LOCUS       Exported               13111 bp ds-DNA     circular SYN 22-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 6, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13111
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     PCR_primer      405..411
                     /label=ACGCCAA
     primer_bind     621..640
                     /label=HKO084
     LTR             828..1016
                     /label=5' LTR
     misc_feature    1067..1204
                     /label=Psi
     misc_feature    1124..1127
                     /label=SD
                     /label=SD; splice donor
     primer_bind     2408..2427
                     /label=HKO074
     misc_feature    2440..2557
                     /label=cPPT
     misc_feature    complement(2607..3200)
                     /label=WPRE
                     /label=WPRE; Woodchuck post-transcriptional
     primer_bind     complement(3100..3119)
                     /label=HKO083
     CDS             complement(3223..3822)
                     /label=Puro (-)
     promoter        complement(3835..4337)
                     /label=PGK (-)
     promoter        4344..5602
                     /label=EF1a
     misc_feature    5574..5602
                     /label=homology/F primer for 5'UTR
     misc_feature    5611..5753
                     /label=ALDH18A1-5'UTR
     misc_feature    5733..5753
                     /label=R primer for 5'UTR
     CDS             5754..6053
                     /codon_start=1
                     /label=TBRG4-isoform1-CDS
                     /translation="MAAHLVKRCTCLLREAARQAPAMAPVGRLRLAWVAHKTLTSSATS
                     PISHLPGSLMEPVEKERASTPYIEKQVDHLIKKATRPEELLELLGGSHDLDSNQA"
     misc_feature    6032..6053
                     /label=R primer
     CDS             6054..6083
                     /codon_start=1
                     /label=TBRG4-isoform1-CDS
                     /translation="AMVLIRLSHL"
     misc_feature    6084..6833
                     /label=XTEN250-actual
     misc_feature    6084..6103
                     /label=homology
     CDS             6834..7676
                     /codon_start=1
                     /label=TBRG4-isoform1-CDS
                     /translation="EAFAQHVLNRAQDITLPHLCSVLLAFARLNFHPDQEDQFFSLVHE
                     KLGSELPGLEPALQVDLVWALCVLQQAREAELQAVLHPEFHIQFLGGKSQKDQNTFQKL
                     LHINATALLEYPEYSGPLLPASAVAPGPSALDRKVTPLQKELQETLKGLLGSADKGSLE
                     VATQYGWVLDAEVLLDSDGEFLPVRDFVAPHLAQPTGSQSPPPGSKRLAFLRWEFPNFN
                     SRSKDLLGRFVLARRHIVAAGFLIVDVPFYEWLELKSEWQKGAYLKDKMRKAVAEELAK
                     "
     misc_feature    6834..7676
                     /label=351aa to ened
     misc_feature    6834..6851
                     /label=F primer for TBRG4 frag 2
     misc_feature    7680..7701
                     /label=F primer for 3'UTR
     CDS             7680..7700
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="SLLKQA"
     misc_feature    7721..8521
                     /label=ALDH18A1-3'UTR
     misc_feature    8529..8556
                     /label=homology/R primer for 3'UTr
     primer_bind     8654..8674
                     /label=HKO085
     LTR             8909..9097
                     /label=3' LTR
     PCR_primer      complement(9415..9431)
                     /direction=LEFT
                     /label=CCCGCCGCGCTTAATGC
     PCR_primer      9671..9698
                     /label=CGTTGGAGTCCACGTTCTTTAATAGTGG
     PCR_primer      complement(11333..11352)
                     /direction=LEFT
                     /label=AGCAACGCGGCCTTTTTACG
     PCR_primer      complement(11390..11418)
                     /direction=LEFT
                     /label=CACCTCTGACTTGAGCGTCGATTTTTGTG
     rep_origin      11546..11771
                     /label=ColE1
     PCR_primer      complement(11804..11829)
                     /direction=LEFT
                     /label=TTCAGCAGAGCGCAGATACCAAATAC
     PCR_primer      complement(12137..12159)
                     /direction=LEFT
                     /label=CAGATCGCTGAGATAGGTGCCTC
     PCR_primer      complement(12637..12662)
                     /direction=LEFT
                     /label=TCACAGAAAAGCATCTTACGGATGGC
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagcgatg cggggaggcg gcccaaaggg agatccgact
     2641 cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga
     2701 aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat
     2761 ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg acaacaccac
     2821 ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat
     2881 gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt
     2941 ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg
     3001 ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga
     3061 ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta
     3121 tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt
     3181 gtaatccaga ggttgattac cgataagctt gatatcgaat tcttaactgg caccgggctt
     3241 gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa
     3301 gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac
     3361 cccggcgcgc tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc
     3421 ctggtggtcg ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg
     3481 cggcgccagg aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc
     3541 catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca
     3601 gaccgccacg gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg
     3661 cgtgaggaag agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt
     3721 gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac
     3781 gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg gtcatggtgg taccccctgg
     3841 ggagagaggt cggtgattcg gtcaacgagg gagccgactg ccgacgtgcg ctccggaggc
     3901 ttgcagaatg cggaacaccg cgcgggcagg aacagggccc acactaccgc cccacacccc
     3961 gcctcccgca ccgccccttc ccggccgctg ctctcggcgc gccctgctga gcagccgcta
     4021 ttggccacag cccatcgcgg tcggcgcgct gccattgctc cctggcgctg tccgtctgcg
     4081 agggtactag tgagacgtgc ggcttccgtt tgtcacgtcc ggcacgccgc gaaccgcaag
     4141 gaaccttccc gacttagggg cggagcagga agcgtcgccg gggggcccac aagggtagcg
     4201 gcgaagatcc gggtgacgct gcgaacggac gtgaagaatg tgcgagaccc agggtcggcg
     4261 ccgctgcgtt tcccggaacc acgcccagag cagccgcgtc cctgcgcaaa cccagggctg
     4321 ccttggaaaa ggcgcaaatc gattgcaaag atggataaag ttttaaacag agaggaatct
     4381 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca
     4441 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     4501 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     4561 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     4621 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     4681 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     4741 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     4801 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     4861 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     4921 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     4981 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     5041 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     5101 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     5161 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     5221 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     5281 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     5341 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     5401 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     5461 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     5521 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     5581 tcttccattt caggtgtcgt gacctgcagg atcgtgcggg acgtggacgg aagaaaaaag
     5641 agagtgagcg agcgccggaa tcagcccggc gtggagtgcg gacccgcggt agtgccaggg
     5701 ggcgaaggcg gcggtggtga ggaagatact ttggttagtg accacatcgc agcatggcag
     5761 ctcacctggt aaagcgatgc acgtgcctcc tgagagaagc tgctcgtcag gcccctgcca
     5821 tggctccagt tggccgactg agacttgcct gggtagccca taagactctg acttcctcag
     5881 ccacctcacc catttcccac ctcccaggtt ccttgatgga gccggtggag aaggaacgag
     5941 catctactcc ctacatagag aagcaggtgg accacctcat caagaaggcc acaaggccag
     6001 aggagctcct ggagctactt ggtggcagtc acgacttgga cagcaatcaa gcagcaatgg
     6061 tacttatccg gctctctcac ttggggtcag aacctgcaac atcaggatcc gagaccccgg
     6121 ggacgtcaga gtcagcaacc cccgaatctg gaccaggcac gagcactgag ccgtcagaag
     6181 ggtcagcacc tggtagtcca gccggaagtc caacgagcac agaagagggc acaagtgagt
     6241 ccgcaacccc cgagagcggc ccaggaagcg agcctgcgac atccggcagc gagaccccgg
     6301 gcacctcaga atcagcaact ccggagagtg gaccgggtag cccagccgga tctcccacta
     6361 gtacggaaga ggggtccccc gcaggctcac ccacgtccac cgaggagggt acttctacgg
     6421 aaccttctga ggggtccgca ccaggtacct ctgaatctgc aacgcccgag tccggtccag
     6481 gaacgtcaga gagtgcaact ccggaatctg gcccgggcac cagtgaatct gccactcctg
     6541 aatccggtcc gggctctgag ccagccacat cagggtctga aacaccgggg agcgaacccg
     6601 cgactagtgg aagtgaaaca cctgggtccc cagccgggtc tccgaccagt actgaggagg
     6661 gaacgtcaac agaaccatcc gagggtagcg cccctgggac ttcaacggag cctagtgaag
     6721 gcagcgcgcc ggggtctgaa cccgcgacca gtggtagcga gacgccgggc acatctgagt
     6781 ccgccacacc agagtctggg cccggtacga gtacagaacc atctgagggc agtgaggcct
     6841 ttgcccagca cgtcctgaac agagcgcagg acatcaccct gccccacctg tgcagcgtac
     6901 ttctggcttt tgcgcgtctg aacttccatc cagaccaaga ggatcagttc ttcagcctgg
     6961 tacatgagaa gctggggtca gagctgccag gcctggagcc agccctgcag gtggacctgg
     7021 tgtgggccct gtgtgtgctg cagcaggcac gggaagcaga gctgcaagcc gtcctccacc
     7081 ctgaatttca catccaattt ctagggggca agtctcagaa ggatcagaac accttccaga
     7141 agctgctcca catcaacgcc actgccctgc tggagtaccc cgagtactcg ggtccccttc
     7201 tgcctgcctc ggctgtggcc cctgggccct cagcccttga caggaaggtg acccccctgc
     7261 aaaaggagct gcaggagacg ctgaaggggc tgctggggag cgccgacaag ggcagcctcg
     7321 aggtggccac gcagtatggc tgggtgctgg atgctgaggt gctgctggac agtgacggcg
     7381 agtttctgcc cgtaagggac tttgtggcac ctcaccttgc ccagccaact gggagccagt
     7441 caccacctcc agggtctaag aggctagcgt tcttgcggtg ggagttcccc aacttcaaca
     7501 gccgaagcaa ggacttgctg ggtcgctttg ttctggcccg gcgacacata gtggctgcag
     7561 gcttcctgat agtggacgtc ccattctatg agtggctgga actcaagtct gaatggcaga
     7621 aaggcgccta cctcaaggac aagatgcgca aagcggtggc tgaggagctg gccaagtgat
     7681 tagtctgctg aaacaagccg cctgaacgcg ttaagtcgac aaagagccag gaaaacccgg
     7741 gaattttcca aaaggtcttc acgttaaact tgtcttatct caggagagag cccgctcttg
     7801 tctcccagtt cctggtaggg tctgcctgtt ggaaagtgta cctggatgct tctgggctcc
     7861 gtttggcaat agcagtcttg gctgatgtgc acagtctggc tcccagctca cccttttttt
     7921 ttaaagtaag aaaatagttg ctaccgatag ggactttgcc aagtccaatt atcttctagg
     7981 attgaaaggt gcattttccc cataaaaaag gcgaggaaaa cccatggctg ctttgtgtca
     8041 cctcagtgac ttacagtccc ccttggcatt tagttggtac tagagccagt catccttaac
     8101 aaatcttttc acattttatt tctttcacat gtagtcatct tcaaaaagga aagatttgga
     8161 attttagaaa aggggcaact cttcttttta gcattctcat cagaaagtca caaaaatcga
     8221 tggaatcatt tccactggga agattgacct tttgtattta tttgtggggt aaattaataa
     8281 gcattccaga tgcttgcagc ttcctgcatc caggagatgc tgtgttcccc gtgatgcagc
     8341 tggaacccaa gctgcagcag gagatgcaag tttcaggatg ttccccactg agctggagga
     8401 atatctacag cagtgatgct tgaaattttt gtatgaatta ttttgtcgtc ctaccctttt
     8461 cctccaaaac aaaaattaga ggattatttt aatactttgg attcttcccc cttttttgag
     8521 attaattaac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     8581 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     8641 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     8701 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     8761 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     8821 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     8881 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     8941 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9001 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9061 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
     9121 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     9181 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     9241 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     9301 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     9361 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     9421 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     9481 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     9541 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     9601 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     9661 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     9721 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     9781 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     9841 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     9901 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     9961 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
    10021 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
    10081 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
    10141 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
    10201 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
    10261 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
    10321 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
    10381 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
    10441 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
    10501 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
    10561 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
    10621 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
    10681 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
    10741 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
    10801 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    10861 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
    10921 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
    10981 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    11041 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    11101 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    11161 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    11221 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    11281 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    11341 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    11401 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    11461 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    11521 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    11581 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    11641 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    11701 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    11761 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    11821 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    11881 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    11941 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    12001 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    12061 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    12121 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    12181 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    12241 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    12301 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    12361 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    12421 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    12481 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    12541 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    12601 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    12661 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    12721 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    12781 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    12841 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    12901 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    12961 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    13021 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    13081 gcgcacattt ccccgaaaag tgccacctga c
//
