LOCUS       pYPQ230-N-AtExo1        9254 bp    DNA     circular SYN 11-APR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Aimee
  TITLE     Direct Submission
  JOURNAL   Exported Sep 11, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9254
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        1..138
                     /label=Spn promoter
     CDS             139..1149
                     /label=SpnR
     rep_origin      1216..1889
                     /direction=RIGHT
                     /label=pUC origin
     terminator      2319..2362
                     /label=rrnB T1 terminatior
     primer_bind     2429..2444
                     /label=M13 (-20) forward primer
     misc_recomb     2461..2543
                     /label=attL1
                     /note="/ApEinfo_fwdcolor=#00ff40 
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 
                     0/ApEinfo_revcolor=pink/dnas_title=attL1/invitrogen=2670000
                     /vntifkey=86"
     primer_bind     2535..2559
                     /label=AtExo1b-N-F
     misc_recomb     2544..2560
                     /label=attL1
                     /note="/ApEinfo_fwdcolor=#00ff40
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=pink
                     /dnas_title=attL1
                     /invitrogen=2670000
                     /vntifkey=86"
     primer_bind     2566..2582
                     /label=AtExo1b-N-LNK-F
     primer_bind     2581..2604
                     /label=Exo1b-F1
     misc_feature    2584..4789
                     /label=AtExo1b
     misc_feature    4792..4839
                     /label=Nucleoplasmin NLS
                     /note="Nucleoplasmin NLS"
     primer_bind     complement(4824..4850)
                     /label=AtExo1b-R2
     CDS             4846..4892
                     /codon_start=1
                     /label=XTEN linker
                     /note="XTEN linker"
                     /translation="SGSETPGTSESATPE"
     primer_bind     complement(4883..4897)
                     /label=AtExo1b-N-R
     gene            4894..4944
                     /label=N-terminal NLS
                     /note="N-terminal NLS"
     misc_feature    4900..4920
                     /label=SV40 NLS
                     /note="SV40 NLS"
     gene            4945..8625
                     /label=LbCpf1
                     /note="LbCpf1"
     primer_bind     4945..4970
                     /label=LbCpf1-NotI-F
     misc_feature    5372..5395
                     /label=LbCpf1-seq1
                     /note="""LbCpf1-seq1"
     primer_bind     complement(8601..8625)
                     /label=LbCpf1-NcoI-R
     misc_feature    8626..8673
                     /label=Nucleoplasmin NLS
                     /note="Nucleoplasmin NLS"
     primer_bind     complement(8658..8677)
                     /label=AtExo1b-R2
     misc_feature    8674..8676
                     /label=stop
                     /note="stop"
     misc_feature    8702..8979
                     /label=NOS term
     misc_feature    complement(8746..8769)
                     /label=Nos-term-R2
                     /note="Nos-term-R2"
     misc_recomb     9001..9124
                     /label=attR5
     primer_bind     9165..9181
                     /label=M13 reverse primer
     promoter        9253..9254
                     /label=Spn promoter
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ggtacgttcc ctaccggccg
     2581 catatgggta tacaagggct tttgccgttg ttgaaatcga taatggttcc gattcatatc
     2641 aaggaactcg agggttgtat cgtcgccgtc gatacatatt catggctaca caaaggggct
     2701 ttatcttgta gccgagaact ctgcaaggga ttacccacca agaggcatat tcaatactgt
     2761 atgcatagag ttaatttact tcgccatcat ggagttaagc ctatcatggt ctttgatgga
     2821 ggtccgttac cgatgaaact agaacaagag aataagcgtg ctaggtccag aaaggaaaat
     2881 cttgctcgtg cattggagca tgaagcaaat ggaaattcct cagctgctta tgagtgctac
     2941 tcaaaggccg tagatatttc accttctatt gcacatgagt tgatacaagt tctgaggcag
     3001 gagaatgttg attacgttgt tgctccttac gaagctgatg cgcagatggc attcttagct
     3061 attactaagc aagttgatgc gattattact gaggattctg atctcatacc ttttggctgc
     3121 ctaagaatca ttttcaaaat ggacaagttt ggacacggtg ttgagtttca agcctccaag
     3181 ctacccaaaa ataaagatct cagtctatca ggattttcaa gccaaatgct tctcgaaatg
     3241 tgcatattaa gtggctgcga ttatttgcag tctcttccag gaatgggact caaaagagca
     3301 catgcactca ttacaaaatt caaaagctat gacagggtaa ttaagcactt aaagtacagc
     3361 acagtttcag ttcctcctct ttatgaagag tcattcaaga gagctttact aactttcaag
     3421 catcaacgtg tctatgaccc aaatgctgaa gatatcatac acttgtgtga catttctgat
     3481 aaccttggtg aagattcaga ttttgtaggc ccatcgatgc cacaagatat tgctaagggt
     3541 atagctctag gccagcttga tccattcaca cagttgccat tccaggctga gagtgttact
     3601 cctaaattgg ctgttgatga tatatcccgt cccaaaagtt tcaaacctga aactgtaaag
     3661 aaaaagcttg atttgccagt ccaaaaaaac cttctcacca agtacttctg ctttgcgtct
     3721 gttgaggcaa aaagaaaatt caaggctcct aggatatcac caatgtctct taccccaact
     3781 gatgagtctc caagcattcc tgatgacaac acaccagatt tagatgctct ttcaagccag
     3841 acaacaaatg aatccccggt ttatagcttg ggtgagaatc catgtgttag cgaagttgca
     3901 gagaaaagag attcacccga tgacgatgca gtggaaagga atcacaagga ccttcatcat
     3961 aaatactgcg aacgggaagt ggataggccg aaatcagata gtttgaaggt tatagtaaga
     4021 agcaagtatt ttaagcagaa acaggaggac aaaagtctga aacaatctat cccatgtctt
     4081 aatgattgct ctgtgattgg tcaaagaaag gctgttaaaa ctgtgattaa catgtcaagc
     4141 gcgtctaaaa gagaagagag tcacagagct atagcaacaa gtccatgttt acatcatgac
     4201 cgaatctaca acgatcatga agatgctaaa gaagcgagtt tctctgccat gaacgaggtg
     4261 gctgagagaa caataaacac ccacaagatt aaccatcaga tcaatgaaga agaacaaaac
     4321 ccatccgttg aaattccttc tgcctttagt actcccgaga atgttatacc tctctcttcc
     4381 attgctattg atagttgcca cggagttgca acagggaaga ggaagcttga ctcagatgaa
     4441 aaccttcaca aggaaaattt gaaatccaag cacatgcgca tggatgaaac tgatactgct
     4501 cttaatgcag aaacaccatt agaaaccgat gatgttgaga agtttggttc gaacatttca
     4561 catatagggc attactcaga aatagcagag aaatcagtgg aaagatttgt ctcagctata
     4621 tcgtctttca aatattctgg gactggctct cgtgctagcg gtctccgtgc ccctctcaaa
     4681 gatatccgca acacttgtcc gtccaaaggg ttatctctta aaccagacat cagcaagttt
     4741 ggctatgcgt caagcaaccg acacatggtg acaaagtcaa ggagaatgtt taaacgtcct
     4801 gctgccacca aaaaggccgg acaggctaag aaaaagaagg agctctcagg atctgagacc
     4861 ccaggcacaa gtgaatcagc tacacctgag tccatggctc ctaagaagaa gcggaaggtt
     4921 ggtattcacg gggtgcctgc ggcttcaaag ctcgagaaat tcaccaactg ttattcgttg
     4981 agcaaaacac tgcggtttaa agcgattcca gtcggcaaga ctcaagagaa tatagacaat
     5041 aagcggctgt tggtggaaga tgaaaagcgc gcggaagact acaaaggggt gaagaagttg
     5101 ttggacagat actacctctc ttttatcaat gatgtcttgc actcaatcaa attgaagaat
     5161 ctgaacaact acatctccct cttcagaaag aaaacaagga cagaaaagga gaataaggaa
     5221 cttgaaaatt tggagatcaa tctgaggaaa gagatcgcga aagcctttaa aggcaacgaa
     5281 ggatacaaaa gtctgttcaa gaaggatata attgagacaa ttttgccaga gttcctcgat
     5341 gacaaggacg agattgcgct ggtcaattcg ttcaacggat tcacaacagc attcacaggc
     5401 ttctttgata atcgggaaaa tatgttctct gaggaggcaa agtccacttc tattgcgttc
     5461 aggtgtatca atgagaatct cactaggtac atttccaaca tggatatctt tgagaaggtt
     5521 gacgcaattt ttgacaagca cgaagttcag gagattaagg agaagatcct caattccgat
     5581 tatgacgttg aggacttctt cgaaggtgag ttttttaatt tcgtgctcac tcaagagggt
     5641 atcgacgtgt ataatgcgat catcggtggg ttcgtgactg agtccggtga aaagattaag
     5701 ggattgaacg agtatatcaa cctttacaac caaaagacga aacagaagct gccaaagttc
     5761 aagcctcttt acaaacaggt tctttcagac cgcgagtcac tctcgttcta tggggagggc
     5821 tacacttcgg atgaggaagt cctggaggtg ttcaggaata ctctcaataa gaattcggag
     5881 attttctctt ctataaaaaa actggaaaag ttgtttaaga attttgacga atactctagc
     5941 gccggcatat ttgtgaaaaa cggcccggcc atatcaacga taagtaaaga tatcttcggc
     6001 gaatggaacg tgatcagaga caaatggaac gcggagtatg acgatattca cctgaagaag
     6061 aaggctgtcg taacggagaa gtacgaggat gatcgcagga aaagcttcaa aaagatcgga
     6121 agtttcagcc tggaacagtt gcaggagtat gctgacgccg atcttagcgt cgtcgagaag
     6181 ttgaaggaga taatcatcca aaaggtcgac gagatatata aagtctatgg atcaagtgaa
     6241 aaactgttcg acgccgactt cgttttggag aagtccctga agaagaacga cgctgttgtt
     6301 gccattatga aggatctgct cgacagcgtg aagagtttcg agaactatat taaggctttt
     6361 ttcggggagg ggaaggagac taacagagat gagtccttct acggagactt cgtcctcgcg
     6421 tacgatatac tccttaaggt agaccacatc tacgacgcaa tcagaaatta cgtgacacaa
     6481 aagccgtaca gcaaggacaa gttcaaactc tacttccaga acccccagtt catgggcggc
     6541 tgggacaagg acaaggaaac ggattacagg gctacgatcc tgaggtatgg ttcaaaatac
     6601 tacttggcga ttatggacaa gaagtacgcc aagtgtctcc agaagattga caaagacgat
     6661 gtcaatggca attatgagaa gatcaactac aagctgcttc cgggtccgaa caagatgctc
     6721 ccaaaggttt tcttcagcaa gaaatggatg gcctactata acccaagcga ggacatccag
     6781 aagatttata agaacggtac gttcaagaag ggcgacatgt tcaatcttaa cgactgtcac
     6841 aagctgatcg acttcttcaa agactcaatt agccggtacc caaagtggtc taacgcctat
     6901 gacttcaact tttcggaaac cgagaagtac aaggatatag ccggatttta tagagaggtg
     6961 gaagagcagg gctacaaggt gtcattcgag tccgccagca agaaggaagt ggacaagctc
     7021 gtggaagagg gtaagctcta catgttccag atttataata aagactttag cgataagagc
     7081 cacgggacac ctaatctcca cacaatgtat ttcaagctgc tcttcgacga gaataaccac
     7141 ggccaaatca ggttgtcagg aggggctgaa ctcttcatgc ggcgcgctag ccttaagaag
     7201 gaggagcttg tagtccaccc tgcgaatagt ccaattgcga ataagaaccc ggacaatcct
     7261 aaaaagacta caacattgag ctacgacgtg tacaaggata agaggttttc cgaggatcag
     7321 tacgagctcc acatcccgat tgcgatcaac aagtgcccaa agaatatttt caagataaac
     7381 acagaggtgc gtgtactcct gaagcatgac gacaatcctt acgtcattgg gattgatcgg
     7441 ggcgagagga acctcctcta tattgtggtg gtggacggga aggggaacat agtcgaacag
     7501 tactccctta acgaaataat taacaatttc aacggcatcc gtatcaagac cgactaccat
     7561 tcgttgctgg acaagaagga gaaggagaga tttgaggcgc ggcaaaattg gacaagtatc
     7621 gagaacatca aggaactcaa agcaggttat atctctcaag ttgtgcataa gatatgcgag
     7681 ctggttgaga agtatgacgc agtgatcgct cttgaggacc tcaactcggg ctttaagaat
     7741 tctagagtta aagtggagaa gcaggtctat caaaagttcg agaagatgct tatagataag
     7801 ctcaactaca tggtcgataa gaaatcgaac ccatgtgcca ccggcggcgc actcaaaggt
     7861 taccaaataa caaacaaatt cgagtccttc aaatcgatga gtactcagaa tgggttcata
     7921 ttttatatac cggcgtggct tacgtctaag atcgacccgt caactggttt tgtcaacctg
     7981 ttgaagacga aatacacgtc cattgccgat tcgaaaaagt tcatatctag ttttgatcgt
     8041 attatgtacg tcccagagga agatcttttc gagtttgctc tcgactacaa aaacttttcg
     8101 cggaccgatg cggattacat taaaaaatgg aaactctatt cgtacggcaa cagaatcagg
     8161 atttttcgca accctaagaa gaataacgtc tttgattggg aggaagtttg cttgactagc
     8221 gcgtacaagg agctctttaa taagtatggc attaactacc aacagggtga tatcagagca
     8281 ctgctttgcg aacaatctga caaggctttc tactcatcct tcatggcttt gatgagcctg
     8341 atgctccaga tgagaaattc aattacaggc agaaccgacg tggatttctt gatctccccg
     8401 gttaaaaatt ctgatggcat cttttacgat agcaggaact atgaagcgca agagaatgcg
     8461 attctgccaa aaaatgcaga cgccaacggt gcctataaca tcgccaggaa agtcctgtgg
     8521 gcgatcggcc agttcaaaaa ggccgaagac gaaaaattgg acaaggtcaa aatcgctatc
     8581 agcaacaaag agtggctgga gtatgctcag acatccgtaa agcataagcg tcctgctgcc
     8641 accaaaaagg ccggacaggc taagaaaaag aagtgagacg actagtggcg gccgccgacg
     8701 tccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     8761 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     8821 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     8881 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     8941 tctatgttac tagatcggga attgatcccc cctcgacagc ttccggaaag ggcgaattcg
     9001 caactttgta tacaaaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     9061 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     9121 tatgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     9181 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     9241 catcatgcct cctc
//
