LOCUS       pNtVimentin-mche        7289 bp    DNA     circular SYN 16-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Alessandra Affolter Lab
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2025 from SnapGene 8.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     223..263
                     /label=testcmvpromfor
     promoter        233..699
                     /label=Pcmv/2xTetO2
                     /note="/vntifkey=29"
     promoter        769..958
                     /label=Pcmv/2xTetO2
                     /label=Pcmv/2xTetO2(1)
                     /note="/vntifkey=29"
     primer_bind     769..789
                     /label=CMV-for
     primer_bind     769..789
                     /label=CMVfor
     primer_bind     994..1022
                     /label=ntvimentinfor
     CDS             1005..2399
                     /codon_start=1
                     /label=mmVimentin
                     /note="mmVimentin"
                     /translation="STRSVSSSSYRRMFGGSGTSSRPSSNRSYVTTSTRTYSLGSALRP
                     STSRSLYSSSPGGAYVTRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKV
                     ELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQL
                     TNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAESTLQSFRQDVDNASLARLDLERK
                     VESLQEEIAFLKKLHDEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAK
                     NLQEAEEWYKSKFADLSEAANRNNDALRQAKQESNEYRRQVQSLTCEVDALKGTNESLE
                     RQMREMEENFALEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATY
                     RKLLEGEESRISLPLPTFSSLNLRETNLESLPLVDTHSKRTLLIKTVETRDGQVINETS
                     QHHDDLE"
     primer_bind     complement(2379..2406)
                     /label=ntvimentinrev
     primer_bind     2400..2426
                     /label=mTFPmito_for
     misc_feature    2406..2408
                     /label=GLy linker
     CDS             2409..3113
                     /codon_start=1
                     /label=mCherry
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     2409..2426
                     /label=testtfprab5cmvfor
     primer_bind     2409..2423
                     /label=mcherry5pletm1_for
     primer_bind     3047..3062
                     /label=mcherry for
     primer_bind     complement(3085..3113)
                     /label=mtfprab5f.REV
     primer_bind     complement(3092..3112)
                     /label=testtfprabcmvrev
     primer_bind     complement(3095..3137)
                     /label=mTFPmito_rev
     primer_bind     complement(3099..3133)
                     /label=mcherry3pletm1_reva
     primer_bind     complement(3099..3133)
                     /label=mcherry3pletm1_rev
     misc_feature    3114..3122
                     /label=Htt peptide 2
     misc_feature    3159..3161
                     /label=ATG
     primer_bind     3174..3201
                     /label=alfaformito_for
     primer_bind     3185..3198
                     /label=metalfa_for
     CDS             3186..3230
                     /codon_start=1
                     /label=ALFA
                     /note="ALFA"
                     /translation="PSRLEEELRRRLTEP"
     primer_bind     3186..3198
                     /label=testalfahorh2b_for
     primer_bind     complement(3214..3235)
                     /label=alfaforh2b_rev2
     primer_bind     complement(3215..3238)
                     /label=alfaformito_rev
     primer_bind     complement(3216..3233)
                     /label=alfaforh2b_rev
     misc_feature    3239..3241
                     /label=stop
     primer_bind     complement(3300..3317)
                     /label=BGH-rev
     polyA_site      3306..3530
                     /label=BGHpA
                     /note="/vntifkey=26"
     CDS             4461..4835
                     /label=ZeocinR
                     /note="/vntifkey=4"
     primer_bind     complement(4667..4685)
                     /label=testtfprabcmvrev
     primer_bind     complement(4674..4685)
                     /label=mcherry3pletm1_reva
     primer_bind     complement(4674..4685)
                     /label=mcherry3pletm1_rev
     primer_bind     complement(4965..4982)
                     /label=EGFP-C-Rev
     CDS             complement(6293..7153)
                     /label=AmpR
                     /note="/vntifkey=4"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc catgtctacc aggtctgtgt
     1021 cctcgtcctc ctaccgcagg atgttcggtg gctccggcac atcgagccgg cccagctcca
     1081 accggagcta tgtgaccacg tccacacgca cctacagtct gggcagcgca ctgcgcccca
     1141 gcactagccg cagcctctat tcctcatccc ccggtggcgc ctatgtgacc cggtcctcgg
     1201 cagtgcgcct gcggagcagc gtgccgggcg tgcggctgct tcaagactcg gtggacttct
     1261 cgctggccga cgccatcaac actgagttca agaacacccg caccaacgag aaggtagaac
     1321 tgcaggagct gaatgaccgc tttgccaact acatcgacaa ggtgcgcttc ctcgagcagc
     1381 agaacaaaat cctgctggct gagctcgagc agctcaaggg ccagggcaag tcgcgcctgg
     1441 gcgacctgta cgaggaggag atgcgggagc tgcgccggca ggtggatcag ctcaccaacg
     1501 acaaggcccg tgtcgaggtg gagcgggaca acctggccga ggacatcatg cggctgcgag
     1561 agaaattgca ggaggagatg ctccagagag aggaagccga aagcaccctg cagtcattca
     1621 gacaggatgt tgacaatgct tctctggcac gtcttgacct tgaacggaaa gtggaatcct
     1681 tgcaggaaga aattgccttt ttgaagaaac tgcacgatga agagatccag gagctgcagg
     1741 cccagattca ggaacagcat gtccagatcg atgtggacgt ttccaagcct gacctcactg
     1801 ctgccctgcg tgatgtgcgc cagcagtatg aaagcgtggc tgccaagaac ctccaggagg
     1861 ccgaggaatg gtacaagtcc aagtttgctg acctctctga ggctgccaac cggaacaacg
     1921 atgccctgcg ccaggccaag caggagtcaa acgagtaccg gagacaggtg cagtcactca
     1981 cctgtgaagt ggatgccctt aaaggcacta acgagtccct ggagcgccag atgcgtgaga
     2041 tggaagagaa ttttgccctt gaagctgcta actaccagga cactattggc cgcctgcagg
     2101 atgagatcca aaacatgaag gaagagatgg ctcgtcacct tcgtgaatac caagatctgc
     2161 tcaatgttaa gatggccctg gacattgaga tcgccaccta caggaagctg ctggaaggcg
     2221 aggagagcag gatttctctg cctctgccaa ccttttcttc cctgaacctg agagaaacta
     2281 acctggagtc acttcctctg gttgacaccc actcaaaaag aacactcctg attaagacgg
     2341 ttgagaccag agatggacag gtgatcaatg agacttctca gcatcacgat gaccttgaag
     2401 aattcggtgt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
     2461 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     2521 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
     2581 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
     2641 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     2701 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
     2761 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     2821 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     2881 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
     2941 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     3001 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     3061 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagggtggtg
     3121 gtggcgcggc cgcagatcga tcgatcacgc gtgccaccat gactagtttt gtacaaagcg
     3181 gtccgccatc acgtttggaa gaggaactga gacgccgctt aactgaacct ggccgcggta
     3241 aacatatgga tcgatcataa tactcgagtc tagagggccc gtttaaaccc gctgatcagc
     3301 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     3361 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     3421 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     3481 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     3541 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     3601 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     3661 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3721 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3781 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     3841 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     3901 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     3961 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     4021 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4081 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4141 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4201 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4261 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     4321 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     4381 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     4441 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
     4501 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
     4561 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
     4621 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
     4681 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
     4741 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
     4801 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
     4861 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     4921 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     4981 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5041 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     5101 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     5161 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     5221 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     5281 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     5341 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     5401 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     5461 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     5521 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     5581 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     5641 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5701 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     5761 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5821 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5881 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     5941 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     6001 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6061 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6121 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6181 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6241 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     6301 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     6361 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     6421 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     6481 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     6541 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     6601 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     6661 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     6721 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     6781 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     6841 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     6901 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     6961 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7021 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7081 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7141 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7201 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7261 catttccccg aaaagtgcca cctgacgtc
//
