LOCUS       mCerulean_-_TEV          900 bp ds-DNA     linear       25-AUG-2023
DEFINITION  .
COMMENT     This sequence was created by back translating and codon optimizing
            the following AA sequence from AA 1 to AA 299:   The sequence was
            optimized for the organism E.coli. Optimization Results: The
            optimized number of rare codons was 3. The optimized GC content was
            0.49. The optimized thymine content was 0.24. The optimized number
            of hairpins was 0.
FEATURES             Location/Qualifiers
     CDS             1..900
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MGSSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGSGGSGGSENLYFQSGGSGGSGGSRGVPHIVMVDAYKRYKGGSGGSGGSHHHHHH*"
     misc_feature    13..732
                     /label="mCerulean"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATGGGATCTT CGATGGTCTC CAAAGGCGAA GAACTGTTCA CGGGTGTGGT CCCGATTCTG
       61 GTGGAATTGG ATGGTGACGT AAATGGCCAT AAGTTTTCGG TGAGCGGCGA AGGCGAAGGC
      121 GATGCGACCT ATGGCAAGTT AACCCTGAAA TTCATCTGTA CCACGGGAAA ACTACCGGTG
      181 CCGTGGCCAA CGCTGGTGAC CACCCTGACG TGGGGCGTAC AGTGCTTTGC TCGTTATCCG
      241 GATCACATGA AACAGCACGA CTTCTTTAAA AGCGCTATGC CGGAGGGCTA CGTGCAGGAA
      301 CGGACAATCT TTTTTAAAGA CGATGGGAAT TATAAAACGC GTGCCGAAGT TAAATTTGAG
      361 GGTGATACTC TGGTTAATCG TATCGAACTG AAAGGGATCG ATTTCAAAGA GGATGGGAAC
      421 ATACTCGGTC ATAAATTGGA GTATAACGCG ATTTCGGATA ACGTTTATAT TACAGCAGAC
      481 AAACAGAAGA ACGGCATTAA GGCCAACTTT AAAATCCGCC ATAACATTGA AGATGGAAGC
      541 GTGCAGCTCG CGGATCACTA CCAACAAAAT ACCCCCATCG GCGACGGTCC TGTCCTGCTT
      601 CCTGATAATC ACTATTTAAG TACTCAAAGT AAACTTTCAA AGGACCCAAA CGAAAAACGC
      661 GACCACATGG TGTTGCTGGA GTTCGTCACC GCCGCGGGCA TTACTTTAGG TATGGATGAA
      721 CTGTACAAAG GTGGATCAGG TGGTTCCGGG GGCAGCGAAA ATCTGTACTT TCAGTCAGGC
      781 GGCTCCGGGG GGTCTGGAGG CAGCCGCGGT GTACCGCATA TTGTTATGGT TGATGCATAC
      841 AAGCGATATA AAGGTGGCAG TGGTGGGTCT GGTGGTAGCC ATCATCATCA CCATCATTAA
//