LOCUS       Exported               19455 bp ds-DNA     circular SYN 22-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19455)
  AUTHORS   The Weissman lab
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 6, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..19455
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    874..936
                     /label=5'ITR
     misc_feature    complement(1400..1993)
                     /label=WPRE
                     /label=WPRE; Woodchuck post-transcriptional
     primer_bind     complement(1893..1912)
                     /label=HKO083
     CDS             complement(2016..2615)
                     /label=Puro (-)
     promoter        complement(2628..3130)
                     /label=PGK (-)
     misc_feature    3137..16821
                     /label=NDUFB9-endogenous locus
     misc_feature    5066..5150
                     /label=5'UTR
     misc_feature    5154..5156
                     /label=deletion of 72bp MTS region
     misc_feature    9145..9156
                     /label=NDUFB9
     misc_feature    9145..9156
                     /label=variant_bps
     misc_feature    9145..9156
                     /label=variation
     misc_feature    15785..15878
                     /label=3'UTR
     misc_feature    16909..16943
                     /label=3'ITR
     primer_bind     complement(17434..17450)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    17458..17474
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(17482..17512)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    17527..17548
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(17836..18424)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(18595..19455)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc agggtttatt acagggacag cagagatcca
     1381 gtttggttaa ttagctagcg atgcggggag gcggcccaaa gggagatccg actcgtctga
     1441 gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg
     1501 tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg
     1561 gcaacacagg cgagcagcca aggaaaggac gatgatttcc ccgacaacac cacggaattg
     1621 tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc
     1681 gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca
     1741 atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg
     1801 acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa
     1861 atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat
     1921 agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc
     1981 agaggttgat taccgataag cttgatatcg aattcttaac tggcaccggg cttgcgggtc
     2041 atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc
     2101 cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg
     2161 cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg
     2221 tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc
     2281 aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc
     2341 gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc
     2401 acggcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg
     2461 aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc
     2521 gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg
     2581 cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg tggtaccccc tggggagaga
     2641 ggtcggtgat tcggtcaacg agggagccga ctgccgacgt gcgctccgga ggcttgcaga
     2701 atgcggaaca ccgcgcgggc aggaacaggg cccacactac cgccccacac cccgcctccc
     2761 gcaccgcccc ttcccggccg ctgctctcgg cgcgccctgc tgagcagccg ctattggcca
     2821 cagcccatcg cggtcggcgc gctgccattg ctccctggcg ctgtccgtct gcgagggtac
     2881 tagtgagacg tgcggcttcc gtttgtcacg tccggcacgc cgcgaaccgc aaggaacctt
     2941 cccgacttag gggcggagca ggaagcgtcg ccggggggcc cacaagggta gcggcgaaga
     3001 tccgggtgac gctgcgaacg gacgtgaaga atgtgcgaga cccagggtcg gcgccgctgc
     3061 gtttcccgga accacgccca gagcagccgc gtccctgcgc aaacccaggg ctgccttgga
     3121 aaaggcgcaa acgcgtccca aacagtcatt gtactacgcc cctaggaaaa aaatataaat
     3181 accataccag tatctgcctt aagagtttga tctagtggga aaggcatact catatttaat
     3241 tataaataat gtggtataag ttttaaggag tgagtaggca gacaggcaac agggtagttg
     3301 gtaggcagag gaatattttg gccccaaact gccactctaa aaccttgtca gaggtaacaa
     3361 acagcatgca caagtgttta tagagcagcc cagggatggg ctagaaaagt gggcaggact
     3421 ggaccacaga agggctttgt atggtgtgta agcatttagg ctatcctgag gagcgtaaat
     3481 gaaagatttg aagcaaaaga aacatttagc tgatttgatt tttagataca ccaaaatgga
     3541 gtctacatgg aggatggatt taagacatta acttgaggca ggaatttggt tgtataacat
     3601 aaacacactt ttaaaaagtt atgtggcctt tctttacgga gtacttcatg gctctttatt
     3661 ccatctccct catcaattat atcaaaggtt aggatggaaa ggctaagaca cggcaatgtc
     3721 ttatttgaca acccctcccc aaacccctac tccaggcttt cccacagcaa tttcaaaacc
     3781 tttttcctgg cacttaacat attctcccct gcatttgctt ttgactgtac agtacatagg
     3841 tcttaggtct ccagtctgaa aactgcacca ctagatttta cagttccaca ggacttagca
     3901 caacgtcttt gaaaaaaaaa aatactgctt aaataactct cagatctaaa tgtataatcc
     3961 taatctcttg attgccagac cacattccca attgcccaga tatctctacc tggatgtcct
     4021 ataattatct caaacttaac ctaatccaaa tggacctggg tacctctctt caccgccaaa
     4081 ctagtcatgc ttccatttca tatttaagta cttttccaaa ccctgagtca tctttgttca
     4141 ctctctacta ccggccatga acactcagct gctaaatgct gcacacatct cttccatttc
     4201 tctctctttt ccattaccct agtttaagct cttgaaatgc ttccctacct ctccctattt
     4261 cccacaaaac ctacataata gctatcagga ttaccttgct aaagcagagg tgtgatcact
     4321 gtcatttccc accttcaggt agctggaaac cccacaaaac gaggatcatg cccatttaaa
     4381 tcccgcagca ttctccctct cgttctactg atgccgactg gatcattgta cttgccaagt
     4441 acagtttgtt tttctcccca ttacttgtgt tttctagttt ctccaccaga tggtgatgac
     4501 agtacaactg ggagctttgt gccctgtgtt cctaggccag tgccaggccc acttgaggcg
     4561 ctcactaact atgatatgaa cgaatgaatg cttgagtgaa ttggtcccca gaagcggtca
     4621 gcctctccgc caaggcagag gggaagggcc gggaggtgca cgtgggatga atggaaacat
     4681 agtgattggc ctttttggag ttttcttctt aaaaaactga atacattaca gatgttgaaa
     4741 acagcacaaa aatcataact gtacggcttg ttaattttta caaataaata caccctcgtg
     4801 aatccagaac ctagatcaag aaccagagca ttaccagcac ccagaaacct ccccgcgccc
     4861 tcctccactg agcggccctt tcaaccctta caatgccgga gggcgcaatt cctgggtcct
     4921 gtgaccttgg tgaccgacgc ccgggacaag tcagaaagac ccaagggcgt ggaaaacgct
     4981 cctcttaccg ataaacttga agcgactcat gactgcgcat ggaggacctc cccagcggaa
     5041 gcggaagtgg ccgccggcaa ctccgccctt ccggctggcc ccgctcagtc acccgcagca
     5101 ggcgtgcagt ttcccggctc tccgcgcggc cggggaaggt cagcgccgta atgcacctcg
     5161 agtcgtggtg cgtccagagg taagggatgg ggacccagga ctcggggagg tgaccctcgg
     5221 ggccccatgg aggtggagag gcccctgggt acctggaggt tcaaggactt cgggaacgga
     5281 attggaaggt ggcctctcgc ttcacagaat cctatggtag acccgggttc aaatccaggc
     5341 ttcgccactt atggccggga gaccttgtag agttgcttgt cgggtctggg cgtcagcttt
     5401 ttgtttagta aaacggacgc acgaccagcg cagagggtcg caggaaaatt aagtgaaata
     5461 aaggaaagtg cctaaaattg taattgtcat gggtccagcg ctcgctcaat cgttttcttc
     5521 acgatcttct cctttccaat attaacagca ataaactatg cattattttc ttcagtctgc
     5581 aaaactctag gagttaggaa ttatcttaag cttattttac agataagaag atgagcacag
     5641 ctggtaactt gcgcaaggtg atagagtttc taagtgtctg agccagggtt aaaatttagg
     5701 tctttctcca aagcatcatc attctcccac ccctcccatt agcttttctc tttttagctt
     5761 catctagtcc ttttcattac ctttacatct ttttcacctc ttacataatt ctctttcgac
     5821 ctctatccgt ctgagactag tctgagaaca tagtgttaaa atgtgtagat tttaatcacc
     5881 taaattcatt gcagggtggg ctaacaaccc aagatgtgtg agtgcctcgc ctccactcca
     5941 gtcttacgac tctccaaacg tagttttata agaaggcctt ctgggaaaaa aactctatgg
     6001 aaaatggagt aggcctggaa gaattacaga gggtaccctc gaagcccaga ttgcatatat
     6061 gtgactatta gaaaatcact gacatacttc tcttgatttg gcaggaagat ggccaagtac
     6121 aatgtgatgg gattgttagt ttcttccttg agttttcctt atttatttat tgatttgaca
     6181 gaatttgcaa ttcagattct atgctctatg ctaaatatat aaatgttagt aaatccattt
     6241 ttgagcagca gcagctcacg gattagtggg gaaaatggac atgtaaacaa atcgatgatg
     6301 aaggaagttg agccatcagg gattcaggta tcccaacaca aatggtaaat gtttgaggtg
     6361 atgaatatcc aaattaccct gatttgatca ttatacatta tatggatgta ttaaaatatc
     6421 acatgtaccc cataaatagg tacaataatt atgtgtcaaa aaaagttaaa catgaaaaaa
     6481 agaattcaag caatactgaa ctatagctgg gaatactaaa gtccatagtt ctagaggaaa
     6541 atgatataaa ctagctacta tgacagttat ggctcagcat tttttagatc tgacattctt
     6601 tctcaatcct tgccttaatt tcttcctcca tttgccctca ttgtgctgtt ctctgtggtt
     6661 ccatcctagg catactgtgt tgttgtatgt actcgggcag cctcatttag tgctgtcacc
     6721 acctgcgctc atttgtctct agagctgtag ttcgaattgc ttgttagatc acgactgttg
     6781 gctgggcatg atggctcacg cctataatcc cagtactttg ggaggctgag gtgagtggat
     6841 cacctgaggc caggagttca acagcctggc ctacatgtga aaccgcgtct ctactaaaaa
     6901 tacaaaaatt agccaggcgt ggtggtgggt gcctgtaatc ccagctactc atgaggctga
     6961 ggcagaagaa ccgcttgaac ctgggaggca gaggctgcag tgagccgaga tagccccatt
     7021 gcactccagc ctgggcgaca agagtgaaac tttgtctcaa aaaataataa taaaaaaaaa
     7081 attgaaaaga tcaccaaccg ttctctcaat ctccatttat ctaaattctt tgaaatcaaa
     7141 cttgttttcc tgtcactccc cattctgact cttttcccaa attccctgcc tccgctaatg
     7201 gtattattag taattcagtt actgagcttt aagtctcaga attgttctca agtatttgct
     7261 ccttattcct ttgccaataa cacacatact catacaccat gtataattag acactggttt
     7321 ttataaattc tctttccagt atgatattag taactgacat tatttaacac ttgggatgta
     7381 ccaggcactt tttaaagtgt gtttatctta ttgactgttt atgacaacct taagatatac
     7441 acactgttat tgccactgtt ttacagagga ggaacccgtg gcctagaaga ctttttttgt
     7501 ttgttttttt gttagacgga gtttcgctta tgttgcccag gctggagtgc agtggcgcga
     7561 tcttggctca ccgcaagttc cgtctcccgg gttcaagcaa ttctcctgcc tcagtctccc
     7621 gagtagctgg gattacaggc atgcgccacc acgcctggct aatttttgta tttttagtag
     7681 agacggggtt ttgccatgtt ggccaggctg gtcttgaact cctgacctca ggtgacctcc
     7741 acctcagcct cccaaagtgc tgggattaca ggcgtgagcc accgtgcctg gcctttttgt
     7801 tgttgttgtt gttgtttttt tttgagatga agtctcgctc ttgtccccca ggctggagtg
     7861 caaatggcac aatctcgact cactgcaacc tctgcctccc gggttcaagc tattctcctg
     7921 ccttggcccc cagagtagct gggattacag gtgcctgcca ccacgcccgg ctagtttttg
     7981 tatttttagc tgagacggga tttcaccatg ttggccaggc tggtgtagaa ctcctcacct
     8041 cagatgatcc ccctgccttg gcctcccaaa gtgctgggat tacaggcgtg agccaccatg
     8101 cccggcctca tttttgtatt tttagtagag acggggtttc tccatgttga tcaggcttgt
     8161 cttgaactcc agacctcagg tgatccacac gccttggcct cccaacgtgc tgggattaca
     8221 ggtgtgagcc accgcacctg accagaagac agtaacttac cccaagttac acagctagta
     8281 gtggcagagc taagattcag gcccatacag tctgactgga gagcctattc tcataacttg
     8341 gaagtctcaa tgtctcccct gtctcctgtc accatcacag ctccgtactt tcaggccctc
     8401 gtcagctgtg tcctagatta ttgtcaaaac ctcctaactg atctttgccc agtttcttta
     8461 gaacttgact ttaacttgcc taagtttatg cagctagtgt cagagtcagg atacaaacct
     8521 agctctgtat gagctaagca atactgtttc tgccaagtaa cacctttctt attcatcttt
     8581 gtatctccag aatttagcat agtgactagc acaagataga aggaaatgga agttgtcatt
     8641 caatgaatgt tgaatgatcc tgcagaggtt atagcttttc tcttgcgaat gctcttttct
     8701 tttttttttt tttttttttt gtttaaatcc tcattccatt gttggatgta agaggagaaa
     8761 aatggttagg gtatatattt aatacagtat ttttttgctg ctgctgtttg gtgggctgta
     8821 gaggaagcag ccatgatgat acggctgctc cattgggagg gttggggtag gtccactgcc
     8881 catccagaag gtatcagaag tgcagcctcc tgtcagacag caacactgac cacgtgagta
     8941 ggtaacattt ctaatcttca aaatcatttt ggtttaagag acaaataccg atactttgct
     9001 tgtttgatga gagcccggtt tgaagaacat aagaatgaaa aggatatggc gaaggccacc
     9061 cagctgctga aggaggccga ggaagaattc tggtaccgtc agcatccaca gccatacatc
     9121 ttccctgact ctcctggggg caccagctat gagcggtacg attgctacaa ggtaggtgag
     9181 aattatgatg actgccttct gagaaataga ctttgtttct tcagtcccac acctcagcct
     9241 cagaaatcac ttgcccttca aggggtagct aggagactta tttcctgaca gttgtcagat
     9301 gcaggcctac ctcattttat tgcattttgt tttattgtgc ttggcagata ttgtattttc
     9361 acagattgaa agtttgtggc aaccccgtgc tgagcaagtc tgtgggcacc atttttccaa
     9421 cagcatgtgc tcactccctg tctctgtttc tcagtttggc aattcttgca atatttcaaa
     9481 ctttttttta ttattactct atctacacac agttgtggtg aactgtgatc agtcaccttt
     9541 gatgttacta ttgtcattgt tttggggcac aaaaaaacca tgcccacata agacagcaga
     9601 cttaatcgat aaatgtgcgt gtgctgactg tttcaccgac caatagttcc tccgcctcta
     9661 tccctctcct cagggttccc tattccctaa gacgcagcaa cagtgaaatt aggacaatta
     9721 acgaccatac catagcctct aagtgttcaa gtgaaaggaa gagttacatg tctctcactt
     9781 taaatccgtt acacgtctct tcctttaaat caaaagctag aaatgattaa gcttagtgag
     9841 gaaggcatgt tgaaagccaa ggtgggtaga aagcaaggcc ttttccgctg gttagccaag
     9901 ttgtgaatgc aaaggaaatt aaaggttttt ttaaggaaat taaaagtgct gctccactaa
     9961 acacacaaat gataagtgaa actgtcttat tgctgatatg gagaaagttt tagtggttgg
    10021 gacaaaagat caaaccagcc acgacattcc cttaagccaa agcctaatcc aaagcaagtc
    10081 tttaactctt catttctatg aaggctgaga gagatgagga agctgcagaa aaaaagttgg
    10141 aagctaatag aggttggtcc atgaggttta aggaaaaaag ccatctctgt aacacagatg
    10201 tgcaaggtga agcaacaagt gctgatggaa aagctgcagc aaattattac acaaaatctg
    10261 gctaagatag ttgatgaagg tggctatact taacaacaga ttttcagtgg agatgaagta
    10321 gccttttttt ggaataagat accatgtaga actttcatac cttgagagaa atcaatgctt
    10381 ggcttcaaaa cttcaaggat aggctgactc tcttgttagg gactaatgca gttggtggtt
    10441 ttaagttgaa gccattgttc atttgccatt ctgaaaatcc tgaggccctt aagaattatg
    10501 ctaaacctac tctgcctgtg ccctagaaat ggaataacaa agcctgaatt acagcaaatc
    10561 tgtatacaac atggtttact gaatctttta agcccattgt tgagacctgc taccatgtct
    10621 gctaacacaa catccattct gcagcccatg gatcaaggag taatttccac ttgcaagttt
    10681 tattatttaa gaaatatatt ttgcatagtt atagttgcca tagatagtga ttcctctgat
    10741 ggatttgggc aaagtaaaat tttctggaaa gaattcacca ttctaaatgc cattgaggat
    10801 atttatgatt catgggaaga gatcaaaata tcagctttaa cggggttgga agaagttgat
    10861 tccaaccctt gtgggtgatt ttgaagggct caagacttca gcagaagaag taactgcaga
    10921 agttgtggaa atagcaagag agccagaata gaagctgatc ctgagaatat gactgaattg
    10981 ctgtaatctc atgataaatc ttgagcagat gaagagttgc ttcttatgga tgaatacgaa
    11041 agtggtttct tgagatggag tctcctggtg aagtttttgt gaacattgat gataacaaag
    11101 gacttcgcat agcacatcaa cttagttgat taagcagtag cagggtttga gaggattgac
    11161 tccaattttg caagaagttc ttctgtgggt aaaatgctat caaatggcat cacatgctat
    11221 agaagtgttt ggtgtaagga agagtcagtt gatgtcgcaa acctcgttat tttaggaaat
    11281 tgccaaagcc actccagcct ttggcaatca tcaccctgat tagtcaccag ccatcaacat
    11341 tgatataata ctctccacca gtaaaaagat tggttttctg atggctcaga tgattgttgg
    11401 cagtttttat caataacgta tttcttttgt ttttgtttgg gagatggtct ccctctgttg
    11461 cccaggctgg agtgcaatag catgatcata gctcactgca gcctggatcc cctgggctca
    11521 agcaatcctc ctgtcctagc caaccaagta gctaagaaaa cagaggcaca acaccacacc
    11581 tagctaattt attttatttt ttgtagagat ggaatcttgc tatgttgtcc aggctggtct
    11641 ggaactccta ccctcataga atctccctgc ctcagccttc aaagggctgg gactaaaggc
    11701 aggagccacc tcacccaacc aataatgtat ttttaaatta tgtctttttt ttggtggagg
    11761 gggacagagt tttgcccagg ctggagtgca atggcacaat ctcggctcac cgcaacctcc
    11821 acctcccagg ttcaagcgat tctcctacct cagtctccca agtagctggg attacaggca
    11881 tgtaccacta tgcctggcta gttttgtatt tttagtagag acagcgtttc tccatgttgg
    11941 tcaggctggt ctcgaactcc tgacctcagg tgatccgcct gcctcagctt cccaaagtgc
    12001 tgggattaca ggcgtgagcc actgcacctg gcctaaatta tgtacatttt ttatgtatat
    12061 tatgtgaggt ttgccacatc aattgacttt tccttttgat agcattttac ccacagaaga
    12121 acttctttca aaatgggaat caatcctctc aaaccctgcc actgctttat caactaagtt
    12181 gatgtactgt tcaaagtcct ttgttatcag ttaatcaata ttcacaaaat cttcaccagg
    12241 aggactccat ctcaacagac atcataatgc tgttggacac tttatagtat agtataaaca
    12301 aaacttttat atgcactggg aaaccaaaaa cattttgtga cttgctttat tgtgatattt
    12361 gctatattgc agcggtctgg aaccaaaccc ccagtatctc taaggtgtgc ctgtactgtt
    12421 tcttaggtgt cagttttctc tctctgagac gtcattaaga cttttctttc tacattcaga
    12481 tttaaggtcg tacagtatac tttttgctat aacaaagttc attttggact ttaaagttaa
    12541 ccaacttgga ttggtgctta atatttctga gatactctaa ttaagagttc caagtaagat
    12601 aagactactc tggatattta gagaaggcag gtaggggagg aatgaagcca gttctccttg
    12661 gactgatgat ggtgctattt ttcagcacag tctttgactc taaggtgttt tcctttatct
    12721 ttgcttccta cttatcctct gctgttcctt gcttagagtt agaaacagtc tggtagttgc
    12781 agtggttata aaaatatatc ttgatctttg tcttacatct cctgagcagg ccctgtcagg
    12841 gataggtaaa tgtgtcctgt ggattgatac catgatttcc tctcctgaca ggtcccagaa
    12901 tggtgcttag atgactggca tccttctgag aaggcaatgt atcctgatta ctttgccaag
    12961 agagaacagt ggaagaaact gcggagggaa agctgggaac gagaggtgcg ttctacttgt
    13021 acttcgttat tccttagcta tgtagatgga gtgaagtttt gctccccaca agcagaggtc
    13081 cagattccag ccatgacagt tactctgggc ccttgcttct cagatatact ttagtatcta
    13141 agtgtatcaa tttaacattt ataatcacct tacaagtaag ttaaagtgca ttccctggtc
    13201 gttttggctt tgtctttgat tttgcttcag atctccttct ctttagtcct ttgtctgact
    13261 tttttgcctt cttgctatac tttccctcaa cctgcctctc cactattttc ttaggcattt
    13321 ttcagtcatg ttaggatggt cagttatatt tttaaaagct caatcttgct gtagtgtgaa
    13381 gaatatattg gagaggggaa gaggagaagc agagagaccc ttctaggatt attgcagaag
    13441 tttagtcaaa aggttgtcat gactaacact agggtggatg aagtggatat ggaaggaaat
    13501 tgactcagga catcatttgg agggatgtga cgactgactg gatgttgagg gtgggtgagg
    13561 gatggggagg aatcaagaat ggtttctggg gcagggtgcg gtggctcaca cctataatcc
    13621 cagcactttg ggaggccaaa gtgggtgata gcttgaggtc aggagttcat gacgaccagc
    13681 ctgactaaca tggtgaaacc ccatctctac taaaagtaca aataattagc tgggcgtggg
    13741 ggctgtgcac ctgtaatccc agctacttgg gaggctaagg caggagaatt gcttgaatcc
    13801 caggaggtgg aggttgcaat gagctgacat cgcgccactg cattccagcc tgggcgacag
    13861 agcaagactc catcccaaaa gaaaaaaaaa agagagaatg gtttctgggt tttaagcttt
    13921 aacatctgga gtttggtgat gtttactgag atagggaaga ttggggaagg gggcacaggt
    13981 gtcagagcag caatcgagaa ttcattttgg aagtgtgaag ttttgagaat cctgtgagac
    14041 atcaagtgca gatgtcagaa gccaacttca gatgtggaaa tctggaactc ccaggagaga
    14101 tttggcctag aaacagaaac gtgggagttt tcagttttaa aagccatgga gagatgtatg
    14161 gaatccttag gagagagtat agagaaaaaa gaaaaggaaa aaggattctt tgtgcattat
    14221 aattgggcaa aagattgtat ataaatacat ttgggttaga agttttgaag atgacagact
    14281 tgtgtgatag cttccttttt ttagtgaaat aagaggaaaa gcccagaatg agtgggtgca
    14341 gcatatttgg agaatgtgga caagatgtga tagtcatgag gcgaggaaga gcttgctgga
    14401 gagtagtagg atggccaggt ggcaaaggcc catttgaggt tgggaataat cagtgggtaa
    14461 tgactccgat ttgctcccat atcttttttc tctttagcaa tactaagctg tatgagcaca
    14521 ggcctgaggg tatatggttc ggtttgtctg ttgctcagag agtgacaagg gacttgaggt
    14581 tattcgaaag gaagctgttg tcatgatgga atctaagctg aatttaaaaa aaaacaaagt
    14641 caagccagtg ggggcagatg cagagaggga aggccaagtt gggaggatct gtgaattggt
    14701 agtctgagtt tcaaggaggc atttaaacaa atgagtggga aagggagagt gtggttggat
    14761 agtgggatat ttgaattcat gatttggaag ttgtgctggt tctggtgata acactgagga
    14821 tattgacctt tggaagtgtt tggccaaggg ggacccgaag gaagggcact gtggccaaag
    14881 ggggcagtgt tttgtcagaa atgtctcacc gtttagaagc ttgaagtata tgcatggtga
    14941 gccgcaggtg cattctgcaa ttgatcactg ggataacatc aaacaattcc tcctttacac
    15001 atcccatcac ttcaacacta ttaagcagca cgtgtgactg gaggcccagg gcagccacaa
    15061 tccagaatgg gtgatcttct gttaggacat ttatcttggc cataactaga actggaggta
    15121 gtagggcagc acttggcaga actgagcata tttttacatg taattttgtg atttattttt
    15181 gttcttggtt gctaggaagg gggcttagaa ttaacatccc cagcatatta tgaactgagg
    15241 tttcttaatg gtgcttttgg agaacactta gctgagacac aacaataaat gtttttctgg
    15301 tcagtgggtg ggggctgtga caaggagaat tggaatgata gttgtcattt ttctgtctgc
    15361 agaataagag gaggtccaga taatgaggtg atgaaaaggg gtagggggaa gaataggaag
    15421 ggtaggcttt gctatttaaa agaatgggaa aacccttaag gagacttttc taaaagtgtg
    15481 gatatgaaaa taaagcaggt atatgtaaaa aacacttttc aatattgtga tcagatagtg
    15541 tgtaatgtca gtgacacacc atagacataa agctgaccct tcactgcagc agacagattt
    15601 ttatagtcaa ttagattcct ttggtaatga ttccttcctt gcctccttct aggttaagca
    15661 gctgcaggag gaaacgccac ctggtggtcc tttaactgaa gctttgcccc ctgcccgaaa
    15721 ggaaggtgat ttgcccccac tgtggtggta tattgtgacc agaccccggg agcggcccat
    15781 gtagaaagag agagacctca tctttcatgc ttgcaagtga aatatgttac agaacatgca
    15841 cttgccctaa taaaaaatca gtgaaatggt ctctggtatg actgacgttc ttctaagaat
    15901 tactgtgatg ccaagtgact gtgccactgt cattgtttta tcaatcgtgc atgtaaagaa
    15961 aatgacagga catgtagtca gtcagctgtg ttctctgcaa taaatcactg atagcatatg
    16021 ttaccagtaa ggaaaaactg aggcagaacc agattgaggc gaacaaactc tcaagtcaaa
    16081 ggcctaatta gctgtggtaa ctagaaggaa taaacaaagc caagaacaga aatactgagg
    16141 atttaaaaat aacctttttg gttttctaaa gatttcacta atggaactta gtttttatta
    16201 gaagaacaac tctccctaag aagtacatag tcaacaaata agcatggaat ttgtggagtt
    16261 ctgtcagggg tggaggtttg atggttgggg ttaaatggtc caatctggga taaaactttg
    16321 tggcgcttat tttagaagca gttttccagc tttgcttctg ctaccgtagc ttggattgtc
    16381 tgcggactca aaggtaaggg aataacagaa ctccttaaat gtttcagaag ctttgttttt
    16441 gttttttaaa gataaagcag tttttaaaac tttcaaccca aaacagctgc ttttagttct
    16501 tgatccctca ttagctagaa ctgttttcaa atagtttcag gcaactaatg atcttatagc
    16561 catcacaaaa atattttgtc acttttctac gtaagctcaa tttgagtccc agtccatctt
    16621 ctaacatttg actaagacct caaactgaat tgtaacaaga cttgaaatac aggacttttt
    16681 taatctagtg gttctttatt tataacgtag gctgaagcaa ttgttacaat gtaggaggga
    16741 gacacattac acaattgtta tttatacaag tttagaacaa aaaactgcac agggagaggt
    16801 caactctcag tacaaactag cgtttaaacc aagcggcgac tgagatgtcc taaatgcaca
    16861 gcgacggatt cgcgctattt agaaagagag agcaatattt caagaatgca tgcgtcaatt
    16921 ttacgcagac tatctttcta gggttaatct agctgcatca ggatcatatc gtcgggtctt
    16981 ttttccggct cagtcatcgc ccaagctggc gctatctggg catcggggag gaagaagccc
    17041 gtgccttttc ccgcgaggtt gaagcggcat ggaaagagtt tgccgaggat gactgctgct
    17101 gcattgacgt tgagcgaaaa cgcacgttta ccatgatgat tcgggaaggt gtggccatgc
    17161 acgcctttaa cggtgaactg ttcgttcagg ccacctggga taccagttcg tcgcggcttt
    17221 tccggacaca gttccggatg gtcagcccga agcgcatcag caacccgaac aataccggcg
    17281 acagccggaa ctgccgtgcc ggtgtgcaga ttaatgacag cggtgcggcg ctgggatatt
    17341 acgtcagcga ggacgggtat cctggctgga tgccgcagaa atggacatgg ataccccgtg
    17401 agttacccgg cgggcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
    17461 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
    17521 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
    17581 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
    17641 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
    17701 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
    17761 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
    17821 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    17881 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    17941 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    18001 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    18061 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    18121 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    18181 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    18241 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
    18301 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    18361 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    18421 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    18481 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    18541 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    18601 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    18661 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    18721 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    18781 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    18841 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    18901 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    18961 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    19021 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    19081 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    19141 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    19201 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    19261 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    19321 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    19381 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    19441 atgttgaata ctcat
//
