LOCUS       Exported               14941 bp ds-DNA     circular SYN 22-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14941)
  AUTHORS   The Weissman lab
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 6, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14941
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    874..936
                     /label=5'ITR
     misc_feature    complement(1400..1993)
                     /label=WPRE
                     /label=WPRE; Woodchuck post-transcriptional
     primer_bind     complement(1893..1912)
                     /label=HKO083
     CDS             complement(2016..2615)
                     /label=Puro (-)
     promoter        complement(2628..3130)
                     /label=PGK (-)
     misc_feature    3137..12307
                     /label=COX7A2
     misc_feature    4187..5186
                     /label=Promoter 1000bp upstream
     misc_feature    5187..5253
                     /label=5' UTR
     misc_feature    5254..5271
                     /label=Exon1
     misc_feature    5254..5271
                     /label=MTS part 1
     misc_feature    7723..7812
                     /label=Exon2
     misc_feature    7723..7773
                     /label=MTSpart2
     misc_feature    7755..7763
                     /label=mutations
     misc_feature    8603..8687
                     /label=Exon 3
     misc_feature    11000..11058
                     /label=Exon 4
     misc_feature    11059..11201
                     /label=3' UTR
     misc_feature    11202..12201
                     /label=1000 bp downstream
     misc_feature    12322
                     /label=extra sequence in pJL242-3
     misc_feature    12395..12429
                     /label=3'ITR
     primer_bind     complement(12920..12936)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12944..12960
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12968..12998)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13013..13034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(13322..13910)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(14081..14941)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc agggtttatt acagggacag cagagatcca
     1381 gtttggttaa ttagctagcg atgcggggag gcggcccaaa gggagatccg actcgtctga
     1441 gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg
     1501 tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg
     1561 gcaacacagg cgagcagcca aggaaaggac gatgatttcc ccgacaacac cacggaattg
     1621 tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc
     1681 gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca
     1741 atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg
     1801 acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa
     1861 atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat
     1921 agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc
     1981 agaggttgat taccgataag cttgatatcg aattcttaac tggcaccggg cttgcgggtc
     2041 atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc
     2101 cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg
     2161 cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg
     2221 tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc
     2281 aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc
     2341 gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc
     2401 acggcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg
     2461 aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc
     2521 gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg
     2581 cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg tggtaccccc tggggagaga
     2641 ggtcggtgat tcggtcaacg agggagccga ctgccgacgt gcgctccgga ggcttgcaga
     2701 atgcggaaca ccgcgcgggc aggaacaggg cccacactac cgccccacac cccgcctccc
     2761 gcaccgcccc ttcccggccg ctgctctcgg cgcgccctgc tgagcagccg ctattggcca
     2821 cagcccatcg cggtcggcgc gctgccattg ctccctggcg ctgtccgtct gcgagggtac
     2881 tagtgagacg tgcggcttcc gtttgtcacg tccggcacgc cgcgaaccgc aaggaacctt
     2941 cccgacttag gggcggagca ggaagcgtcg ccggggggcc cacaagggta gcggcgaaga
     3001 tccgggtgac gctgcgaacg gacgtgaaga atgtgcgaga cccagggtcg gcgccgctgc
     3061 gtttcccgga accacgccca gagcagccgc gtccctgcgc aaacccaggg ctgccttgga
     3121 aaaggcgcaa acgcgttggt gagggacgga ttaactatgt taaatgctgc taatagatgg
     3181 agaaggtgaa aattgagatt gacagtttat ttagcaacct ggaagctatt ggtgattcta
     3241 aacaagagca gtaaaagcct gcccaaaatg ggatcaaaaa aagattagga ggaggagaag
     3301 gagagacaag tataaagaaa tctataggat ggtaacggta atcaaaggag tttgttcgtt
     3361 tgtttttaat ttgggaatgt gttaatagat ggaatgatcc agtaaagggg gaaattgtac
     3421 tgaataacat gcatacaaga atctatgtct actttaggat ctatttaatg tgaattcatg
     3481 gctttttttt tttttttgat atgaagtctc actctgtcgc ccaggctgga gtgcagtgga
     3541 gcgatcttgg ctcactgcaa gctccacctt ctgggttcac gccattctcc tgcctcaacc
     3601 tcctgagtag ctgggactac aggcgcccgc caccacgccc gccttatttt ttgtattttt
     3661 agtagagaca gggtttcacg gctttagcca agatggtctg gatctcctga cctcatgatc
     3721 cgcccgcctc ggcctcccaa agtactggga ttacaggcgt gagccactgc gcctggcctc
     3781 atggctcttt ttggacaggg tcataagctt cttagatctg tcacccaatg taattaattg
     3841 atgtattggt taatactgtc tttagcagtg ctaggggatt ggtaagaaat ccacatcatc
     3901 tctgcgcttg ggaaatttac aatctagagt tggctaagtg gagaaaacat aagagagcac
     3961 atacaactaa caaattcaga ttatggacaa tgtacttaaa aaacccccac gccctagcta
     4021 ttggcacgtg gtaattttgc ccagagtttt ggttatatgc ataatccaga ggggtgtaga
     4081 aaaccaggtc ctttcagtac caatctaagg aagacatgaa ttggggtgtt tataaacctt
     4141 ggtgaggaag agaattttaa ctgggactgg taacttggtg ggcccattga catacagtag
     4201 attgtgaaga agaaaacccc aaggattata aggaatagtt ttaatattac taatttttat
     4261 cctttgctga aactatgggt ttcttatttg aaatggcaat caaataattt gtaagagttt
     4321 gtagaaagtg gaaagattat tgtaatacag ggtagatatt tgcatttgta catacttgag
     4381 ggtaagtaca agaaataaac acttgtaatg gggattactt aagcaaaatg gatgacaccg
     4441 acagcctttc tgtacaacaa ataattgctg tgcttctcta ctccagtaat gaatgtagtg
     4501 ccctgctgga tctgggacag caagactttt gagtccagat tcgccatccc atttcttgtt
     4561 ccctaacgtt tcctcagaag cgagcgagcc tcttccacaa tttggggaca ctgtggaaga
     4621 acttcttgct tggggagttc tgcagcagtc gaaggtacaa gaatataaaa actggtatct
     4681 cgacacagct cttacagcat attctcaaca caaggaacag taatgtgccg cagcaattcc
     4741 ttcggtggaa aaaactgact gtatccgcgt aagaggagta attctttttt gaattttatg
     4801 aggcttcaga gctgtatttc ggccaaccca gtctttgttc agtttctagc ggcttcaagg
     4861 ttgcccagtt ttacgccttc tcgctcaaaa cagacactag ttttctttga tagcctggtc
     4921 tcacattgac ttcatctaag cttattacat tcgtctcata aatcgtttgc tttttcccca
     4981 gccttagctc cagggaccag cctactgaac atcgaaaagg tcaggaacga gatcacggga
     5041 ctgagacggc gagccggcta gagcggatag agccgtactg ccgctctagt ttttcttttg
     5101 ctctctatgg ttaggagcgc aagcctctct tcggcccgga aagatttaag ttcgtgaatg
     5161 catacgcaag actcggaggt agttccggtt ccggcgtggc cattttcgtt ggtggtgttc
     5221 agttgtggcg gttgctggtc agtaacagcc aagatgctgc ggaatctgct ggtgaggagc
     5281 ttctcatctt gcattcatga tgggggcgag ggagtggaga ggaggctgag gcaccacgac
     5341 agaaaaccct cacctgggag gggtgccgaa ggcggctaat gaagtcacct tgattcgtgt
     5401 cctttttccc tgaccttact ttcccttccg tctattcctc tcgccccagg ccgttactgg
     5461 gtagatgtaa ggagtggaga ggctggggaa gtgaccagag actcgtgggt tagacccgtc
     5521 ttgggttctt agtcaccacc tctaaccctg tcggtcattg atgaggttag tagctccgat
     5581 ctgagtaaaa ccaagaccca ggtggggagt gtctgacacc cggaagcatc aagcagaaag
     5641 gtgtttttct ggtactgaaa ggttttaagt ttcaatggtt ttgagctttg cgagcttgag
     5701 aaggcagttt aaaaaccaag ccattcaatt ttgggtggaa ctttaggaat agtcatggct
     5761 gaagtgcagt gttatttccg tcaggcgatt gtgcattgtc cttcacccct ccatcgactt
     5821 tgagcatgtg aaatatttcc ccaaattttg ataaagaaaa atgcgacaat gattatttta
     5881 ttttttaaat tatttaatga agttttgctt cattaaaatc ctcaatgtaa ttttagaaaa
     5941 tcgagagcat atttgtttca ttatttaatt caactggata tgtagtgcat gtctcttctt
     6001 taaaagatgt ataccctctt taaaattttt taggaaaaat gtaatgagag aagcatttta
     6061 attgagaagg aatcatgcca gtctatttgc tgtcaagtaa tagggttttt tttgttgttg
     6121 ttgtttttat ttattttatt ttattttatt ttattttatt tttttttttg agacgtagtc
     6181 tcactctgtt gcccaggctg gagtgcagtg gcacgatctc ggctcactgc aatttccgtc
     6241 tccaggttca agcgattctg ctgcctcagc cttccaacta gctgggatta caggcgctcg
     6301 ccaccaagcc ctactaattt ttgtattatt aatagagacg gggtttcacc atgttggcca
     6361 ggctggtctc ggactcgacc tcaagtgatc cgcccgcctc agcctcccaa agcgctggga
     6421 ttactggcat aagccactgt gcacggcctt ttttattttt ttatttttta tttttatttt
     6481 ttgagcggag tttcattctt gtcgcccagg ctggagtgca gtggtgcaat ctcggctcac
     6541 tgcaaacccc gcctcctgga ttcaagcgat tctcccgcct cagcctcctg agtagctaga
     6601 atttcaggtg cgcaccacca tgcccagcta atttttgtgt ttctagtaga gacggggttt
     6661 cgccatgttg gctaggccgg gtcttgaact ccttacctca ggtgatccac ccaccttggc
     6721 ctcccaaagt actgggatta caggcgtgag ccaccgcgcc tggccaagta acagattttt
     6781 tttttttgag atggagtttc actctttttg cccagtctgt agtgcaatgg ggcgatcttg
     6841 gctcaccaca acctctgcct cccgggttca agcgattctc ctgccttagc ctcccgatta
     6901 gctgggatta caggcatgca ccaccatgcc tggttaattt tgtattttta gtagagacag
     6961 ggtttctcca tgttgttcag gctgttctcg aactccggac ttcaagtgat ctgcctgcct
     7021 agtaatagaa ttttttcttt gaaacggagt ttcacacttg ttgcccaggc tggagtgcaa
     7081 tggcgcgatc tcagctcact gcaacctccg cctcccaggt tcaagctatt ctcctgcctc
     7141 agcctcccaa gtatctggga ttacaggcaa gtgccaccac gcctggctaa ttttgtattt
     7201 ttagtagaga tggggtttct aatgttggtc agagtggtct cgaactcctg acttcaggtg
     7261 atccgccccc ctccccggcc tcccaaagtg ctgggattac aggtgtgagc caccgcgcct
     7321 ggcaggtttt tgatgatatg gcagtatatt atgaaaatat ttgggtttgg tgtattttgg
     7381 ttaagcacta tttttgtaat atgtgtatgt caatatgttt gcatcaagga ttataaaatg
     7441 ctactggttc tgttcaggta gagatcatga ttttattttt tggttagaat ctatatgaat
     7501 tgcttcttga atatgttatt tggagactgc agaatgtaat tcgaagagca gaggatggat
     7561 aacaaattga aaaacctgga tgctcttgca tgactttgct gctgacaaat cagatatgct
     7621 tcaagtctca atttaccttt tctataatat gaacgactga aaatagttgg ttttgaaggt
     7681 ttctttaggc tctaagtcta tttaataata ttttcatttt aggctcttcg tcagattggg
     7741 cagaggacga taagaacagc aagccgcagg cattttaaaa ataaagttcc ggagaagcaa
     7801 aaactgttcc aggtactgaa gtattttata ggagatgtta cttgtaatta ttagattacc
     7861 aaaaggtaag agttgggata aacaagtatg tgtataaatt agatcatatg acaatataaa
     7921 cattacaaaa aaagtcaagg acatgtacca tagtgctaat agtggttgtc tcttggggaa
     7981 agacctggtg gagcagagca atttaccttt ataagtagtt tgattatgag tgatttttgt
     8041 tttattatgg tttctagctt ttcctaaata aagcttaaag tgattaaaaa tagttgtatc
     8101 atatatacct aaaaatacaa tactttattg aacagatgta ctaaatagct actgtatgct
     8161 ggattagatg ctagggatat aatttagggt tataaaccaa aatcccttcc ccactttttt
     8221 aagggaaaaa aagtttccta tgactaaatc taatgtgtgg caattattgg ttcttttata
     8281 tttaagttat attaaagttg ttgagatcag tgtgtatgtt ttatagcttt aatgtttata
     8341 tattctttgg aaagaccgtc attaatggat ctcaaattaa cggtgaaaga agaatgctga
     8401 cttttaggaa ttcttatttt aaaagtagta gtagtagaga cggaataaag aaaattgtag
     8461 gtcttttaaa ttttaatact taaaatactt taattctagg gatttaagga gctatatcat
     8521 tttatatgcc tgttcttttg aaacttagtt ggaaatgagt gagacattta ttattgtctt
     8581 tttttttttt ttttaaatct aggaggatga tgaaattcca ctgtatctaa agggtggggt
     8641 agctgatgcc ctcctgtata gagccaccat gattcttaca gttggtggta aggcctttgg
     8701 aatgtttgaa ggtttataga aaagtaatag taatgataac tgataacttt attgcttttg
     8761 ctatgccagt attttaatgc aggcagtttg tctccagaat ttgatatctt actatatctc
     8821 ataatgattc aagagggtga ctgatacata ctgtataaga gcattgtgat gtcttctttt
     8881 ttttttgaaa tggagttttg ctcctgttgc ccaggctgga ttgcaatggc gcgatctcgg
     8941 ctcactgcaa cctctgcctc ccgggttcaa gcgattctcc tgcctcagcc tcccgagtag
     9001 ctgggattac aggcgtgcac caccatgcct ggctaatttt gtagttttag tagagacgga
     9061 gtttctccgt gttggtcagg ctggtcttga actcctgacc tcaggtgatc tgcccgccac
     9121 agcctcccaa agtattggga ttacaggcat gagccactgc gcctggcctt gatgtcttaa
     9181 taaaaaaata acaccagtaa gatttgatga ttatgttgtg atagagttgg ctttctgagt
     9241 cccattgatg gcagtgaagg ctatatttca gtcaaaaaca tccattatat cccacctctt
     9301 acagcaaggg ctttaaaaca ttttgactgt gatgcaaaat aaaagcacac tttacatcta
     9361 aactttgtac agacatattt atgaccaaaa caaaatttgt acagaacaat acttccactg
     9421 gaaggtaaag agaagggatg ctatatttga gtgattattt tatgcctatc atattcatag
     9481 gaatgccaca tacttggatt tctgtgagtc ttgcatgaag tctataatat gtcctcattt
     9541 taaagatgaa gaaatcatat taattacctg tttgggaaac tctactctca caccacaaca
     9601 atcaacatgg aagatctctg ttgccaaatg tgtgggggtt tttccctgca caccaagtaa
     9661 tggacaccag ctaggtgtcc tccagttcag tttccacact atctactggg agatagcatc
     9721 agattccaca ggttgatggc acaagactgt cccctccttc ccaccagttg caagtttggg
     9781 tcttcggaac ttctgactga ctggctttaa gttggggttc ccacaatctc ctcgttgggg
     9841 aaacacattt accagtttat tataaaaggt ataggctggg catggtggct catgcctgta
     9901 atcccagcac tttgggaggc tgaggctggc agatcacctg aggtcaggag ttcaagacta
     9961 gcctggcaca catggtgaaa ccctgtctgt actaaaaaca caaaaatgag tcgggtgtga
    10021 tggcacacac ttgcaatccc agctactcgt gaagctgagg caggaagatt gcttgaactg
    10081 gtaggcggag gttgcagtga gctcagattg tgtcactgca ctccagcctg ggcgacagag
    10141 ggagactcca tctcaaaaaa aagaaaaaaa aagataaagc cctaaattcc ttttggccaa
    10201 tgtgacattt cttagacatt ctggtaagta gtttttaaaa tgaaattcca gttatcagct
    10261 tttttttttt tttttttttt tttgagatgg agtttcgctc ttgttgccca ggctggagtg
    10321 caatggcgcg atctcggctt actgcaacct ctgcctccca ggttcaagca attctcctgc
    10381 ctcagcctcc caagtagctg ggattacagg catgcaccac cacgcctggc taattttgta
    10441 tttttagtag agacagggtt tctccatgtt gaggctggtc tcgaactcct gacctcaggt
    10501 gatccgcccg cctcggcctc ccaaagtgct gggattacag gtttcagcaa ctatgcccgg
    10561 cccagttatc agctattttt tatattacat ttgattactt taaaattata tcactctttt
    10621 tttttacatt cactttaggg aaggcttgca tattttgaac tctttttttc tttggcattt
    10681 aatcatcctt agcatttctc aggcagtctt gggttaatat ggaaataacc tttgtatacg
    10741 ttcttaatct tatttttaaa aatttttgca ttttatgttg acaaatatat tattgtgcca
    10801 cactttctat attaaaacta gattaaatat attttacata tatttattta aaatttttcc
    10861 agcacctatc aaaatacttt tttgtgttcc aattaaggta gtcttaatgc aaacttacaa
    10921 cttttgaact ggaatattaa atttatacac ttagatctta gaatttatgg ttaagttctt
    10981 ttttgttttt tatttttagg aacagcatat gccatatatg agctggctgt ggcttcattt
    11041 cccaagaagc aggagtgact tcagtcatcc cagcaatcgc ttggttcagt ttcattcagc
    11101 tctctatgga ccagtaatct gataaataac cgagctcttc tttggggatc aatatttatt
    11161 gacttgtagt aactgccacc aataaagcag tctttaccat gctttgtctg attttatcac
    11221 tttatgccaa taatttcaac ttttggtcag ccttgtaaat cttaggtaag gatcaaagaa
    11281 atgattcttt tgccactaaa cttgtgaaag caacattatt gtgatgttat tttagtgaaa
    11341 ttttacttct aacagctcaa aattagtctc aacttctgtc tcaacttgcc gtttgaatgc
    11401 atattgcttt ttccccctac ccactccagt atatcttgga atatgctaga acccgggggt
    11461 agtggaagca gattatcttt ggctcctggg gtctatattt cttttttttt ttttttttct
    11521 cagctatgtg agagtatgct taaatattgg atatatccca cagcttagaa ttaggcttta
    11581 gtagaatggc tttgtaagat atttcagtat ttgttttttt ctacactccg agtgggttca
    11641 ggtaggtttc tctcatcagg aaccaaccaa agcaggacag ttcctgggct ccccagctat
    11701 catctaatgg acccacaact gtttaaccta aacagtagtt aggtgcttaa taaatggtag
    11761 ccataatagg ctgctcataa taggaatgtt tcaaaaaggt ctatttaggg aacacaaaat
    11821 ccaaatagat acagagagta agaatcaaaa agaattacga tgctgctcta ggtagtttgc
    11881 ttcagggttt gcagaaccct tcctgctttg agagaactat gcatgaagct ggaaatttca
    11941 tagacttcaa gtttgggact cccttttcct gggtcctgtt gtgcatccat gtggagagta
    12001 gcatcttgtg aaccacttca ggactttcct tctatatata tcgagattgg taattcgtca
    12061 agagatactg agtgcctact gtgtcctaac tggttttagg tacctaggaa tcactggtga
    12121 acaaaagcag attcttcccc tcatatgtta tagaaagggg tggtaggaaa gagagtttaa
    12181 aaataaattg cacagtaggt taaaagataa gttctatgga aaaagaccaa aggggagaac
    12241 tcacatgggt gtggttggtt ggttgcagta ttaaagttag ggtaggcttc aacaggaagg
    12301 tcagattgtt taaaccaagc ggcgactgag atgtcctaaa tgcacagcga cggattcgcg
    12361 ctatttagaa agagagagca atatttcaag aatgcatgcg tcaattttac gcagactatc
    12421 tttctagggt taatctagct gcatcaggat catatcgtcg ggtctttttt ccggctcagt
    12481 catcgcccaa gctggcgcta tctgggcatc ggggaggaag aagcccgtgc cttttcccgc
    12541 gaggttgaag cggcatggaa agagtttgcc gaggatgact gctgctgcat tgacgttgag
    12601 cgaaaacgca cgtttaccat gatgattcgg gaaggtgtgg ccatgcacgc ctttaacggt
    12661 gaactgttcg ttcaggccac ctgggatacc agttcgtcgc ggcttttccg gacacagttc
    12721 cggatggtca gcccgaagcg catcagcaac ccgaacaata ccggcgacag ccggaactgc
    12781 cgtgccggtg tgcagattaa tgacagcggt gcggcgctgg gatattacgt cagcgaggac
    12841 gggtatcctg gctggatgcc gcagaaatgg acatggatac cccgtgagtt acccggcggg
    12901 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
    12961 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
    13021 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
    13081 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
    13141 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
    13201 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    13261 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    13321 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    13381 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    13441 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    13501 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    13561 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    13621 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    13681 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    13741 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
    13801 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    13861 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    13921 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    13981 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    14041 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    14101 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    14161 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    14221 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    14281 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    14341 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    14401 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    14461 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    14521 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    14581 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    14641 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    14701 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    14761 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    14821 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    14881 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    14941 t
//
