LOCUS       CAG-GFP                 7167 bp    DNA     circular SYN 10-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   pVec1
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 22, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    33..136
                     /label=AAV2 ITR
     enhancer        278..657
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    451..1210
                     /label=CAG Promoter
     promoter        660..935
                     /label=chicken beta-actin promoter
     misc_feature    1211..1467
                     /label=CAG Artificial Intron
     regulatory      1505..1514
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1511..1513,1514..1516,1517..2230)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    2252..2840
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(2723..2734)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2996..3051
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    3449..3552
                     /label=AAV2 ITR
     rep_origin      3950..4463
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5210..5314
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5315..5383,5384..6175)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6346..6934
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
      121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca
      181 tgctctagcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat gagcttggcc
      241 cattgcatac gttgtatcca tatcataata tgtactcgac attgattatt gactagttat
      301 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      361 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      421 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      481 gactatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      541 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      601 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatgggt
      661 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
      721 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggc
      781 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg
      841 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
      901 ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgttg ccttcgcccc
      961 gtgccccgct ccgcgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc
     1021 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat
     1081 gacggctcgt ttcttttctg tggctgcgtg aaagccttaa agggctccgg gagggccctt
     1141 tgtgcggggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg
     1201 tgcggcccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc
     1261 tccgcgtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggggga cggctgcctt
     1321 cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc
     1381 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
     1441 gttgtgctgt ctcatcattt tggcaaagaa ttctcgagct caagcttcga attctgcagt
     1501 cgacgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     1561 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     1621 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     1681 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     1741 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     1801 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     1861 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     1921 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     1981 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     2041 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     2101 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     2161 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     2221 gtacaagtaa tgagatatcg gtaccggatc caatcaacct ctggattaca aaatttgtga
     2281 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     2341 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     2401 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     2461 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     2521 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     2581 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     2641 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     2701 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     2761 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     2821 cctttgggcc gcctccccgc ctggatccaa ttcactcctc aggtgcaggc tgcctatcag
     2881 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     2941 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     3001 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     3061 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     3121 acatatgcca tatgctggct gccatgaaca aaggtggcta taaagaggtc atcagtatat
     3181 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
     3241 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
     3301 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
     3361 tctcttatga agatccctcg acctgcagcc caagctctag agcatggcta cgtagataag
     3421 tagcatggcg ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc
     3481 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     3541 tttgcccggg cggcctcagt gagcgagcga gcgcgccagc tggcgtaata gcgaagaggc
     3601 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gattctgttg
     3661 caatggctgg cggtaatatt gttctggata ttaccagcaa ggccgatagt ttgagttctt
     3721 ctactcaggc aagtgatgtt attactaatc aaagaagtat tgcgacaacg gttaatttgc
     3781 gtgatggaca gactctttta ctcggtggcc tcactgatta taaaaacact tctcaggatt
     3841 ctggcgtacc gttcctgtct aaaatccctt taatcggcct cctgtttagc tcccgctctg
     3901 attctaacga ggaaagcacg ttatacgtgc tcgtcaaagc aaccatagta cgcgccctgt
     3961 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4021 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4081 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4141 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4201 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4261 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4321 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4381 aacaaaatat taacgtttac aatttaaata tttgcttata caatcttcct gtttttgggg
     4441 cttttctgat tatcaaccgg ggtacatatg attgacatgc tagttttacg attaccgttc
     4501 atcgattctc ttgtttgctc cagactctca ggcaatgacc tgatagcctt tgtagagacc
     4561 tctcaaaaat agctaccctc tccggcatga atttatcagc tagaacggtt gaatatcata
     4621 ttgatggtga tttgactgtc tccggccttt ctcacccgtt tgaatcttta cctacacatt
     4681 actcaggcat tgcatttaaa atatatgagg gttctaaaaa tttttatcct tgcgttgaaa
     4741 taaaggcttc tcccgcaaaa gtattacagg gtcataatgt ttttggtaca accgatttag
     4801 ctttatgctc tgaggcttta ttgcttaatt ttgctaattc tttgccttgc ctgtatgatt
     4861 tattggatgt tggaatgccc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     4921 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     4981 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     5041 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     5101 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     5161 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5221 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5281 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5341 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5401 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5461 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5521 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5581 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5641 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5701 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5761 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5821 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5881 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5941 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     6001 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     6061 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6121 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6181 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6241 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6301 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6361 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6421 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6481 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6541 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6601 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6661 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6721 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6781 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6841 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6901 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6961 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     7021 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     7081 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     7141 ccccgcgcgt tggccgattc attaatg
//
