatgctt ccctacctct ccctatttcc cacaaaacct
     5041 acataatagc tatcaggatt accttgctaa agcagaggtg tgatcactgt catttcccac
     5101 cttcaggtag ctggaaaccc cacaaaacga ggatcatgcc catttaaatc ccgcagcatt
     5161 ctccctctcg ttctactgat gccgactgga tcattgtact tgccaagtac agtttgtttt
     5221 tctccccatt acttgtgttt tctagtttct ccaccagatg gtgatgacag tacaactggg
     5281 agctttgtgc cctgtgttcc taggccagtg ccaggcccac ttgaggcgct cactaactat
     5341 gatatgaacg aatgaatgct tgagtgaatt ggtccccaga agcggtcagc ctctccgcca
     5401 aggcagaggg gaagggccgg gaggtgcacg tgggatgaat ggaaacatag tgattggcct
     5461 ttttggagtt ttcttcttaa aaaactgaat acattacaga tgttgaaaac agcacaaaaa
     5521 tcataactgt acggcttgtt aatttttaca aataaataca ccctcgtgaa tccagaacct
     5581 agatcaagaa ccagagcatt accagcaccc agaaacctcc ccgcgccctc ctccactgag
     5641 cggccctttc aacccttaca atgccggagg gcgcaattcc tgggtcctgt gaccttggtg
     5701 accgacgccc gggacaagtc agaaagaccc aagggcgtgg aaaacgctcc tcttaccgat
     5761 aaacttgaag cgactcatga ctgcgcatgg aggacctccc cagcggaagc ggaagtggcc
     5821 gccggcaact ccgcccttcc ggctggcccc gctcagtcac ccgcagcagg cgtgcagttt
     5881 cccggctctc cgcgcggccg gggaaggtca gcgccgtaat ggcgttcttg gcgtcgggac
     5941 cctacctgac ccatcagcaa aaggtgttgc ggctttataa gcgggcgcta cgccacctcg
     6001 agtcgtggtg cgtccagagg taagggatgg ggacccagga ctcggggagg tgaccctcgg
     6061 ggccccatgg aggtggagag gcccctgggt acctggaggt tcaaggactt cgggaacgga
     6121 attggaaggt ggcctctcgc ttcacagaat cctatggtag acccgggttc aaatccaggc
     6181 ttcgccactt atggccggga gaccttgtag agttgcttgt cgggtctggg cgtcagcttt
     6241 ttgtttagta aaacggacgc acgaccagcg cagagggtcg caggaaaatt aagtgaaata
     6301 aaggaaagtg cctaaaattg taattgtcat gggtccagcg ctcgctcaat cgttttcttc
     6361 acgatcttct cctttccaat attaacagca ataaactatg cattattttc ttcagtctgc
     6421 aaaactctag gagttaggaa ttatcttaag cttattttac agataagaag atgagcacag
     6481 ctggtaactt gcgcaaggtg atagagtttc taagtgtctg agccagggtt aaaatttagg
     6541 tctttctcca aagcatcatc attctcccac ccctcccatt agcttttctc tttttagctt
     6601 catctagtcc ttttcattac ctttacatct ttttcacctc ttacataatt ctctttcgac
     6661 ctctatccgt ctgagactag tctgagaaca tagtgttaaa atgtgtagat tttaatcacc
     6721 taaattcatt gcagggtggg ctaacaaccc aagatgtgtg agtgcctcgc ctccactcca
     6781 gtcttacgac tctccaaacg tagttttata agaaggcctt ctgggaaaaa aactctatgg
     6841 aaaatggagt aggcctggaa gaattacaga gggtaccctc gaagcccaga ttgcatatat
     6901 gtgactatta gaaaatcact gacatacttc tcttgatttg gcaggaagat ggccaagtac
     6961 aatgtgatgg gattgttagt ttcttccttg agttttcctt atttatttat tgatttgaca
     7021 gaatttgcaa ttcagattct atgctctatg ctaaatatat aaatgttagt aaatccattt
     7081 ttgagcagca gcagctcacg gattagtggg gaaaatggac atgtaaacaa atcgatgatg
     7141 aaggaagttg agccatcagg gattcaggta tcccaacaca aatggtaaat gtttgaggtg
     7201 atgaatatcc aaattaccct gatttgatca ttatacatta tatggatgta ttaaaatatc
     7261 acatgtaccc cataaatagg tacaataatt atgtgtcaaa aaaagttaaa catgaaaaaa
     7321 agaattcaag caatactgaa ctatagctgg gaatactaaa gtccatagtt ctagaggaaa
     7381 atgatataaa ctagctacta tgacagttat ggctcagcat tttttagatc tgacattctt
     7441 tctcaatcct tgccttaatt tcttcctcca tttgccctca ttgtgctgtt ctctgtggtt
     7501 ccatcctagg catactgtgt tgttgtatgt actcgggcag cctcatttag tgctgtcacc
     7561 acctgcgctc atttgtctct agagctgtag ttcgaattgc ttgttagatc acgactgttg
     7621 gctgggcatg atggctcacg cctataatcc cagtactttg ggaggctgag gtgagtggat
     7681 cacctgaggc caggagttca acagcctggc ctacatgtga aaccgcgtct ctactaaaaa
     7741 tacaaaaatt agccaggcgt ggtggtgggt gcctgtaatc ccagctactc atgaggctga
     7801 ggcagaagaa ccgcttgaac ctgggaggca gaggctgcag tgagccgaga tagccccatt
     7861 gcactccagc ctgggcgaca agagtgaaac tttgtctcaa aaaataataa taaaaaaaaa
     7921 attgaaaaga tcaccaaccg ttctctcaat ctccatttat ctaaattctt tgaaatcaaa
     7981 cttgttttcc tgtcactccc cattctgact cttttcccaa attccctgcc tccgctaatg
     8041 gtattattag taattcagtt actgagcttt aagtctcaga attgttctca agtatttgct
     8101 ccttattcct ttgccaataa cacacatact catacaccat gtataattag acactggttt
     8161 ttataaattc tctttccagt atgatattag taactgacat tatttaacac ttgggatgta
     8221 ccaggcactt tttaaagtgt gtttatctta ttgactgttt atgacaacct taagatatac
     8281 acactgttat tgccactgtt ttacagagga ggaacccgtg gcctagaaga ctttttttgt
     8341 ttgttttttt gttagacgga gtttcgctta tgttgcccag gctggagtgc agtggcgcga
     8401 tcttggctca ccgcaagttc cgtctcccgg gttcaagcaa ttctcctgcc tcagtctccc
     8461 gagtagctgg gattacaggc atgcgccacc acgcctggct aatttttgta tttttagtag
     8521 agacggggtt ttgccatgtt ggccaggctg gtcttgaact cctgacctca ggtgacctcc
     8581 acctcagcct cccaaagtgc tgggattaca ggcgtgagcc accgtgcctg gcctttttgt
     8641 tgttgttgtt gttgtttttt tttgagatga agtctcgctc ttgtccccca ggctggagtg
     8701 caaatggcac aatctcgact cactgcaacc tctgcctccc gggttcaagc tattctcctg
     8761 ccttggcccc cagagtagct gggattacag gtgcctgcca ccacgcccgg ctagtttttg
     8821 tatttttagc tgagacggga tttcaccatg ttggccaggc tggtgtagaa ctcctcacct
     8881 cagatgatcc ccctgccttg gcctcccaaa gtgctgggat tacaggcgtg agccaccatg
     8941 cccggcctca tttttgtatt tttagtagag acggggtttc tccatgttga tcaggcttgt
     9001 cttgaactcc agacctcagg tgatccacac gccttggcct cccaacgtgc tgggattaca
     9061 ggtgtgagcc accgcacctg accagaagac agtaacttac cccaagttac acagctagta
     9121 gtggcagagc taagattcag gcccatacag tctgactgga gagcctattc tcataacttg
     9181 gaagtctcaa tgtctcccct gtctcctgtc accatcacag ctccgtactt tcaggccctc
     9241 gtcagctgtg tcctagatta ttgtcaaaac ctcctaactg atctttgccc agtttcttta
     9301 gaacttgact ttaacttgcc taagtttatg cagctagtgt cagagtcagg atacaaacct
     9361 agctctgtat gagctaagca atactgtttc tgccaagtaa cacctttctt attcatcttt
     9421 gtatctccag aatttagcat agtgactagc acaagataga aggaaatgga agttgtcatt
     9481 caatgaatgt tgaatgatcc tgcagaggtt atagcttttc tcttgcgaat gctcttttct
     9541 tttttttttt tttttttttt gtttaaatcc tcattccatt gttggatgta agaggagaaa
     9601 aatggttagg gtatatattt aatacagtat ttttttgctg ctgctgtttg gtgggctgta
     9661 gaggaagcag ccatgatgat acggctgctc cattgggagg gttggggtag gtccactgcc
     9721 catccagaag gtatcagaag tgcagcctcc tgtcagacag caacactgac cacgtgagta
     9781 ggtaacattt ctaatcttca aaatcatttt ggtttaagag acaaataccg atactttgct
     9841 tgtttgatga gagcccggtt tgaagaacat aagaatgaaa aggatatggc gaaggccacc
     9901 cagctgctga aggaggccga ggaagaattc tggtaccgtc agcatccaca gccatacatc
     9961 ttccctgact ctcctggggg caccagctat gagcggtacg attgctacaa ggtaggtgag
    10021 aattatgatg actgccttct gagaaataga ctttgtttct tcagtcccac acctcagcct
    10081 cagaaatcac ttgcccttca aggggtagct aggagactta tttcctgaca gttgtcagat
    10141 gcaggcctac ctcattttat tgcattttgt tttattgtgc ttggcagata ttgtattttc
    10201 acagattgaa agtttgtggc aaccccgtgc tgagcaagtc tgtgggcacc atttttccaa
    10261 cagcatgtgc tcactccctg tctctgtttc tcagtttggc aattcttgca atatttcaaa
    10321 ctttttttta ttattactct atctacacac agttgtggtg aactgtgatc agtcaccttt
    10381 gatgttacta ttgtcattgt tttggggcac aaaaaaacca tgcccacata agacagcaga
    10441 cttaatcgat aaatgtgcgt gtgctgactg tttcaccgac caatagttcc tccgcctcta
    10501 tccctctcct cagggttccc tattccctaa gacgcagcaa cagtgaaatt aggacaatta
    10561 acgaccatac catagcctct aagtgttcaa gtgaaaggaa gagttacatg tctctcactt
    10621 taaatccgtt acacgtctct tcctttaaat caaaagctag aaatgattaa gcttagtgag
    10681 gaaggcatgt tgaaagccaa ggtgggtaga aagcaaggcc ttttccgctg gttagccaag
    10741 ttgtgaatgc aaaggaaatt aaaggttttt ttaaggaaat taaaagtgct gctccactaa
    10801 acacacaaat gataagtgaa actgtcttat tgctgatatg gagaaagttt tagtggttgg
    10861 gacaaaagat caaaccagcc acgacattcc cttaagccaa agcctaatcc aaagcaagtc
    10921 tttaactctt catttctatg aaggctgaga gagatgagga agctgcagaa aaaaagttgg
    10981 aagctaatag aggttggtcc atgaggttta aggaaaaaag ccatctctgt aacacagatg
    11041 tgcaaggtga agcaacaagt gctgatggaa aagctgcagc aaattattac acaaaatctg
    11101 gctaagatag ttgatgaagg tggctatact taacaacaga ttttcagtgg agatgaagta
    11161 gccttttttt ggaataagat accatgtaga actttcatac cttgagagaa atcaatgctt
    11221 ggcttcaaaa cttcaaggat aggctgactc tcttgttagg gactaatgca gttggtggtt
    11281 ttaagttgaa gccattgttc atttgccatt ctgaaaatcc tgaggccctt aagaattatg
    11341 ctaaacctac tctgcctgtg ccctagaaat ggaataacaa agcctgaatt acagcaaatc
    11401 tgtatacaac atggtttact gaatctttta agcccattgt tgagacctgc taccatgtct
    11461 gctaacacaa catccattct gcagcccatg gatcaaggag taatttccac ttgcaagttt
    11521 tattatttaa gaaatatatt ttgcatagtt atagttgcca tagatagtga ttcctctgat
    11581 ggatttgggc aaagtaaaat tttctggaaa gaattcacca ttctaaatgc cattgaggat
    11641 atttatgatt catgggaaga gatcaaaata tcagctttaa cggggttgga agaagttgat
    11701 tccaaccctt gtgggtgatt ttgaagggct caagacttca gcagaagaag taactgcaga
    11761 agttgtggaa atagcaagag agccagaata gaagctgatc ctgagaatat gactgaattg
    11821 ctgtaatctc atgataaatc ttgagcagat gaagagttgc ttcttatgga tgaatacgaa
    11881 agtggtttct tgagatggag tctcctggtg aagtttttgt gaacattgat gataacaaag
    11941 gacttcgcat agcacatcaa cttagttgat taagcagtag cagggtttga gaggattgac
    12001 tccaattttg caagaagttc ttctgtgggt aaaatgctat caaatggcat cacatgctat
    12061 agaagtgttt ggtgtaagga agagtcagtt gatgtcgcaa acctcgttat tttaggaaat
    12121 tgccaaagcc actccagcct ttggcaatca tcaccctgat tagtcaccag ccatcaacat
    12181 tgatataata ctctccacca gtaaaaagat tggttttctg atggctcaga tgattgttgg
    12241 cagtttttat caataacgta tttcttttgt ttttgtttgg gagatggtct ccctctgttg
    12301 cccaggctgg agtgcaatag catgatcata gctcactgca gcctggatcc cctgggctca
    12361 agcaatcctc ctgtcctagc caaccaagta gctaagaaaa cagaggcaca acaccacacc
    12421 tagctaattt attttatttt ttgtagagat ggaatcttgc tatgttgtcc aggctggtct
    12481 ggaactccta ccctcataga atctccctgc ctcagccttc aaagggctgg gactaaaggc
    12541 aggagccacc tcacccaacc aataatgtat ttttaaatta tgtctttttt ttggtggagg
    12601 gggacagagt tttgcccagg ctggagtgca atggcacaat ctcggctcac cgcaacctcc
    12661 acctcccagg ttcaagcgat tctcctacct cagtctccca agtagctggg attacaggca
    12721 tgtaccacta tgcctggcta gttttgtatt tttagtagag acagcgtttc tccatgttgg
    12781 tcaggctggt ctcgaactcc tgacctcagg tgatccgcct gcctcagctt cccaaagtgc
    12841 tgggattaca ggcgtgagcc actgcacctg gcctaaatta tgtacatttt ttatgtatat
    12901 tatgtgaggt ttgccacatc aattgacttt tccttttgat agcattttac ccacagaaga
    12961 acttctttca aaatgggaat caatcctctc aaaccctgcc actgctttat caactaagtt
    13021 gatgtactgt tcaaagtcct ttgttatcag ttaatcaata ttcacaaaat cttcaccagg
    13081 aggactccat ctcaacagac atcataatgc tgttggacac tttatagtat agtataaaca
    13141 aaacttttat atgcactggg aaaccaaaaa cattttgtga cttgctttat tgtgatattt
    13201 gctatattgc agcggtctgg aaccaaaccc ccagtatctc taaggtgtgc ctgtactgtt
    13261 tcttaggtgt cagttttctc tctctgagac gtcattaaga cttttctttc tacattcaga
    13321 tttaaggtcg tacagtatac tttttgctat aacaaagttc attttggact ttaaagttaa
    13381 ccaacttgga ttggtgctta atatttctga gatactctaa ttaagagttc caagtaagat
    13441 aagactactc tggatattta gagaaggcag gtaggggagg aatgaagcca gttctccttg
    13501 gactgatgat ggtgctattt ttcagcacag tctttgactc taaggtgttt tcctttatct
    13561 ttgcttccta cttatcctct gctgttcctt gcttagagtt agaaacagtc tggtagttgc
    13621 agtggttata aaaatatatc ttgatctttg tcttacatct cctgagcagg ccctgtcagg
    13681 gataggtaaa tgtgtcctgt ggattgatac catgatttcc tctcctgaca ggtcccagaa
    13741 tggtgcttag atgactggca tccttctgag aaggcaatgt atcctgatta ctttgccaag
    13801 agagaacagt ggaagaaact gcggagggaa agctgggaac gagaggtgcg ttctacttgt
    13861 acttcgttat tccttagcta tgtagatgga gtgaagtttt gctccccaca agcagaggtc
    13921 cagattccag ccatgacagt tactctgggc ccttgcttct cagatatact ttagtatcta
    13981 agtgtatcaa tttaacattt ataatcacct tacaagtaag ttaaagtgca ttccctggtc
    14041 gttttggctt tgtctttgat tttgcttcag atctccttct ctttagtcct ttgtctgact
    14101 tttttgcctt cttgctatac tttccctcaa cctgcctctc cactattttc ttaggcattt
    14161 ttcagtcatg ttaggatggt cagttatatt tttaaaagct caatcttgct gtagtgtgaa
    14221 gaatatattg gagaggggaa gaggagaagc agagagaccc ttctaggatt attgcagaag
    14281 tttagtcaaa aggttgtcat gactaacact agggtggatg aagtggatat ggaaggaaat
    14341 tgactcagga catcatttgg agggatgtga cgactgactg gatgttgagg gtgggtgagg
    14401 gatggggagg aatcaagaat ggtttctggg gcagggtgcg gtggctcaca cctataatcc
    14461 cagcactttg ggaggccaaa gtgggtgata gcttgaggtc aggagttcat gacgaccagc
    14521 ctgactaaca tggtgaaacc ccatctctac taaaagtaca aataattagc tgggcgtggg
    14581 ggctgtgcac ctgtaatccc agctacttgg gaggctaagg caggagaatt gcttgaatcc
    14641 caggaggtgg aggttgcaat gagctgacat cgcgccactg cattccagcc tgggcgacag
    14701 agcaagactc catcccaaaa gaaaaaaaaa agagagaatg gtttctgggt tttaagcttt
    14761 aacatctgga gtttggtgat gtttactgag atagggaaga ttggggaagg gggcacaggt
    14821 gtcagagcag caatcgagaa ttcattttgg aagtgtgaag ttttgagaat cctgtgagac
    14881 atcaagtgca gatgtcagaa gccaacttca gatgtggaaa tctggaactc ccaggagaga
    14941 tttggcctag aaacagaaac gtgggagttt tcagttttaa aagccatgga gagatgtatg
    15001 gaatccttag gagagagtat agagaaaaaa gaaaaggaaa aaggattctt tgtgcattat
    15061 aattgggcaa aagattgtat ataaatacat ttgggttaga agttttgaag atgacagact
    15121 tgtgtgatag cttccttttt ttagtgaaat aagaggaaaa gcccagaatg agtgggtgca
    15181 gcatatttgg agaatgtgga caagatgtga tagtcatgag gcgaggaaga gcttgctgga
    15241 gagtagtagg atggccaggt ggcaaaggcc catttgaggt tgggaataat cagtgggtaa
    15301 tgactccgat ttgctcccat atcttttttc tctttagcaa tactaagctg tatgagcaca
    15361 ggcctgaggg tatatggttc ggtttgtctg ttgctcagag agtgacaagg gacttgaggt
    15421 tattcgaaag gaagctgttg tcatgatgga atctaagctg aatttaaaaa aaaacaaagt
    15481 caagccagtg ggggcagatg cagagaggga aggccaagtt gggaggatct gtgaattggt
    15541 agtctgagtt tcaaggaggc atttaaacaa atgagtggga aagggagagt gtggttggat
    15601 agtgggatat ttgaattcat gatttggaag ttgtgctggt tctggtgata acactgagga
    15661 tattgacctt tggaagtgtt tggccaaggg ggacccgaag gaagggcact gtggccaaag
    15721 ggggcagtgt tttgtcagaa atgtctcacc gtttagaagc ttgaagtata tgcatggtga
    15781 gccgcaggtg cattctgcaa ttgatcactg ggataacatc aaacaattcc tcctttacac
    15841 atcccatcac ttcaacacta ttaagcagca cgtgtgactg gaggcccagg gcagccacaa
    15901 tccagaatgg gtgatcttct gttaggacat ttatcttggc cataactaga actggaggta
    15961 gtagggcagc acttggcaga actgagcata tttttacatg taattttgtg atttattttt
    16021 gttcttggtt gctaggaagg gggcttagaa ttaacatccc cagcatatta tgaactgagg
    16081 tttcttaatg gtgcttttgg agaacactta gctgagacac aacaataaat gtttttctgg
    16141 tcagtgggtg ggggctgtga caaggagaat tggaatgata gttgtcattt ttctgtctgc
    16201 agaataagag gaggtccaga taatgaggtg atgaaaaggg gtagggggaa gaataggaag
    16261 ggtaggcttt gctatttaaa agaatgggaa aacccttaag gagacttttc taaaagtgtg
    16321 gatatgaaaa taaagcaggt atatgtaaaa aacacttttc aatattgtga tcagatagtg
    16381 tgtaatgtca gtgacacacc atagacataa agctgaccct tcactgcagc agacagattt
    16441 ttatagtcaa ttagattcct ttggtaatga ttccttcctt gcctccttct aggttaagca
    16501 gctgcaggag gaaacgccac ctggtggtcc tttaactgaa gctttgcccc ctgcccgaaa
    16561 ggaaggtgat ttgcccccac tgtggtggta tattgtgacc agaccccggg agcggcccat
    16621 gggaagcggc tccggtagcg ggagcggatc caccgagctc aacttcaagg agtggcaaaa
    16681 ggcctttacc gatatgatgt agaaagagag agacctcatc tttcatgctt gcaagtgaaa
    16741 tatgttacag aacatgcact tgccctaata aaaaatcagt gaaatggtct ctggtatgac
    16801 tgacgttctt ctaagaatta ctgtgatgcc aagtgactgt gccactgtca ttgttttatc
    16861 aatcgtgcat gtaaagaaaa tgacaggaca tgtagtcagt cagctgtgtt ctctgcaata
    16921 aatcactgat agcatatgtt accagtaagg aaaaactgag gcagaaccag attgaggcga
    16981 acaaactctc aagtcaaagg cctaattagc tgtggtaact agaaggaata aacaaagcca
    17041 agaacagaaa tactgaggat ttaaaaataa cctttttggt tttctaaaga tttcactaat
    17101 ggaacttagt ttttattaga agaacaactc tccctaagaa gtacatagtc aacaaataag
    17161 catggaattt gtggagttct gtcaggggtg gaggtttgat ggttggggtt aaatggtcca
    17221 atctgggata aaactttgtg gcgcttattt tagaagcagt tttccagctt tgcttctgct
    17281 accgtagctt ggattgtctg cggactcaaa ggtaagggaa taacagaact ccttaaatgt
    17341 ttcagaagct ttgtttttgt tttttaaaga taaagcagtt tttaaaactt tcaacccaaa
    17401 acagctgctt ttagttcttg atccctcatt agctagaact gttttcaaat agtttcaggc
    17461 aactaatgat cttatagcca tcacaaaaat attttgtcac ttttctacgt aagctcaatt
    17521 tgagtcccag tccatcttct aacatttgac taagacctca aactgaattg taacaagact
    17581 tgaaatacag gactttttta atctagtggt tctttattta taacgtaggc tgaagcaatt
    17641 gttacaatgt aggagggaga cacattacac aattgttatt tatacaagtt tagaacaaaa
    17701 aactgcacag ggagaggtca actctcagta caaactagcg tttaaaccaa gcggcgactg
    17761 agatgtccta aatgcacagc gacggattcg cgctatttag aaagagagag caatatttca
    17821 agaatgcatg cgtcaatttt acgcagacta tctttctagg gttaatctag ctgcatcagg
    17881 atcatatcgt cgggtctttt ttccggctca gtcatcgccc aagctggcgc tatctgggca
    17941 tcggggagga agaagcccgt gccttttccc gcgaggttga agcggcatgg aaagagtttg
    18001 ccgaggatga ctgctgctgc attgacgttg agcgaaaacg cacgtttacc atgatgattc
    18061 gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt cgttcaggcc acctgggata
    18121 ccagttcgtc gcggcttttc cggacacagt tccggatggt cagcccgaag cgcatcagca
    18181 acccgaacaa taccggcgac agccggaact gccgtgccgg tgtgcagatt aatgacagcg
    18241 gtgcggcgct gggatattac gtcagcgagg acgggtatcc tggctggatg ccgcagaaat
    18301 ggacatggat accccgtgag ttacccggcg ggcgcgcttg gcgtaatcat ggtcatagct
    18361 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
    18421 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
    18481 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
    18541 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
    18601 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    18661 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    18721 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    18781 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    18841 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    18901 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    18961 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    19021 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    19081 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    19141 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
    19201 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    19261 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    19321 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    19381 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    19441 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    19501 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    19561 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    19621 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    19681 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    19741 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    19801 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    19861 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    19921 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    19981 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    20041 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    20101 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    20161 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    20221 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    20281 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    20341 aataagggcg acacggaaat gttgaatact cat
//
