LOCUS       CAG-GRN-HA              8267 bp    DNA     circular SYN 14-MAY-2024
DEFINITION  synthetic circular DNA
ACCESSION   pVec1
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 22, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        278..657
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        660..935
                     /label=chicken beta-actin promoter
     CDS             1510..3289
                     /codon_start=1
                     /label=hGRN
                     /translation="MWTLVSWVALTAGLVAGTRCPDGQFCPVACCLDPGGASYSCCRPL
                     LDKWPTTLSRHLGGPCQVDAHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFH
                     CSADGRSCFQRSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVH
                     CCPHGAFCDLVHTRCITPTGTHPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCC
                     ELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGD
                     VKCDMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPH
                     QVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSD
                     HQHCCPQGYTCVAEGQCQRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCP
                     SLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQPATFLARSPHVGVK
                     DVECGEGHFCHDNQTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRRE
                     APRWDAPLRDPALRQLL"
     misc_feature    3307..3333
                     /label=HA
     misc_feature    3352..3940
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3823..3834)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4096..4151
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     rep_origin      5050..5563
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6310..6414
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6415..6483,6484..7275)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7446..8034
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
      121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca
      181 tgctctagcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat gagcttggcc
      241 cattgcatac gttgtatcca tatcataata tgtactcgac attgattatt gactagttat
      301 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      361 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      421 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      481 gactatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      541 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      601 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatgggt
      661 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
      721 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggc
      781 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg
      841 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
      901 ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgttg ccttcgcccc
      961 gtgccccgct ccgcgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc
     1021 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat
     1081 gacggctcgt ttcttttctg tggctgcgtg aaagccttaa agggctccgg gagggccctt
     1141 tgtgcggggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg
     1201 tgcggcccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc
     1261 tccgcgtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggggga cggctgcctt
     1321 cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc
     1381 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
     1441 gttgtgctgt ctcatcattt tggcaaagaa ttctcgagct caagcttcga attctgcagt
     1501 cgagccacca tgtggaccct ggtgagctgg gtggccttaa cagcagggct ggtggctgga
     1561 acgcggtgcc cagatggtca gttctgccct gtggcctgct gcctggaccc cggaggagcc
     1621 agctacagct gctgccgtcc ccttctggac aaatggccca caacactgag caggcatctg
     1681 ggtggcccct gccaggttga tgcccactgc tctgccggcc actcctgcat ctttaccgtc
     1741 tcagggactt ccagttgctg ccccttccca gaggccgtgg catgcgggga tggccatcac
     1801 tgctgcccac ggggcttcca ctgcagtgca gacgggcgat cctgcttcca aagatcaggt
     1861 aacaactccg tgggtgccat ccagtgccct gatagtcagt tcgaatgccc ggacttctcc
     1921 acgtgctgtg ttatggtcga tggctcctgg gggtgctgcc ccatgcccca ggcttcctgc
     1981 tgtgaagaca gggtgcactg ctgtccgcac ggtgccttct gcgacctggt tcacacccgc
     2041 tgcatcacac ccacgggcac ccaccccctg gcaaagaagc tccctgccca gaggactaac
     2101 agggcagtgg ccttgtccag ctcggtcatg tgtccggacg cacggtcccg gtgccctgat
     2161 ggttctacct gctgtgagct gcccagtggg aagtatggct gctgcccaat gcccaacgcc
     2221 acctgctgct ccgatcacct gcactgctgc ccccaagaca ctgtgtgtga cctgatccag
     2281 agtaagtgcc tctccaagga gaacgctacc acggacctcc tcactaagct gcctgcgcac
     2341 acagtggggg atgtgaaatg tgacatggag gtgagctgcc cagatggcta tacctgctgc
     2401 cgtctacagt cgggggcctg gggctgctgc ccttttaccc aggctgtgtg ctgtgaggac
     2461 cacatacact gctgtcccgc ggggtttacg tgtgacacgc agaagggtac ctgtgaacag
     2521 gggccccacc aggtgccctg gatggagaag gccccagctc acctcagcct gccagaccca
     2581 caagccttga agagagatgt cccctgtgat aatgtcagca gctgtccctc ctccgatacc
     2641 tgctgccaac tcacgtctgg ggagtggggc tgctgtccaa tcccagaggc tgtctgctgc
     2701 tcggaccacc agcactgctg cccccagggc tacacgtgtg tagctgaggg gcagtgtcag
     2761 cgaggaagcg agatcgtggc tggactggag aagatgcctg cccgccgggc ttccttatcc
     2821 caccccagag acatcggctg tgaccagcac accagctgcc cggtggggca gacctgctgc
     2881 ccgagcctgg gtgggagctg ggcctgctgc cagttgcccc atgctgtgtg ctgcgaggat
     2941 cgccagcact gctgcccggc tggctacacc tgcaacgtga aggctcgatc ctgcgagaag
     3001 gaagtggtct ctgcccagcc tgccaccttc ctggcccgta gccctcacgt gggtgtgaag
     3061 gacgtggagt gtggggaagg acacttctgc catgataacc agacctgctg ccgagacaac
     3121 cgacagggct gggcctgctg tccctaccgc cagggcgtct gttgtgctga tcggcgccac
     3181 tgctgtcctg ctggcttccg ctgcgcagcc aggggtacca agtgtttgcg cagggaggcc
     3241 ccgcgctggg acgccccttt gagggaccca gccttgagac agctgctgac gcgtacgcgg
     3301 ccgctctacc catacgatgt tccagattac gcttaaatcg gtaccggatc caatcaacct
     3361 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     3421 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     3481 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     3541 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     3601 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     3661 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     3721 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     3781 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     3841 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     3901 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggatccaa ttcactcctc
     3961 aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat
     4021 accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     4081 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     4141 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     4201 atttggttta gagtttggca acatatgcca tatgctggct gccatgaaca aaggtggcta
     4261 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
     4321 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca
     4381 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
     4441 ccagtcatag ctgtccctct tctcttatga agatccctcg acctgcagcc caagctctag
     4501 agcatggcta cgtagataag tagcatggcg ggttaatcat taactacaag gaacccctag
     4561 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
     4621 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgccagc
     4681 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     4741 ggcgaatggc gattctgttg caatggctgg cggtaatatt gttctggata ttaccagcaa
     4801 ggccgatagt ttgagttctt ctactcaggc aagtgatgtt attactaatc aaagaagtat
     4861 tgcgacaacg gttaatttgc gtgatggaca gactctttta ctcggtggcc tcactgatta
     4921 taaaaacact tctcaggatt ctggcgtacc gttcctgtct aaaatccctt taatcggcct
     4981 cctgtttagc tcccgctctg attctaacga ggaaagcacg ttatacgtgc tcgtcaaagc
     5041 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     5101 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     5161 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     5221 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     5281 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     5341 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     5401 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     5461 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttaaata tttgcttata
     5521 caatcttcct gtttttgggg cttttctgat tatcaaccgg ggtacatatg attgacatgc
     5581 tagttttacg attaccgttc atcgattctc ttgtttgctc cagactctca ggcaatgacc
     5641 tgatagcctt tgtagagacc tctcaaaaat agctaccctc tccggcatga atttatcagc
     5701 tagaacggtt gaatatcata ttgatggtga tttgactgtc tccggccttt ctcacccgtt
     5761 tgaatcttta cctacacatt actcaggcat tgcatttaaa atatatgagg gttctaaaaa
     5821 tttttatcct tgcgttgaaa taaaggcttc tcccgcaaaa gtattacagg gtcataatgt
     5881 ttttggtaca accgatttag ctttatgctc tgaggcttta ttgcttaatt ttgctaattc
     5941 tttgccttgc ctgtatgatt tattggatgt tggaatgccc tgatgcggta ttttctcctt
     6001 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     6061 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     6121 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     6181 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     6241 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     6301 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6361 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     6421 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     6481 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     6541 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     6601 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     6661 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     6721 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     6781 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     6841 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     6901 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     6961 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7021 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7081 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7141 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     7201 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     7261 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     7321 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     7381 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7441 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7501 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7561 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     7621 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7681 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7741 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7801 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7861 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7921 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7981 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8041 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8101 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     8161 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     8221 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatg
//
