LOCUS       Exported               14111 bp ds-DNA     circular SYN 22-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14111)
  AUTHORS   The Weissman lab
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 6, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14111
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          12084..12113
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /note="/locus_tag=source:synthetic DNA construct"
     source          13475..13493
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /note="/locus_tag=source:synthetic DNA construct"
     LTR             91..324
                     /locus_tag="3' LTR (Delta-U3)"
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR)
                     from HIV-1"
     promoter        complement(349..367)
                     /locus_tag="SP6 promoter"
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(350..367)
                     /locus_tag="SP6"
                     /label=SP6
     promoter        1155..1259
                     /gene="bla"
                     /locus_tag="AmpR promoter"
                     /label=AmpR promoter
     CDS             1458..2117
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      2291..2879
                     /direction=RIGHT
                     /locus_tag="ori"
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3125..3454
                     /locus_tag="SV40 promoter"
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     intron          4588..4653
                     /locus_tag="small t intron"
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4783..4803
                     /codon_start=1
                     /locus_tag="SV40 NLS"
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5228..5362
                     /locus_tag="SV40 poly(A) signal"
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             5531..6164
                     /locus_tag="3' LTR"
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6211..6336
                     /locus_tag="HIV-1 Psi"
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    6833..7065
                     /locus_tag="RRE"
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     repeat_region   7553..7732
                     /locus_tag="cPPT"
                     /label=cPPT
     primer_bind     7708..7731
                     /locus_tag="cPPT_5'seq"
                     /label=cPPT_5'seq
     misc_feature    complement(7770..7778)
                     /locus_tag="CTCF binding site"
                     /label=CTCF binding site
     misc_feature    7945..7958
                     /locus_tag="CTCF binding site(1)"
                     /label=CTCF binding site
                     /label=CTCF binding site(1)
     misc_feature    7997..8435
                     /locus_tag="UCOE"
                     /label=UCOE
     misc_feature    8368..8376
                     /locus_tag="missing in actual plasmid"
                     /label=missing in actual plasmid
     misc_feature    8394
                     /label=potential c->A?
     misc_feature    8435..8436
                     /locus_tag="origin"
                     /label=origin
     misc_feature    8470..8473
                     /label=from Sanger seq
     promoter        8474..9690
                     /label=hUbC_promoter
     misc_feature    9263
                     /label=from Sanger seq
     misc_feature    9308
                     /label=from Sanger seq
     misc_feature    9699..12074
                     /label=AKAP1
     misc_feature    10041..10060
                     /label=gRNA2
     misc_feature    11817
                     /label=Tudor domain_deleted
     CDS             12114..12143
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    12153..12739
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             12752..13462
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     13494..13516
                     /locus_tag="1167_seq"
                     /label=1167_seq
     enhancer        13503..14094
                     /locus_tag="WPRE"
                     /label=WPRE
     CDS             complement(13975..13986)
                     /codon_start=1
                     /locus_tag="Factor Xa site"
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(14095..14111)
                     /locus_tag="KS primer"
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agggaattaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
       61 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
      121 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
      181 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      241 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      301 agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca
      361 cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga
      421 caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct
      481 ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc
      541 cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct
      601 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
      661 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
      721 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
      781 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
      841 cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct
      901 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
      961 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     1021 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     1081 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     1141 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     1201 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     1261 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     1321 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     1381 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     1441 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     1501 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     1561 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     1621 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     1681 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     1741 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     1801 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     1861 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     1921 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     1981 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     2041 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     2101 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     2161 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     2221 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     2281 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     2341 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2401 taactggctt cagcagagcg cagataccaa atactgtctt tctagtgtag ccgtagttag
     2461 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2521 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2581 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2641 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2701 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2761 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2821 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2881 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2941 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     3001 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     3061 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
     3121 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3181 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     3241 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3301 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3361 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     3421 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
     3481 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
     3541 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
     3601 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
     3661 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
     3721 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
     3781 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
     3841 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
     3901 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
     3961 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
     4021 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
     4081 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
     4141 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
     4201 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
     4261 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
     4321 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
     4381 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
     4441 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
     4501 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
     4561 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
     4621 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
     4681 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
     4741 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
     4801 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
     4861 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
     4921 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
     4981 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
     5041 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
     5101 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
     5161 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     5221 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5281 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5341 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     5401 tcccacccca taccctatta ccactgccaa ttacctagtg gtttcattta ctctaaacct
     5461 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     5521 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
     5581 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
     5641 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
     5701 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
     5761 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
     5821 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
     5881 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
     5941 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
     6001 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6061 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6121 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     6181 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     6241 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     6301 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     6361 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     6421 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     6481 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     6541 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     6601 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     6661 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     6721 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     6781 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     6841 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     6901 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     6961 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     7021 caagaatcct ggctgtgaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     7081 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     7141 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     7201 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     7261 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     7321 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     7381 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     7441 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     7501 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat
     7561 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     7621 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     7681 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa
     7741 gcttgatatc gaattcggga ggtggtccct gcagttacgc caatgataac ccccgccaga
     7801 aaaatcttag tagccttccc tttttgtttt ccgtgcccca actcggcgga ttgactcggc
     7861 cccttccgga aacacccgaa tcaacttcta gtcaaattat tgttcacgcc gcaatgaccc
     7921 acccctggcc cgcgtctgtg gaactgaccc ctggtgtaca ggagagttcg ctgctgaaag
     7981 tggtcccaaa ggggtactag tttttaagct cccaactccc cctcccccag cgtctggagg
     8041 attccacacc ctcgcaccgc aggggcgagg aagtgggcgg agtccggttt tggcgccagc
     8101 cgctgaggct gccaagcaga aaagccaccg ctgaggagac tccggtcact gtcctcgccc
     8161 cgcctccccc ttccctcccc ttggggacca ccgggcgcca cgccgcgaac ggtaagtgcc
     8221 gcggtcgtcg gcgcctccgc cctcccccta gggccccaat tcccagcggg cgcggcgcgc
     8281 ggcccctccc cccgccgggc gcgcgcccgc tgccccgccc ttcgtggccg cccggcgtgg
     8341 gcggtgccac ccctcccccc ggcggccccc ctgcagccgc gcgcagctcc cggctccctc
     8401 ccccttcgga tgtggcttga gctgtaggcg cggaggacgt agccatgctc taggaagatc
     8461 ggaattcctt ccagatctgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc
     8521 tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg
     8581 cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc
     8641 agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag
     8701 agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt
     8761 ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt
     8821 cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga
     8881 gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga
     8941 agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac
     9001 tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc
     9061 ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac
     9121 ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg
     9181 gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc
     9241 tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc
     9301 gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc
     9361 cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta
     9421 gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg
     9481 cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt
     9541 tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa
     9601 atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa
     9661 ttctggccgt ttttggcttt tttgttagac gcggccgcat ggcaatccag ttccgttcgc
     9721 tcttcccctt ggcattgcct gggatgctgg cgctcctcgg ctggtggtgg tttttctctc
     9781 gtaaaaaagg ccatgtcagc agccatgatg agcagcaggt ggaggctggt gctgtgcagc
     9841 tgagggctga ccctgccatc aaggaacctc tccccgtgga agacgtctgt cccaaagtag
     9901 tgtccacacc ccccagtgtc acagagcctc cagaaaagga actgtccacc gtgagcaagc
     9961 tgcctgcaga gcccccagca ttgctccaga cacacccacc ttgccgaaga tcagagtcct
    10021 cgggcattct tcctaacacc acagacatga gacttcggcc aggaacacgc agagatgaca
    10081 gtacaaagct ggagctagcc ctgacaggtg gtgaagccaa atcgattcct ctagagtgcc
    10141 ccctttcatc cccaaagggt gtactattct ccagcaaatc agctgaggtg tgtaagcaag
    10201 attccccctt cagcagggtg ccaaggaagg tccagccagg ctaccccgta gtccccgcag
    10261 agaagcgtag ctctggggag agggcaagag agacaggtgg ggccgaaggg actggtgatg
    10321 ccgtgttggg ggaaaaggtg cttgaagaag ctctgttgtc tcgggagcat gtcttggaat
    10381 tggagaacag caagggcccc agcctggcct ctttagaggg ggaagaagat aaggggaaga
    10441 gcagctcatc ccaggtggtg gggccagtgc aggaggaaga gtatgtagca gagaagttgc
    10501 caagtaggtt catcgagtcg gctcacacag agctggcaaa ggacgatgcg gcgccagcac
    10561 ccccagtcgc agacgccaaa gcccaggata gaggtgtcga gggagaactg ggcaatgagg
    10621 agagcttgga tagaaatgag gagggcttgg atagaaatga ggagggcttg gatagaaatg
    10681 aggagagctt ggatagaaat gaggagggct tggatagaaa tgaggagatt aagcgggctg
    10741 ccttccagat aatctcccaa gtgatctcag aagcaaccga acaggtgctg gccaccacgg
    10801 ttggcaaggt tgcaggtcgt gtgtgtcagg ccagtcagct ccaagggcag aaggaagaga
    10861 gctgtgtccc agttcaccag aaaactgtct tgggcccaga cactgcggag cctgccacag
    10921 cagaggcagc tgttgccccg ccggatgctg gcctcccctt gccaggccta ccagcagagg
    10981 gctcaccacc accaaagacc tacgtgagct gcctgaagag ccttctgtcc agccccacca
    11041 aggacagtaa gccaaatatc tctgcacacc acatctccct ggcctcctgc ctggcactga
    11101 ccacccccag tgaagagttg ccggaccggg caggcatcct ggtggaagat gccacctgtg
    11161 tcacctgcat gtcagacagc agccaaagtg tccctttggt ggcttctcca ggacactgct
    11221 cagattcttt cagcacttca gggcttgaag actcttgcac agagaccagc tcgagcccca
    11281 gggacaaggc catcaccccg ccactgccag aaagtactgt gcccttcagc aatggggtgc
    11341 tgaaggggga gttgtcagac ttgggggctg aggatggatg gaccatggat gcggaagcag
    11401 atcattcagg aggttctgac aggaacagca tggattccgt ggatagctgt tgcagtctca
    11461 agaagactga gagcttccaa aatgcccagg caggctccaa ccctaagaag gtcgacctca
    11521 tcatctggga gatcgaggtg ccaaagcact tagtcggtcg gctaattggc aagcaggggc
    11581 gctatgtgag ttttctgaag caaacatctg gtgccaagat ctacatttca accctgcctt
    11641 acacccagag cgtccagatc tgccacatag aaggctctca acatcatgta gacaaagcgc
    11701 tgaacttgat tgggaagaag ttcaaagagc tgaacctcac caatatctac gctcccccat
    11761 tgccttcact ggcactgcct tctctgccga tgacatcctg gctcatgctg cctgatccgt
    11821 ttcagggagc agaagtcctt ctggacagtg tgatgcccct gtcagacgat gaccagtttt
    11881 caccggaagc agatgccgcc atgagcgaga tgacggggaa tacagcactg cttgctcagg
    11941 tgacaagtta cagtccaact ggtcttcctc tgattcagct gtggagtgtg gttggagatg
    12001 aagtggtgtt gataaaccgg tccctggtgg agcgaggcct tgcccagtgg gtagacagct
    12061 actacacaag ccttggaggt tctggtggag gcgggtctgg aggcgggggt agtgagcaga
    12121 aactcatctc tgaagaggat ctgtgaacgc gtcccctctc cctccccccc ccctaacgtt
    12181 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
    12241 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
    12301 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
    12361 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
    12421 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
    12481 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
    12541 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
    12601 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
    12661 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
    12721 tgataatatg gccacaacca tggcgtccgg aatggtgagc aagggcgagg aggataacat
    12781 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
    12841 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
    12901 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
    12961 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
    13021 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
    13081 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
    13141 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
    13201 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
    13261 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
    13321 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
    13381 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
    13441 catggacgag ctgtacaagt aataagcgac gcggcatgca agcttgatat caagcttatc
    13501 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
    13561 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
    13621 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
    13681 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
    13741 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
    13801 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
    13861 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
    13921 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
    13981 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
    14041 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
    14101 cgtcgacctc g
//
