LOCUS       pYPQ166-N-Trex2-        8578 bp    DNA     circular SYN 11-SEP-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8578)
  AUTHORS   Aimee
  TITLE     Direct Submission
  JOURNAL   Exported Sep 11, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..138
                     /label=Spn promoter
                     /note="/ApEinfo_fwdcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=pink
                     /dnas_title=Spn promoter
                     /vntifkey=30"
     CDS             139..1149
                     /codon_start=1
                     /gene="Streptomycin/Spectinomycin adenyltransferase "
                     /label=SpnR
                     /note="/ApEinfo_fwdcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=pink
                     /dnas_title=SpnR
                     /invitrogen=2750000
                     /vntifkey=4"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1216..1889
                     /gene="ori"
                     /label=pUC origin
                     /note="/ApEinfo_fwdcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=pink
                     /dnas_title=pUC origin
                     /invitrogen=2530000
                     /vntifkey=33"
     terminator      2319..2362
                     /label=rrnB T1 terminatior
                     /note="/ApEinfo_fwdcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=pink
                     /dnas_title=rrnB T1 terminatior
                     /vntifkey=43"
     primer_bind     2429..2444
                     /label=M13 (-20) forward primer
     misc_recomb     2461..2560
                     /label=attL1
                     /note="/ApEinfo_fwdcolor=#00ff40
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=pink
                     /dnas_title=attL1
                     /invitrogen=2670000
                     /vntifkey=86"
     CDS             2581..3288
                     /codon_start=1
                     /label=TREX2
                     /translation="MSEAPRAETFVFLDLEATGLPSVEPEIAELSLFAVHRSSLENPEH
                     DESGALVLPRVLDKLTLCMCPERPFTAKASEITGLSSEGLARCRKAGFDGAVVRTLQAF
                     LSRQAGPICLVAHNGFDYDFPLLCAELRRLGARLPRDTVCLDTLPALRGLDRAHSHGTR
                     ARGRQGYSLGSLFHRYFRAEPSAAHSAEGDVHTLLLIFLHRAAELLAWADEQARGWAHI
                     EPMYLPPDDPSLEF"
     primer_bind     2927..2947
                     /label=Trex2-seq-F
     misc_feature    3289..3336
                     /label=Nucleoplasmin NLS
                     /note="Nucleoplasmin NLS"
     primer_bind     complement(3322..3337)
                     /label=Cas3-C-R1
     CDS             3337..3384
                     /codon_start=1
                     /label=XTEN linker
                     /note="XTEN linker"
                     /translation="SGSETPGTSESATPES"
     primer_bind     complement(3363..3424)
                     /label=Fragment.REV
     CDS             3385..7653
                     /codon_start=1
                     /label=zCas9 fusion protein
                     /label=zCas9
                     /note="/dnas_title=zCas9
                     /vntifkey=4"
                     /translation="DYKDHDGDYKDHDIDYKDDDDKMAPKKKRKVGIHGVPAADKKYSI
                     GLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKR
                     TARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDE
                     VAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKL
                     FIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIA
                     LSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILL
                     SDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAG
                     YIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELH
                     AILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFE
                     EVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPA
                     FLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDL
                     LKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRY
                     TGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSG
                     QGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQ
                     KNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRL
                     SDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLI
                     TQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIR
                     EVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVY
                     GDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGET
                     GEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDP
                     KKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGY
                     KEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLK
                     GSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQ
                     AENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQ
                     LGGDKRPAATKKAGQAKKKK"
     sig_peptide     3385..3450
                     /label=3xFLAG
     misc_feature    3457..3477
                     /label=SV40 NLS
     CDS             3502..7602
                     /codon_start=1
                     /label=zCas9
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    7603..7650
                     /label=nucleoplasmin NLS
     primer_bind     complement(7656..7689)
                     /label=Cas3-C-R1
     terminator      7658..8278
                     /label=rbcS-E9t
                     /note="/dnas_title=rbcS-E9t
                     /vntifkey=43"
     misc_recomb     8325..8448
                     /label=attR5
                     /note="/ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=pink
                     /dnas_title=attR5
                     /invitrogen=2703000
                     /vntifkey=86"
     primer_bind     complement(8489..8505)
                     /label=M13 reverse primer
     promoter        8577..8578
                     /label=Spn promoter
                     /note="/ApEinfo_fwdcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=pink
                     /dnas_title=Spn promoter
                     /vntifkey=30"
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttcaccat
     2581 atgtcagaag cacctcgtgc cgagaccttt gtcttcctgg acctggaagc cactgggctc
     2641 cccagtgtgg agcccgagat tgctgagctg tctctatttg ctgtccaccg ctcctccctg
     2701 gagaacccgg agcacgacga gtctggtgca ctagtattgc cacgggtcct tgacaagctt
     2761 acgctgtgca tgtgccctga gcgccccttc actgctaagg ctagcgagat caccggactg
     2821 agcagtgaag gcctggcgcg atgtcggaag gctggctttg atggcgcagt ggtgcggaca
     2881 ctgcaggcat tcctgagccg tcaggcaggg cccatctgtc ttgtggccca caatggattt
     2941 gattatgatt tcccactgct gtgtgccgag ctgcggcgac tgggtgctcg actgccccgg
     3001 gatactgtct gcctggacac tctgccggca ctgcgtggcc tggaccgtgc ccacagtcac
     3061 ggcacccggg cacggggtcg ccagggttac agtctcggca gtctcttcca tcgctacttc
     3121 cgagcagagc caagcgcagc ccattcagct gagggcgacg tgcataccct gctactgatc
     3181 ttcctgcatc gcgccgcaga gctactcgcc tgggcagatg agcaagcccg tggatgggct
     3241 cacatcgagc ccatgtactt accgcctgat gatcccagcc tggagtttaa acgtcctgct
     3301 gccaccaaaa aggccggaca ggctaagaaa aagaagtcag gttctgagac ccctggcaca
     3361 agtgagtcag caacacccga gtccgattac aaggaccacg acggggatta caaggaccac
     3421 gacattgatt acaaggatga tgatgacaag atggctccga agaagaagag gaaggttggc
     3481 atccacgggg tgccagctgc tgacaagaag tactcgatcg gcctcgatat tgggactaac
     3541 tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa gttcaaggtc
     3601 ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct cctgttcgac
     3661 agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg gtacacgcgc
     3721 aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc gaaggttgac
     3781 gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa gaagcacgag
     3841 cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa gtaccctacg
     3901 atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct ccgcctgatc
     3961 tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga gggggatctc
     4021 aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac gtacaaccag
     4081 ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat cctgtccgct
     4141 aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg cgagaagaag
     4201 aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa cttcaagtcg
     4261 aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata cgacgatgac
     4321 ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct cgctgccaag
     4381 aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga gattacgaag
     4441 gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga tctgaccctc
     4501 ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt cttcgatcag
     4561 tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga gttctacaag
     4621 ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt gaagctcaat
     4681 cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc acaccagatt
     4741 catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc tttcctcaag
     4801 gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta cgtcggccca
     4861 ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga gacgatcacg
     4921 ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt catcgagagg
     4981 atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca ctcgctcctg
     5041 tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac cgagggcatg
     5101 aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct cctgttcaag
     5161 accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa gattgagtgc
     5221 ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct ggggacctac
     5281 cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga gaatgaggat
     5341 atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat gatcgaggag
     5401 cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct caagaggagg
     5461 aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg ggacaagcag
     5521 tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg caatttcatg
     5581 cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc tcaggtgagc
     5641 ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc agctatcaag
     5701 aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat gggcaggcac
     5761 aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca gaagggccag
     5821 aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct ggggtcccag
     5881 atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct ctacctgtac
     5941 tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa caggctcagc
     6001 gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc cattgacaac
     6061 aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc ttcagaggag
     6121 gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct gatcacgcag
     6181 cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct ggacaaggcg
     6241 ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt tgcgcagatt
     6301 ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg cgaggtgaag
     6361 gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca gttctacaag
     6421 gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc ggtggtcggc
     6481 acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg ggactacaag
     6541 gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa ggctaccgcc
     6601 aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac tctggccaat
     6661 ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga gatcgtgtgg
     6721 gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca agtgaatatc
     6781 gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct gcctaagcgg
     6841 aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta cggcgggttc
     6901 gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa gggcaagtcc
     6961 aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg ctccagcttc
     7021 gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa gaaggacctg
     7081 atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa gcggatgctg
     7141 gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa gtatgtgaac
     7201 ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga caacgagcag
     7261 aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga gcagatttcc
     7321 gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct ctccgcgtac
     7381 aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca tctcttcacc
     7441 ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat cgatcgcaag
     7501 aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc gattaccggc
     7561 ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc agcggcgacg
     7621 aagaaggcgg ggcaggcgaa gaagaagaag tgagctcaga gctttcgttc gtatcatcgg
     7681 tttcgacaac gttcgtcaag ttcaatgcat cagtttcatt gcgcacacac cagaatccta
     7741 ctgagtttga gtattatggc attgggaaaa ctgtttttct tgtaccattt gttgtgcttg
     7801 taatttactg tgttttttat tcggttttcg ctatcgaact gtgaaatgga aatggatgga
     7861 gaagagttaa tgaatgatat ggtccttttg ttcattctca aattaatatt atttgttttt
     7921 tctcttattt gttgtgtgtt gaatttgaaa ttataagaga tatgcaaaca ttttgttttg
     7981 agtaaaaatg tgtcaaatcg tggcctctaa tgaccgaagt taatatgagg agtaaaacac
     8041 ttgtagttgt accattatgc ttattcacta ggcaacaaat atattttcag acctagaaaa
     8101 gctgcaaatg ttactgaata caagtatgtc ctcttgtgtt ttagacattt atgaactttc
     8161 ctttatgtaa ttttccagaa tccttgtcag attctaatca ttgctttata attatagtta
     8221 tactcatgga tttgtagttg agtatgaaaa tattttttaa tgcattttat gacttgccaa
     8281 ttgttccgga actctagata agcttaccgg aaagggcgaa ttcgcaactt tgtatacaaa
     8341 agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     8401 aaaacagact acataatact gtaaaacaca acatatccag tcactatgcc atccagctga
     8461 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     8521 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctc
//
