LOCUS       493-mitoGCAMP-7-m       7143 bp ds-DNA     circular     30-APR-2025
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            493-mitoGCAMP-7-m.annotation.gbk Annotated with pLannotate v1.2.1
            The mutations compared to GCaMP6f are K78H
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /label="ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             772..864
                     /label="lacI (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ*"
     protein_bind    877..898
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        913..943
                     /label="lac promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    951..967
                     /label="lac operator"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     polyA_signal    complement(1040..1161)
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(1156..1314)
                     /label="ble (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="98.1"
                     /match_length="42.1"
                     /fragment="True"
                     /other="CDS"
                     /translation="MTDQATPNLPSRDFDSTAAFYERLGFGIVFRDAGWMILQRGDLMLEFFAHPNL"
     CDS             complement(1338..2129)
                     /label="neo"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2196..2525)
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      complement(2539..2967)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(2988..3009)
                     /label="SV40 promoter (fragment)"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.1"
                     /fragment="True"
                     /other="promoter"
     polyA_signal    complement(3013..3237)
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(3287..3289)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="*"
     CDS             complement(3290..4636)
                     /label="GCaMP2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="fpbase"
                     /identity="92.9"
                     /match_length="99.6"
                     /fragment="False"
                     /other="CDS"
                     /translation="GSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNKTGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGIGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     misc_feature    3554..3556
                     /label="D362I"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3689..3691
                     /label="E317A"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4406..4408
                     /label="K78H"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(4637..4966)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="LGDPMSVLTPLLLRGLTGSARRLPVPRAKIHSLGDPMSVLTPLLLRGLTGSARRLPVPRAKIHSLGDPMSVLTPLLLRGLTGSARRLPVPRAKIHSLGDPRSASGKDPTM"
     CDS             complement(4964..5050)
                     /label="COX8A (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="42.0"
                     /fragment="True"
                     /other="CDS"
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAKIHSL"
     promoter        complement(5096..5114)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     LTR             complement(5130..5163)
                     /label="5' LTR (truncated) (fragment)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="97.1"
                     /match_length="18.8"
                     /fragment="True"
                     /other="LTR"
     promoter        complement(5159..5362)
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     enhancer        complement(5363..5742)
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     misc_feature    5897..5949
                     /label="bom (fragment)"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="37.6"
                     /fragment="True"
                     /other="misc_feature"
     promoter        6008..6112
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             6113..6973
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     1321 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     1381 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     1441 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     1501 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     1561 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     1621 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     1681 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     1741 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     1801 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     1861 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     1921 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     1981 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     2041 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     2101 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     2161 gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa
     2221 aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa
     2281 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     2341 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     2401 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     2461 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     2521 cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2581 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2641 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2701 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2761 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2821 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2881 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2941 cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt
     3001 ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
     3061 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
     3121 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
     3181 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
     3241 aggctgatca gcgggtttaa acgggcccga tatcgaattc tctagattac ttggcggtca
     3301 tcatctggac gaactcttcg tagttcactt ggccgtcgcc atcaatgtcg gcctcgcgaa
     3361 tcatctcgtc aacctcttca tccgtcagct tctctcccag attggtcatc acgtggcgca
     3421 gttcggcggc actgatgtag ccgtttccat ccttgtcaaa cactccgaag gcctcgcgga
     3481 tctcttcctc tgtatcgcgg tacttcatct tgcgggccat catggtcagg aactcgggga
     3541 aatcgatcgt gccAATgcca tcggcgtcca cctcgttgat catatcctgc agctcggctt
     3601 ctgttggatt ctggcccagg gagcgcatca cggttcccag ttctttggtg gtaatggtgc
     3661 cgtcgccgtc cttatcgaac aggctgaaGG Cctctttaaa ctcggcgatc tgttcctcgg
     3721 tcagctgatc cggcaggttg tactccaact tgtgtcccag gatgttgcca tcctctttga
     3781 aatcgatgcc cttcagctcg atgcggttga ccagggtatc gccctcgaac ttcacttcgg
     3841 cgcgtgtctt gtagttgccg tcgtccttga aaaagatggt gcgctcttgg atgtagccct
     3901 ctggcatggc gctcttgaag aaatcgtgct gcttcatatg gtcggggtag cgcgagaagc
     3961 actgcacgcc ataggtcagt gtggtaacca gtgttggcca cggcacgggc aatttgccgg
     4021 tggtgcagat gaacttcagg gtcagctttc cataggtggc gtcgccttcg ccttctccgc
     4081 taacgctgaa cttgtggccg ttcacgtcgc catccaattc caccagaatg ggcaccacgc
     4141 cggtgaacag ttcctcgcct ttggaaacca tcgagccgcc ggttccgcct ttgtacagct
     4201 catccatgcc gagggtaatt ccggcggctg tcacgaactc caacagcacc atgtgatcgc
     4261 gcttctcgtt ggggtccttg ctcagcttgc tctgcacgct caggtaatgg ttatcgggca
     4321 gcagcactgg gccatctccg attggggtgt tctgctggta gtggtaggcc agctgcactc
     4381 cgccatcctc gatgttgtgg cggatGTGga agttggcctt aatgccgttc ttctgcttgt
     4441 cggccttgat gtacacgttt tccaggctgc tcagacgtcc aatggcgcgc acggcatgtc
     4501 ctgtcttgtt ccacttgcgg cggctgctat ccaccatcgt ggccagatcc ttatcatcat
     4561 catcgtacag atcgcgtccc atctgctggc cgcctgtcat actggccatt ccgtggtgat
     4621 ggtggtggtg gctacccatg gtgggatctt ttccgctagc agatctgggg tcgccgagcg
     4681 agtgaatttt tgcacgaggc accggcagtc ggcgagcgga acctgtcagt cccctcagca
     4741 acaagggcgt gagcacactc atagggtcgc ccaaggagtg gatcttggcc cttggaacag
     4801 gcaatcggcg tgccgatccg gtcagtcctc gcaacagcaa tggagtcaac accgacatgg
     4861 gatcgcccag ggaatgaatc ttagcacgcg gaactggcag gcgtcgggca cttccagtga
     4921 gtccacgcaa caacagtggg gtcagcacgc tcattggatc tcccaggctg tgaatcttgg
     4981 cgcgtggcac tggcaaacga cgagctgatc ctgtcaggcc gcgcagcagc agtggtgtca
     5041 aaacactcat ggcggtctcg aggcggccgc ggatccgcta gccagcttgg gtctccctat
     5101 agtgagtcgt attaatttcg ataagccagt aagcagtggg ttctctagtt agccagagag
     5161 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
     5221 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
     5281 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
     5341 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
     5401 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     5461 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     5521 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     5581 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     5641 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     5701 ccgtaattga ttactattaa taactagtca ataatcaatg tcaacgcgta tatctggccc
     5761 gtacatcgcg aagcagcgca aaacgcctaa ccctaagcag attcttcatg caattgtcgg
     5821 tcaagccttg ccttgttgta gcttaaattt tgctcgcgca ctactcagcg acctccaaca
     5881 cacaagcagg gagcagatac tggcttaact atgcggcatc agagcagatt gtactgagag
     5941 tgcaccatag gggatcggga gatctcccga tccgtcgacg tcaggtggca cttttcgggg
     6001 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6061 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6121 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6181 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6241 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6301 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6361 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6421 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6481 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6541 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6601 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6661 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6721 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6781 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6841 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6901 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     6961 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7021 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7081 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7141 ttc
//