LOCUS       Exported               13758 bp ds-DNA     circular SYN 05-SEP-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13758)
  AUTHORS   The Weissman lab
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 8, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13758
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    874..936
                     /label=5'ITR
     misc_feature    complement(1400..1993)
                     /label=WPRE
                     /label=WPRE; Woodchuck post-transcriptional
     primer_bind     complement(1893..1912)
                     /label=HKO083
     CDS             complement(2016..2615)
                     /label=Puro (-)
     promoter        complement(2628..3130)
                     /label=PGK (-)
     misc_feature    3137..11030
                     /label=COX7A2
     misc_feature    4187..5186
                     /label=Promoter 1000bp upstream
     misc_feature    5187..5253
                     /label=5' UTR
     misc_feature    5254..5271
                     /label=Exon1
     misc_feature    5254..5271
                     /label=MTS part 1
     misc_feature    7709..7798
                     /label=Exon2
     misc_feature    7709..7759
                     /label=MTSpart2
     misc_feature    7741..7749
                     /label=mutations
     misc_feature    8584..8668
                     /label=Exon 3
     misc_feature    10972..11030
                     /label=Exon 4
     misc_feature    11028..11030
                     /label=stop codon
     misc_feature    11031..11124
                     /label=ACTB-3'UTR
     misc_feature    11212..11246
                     /label=3'ITR
     primer_bind     complement(11737..11753)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11761..11777
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11785..11815)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11830..11851
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(12139..12727)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12898..13758)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc agggtttatt acagggacag cagagatcca
     1381 gtttggttaa ttagctagcg atgcggggag gcggcccaaa gggagatccg actcgtctga
     1441 gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg
     1501 tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg
     1561 gcaacacagg cgagcagcca aggaaaggac gatgatttcc ccgacaacac cacggaattg
     1621 tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc
     1681 gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca
     1741 atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg
     1801 acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa
     1861 atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat
     1921 agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc
     1981 agaggttgat taccgataag cttgatatcg aattcttaac tggcaccggg cttgcgggtc
     2041 atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc
     2101 cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg
     2161 cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg
     2221 tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc
     2281 aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc
     2341 gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc
     2401 acggcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg
     2461 aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc
     2521 gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg
     2581 cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg tggtaccccc tggggagaga
     2641 ggtcggtgat tcggtcaacg agggagccga ctgccgacgt gcgctccgga ggcttgcaga
     2701 atgcggaaca ccgcgcgggc aggaacaggg cccacactac cgccccacac cccgcctccc
     2761 gcaccgcccc ttcccggccg ctgctctcgg cgcgccctgc tgagcagccg ctattggcca
     2821 cagcccatcg cggtcggcgc gctgccattg ctccctggcg ctgtccgtct gcgagggtac
     2881 tagtgagacg tgcggcttcc gtttgtcacg tccggcacgc cgcgaaccgc aaggaacctt
     2941 cccgacttag gggcggagca ggaagcgtcg ccggggggcc cacaagggta gcggcgaaga
     3001 tccgggtgac gctgcgaacg gacgtgaaga atgtgcgaga cccagggtcg gcgccgctgc
     3061 gtttcccgga accacgccca gagcagccgc gtccctgcgc aaacccaggg ctgccttgga
     3121 aaaggcgcaa acgcgttggt gagggacgga ttaactatgt taaatgctgc taatagatgg
     3181 agaaggtgaa aattgagatt gacagtttat ttagcaacct ggaagctatt ggtgattcta
     3241 aacaagagca gtaaaagcct gcccaaaatg ggatcaaaaa aagattagga ggaggagaag
     3301 gagagacaag tataaagaaa tctataggat ggtaacggta atcaaaggag tttgttcgtt
     3361 tgtttttaat ttgggaatgt gttaatagat ggaatgatcc agtaaagggg gaaattgtac
     3421 tgaataacat gcatacaaga atctatgtct actttaggat ctatttaatg tgaattcatg
     3481 gctttttttt tttttttgat atgaagtctc actctgtcgc ccaggctgga gtgcagtgga
     3541 gcgatcttgg ctcactgcaa gctccacctt ctgggttcac gccattctcc tgcctcaacc
     3601 tcctgagtag ctgggactac aggcgcccgc caccacgccc gccttatttt ttgtattttt
     3661 agtagagaca gggtttcacg gctttagcca agatggtctg gatctcctga cctcatgatc
     3721 cgcccgcctc ggcctcccaa agtactggga ttacaggcgt gagccactgc gcctggcctc
     3781 atggctcttt ttggacaggg tcataagctt cttagatctg tcacccaatg taattaattg
     3841 atgtattggt taatactgtc tttagcagtg ctaggggatt ggtaagaaat ccacatcatc
     3901 tctgcgcttg ggaaatttac aatctagagt tggctaagtg gagaaaacat aagagagcac
     3961 atacaactaa caaattcaga ttatggacaa tgtacttaaa aaacccccac gccctagcta
     4021 ttggcacgtg gtaattttgc ccagagtttt ggttatatgc ataatccaga ggggtgtaga
     4081 aaaccaggtc ctttcagtac caatctaagg aagacatgaa ttggggtgtt tataaacctt
     4141 ggtgaggaag agaattttaa ctgggactgg taacttggtg ggcccattga catacagtag
     4201 attgtgaaga agaaaacccc aaggattata aggaatagtt ttaatattac taatttttat
     4261 cctttgctga aactatgggt ttcttatttg aaatggcaat caaataattt gtaagagttt
     4321 gtagaaagtg gaaagattat tgtaatacag ggtagatatt tgcatttgta catacttgag
     4381 ggtaagtaca agaaataaac acttgtaatg gggattactt aagcaaaatg gatgacaccg
     4441 acagcctttc tgtacaacaa ataattgctg tgcttctcta ctccagtaat gaatgtagtg
     4501 ccctgctgga tctgggacag caagactttt gagtccagat tcgccatccc atttcttgtt
     4561 ccctaacgtt tcctcagaag cgagcgagcc tcttccacaa tttggggaca ctgtggaaga
     4621 acttcttgct tggggagttc tgcagcagtc gaaggtacaa gaatataaaa actggtatct
     4681 cgacacagct cttacagcat attctcaaca caaggaacag taatgtgccg cagcaattcc
     4741 ttcggtggaa aaaactgact gtatccgcgt aagaggagta attctttttt gaattttatg
     4801 aggcttcaga gctgtatttc ggccaaccca gtctttgttc agtttctagc ggcttcaagg
     4861 ttgcccagtt ttacgccttc tcgctcaaaa cagacactag ttttctttga tagcctggtc
     4921 tcacattgac ttcatctaag cttattacat tcgtctcata aatcgtttgc tttttcccca
     4981 gccttagctc cagggaccag cctactgaac atcgaaaagg tcaggaacga gatcacggga
     5041 ctgagacggc gagccggcta gagcggatag agccgtactg ccgctctagt ttttcttttg
     5101 ctctctatgg ttaggagcgc aagcctctct tcggcccgga aagatttaag ttcgtgaatg
     5161 catacgcaag actcggaggt agttccggtt ccggcgtggc cattttcgtt ggtggtgttc
     5221 agttgtggcg gttgctggtc agtaacagcc aagatgctgc ggaatctgct ggtgaggagc
     5281 ttctcatctt gcattcatga tgggggcgag ggagtggaga ggaggctgag gcaccacgac
     5341 agaaaaccct cacctgggag gggtgccgaa ggcggctaat gaagtcacct tgattcgtgt
     5401 cctttttccc tgaccttact ttcccttccg tctattcctc tcgccccagg ccgttactgg
     5461 gtagatgtaa ggagtggaga ggcaggggaa gtgaccagag actcgtgggt tagacccgtc
     5521 ttgggttctt agtcaccacc tctaaccctg tcggtcattg atgaggttag tagctccgat
     5581 ctgagtaaaa ccaagaccca ggtggggagt gtctgacacc cggaagcatc aagcagaaag
     5641 gtgtttttct ggtactgaaa ggttttaagt ttcaatggtt ttgagctttg cgagcttgag
     5701 aaggcagttt aaaaaccaag ccattcaatt ttgggtggaa ctttaggaat agtcatggct
     5761 gaagtgcagt gttatttccg tcaggcgatt gtgcattgtc cttcacccct ccatcgactt
     5821 tgagcatgtg aaatatttcc ccaaattttg ataaagaaaa atgcgacaat gattatttta
     5881 ttttttaaat tatttaatga agttttgctt cattaaaatc ctcaatgtaa ttttagaaaa
     5941 tcgagagcat atttgtttca ttatttaatt caactggata tgtagtgcat gtctcttctt
     6001 taaaagttgt ataccctctt taaaattttt taggaaaaat gtaatgagag aagcatttta
     6061 attgagaagg aatcatgcca gtctatttgc tgtcaagtaa tagggttttt tttgttgttg
     6121 ttgtttttat ttattttatt ttattttatt ttttgagacg tagtctcact ctgttgccca
     6181 ggctggagtg cagtggcacg atctcggctc actgcaattt ccgtctccag gttcaagcga
     6241 ttctgctgcc tcagccttcc aactagctgg gattacaggc gctcgccacc aagccctact
     6301 aatttttgta ttattaatag agacggggtt tcaccatgtt ggccaggctg gtctcggact
     6361 cttgacctca agtgatccgc ccgcctcagc ctcccaaagc gctgggatta ctggcataag
     6421 ccactgtgca cggccttttt tattttttta ttttttattt ttattttttg agcggagttt
     6481 cattcttgtc gcccaggctg gagtgcagtg gtgcaatctt ggctcactgc aaaccccgcc
     6541 tcctggattc aagcgattct cccgcctcag cctcctgagt agctagaatt tcaggtgcgc
     6601 accaccatgc ccagctaatt tttgtgtttc tagtagagac ggggtttcgc catgttggct
     6661 aggccgggtc ttgaactcct tacctcaggt gatccaccca ccttggcctc ccaaagtact
     6721 gggattacag gcgtgagcca ccgcgcctgg ccaagtaaca gatttttttt tttgagatgg
     6781 agtttcactc tttttgccca gtctgtagtg caatggggcg atcttggctc accacaacct
     6841 ctgcctcccg ggttcaagcg attctcctgc cttagcctcc cgattagctg ggattacagg
     6901 catgcaccac catgcctggt taattttgta tttttagtag agacagggtt tctccatgtt
     6961 gttcaggctg gtctcgaact ccggacttca agtgatctgc ctgcctagta atagaatttt
     7021 ttctttgaaa cggagtttca cacttgttgc ccaggctgga gtgcaatggc gcgatctcag
     7081 ctcactgcaa cctccgcctc ccaggttcaa gctattctcc tgcctcagcc tcccaagtat
     7141 ctgggattac aggcaagtgc caccacgcct ggctaatttt gtatttttag tagagatggg
     7201 gtttctaatg ttggtcagag tggtctcgaa ctcctgactt caggtgatcc gcccccctcc
     7261 ccggcctccc aaagtgctgg gattacaggt gtgagccacc gcgcctggca ggtttttgat
     7321 gatatggcag tatattatga aaatatttgg gtttggtgta ttttggttaa gcactatttt
     7381 tgtaatatgt gtatgtcaat atgtttgcat caaggattat aaaatgctac tggttctgtt
     7441 caggtagaga tcatgatttt attttttggt tagaatctat atgaattgct tcttgaatat
     7501 gttatttgga gactgcagaa tgtaattcga agagcagagg atggataaca aattgaaaaa
     7561 cctggatgct cttgcatgac tttgctgctg acaaatcaga tatgcttcaa gtctcaattt
     7621 accttttcta taatatgaac gactgaaaat agttggtttt gaaggtttct ttaggctcta
     7681 agtctattta ataatatttt cattttaggc tcttcgtcag attgggcaga ggacgataag
     7741 aacagcaagc cgcaggcatt ttaaaaataa agttccggag aagcaaaaac tgttccaggt
     7801 actgaagtat tttataggag atgttacttg taattattag attaccaaaa ggtaagagtt
     7861 gggataaaca agtatgtgta taaattagat catatgacaa tataaacatt acaaaaaaag
     7921 tcaaggacat gtaccatagt gctaatagtg gttgtctctt ggggaaagac ctggtggagc
     7981 agagcaattt acctttataa gtagtttgat tatgagtgat ttttgtttta ttatggtttc
     8041 tagcttttcc taaataaagc ttaaagtgat taaaaatagt tgtatcatat atacctaaaa
     8101 tacaatactt tattgaacag atgtactaaa tagctactgt atgctggatt agatgctagg
     8161 gatataattt agggttataa accaaaatcc cttccccact tttttaaggg aaaaaagttt
     8221 cctatgacta aatctaatgt gtggcaatta ttggttcttt tatatttaag ttatattaaa
     8281 gttgttgaga tcagtgtgta tgttttatag ctttaatgtt tatatattct ttggaaagac
     8341 cgtcattaat ggatctcaaa ttaacggtga aagaagaatg ctgactttta ggaattctta
     8401 ttttaaaagt agtagtagta gagacagaat aaagaaaatt gtaggtcttt taaattttaa
     8461 tacttaaaat actttaattc tagggattta aggagctata tcattttata tgcctgttct
     8521 tttgaaactt agttggaaat gagtgagaca tttattattg tctttttttt tttttaaatc
     8581 taggaggatg atgaaattcc actgtatcta aagggtgggg tagctgatgc cctcctgtat
     8641 agagccacca tgattcttac agttggtggt aaggcctttg gaatgtttga aggtttatag
     8701 aaaagtaata gtaatgataa ctgataactt tattgctttt gctatgccag tattttaatg
     8761 caggcagttt gtctccagaa tttgatatct tactatatct cataatgatt caagagggtg
     8821 actgatacat actgtataag agcattgtga tgtcttcttt tttttgaaat ggagttttgc
     8881 tcctgttgcc caggctggat tgcaatggcg cgatctcggc tcactgcaac ctctgcctcc
     8941 cgggttcaag cgattctcct gcctcagcct cccgagtagc tgggattaca ggcgtgcacc
     9001 accatgcctg gctaattttg tagttttagt agagacggag tttctccgtg ttggtcaggc
     9061 tggtcttgaa ctcctgacct caggtgatct gcccgccaca gcctcccaaa gtattgggat
     9121 tacaggcatg agccactgcg cctggccttg atgtcttaat aaaaaaataa caccagtaag
     9181 atttgatgat tatgttgtga tagagttggc tttctgagtc ccattgatgg cagtgaaggc
     9241 tatatttcag tcaaaaacat ccattatatc ccacctctta cagcaagggc tttaaaacat
     9301 tttgactgtg atgcaaaata aaagcacact ttacatctaa actttgtaca gacatattta
     9361 tgaccaaaac aaaatttgta cagaacaata cttccactgg aaggtaaaga gaagggatgc
     9421 tatatttgag tgattatttt atgcctatca tattcatagg aatgccacat acttggattt
     9481 ctgtgagtct tgcatgaagt ctataatatg tcctcatttt aaagatgaag aaatcatatt
     9541 aattacctgt ttgggaaact ctactctcac accacaacaa tcaacatgga agatctctgt
     9601 tgccaaatgt gtgggggttt ttccctgcac accaagtaat ggacaccagc taggtgtcct
     9661 ccagttcagt ttccacacta tctactggga gatagcatca gattccacag gttgatggca
     9721 caagactgtc ccctccttcc caccagttgc aagtttgggt cttcggaact tctgactgac
     9781 tggctttaag ttggggttcc cacaatctcc tcgttgggga aacacattta ccagtttatt
     9841 ataaaaggta taggctgggc atggtggctc atgcctgtaa tcccagcact ttgggaggct
     9901 gaggctggca gatcacctga ggtcaggagt tcaagactag cctggcacac atggtgaaac
     9961 cctgtctgta ctaaaaacac aaaaatgagt cgggtgtgat ggcacacact tgcaatccca
    10021 gctactcgtg aagctgaggc aggaagattg cttgaactgg taggcggagg ttgcagtgag
    10081 ctcagattgt gtcactgcac tccagcctgg gcgacagagg gagactccat ctcaaaaaaa
    10141 agaaaaaaaa agataaagcc ctaaattcct tttggccaat gtgacatttc ttagacattc
    10201 tggtaagtag tttttaaaat gaaattccag ttatcagctt tttttttttt tttttgagat
    10261 ggagtttcgc tcttgttgcc caggctggag tgcaatggcg cgatctcggc ttactgcaac
    10321 ctctgcctcc caggttcaag taattctcct gcctcagcct cccaagtagc tgggattaca
    10381 ggcatgcacc accacgcctg gctaattttg tatttttagt agagacaggg tttctccatg
    10441 ttgaggctgg tctcgaactc ctgacctcag gtgatccgcc cgcctcggcc tcccaaagtg
    10501 ctgggattac aggtttcagc aactatgccc ggcccagtta tcagctattt tttatattac
    10561 atttgattac tttaaaatta tatcactctt tttttttaca ttcactttag ggaaggcttg
    10621 catattttga actatttttt tctttggcat ttaatcatcc ttagcatttc tcaggcagtc
    10681 ttgggttaat atggaaataa cctttgtata cgttcttaat cttattttta aaaatttttg
    10741 cattttatgt tgacaaatat attattgtgc cacactttct atattaaaac tagattaaat
    10801 atattttaca tatatttatt taaaattttt ccagcaccta tcaaaatact tttttgtgtt
    10861 ccaattaagg tagtcttaat gcaaacttac aacttttgaa ctggaatatt aaatttatac
    10921 acttagatct tagaatttat ggttaagttc ttttttgttt tttattttta ggaacagcat
    10981 atgccatata tgagctggct gtggcttcat ttcccaagaa gcaggagtga cttttggtct
    11041 ccctgggagt gggtggaggc agccagggct tacctgtaca ctgacttgag accagttgaa
    11101 taaaagtgca caccttaaaa atgagtttaa accaagcggt gactgagatg tcctaaatgc
    11161 acagcgacgg attcgcgcta tttagaaaga gagagcaata tttcaagaat gcatgcgtca
    11221 attttacgca gactatcttt ctagggttaa tctagctgca tcaggatcat atcgtcgggt
    11281 cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg gaggaagaag
    11341 cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga gtttgccgag gatgactgct
    11401 gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa ggtgtggcca
    11461 tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggataccagt tcgtcgcggc
    11521 ttttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg aacaataccg
    11581 gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggtgcg gcgctgggat
    11641 attacgtcag cgaggacggg tatcctggct ggatgccgca gaaatggaca tggatacccc
    11701 gtgagttacc cggcgggcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
    11761 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    11821 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    11881 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    11941 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    12001 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    12061 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    12121 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    12181 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    12241 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    12301 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    12361 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    12421 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    12481 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    12541 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
    12601 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    12661 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    12721 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    12781 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    12841 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    12901 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    12961 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    13021 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    13081 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    13141 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    13201 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    13261 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    13321 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    13381 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    13441 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    13501 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    13561 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    13621 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    13681 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    13741 gaaatgttga atactcat
//
