LOCUS       CAG-mTfeb               7878 bp    DNA     circular SYN 10-APR-2020
DEFINITION  synthetic circular DNA
ACCESSION   pVec1
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 22, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    33..136
                     /label=AAV ITR
     enhancer        278..657
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        660..935
                     /label=chicken beta-actin promoter
     CDS             1487..1513
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    1517..2947
                     /label=mTfeb opt
     misc_feature    2963..3551
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3434..3445)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3707..3762
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    4160..4263
                     /label=AAV ITR
     rep_origin      4661..5174
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5921..6025
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6026..6094,6095..6886)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7057..7645
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
      121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca
      181 tgctctagcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat gagcttggcc
      241 cattgcatac gttgtatcca tatcataata tgtactcgac attgattatt gactagttat
      301 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      361 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      421 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      481 gactatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      541 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      601 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatgggt
      661 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
      721 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggc
      781 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg
      841 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
      901 ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgttg ccttcgcccc
      961 gtgccccgct ccgcgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc
     1021 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat
     1081 gacggctcgt ttcttttctg tggctgcgtg aaagccttaa agggctccgg gagggccctt
     1141 tgtgcggggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg
     1201 tgcggcccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc
     1261 tccgcgtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggggga cggctgcctt
     1321 cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc
     1381 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
     1441 gttgtgctgt ctcatcattt tggcaaagaa ttctcgagcc acaatgtacc cttacgacgt
     1501 gccagactac gccggaatgg ctagccgaat aggactgagg atgcagctta tgagagagca
     1561 ggcccaacaa gaggagcaga gagagagaat gcaacagcaa gctgtgatgc actacatgca
     1621 acagcaacag caacaacaac agcagctggg gggccctcca accccagcca ttaatactcc
     1681 tgtccacttt cagtcccctc cccctgtccc tggagaagtt ctcaaagttc agagttatct
     1741 cgagaatcct acatcctatc acctccagca aagtcaacac cagaaggtgc gagagtatct
     1801 ctctgagact tatggcaaca agtttgctgc tcatgtaagt cccgcccaag gctctccaaa
     1861 acctgccccc gctgctagtc ccggagtacg cgctggccat gttctttcaa cttccgccgg
     1921 taactctgca cctaatagtc caatggctat gcttcacatc agcagcaacc cagaaaagga
     1981 atttgacgat gttatagata atatcatgag gcttgattcc gtactcgggt acattaaccc
     2041 agaaatgcaa atgccaaaca cccttcccct cagcagctca catctgaatg tatactccgg
     2101 cgatccacaa gtgaccgcct ccatggtagg ggtgaccagc tctagttgtc ctgctgattt
     2161 gactcagaaa agggagttga ctgatgcaga gagtcgagcc ctcgcaaaag agcgacagaa
     2221 aaaagacaat cataacctta tagagcgacg gcgcaggttc aatattaatg accgcatcaa
     2281 ggagttggga atgttgatcc ctaaagctaa tgatctcgat gtgcggtgga acaaaggtac
     2341 catactcaaa gccagtgttg attacataag gcgcatgcaa aaagacctcc aaaaatccag
     2401 agaactggaa aatcattccc gcaggctgga aatgacaaat aaacaactgt ggctgcggat
     2461 ccaagaactt gagatgcaag cacgagtgca cggtttgccc actacaagtc ccagtggcgt
     2521 taatatggcc gagcttgccc aacaggtggt aaagcaagag ctgccctccg aggatgggcc
     2581 aggggaagcc cttatgcttg gtccagaggt gccagagcca gagcagatgc ctgctctccc
     2641 tcctcaagca cctctgccct ctgctgctca gccccaatca ccttttcacc acctcgattt
     2701 ctctcacggg ctgagctttg ggggcggggg cgatgaagga ccaactggtt atccagatac
     2761 cctcggaaca gagcatggct ctccattccc taaccttagc aagaaagacc tggatctgat
     2821 gttgctcgat gacagccttc tgcctctcgc cagcgaccca ctctttagca caatgtctcc
     2881 agaggcctca aaggcaagct ctaggagaag ttcattctct atggaagaag gagacgtgtt
     2941 gtagtgaatc ggtaccggat ccaatcaacc tctggattac aaaatttgtg aaagattgac
     3001 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     3061 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     3121 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     3181 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     3241 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     3301 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     3361 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     3421 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     3481 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     3541 cgcctccccg cctggatcca attcactcct caggtgcagg ctgcctatca gaaggtggtg
     3601 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
     3661 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     3721 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     3781 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     3841 atatgctggc tgccatgaac aaaggtggct ataaagaggt catcagtata tgaaacagcc
     3901 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     3961 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     4021 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     4081 aagatccctc gacctgcagc ccaagctcta gagcatggct acgtagataa gtagcatggc
     4141 gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     4201 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     4261 gcggcctcag tgagcgagcg agcgcgccag ctggcgtaat agcgaagagg cccgcaccga
     4321 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgattctgtt gcaatggctg
     4381 gcggtaatat tgttctggat attaccagca aggccgatag tttgagttct tctactcagg
     4441 caagtgatgt tattactaat caaagaagta ttgcgacaac ggttaatttg cgtgatggac
     4501 agactctttt actcggtggc ctcactgatt ataaaaacac ttctcaggat tctggcgtac
     4561 cgttcctgtc taaaatccct ttaatcggcc tcctgtttag ctcccgctct gattctaacg
     4621 aggaaagcac gttatacgtg ctcgtcaaag caaccatagt acgcgccctg tagcggcgca
     4681 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4741 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4801 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4861 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4921 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4981 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5041 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     5101 ttaacgttta caatttaaat atttgcttat acaatcttcc tgtttttggg gcttttctga
     5161 ttatcaaccg gggtacatat gattgacatg ctagttttac gattaccgtt catcgattct
     5221 cttgtttgct ccagactctc aggcaatgac ctgatagcct ttgtagagac ctctcaaaaa
     5281 tagctaccct ctccggcatg aatttatcag ctagaacggt tgaatatcat attgatggtg
     5341 atttgactgt ctccggcctt tctcacccgt ttgaatcttt acctacacat tactcaggca
     5401 ttgcatttaa aatatatgag ggttctaaaa atttttatcc ttgcgttgaa ataaaggctt
     5461 ctcccgcaaa agtattacag ggtcataatg tttttggtac aaccgattta gctttatgct
     5521 ctgaggcttt attgcttaat tttgctaatt ctttgccttg cctgtatgat ttattggatg
     5581 ttggaatgcc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     5641 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     5701 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     5761 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     5821 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     5881 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     5941 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6001 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     6061 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6121 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     6181 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     6241 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     6301 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     6361 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     6421 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     6481 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     6541 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     6601 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     6661 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     6721 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     6781 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     6841 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     6901 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6961 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7021 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7081 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7141 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7201 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7261 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7321 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7381 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7441 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7501 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7561 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7621 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     7681 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     7741 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     7801 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     7861 ttggccgatt cattaatg
//
