LOCUS       Exported               14958 bp ds-DNA     circular SYN 22-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14958)
  AUTHORS   The Weissman lab
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 6, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14958
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    874..936
                     /label=5'ITR
     misc_feature    complement(1400..1993)
                     /label=WPRE
                     /label=WPRE; Woodchuck post-transcriptional
     primer_bind     complement(1893..1912)
                     /label=HKO083
     CDS             complement(2016..2615)
                     /label=Puro (-)
     promoter        complement(2628..3130)
                     /label=PGK (-)
     misc_feature    3137..12324
                     /label=COX7A2
     misc_feature    4187..5186
                     /label=Promoter 1000bp upstream
     misc_feature    complement(5187..5270)
                     /label=ACTB 5UTR
     misc_feature    5271..5288
                     /label=Exon1
     misc_feature    5271..5288
                     /label=MTS part 1
     misc_feature    6170..6184
                     /label=missing in clone
     misc_feature    7740..7829
                     /label=Exon2
     misc_feature    7740..7790
                     /label=MTSpart2
     misc_feature    7772..7780
                     /label=mutations
     misc_feature    8620..8704
                     /label=Exon 3
     misc_feature    11017..11075
                     /label=Exon 4
     misc_feature    11076..11218
                     /label=3' UTR
     misc_feature    11219..12218
                     /label=1000 bp downstream
     misc_feature    12412..12446
                     /label=3'ITR
     primer_bind     complement(12937..12953)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12961..12977
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12985..13015)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13030..13051
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(13339..13927)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(14098..14958)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc agggtttatt acagggacag cagagatcca
     1381 gtttggttaa ttagctagcg atgcggggag gcggcccaaa gggagatccg actcgtctga
     1441 gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg
     1501 tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg
     1561 gcaacacagg cgagcagcca aggaaaggac gatgatttcc ccgacaacac cacggaattg
     1621 tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc
     1681 gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca
     1741 atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg
     1801 acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa
     1861 atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat
     1921 agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc
     1981 agaggttgat taccgataag cttgatatcg aattcttaac tggcaccggg cttgcgggtc
     2041 atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc
     2101 cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg
     2161 cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg
     2221 tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc
     2281 aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc
     2341 gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc
     2401 acggcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg
     2461 aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc
     2521 gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg
     2581 cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg tggtaccccc tggggagaga
     2641 ggtcggtgat tcggtcaacg agggagccga ctgccgacgt gcgctccgga ggcttgcaga
     2701 atgcggaaca ccgcgcgggc aggaacaggg cccacactac cgccccacac cccgcctccc
     2761 gcaccgcccc ttcccggccg ctgctctcgg cgcgccctgc tgagcagccg ctattggcca
     2821 cagcccatcg cggtcggcgc gctgccattg ctccctggcg ctgtccgtct gcgagggtac
     2881 tagtgagacg tgcggcttcc gtttgtcacg tccggcacgc cgcgaaccgc aaggaacctt
     2941 cccgacttag gggcggagca ggaagcgtcg ccggggggcc cacaagggta gcggcgaaga
     3001 tccgggtgac gctgcgaacg gacgtgaaga atgtgcgaga cccagggtcg gcgccgctgc
     3061 gtttcccgga accacgccca gagcagccgc gtccctgcgc aaacccaggg ctgccttgga
     3121 aaaggcgcaa acgcgttggt gagggacgga ttaactatgt taaatgctgc taatagatgg
     3181 agaaggtgaa aattgagatt gacagtttat ttagcaacct ggaagctatt ggtgattcta
     3241 aacaagagca gtaaaagcct gcccaaaatg ggatcaaaaa aagattagga ggaggagaag
     3301 gagagacaag tataaagaaa tctataggat ggtaacggta atcaaaggag tttgttcgtt
     3361 tgtttttaat ttgggaatgt gttaatagat ggaatgatcc agtaaagggg gaaattgtac
     3421 tgaataacat gcatacaaga atctatgtct actttaggat ctatttaatg tgaattcatg
     3481 gctttttttt tttttttgat atgaagtctc actctgtcgc ccaggctgga gtgcagtgga
     3541 gcgatcttgg ctcactgcaa gctccacctt ctgggttcac gccattctcc tgcctcaacc
     3601 tcctgagtag ctgggactac aggcgcccgc caccacgccc gccttatttt ttgtattttt
     3661 agtagagaca gggtttcacg gctttagcca agatggtctg gatctcctga cctcatgatc
     3721 cgcccgcctc ggcctcccaa agtactggga ttacaggcgt gagccactgc gcctggcctc
     3781 atggctcttt ttggacaggg tcataagctt cttagatctg tcacccaatg taattaattg
     3841 atgtattggt taatactgtc tttagcagtg ctaggggatt ggtaagaaat ccacatcatc
     3901 tctgcgcttg ggaaatttac aatctagagt tggctaagtg gagaaaacat aagagagcac
     3961 atacaactaa caaattcaga ttatggacaa tgtacttaaa aaacccccac gccctagcta
     4021 ttggcacgtg gtaattttgc ccagagtttt ggttatatgc ataatccaga ggggtgtaga
     4081 aaaccaggtc ctttcagtac caatctaagg aagacatgaa ttggggtgtt tataaacctt
     4141 ggtgaggaag agaattttaa ctgggactgg taacttggtg ggcccattga catacagtag
     4201 attgtgaaga agaaaacccc aaggattata aggaatagtt ttaatattac taatttttat
     4261 cctttgctga aactatgggt ttcttatttg aaatggcaat caaataattt gtaagagttt
     4321 gtagaaagtg gaaagattat tgtaatacag ggtagatatt tgcatttgta catacttgag
     4381 ggtaagtaca agaaataaac acttgtaatg gggattactt aagcaaaatg gatgacaccg
     4441 acagcctttc tgtacaacaa ataattgctg tgcttctcta ctccagtaat gaatgtagtg
     4501 ccctgctgga tctgggacag caagactttt gagtccagat tcgccatccc atttcttgtt
     4561 ccctaacgtt tcctcagaag cgagcgagcc tcttccacaa tttggggaca ctgtggaaga
     4621 acttcttgct tggggagttc tgcagcagtc gaaggtacaa gaatataaaa actggtatct
     4681 cgacacagct cttacagcat attctcaaca caaggaacag taatgtgccg cagcaattcc
     4741 ttcggtggaa aaaactgact gtatccgcgt aagaggagta attctttttt gaattttatg
     4801 aggcttcaga gctgtatttc ggccaaccca gtctttgttc agtttctagc ggcttcaagg
     4861 ttgcccagtt ttacgccttc tcgctcaaaa cagacactag ttttctttga tagcctggtc
     4921 tcacattgac ttcatctaag cttattacat tcgtctcata aatcgtttgc tttttcccca
     4981 gccttagctc cagggaccag cctactgaac atcgaaaagg tcaggaacga gatcacggga
     5041 ctgagacggc gagccggcta gagcggatag agccgtactg ccgctctagt ttttcttttg
     5101 ctctctatgg ttaggagcgc aagcctctct tcggcccgga aagatttaag ttcgtgaatg
     5161 catacgcaag actcggaggt agttccaccg ccgagaccgc gtccgccccg cgagcacaga
     5221 gcctcgcctt tgccgatccg ccgcccgtcc acacccgccg ccagctcacc atgctgcgga
     5281 atctgctggt gaggagcttc tcatcttgca ttcatgatgg gggcgaggga gtggagagga
     5341 ggctgaggca ccacgacaga aaaccctcac ctgggagggg tgccgaaggc ggctaatgaa
     5401 gtcaccttga ttcgtgtcct ttttccctga ccttactttc ccttccgtct attcctctcg
     5461 ccccaggccg ttactgggta gatgtaagga gtggagaggc tggggaagtg accagagact
     5521 cgtgggttag acccgtcttg ggttcttagt caccacctct aaccctgtcg gtcattgatg
     5581 aggttagtag ctccgatctg agtaaaacca agacccaggt ggggagtgtc tgacacccgg
     5641 aagcatcaag cagaaaggtg tttttctggt actgaaaggt tttaagtttc aatggttttg
     5701 agctttgcga gcttgagaag gcagtttaaa aaccaagcca ttcaattttg ggtggaactt
     5761 taggaatagt catggctgaa gtgcagtgtt atttccgtca ggcgattgtg cattgtcctt
     5821 cacccctcca tcgactttga gcatgtgaaa tatttcccca aattttgata aagaaaaatg
     5881 cgacaatgat tattttattt tttaaattat ttaatgaagt tttgcttcat taaaatcctc
     5941 aatgtaattt tagaaaatcg agagcatatt tgtttcatta tttaattcaa ctggatatgt
     6001 agtgcatgtc tcttctttaa aagatgtata ccctctttaa aattttttag gaaaaatgta
     6061 atgagagaag cattttaatt gagaaggaat catgccagtc tatttgctgt caagtaatag
     6121 ggtttttttt gttgttgttg tttttattta ttttatttta ttttatttta ttttattttt
     6181 ttttttgaga cgtagtctca ctctgttgcc caggctggag tgcagtggca cgatctcggc
     6241 tcactgcaat ttccgtctcc aggttcaagc gattctgctg cctcagcctt ccaactagct
     6301 gggattacag gcgctcgcca ccaagcccta ctaatttttg tattattaat agagacgggg
     6361 tttcaccatg ttggccaggc tggtctcgga ctcgacctca agtgatccgc ccgcctcagc
     6421 ctcccaaagc gctgggatta ctggcataag ccactgtgca cggccttttt tattttttta
     6481 ttttttattt ttattttttg agcggagttt cattcttgtc gcccaggctg gagtgcagtg
     6541 gtgcaatctc ggctcactgc aaaccccgcc tcctggattc aagcgattct cccgcctcag
     6601 cctcctgagt agctagaatt tcaggtgcgc accaccatgc ccagctaatt tttgtgtttc
     6661 tagtagagac ggggtttcgc catgttggct aggccgggtc ttgaactcct tacctcaggt
     6721 gatccaccca ccttggcctc ccaaagtact gggattacag gcgtgagcca ccgcgcctgg
     6781 ccaagtaaca gatttttttt ttttgagatg gagtttcact ctttttgccc agtctgtagt
     6841 gcaatggggc gatcttggct caccacaacc tctgcctccc gggttcaagc gattctcctg
     6901 ccttagcctc ccgattagct gggattacag gcatgcacca ccatgcctgg ttaattttgt
     6961 atttttagta gagacagggt ttctccatgt tgttcaggct gttctcgaac tccggacttc
     7021 aagtgatctg cctgcctagt aatagaattt tttctttgaa acggagtttc acacttgttg
     7081 cccaggctgg agtgcaatgg cgcgatctca gctcactgca acctccgcct cccaggttca
     7141 agctattctc ctgcctcagc ctcccaagta tctgggatta caggcaagtg ccaccacgcc
     7201 tggctaattt tgtattttta gtagagatgg ggtttctaat gttggtcaga gtggtctcga
     7261 actcctgact tcaggtgatc cgcccccctc cccggcctcc caaagtgctg ggattacagg
     7321 tgtgagccac cgcgcctggc aggtttttga tgatatggca gtatattatg aaaatatttg
     7381 ggtttggtgt attttggtta agcactattt ttgtaatatg tgtatgtcaa tatgtttgca
     7441 tcaaggatta taaaatgcta ctggttctgt tcaggtagag atcatgattt tattttttgg
     7501 ttagaatcta tatgaattgc ttcttgaata tgttatttgg agactgcaga atgtaattcg
     7561 aagagcagag gatggataac aaattgaaaa acctggatgc tcttgcatga ctttgctgct
     7621 gacaaatcag atatgcttca agtctcaatt taccttttct ataatatgaa cgactgaaaa
     7681 tagttggttt tgaaggtttc tttaggctct aagtctattt aataatattt tcattttagg
     7741 ctcttcgtca gattgggcag aggacgataa gaacagcaag ccgcaggcat tttaaaaata
     7801 aagttccgga gaagcaaaaa ctgttccagg tactgaagta ttttatagga gatgttactt
     7861 gtaattatta gattaccaaa aggtaagagt tgggataaac aagtatgtgt ataaattaga
     7921 tcatatgaca atataaacat tacaaaaaaa gtcaaggaca tgtaccatag tgctaatagt
     7981 ggttgtctct tggggaaaga cctggtggag cagagcaatt tacctttata agtagtttga
     8041 ttatgagtga tttttgtttt attatggttt ctagcttttc ctaaataaag cttaaagtga
     8101 ttaaaaatag ttgtatcata tatacctaaa aatacaatac tttattgaac agatgtacta
     8161 aatagctact gtatgctgga ttagatgcta gggatataat ttagggttat aaaccaaaat
     8221 cccttcccca cttttttaag ggaaaaaaag tttcctatga ctaaatctaa tgtgtggcaa
     8281 ttattggttc ttttatattt aagttatatt aaagttgttg agatcagtgt gtatgtttta
     8341 tagctttaat gtttatatat tctttggaaa gaccgtcatt aatggatctc aaattaacgg
     8401 tgaaagaaga atgctgactt ttaggaattc ttattttaaa agtagtagta gtagagacgg
     8461 aataaagaaa attgtaggtc ttttaaattt taatacttaa aatactttaa ttctagggat
     8521 ttaaggagct atatcatttt atatgcctgt tcttttgaaa cttagttgga aatgagtgag
     8581 acatttatta ttgtcttttt tttttttttt taaatctagg aggatgatga aattccactg
     8641 tatctaaagg gtggggtagc tgatgccctc ctgtatagag ccaccatgat tcttacagtt
     8701 ggtggtaagg cctttggaat gtttgaaggt ttatagaaaa gtaatagtaa tgataactga
     8761 taactttatt gcttttgcta tgccagtatt ttaatgcagg cagtttgtct ccagaatttg
     8821 atatcttact atatctcata atgattcaag agggtgactg atacatactg tataagagca
     8881 ttgtgatgtc ttcttttttt tttgaaatgg agttttgctc ctgttgccca ggctggattg
     8941 caatggcgcg atctcggctc actgcaacct ctgcctcccg ggttcaagcg attctcctgc
     9001 ctcagcctcc cgagtagctg ggattacagg cgtgcaccac catgcctggc taattttgta
     9061 gttttagtag agacggagtt tctccgtgtt ggtcaggctg gtcttgaact cctgacctca
     9121 ggtgatctgc ccgccacagc ctcccaaagt attgggatta caggcatgag ccactgcgcc
     9181 tggccttgat gtcttaataa aaaaataaca ccagtaagat ttgatgatta tgttgtgata
     9241 gagttggctt tctgagtccc attgatggca gtgaaggcta tatttcagtc aaaaacatcc
     9301 attatatccc acctcttaca gcaagggctt taaaacattt tgactgtgat gcaaaataaa
     9361 agcacacttt acatctaaac tttgtacaga catatttatg accaaaacaa aatttgtaca
     9421 gaacaatact tccactggaa ggtaaagaga agggatgcta tatttgagtg attattttat
     9481 gcctatcata ttcataggaa tgccacatac ttggatttct gtgagtcttg catgaagtct
     9541 ataatatgtc ctcattttaa agatgaagaa atcatattaa ttacctgttt gggaaactct
     9601 actctcacac cacaacaatc aacatggaag atctctgttg ccaaatgtgt gggggttttt
     9661 ccctgcacac caagtaatgg acaccagcta ggtgtcctcc agttcagttt ccacactatc
     9721 tactgggaga tagcatcaga ttccacaggt tgatggcaca agactgtccc ctccttccca
     9781 ccagttgcaa gtttgggtct tcggaacttc tgactgactg gctttaagtt ggggttccca
     9841 caatctcctc gttggggaaa cacatttacc agtttattat aaaaggtata ggctgggcat
     9901 ggtggctcat gcctgtaatc ccagcacttt gggaggctga ggctggcaga tcacctgagg
     9961 tcaggagttc aagactagcc tggcacacat ggtgaaaccc tgtctgtact aaaaacacaa
    10021 aaatgagtcg ggtgtgatgg cacacacttg caatcccagc tactcgtgaa gctgaggcag
    10081 gaagattgct tgaactggta ggcggaggtt gcagtgagct cagattgtgt cactgcactc
    10141 cagcctgggc gacagaggga gactccatct caaaaaaaag aaaaaaaaag ataaagccct
    10201 aaattccttt tggccaatgt gacatttctt agacattctg gtaagtagtt tttaaaatga
    10261 aattccagtt atcagctttt tttttttttt tttttttttt gagatggagt ttcgctcttg
    10321 ttgcccaggc tggagtgcaa tggcgcgatc tcggcttact gcaacctctg cctcccaggt
    10381 tcaagcaatt ctcctgcctc agcctcccaa gtagctggga ttacaggcat gcaccaccac
    10441 gcctggctaa ttttgtattt ttagtagaga cagggtttct ccatgttgag gctggtctcg
    10501 aactcctgac ctcaggtgat ccgcccgcct cggcctccca aagtgctggg attacaggtt
    10561 tcagcaacta tgcccggccc agttatcagc tattttttat attacatttg attactttaa
    10621 aattatatca ctcttttttt ttacattcac tttagggaag gcttgcatat tttgaactct
    10681 ttttttcttt ggcatttaat catccttagc atttctcagg cagtcttggg ttaatatgga
    10741 aataaccttt gtatacgttc ttaatcttat ttttaaaaat ttttgcattt tatgttgaca
    10801 aatatattat tgtgccacac tttctatatt aaaactagat taaatatatt ttacatatat
    10861 ttatttaaaa tttttccagc acctatcaaa atactttttt gtgttccaat taaggtagtc
    10921 ttaatgcaaa cttacaactt ttgaactgga atattaaatt tatacactta gatcttagaa
    10981 tttatggtta agttcttttt tgttttttat ttttaggaac agcatatgcc atatatgagc
    11041 tggctgtggc ttcatttccc aagaagcagg agtgacttca gtcatcccag caatcgcttg
    11101 gttcagtttc attcagctct ctatggacca gtaatctgat aaataaccga gctcttcttt
    11161 ggggatcaat atttattgac ttgtagtaac tgccaccaat aaagcagtct ttaccatgct
    11221 ttgtctgatt ttatcacttt atgccaataa tttcaacttt tggtcagcct tgtaaatctt
    11281 aggtaaggat caaagaaatg attcttttgc cactaaactt gtgaaagcaa cattattgtg
    11341 atgttatttt agtgaaattt tacttctaac agctcaaaat tagtctcaac ttctgtctca
    11401 acttgccgtt tgaatgcata ttgctttttc cccctaccca ctccagtata tcttggaata
    11461 tgctagaacc cgggggtagt ggaagcagat tatctttggc tcctggggtc tatatttctt
    11521 tttttttttt tttttctcag ctatgtgaga gtatgcttaa atattggata tatcccacag
    11581 cttagaatta ggctttagta gaatggcttt gtaagatatt tcagtatttg tttttttcta
    11641 cactccgagt gggttcaggt aggtttctct catcaggaac caaccaaagc aggacagttc
    11701 ctgggctccc cagctatcat ctaatggacc cacaactgtt taacctaaac agtagttagg
    11761 tgcttaataa atggtagcca taataggctg ctcataatag gaatgtttca aaaaggtcta
    11821 tttagggaac acaaaatcca aatagataca gagagtaaga atcaaaaaga attacgatgc
    11881 tgctctaggt agtttgcttc agggtttgca gaacccttcc tgctttgaga gaactatgca
    11941 tgaagctgga aatttcatag acttcaagtt tgggactccc ttttcctggg tcctgttgtg
    12001 catccatgtg gagagtagca tcttgtgaac cacttcagga ctttccttct atatatatcg
    12061 agattggtaa ttcgtcaaga gatactgagt gcctactgtg tcctaactgg ttttaggtac
    12121 ctaggaatca ctggtgaaca aaagcagatt cttcccctca tatgttatag aaaggggtgg
    12181 taggaaagag agtttaaaaa taaattgcac agtaggttaa aagataagtt ctatggaaaa
    12241 agaccaaagg ggagaactca catgggtgtg gttggttggt tgcagtatta aagttagggt
    12301 aggcttcaac aggaaggtca gattgtttaa accaagcggc gactgagatg tcctaaatgc
    12361 acagcgacgg attcgcgcta tttagaaaga gagagcaata tttcaagaat gcatgcgtca
    12421 attttacgca gactatcttt ctagggttaa tctagctgca tcaggatcat atcgtcgggt
    12481 cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg gaggaagaag
    12541 cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga gtttgccgag gatgactgct
    12601 gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa ggtgtggcca
    12661 tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggataccagt tcgtcgcggc
    12721 ttttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg aacaataccg
    12781 gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggtgcg gcgctgggat
    12841 attacgtcag cgaggacggg tatcctggct ggatgccgca gaaatggaca tggatacccc
    12901 gtgagttacc cggcgggcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
    12961 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    13021 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    13081 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    13141 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    13201 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    13261 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    13321 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    13381 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    13441 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    13501 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    13561 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    13621 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    13681 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    13741 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
    13801 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    13861 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    13921 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    13981 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    14041 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    14101 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    14161 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    14221 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    14281 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    14341 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    14401 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    14461 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    14521 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    14581 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    14641 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    14701 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    14761 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    14821 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    14881 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    14941 gaaatgttga atactcat
//
