LOCUS       mCherry_-_4S9            885 bp ds-DNA     linear       27-JUL-2023
DEFINITION  .
COMMENT     This sequence was created by back translating and codon optimizing
            the following AA sequence from AA 1 to AA 294:   The sequence was
            optimized for the organism E.coli. Optimization Results: The
            optimized number of rare codons was 3. The optimized GC content was
            0.5. The optimized thymine content was 0.21. The optimized number
            of hairpins was 0.
FEATURES             Location/Qualifiers
     CDS             1..885
                     /label="Translation 1-885"
                     /translation="MGSSMVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKGGSGGSGGSLPESGGGSGGSGGSRGVPHIVMVDAYKRYKGGSGGSGGSHHHHHH*"
     misc_feature    13..720
                     /label="mCherry "
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    748..762
                     /label="4S9"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    790..837
                     /label="SpyTag003"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    865..882
                     /label="6xHis Tag"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 ATGGGGAGTT CAATGGTGTC CAAAGGGGAA GAGGACAATA TGGCTATCAT TAAAGAATTT
       61 ATGCGTTTCA AAGTACATAT GGAAGGAAGC GTTAACGGTC ACGAATTCGA GATCGAAGGC
      121 GAAGGCGAGG GTCGCCCATA CGAAGGTACG CAGACCGCTA AACTGAAAGT AACCAAAGGC
      181 GGACCTTTAC CCTTCGCCTG GGACATTCTA AGTCCGCAAT TTATGTATGG TAGCAAGGCG
      241 TACGTTAAAC ACCCGGCAGA TATCCCAGAC TATTTAAAGT TGTCGTTTCC TGAGGGTTTT
      301 AAATGGGAGC GAGTGATGAA CTTCGAGGAT GGGGGCGTGG TCACAGTCAC GCAGGATTCG
      361 TCGCTGCAGG ATGGGGAATT TATTTATAAA GTCAAACTGC GCGGGACTAA CTTTCCGTCG
      421 GACGGCCCGG TCATGCAAAA AAAAACCATG GGTTGGGAAG CGTCTAGCGA ACGTATGTAC
      481 CCCGAAGATG GGGCATTGAA AGGGGAAATT AAACAACGTC TGAAACTCAA AGATGGCGGA
      541 CATTATGATG CGGAGGTGAA AACCACTTAT AAGGCGAAAA AACCAGTGCA GCTGCCGGGT
      601 GCATATAACG TTAATATTAA ACTTGACATC ACATCTCATA ATGAAGATTA TACCATAGTG
      661 GAACAGTACG AACGTGCCGA AGGTCGCCAC TCTACGGGCG GCATGGACGA GCTGTACAAG
      721 GGCGGTTCCG GCGGTAGTGG TGGAAGCCTG CCGGAAAGCG GCGGAGGTTC AGGAGGTTCC
      781 GGCGGCAGTC GGGGCGTACC GCATATTGTT ATGGTTGATG CCTATAAGCG CTACAAGGGT
      841 GGTTCCGGCG GCAGCGGCGG CTCACACCAT CATCACCATC ATTAA
//