LOCUS       pUASTattBRok-CIB       10363 bp    DNA     circular SYN 24-AUG-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10363)
  AUTHORS   Affolter Lab
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2025 from SnapGene 8.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10363
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      21..327
                     /label=F1 ori
     CDS             complement(460..528)
                     /label=LacZ alpha
                     /label=LacZ alpha(1)
     CDS             complement(460..528)
                     /label=LacZ alpha
     primer_bind     548..567
                     /label=pUCM13-52
     primer_bind     580..596
                     /label=M13-40
     primer_bind     599..616
                     /label=M13-fwd
     primer_bind     599..615
                     /label=M13
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     615..642
                     /label=T7
     primer_bind     622..642
                     /label=T7
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     623..641
                     /label=T7
     misc_feature    666..986
                     /label=w promoter
     primer_bind     856..875
                     /label=PBac to mw
     misc_feature    928..933
                     /label=Kozak
     primer_bind     complement(952..967)
                     /label=miniwhite 5' out
     misc_feature    987..4165
                     /label=mini-white
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3218..3349,
                     3420..4034)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This ia a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    1692..1697
                     /label=Kozak
     primer_bind     2662..2685
                     /label=mw 3' out
     primer_bind     complement(2728..2747)
                     /label=mw intern to 5'
     misc_feature    2842..2847
                     /label=Kozak
     misc_feature    2899..2904
                     /label=Kozak
     primer_bind     3474..3495
                     /label=white 3' out
     misc_feature    3623..3628
                     /label=Kozak
     primer_bind     3728..3749
                     /label=delWARI rev
     primer_bind     3826..3845
                     /label=WARIdel seq
     misc_feature    4023..4045
                     /label=New Feature
     misc_feature    4166..4780
                     /label=WARI
     primer_bind     4235..4256
                     /label=WARI out #1
     primer_bind     complement(4327..4349)
                     /label=WARI in #2
     primer_bind     4622..4644
                     /label=WARI out #2
     primer_bind     complement(4663..4684)
                     /label=WARI in #1
     misc_feature    4781..4803
                     /label=Forward primer to check insertions
                     /label=Forward primer to check insertions (rest of WARI)
     primer_bind     4781..4803
                     /label=pattBZ for
     protein_bind    complement(4809..4842)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_recomb     complement(4809..4842)
                     /label=LoxP
     protein_bind    4875..4969
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     misc_feature    4875..4890
                     /label=UAS
     misc_feature    4894..4909
                     /label=UAS
     misc_feature    4913..4928
                     /label=UAS
     misc_feature    4932..4947
                     /label=UAS
     misc_feature    4951..4966
                     /label=UAS
     promoter        4988..5226
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     5050..5059
                     /label=ap-5000AgeI_rev
     primer_bind     5174..5191
                     /label=UAS-1
     primer_bind     5174..5191
                     /label=forwardUAS(uas-1)
     CDS             5249..6596
                     /codon_start=1
                     /label=RhoK_conservative
                     /translation="MPAGRETVTKQRSMDVERRRRANTLEREMRDPTSICNVDCLLDTV
                     SALVSDCDHESLRRLKNIEQYAAKYKPLAMQINQLRMNVEDFHFIKLIGAGAFGEVQLV
                     RHKSSSQVYAMKRLSKFEMMKRPDSAFFWEERHIMAHANSEWIVQLHFAFQDAKYLYMV
                     MDFMPGGDIVSLMGDYDIPEKWAIFYTMEVVLALDTIHNMGFVHRDVKPDNMLLDSYGH
                     LKLADFGTCMRMGANGQVVSSNAVGTPDYISPEVLQSQGVDNEYGRECDWWSVGIFLYE
                     MLFGETPFYADSLVGTYGKIMDHKNSLSFPPEVEISEQAKALIRAFLTDRTQRLGRYGI
                     EDIKAHPFFRNDTWSFDNIRESVPPVVPELSSDDDTRNFEDIERDEKPEEVFPVPKGFD
                     GNHLPFIGFTYTGDYQLLSSDTVDAESKEANVANSGAASNNHGHGHNHRH"
     primer_bind     5249..5268
                     /label=rhokforeco_for
     primer_bind     5329..5342
                     /label=EGFP-for
     primer_bind     5518..5546
                     /label=rohkMrefor
     primer_bind     complement(5518..5546)
                     /label=rhokMrerev
     primer_bind     6127..6138
                     /label=C-CMV-24
     primer_bind     complement(6581..6604)
                     /label=rhokrevxho_rev
     primer_bind     6590..6630
                     /label=cibnforEag
     primer_bind     6592..6613
                     /label=dGBP1_EagI_for
     primer_bind     6605..6630
                     /label=cibnXhofor
     misc_feature    6611..7123
                     /label=CIBN delNLS
     primer_bind     complement(7102..7160)
                     /label=CIBNnohaXbarev
     misc_feature    7124..7150
                     /label=HA tag
     primer_bind     complement(7132..7160)
                     /label=cibnhaXbarev
     misc_feature    7151..7153
                     /label=Stop
                     /label=Stop(1)
     primer_bind     complement(7203..7221)
                     /label=UAS-2
     intron          7237..7302
                     /label=small t intron
                     /note="simian virus 40 (SV40) small t antigen intron"
     primer_bind     complement(7310..7329)
                     /label=reverseUAS
     CDS             7432..7452
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(7630..7649)
                     /label=SV40-pA-rev
     polyA_signal    complement(7663..7854)
                     /label=SV40 late polyA
                     /label=SV40 late polyA(1)
     polyA_signal    complement(7663..7854)
                     /label=SV40 late polyA
     primer_bind     complement(7674..7691)
                     /label=EGFP-C1-anti
     primer_bind     complement(7684..7705)
                     /label=FastBac-rev
     polyA_signal    7724..7858
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7724..7741)
                     /label=EGFP-C-Rev
     primer_bind     7761..7782
                     /label=pCI-rev
     primer_bind     7815..7834
                     /label=EBV-rev
     primer_bind     complement(7858..7889)
                     /label=SPLNK-BOT
     protein_bind    7887..7956
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    7896..7941
                     /label=attB
     primer_bind     7921..7943
                     /label=CDS reentry seq rev
     primer_bind     complement(8173..8192)
                     /label=T3
     promoter        complement(8175..8193)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8175..8191)
                     /label=T3
     primer_bind     complement(8181..8203)
                     /label=mKate_rev_SpeI
     primer_bind     complement(8210..8230)
                     /label=M13-rev
     primer_bind     complement(8214..8230)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8214..8230)
                     /label=M13r
     primer_bind     complement(8228..8247)
                     /label=LacOp-for
     misc_binding    complement(8236..8258)
                     /label=LacO
     protein_bind    8238..8254
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(8240..8258)
                     /label=pET-T7side
     promoter        complement(join(8262..8268,8269..8286,8287..8292))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(8262..8281)
                     /label=pUCM13-rev-157
     protein_bind    8307..8328
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8598..9280)
                     /direction=LEFT
                     /label=ColE1 origin
     rep_origin      complement(8616..9204)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    8672..8677
                     /label=Kozak
     primer_bind     8732..8743
                     /label=0 for
     misc_feature    9029..9034
                     /label=Kozak
     primer_bind     9073..9084
                     /label=0 for
     primer_bind     9121..9140
                     /label=PPZ out near XbaI
     CDS             complement(join(9375..10166,10167..10235))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(9378..10037)
                     /label=AmpR
     primer_bind     complement(9425..9444)
                     /label=AmpStop
     primer_bind     9960..9971
                     /label=5'SPLNK-PB#2
     primer_bind     10171..10190
                     /label=AmpStart
     promoter        complement(10236..10340)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    join(10359..10363,1)
                     /label=Kozak
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagcat aacttcgtat aatgtatgct atacgaagtt atgctagcgg atccaagctt
     4861 gcatgcctgc aggtcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
     4921 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagactcta
     4981 gcgagcgccg gagtataaat agaggcgctt cgtctacgga gcgacaattc aattcaaaca
     5041 agcaaagtga acacgtcgct aagcgaaagc taagcaaata aacaagcgca gctgaacaag
     5101 ctaaacaatc tgcagtaaag tgcaagttaa agtgaatcaa ttaaaagtaa ccagcaacca
     5161 agtaaatcaa ctgcaactac tgaaatctgc caagaagtaa ttattgaata caagaagaga
     5221 actctgaata gggaattggg aattcaaaat gccagctgga cgagaaactg tgaccaagca
     5281 gcgcagcatg gatgtggaac gaaggcgccg ggcgaatacg ctcgagcgcg agatgcgcga
     5341 tccgaccagc atctgcaatg tggactgcct gctggacacg gtgtcggcct tggtcagcga
     5401 ttgtgaccac gagtccctga ggcggcttaa gaacatcgag cagtatgcgg ccaaatacaa
     5461 accattggca atgcagatca atcagctgcg catgaacgtc gaggatttcc attttatcaa
     5521 actcatcggc gccggcgcct ttggagaggt gcagctggtg cggcacaagt cctccagcca
     5581 ggtgtacgcc atgaagcggc tgtccaaatt cgagatgatg aagcgaccgg actccgcgtt
     5641 cttctgggag gagcgacaca ttatggcgca cgccaactcc gaatggatcg ttcagctgca
     5701 ctttgcattt caggatgcca aatacctata catggtgatg gactttatgc ccggcggcga
     5761 tatagtctcg ctgatgggtg actacgacat cccggagaag tgggccatct tttacacaat
     5821 ggaggtggtt ctcgcgctgg acaccattca caatatgggc ttcgtgcatc gggacgttaa
     5881 gccggataat atgctcctcg acagctatgg ccacctgaaa ttggccgact ttggcacctg
     5941 tatgcgaatg ggcgccaatg gccaagtggt gtccagcaat gccgttggca cgccggacta
     6001 catcagcccg gaggtgctgc agtcgcaggg cgtggacaac gagtacgggc gggaatgcga
     6061 ctggtggtcg gtgggcattt tcctctacga gatgctgttc ggcgagacac cattctacgc
     6121 agactcgctg gtgggcacct acggcaagat catggaccac aagaactcgc ttagctttcc
     6181 acccgaggtt gagatcagcg aacaggcgaa ggcactgatc cgcgcctttc tcacggaccg
     6241 aacgcaacgt ttgggtcgct acggcatcga ggacattaag gcgcatccgt ttttccgaaa
     6301 tgatacctgg tcgttcgaca acattcgcga gagtgtgccg cctgtggtgc ccgaactctc
     6361 ctccgacgat gatacgcgaa atttcgagga cattgagcgc gatgagaagc cggaggaagt
     6421 gtttcccgta cccaagggct tcgatggcaa tcacttgccc ttcatcggct ttacgtatac
     6481 gggtgactat cagctgctgt ctagcgacac cgtggatgcg gagtcgaagg aggccaatgt
     6541 ggcaaacagc ggggcggcca gcaataatca cggccacggt cataatcacc gccatcggcc
     6601 gtcgctcgag atgaatggag ctataggagg tgaccttttg ctcaattttc ctgacatgtc
     6661 ggtcctagag cgccaaaggg ctcacctcaa gtacctcaat cccacctttg attctcctct
     6721 cgccggcttc tttgccgatt cttcaatgat taccggcggc gagatggaca gctatctttc
     6781 gactgccggt ttgaatcttc cgatgatgta cggtgagacg acggtggaag gtgattcaag
     6841 actctcaatt tcgccggaaa cgacgcttgg gactggaaat ttcaaggcag cgaagtttga
     6901 tacagagact aaggattgta atgaggcggc gaagaagatg acgatgaaca gagatgacct
     6961 agtagaagaa ggagaagaag agaagtcgaa aataacagag caaaacaatg ggagcacaaa
     7021 aagcatcaag aagatgaaac acaaagccaa gaaagaagag aacaatttct ctaatgattc
     7081 atctaaagtg acgaaggaat tggagaaaac ggattatatt cattacccct acgacgtgcc
     7141 agactacgcg taatctagag gatctttgtg aaggaacctt acttctgtgg tgtgacataa
     7201 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
     7261 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
     7321 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa
     7381 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag
     7441 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat
     7501 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct
     7561 gcactgctat acaagaaaat tatggaaaaa tatttgatgt atagtgcctt gactagagat
     7621 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     7681 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     7741 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7801 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcca
     7861 ctagtgtcga cgatgtaggt cacggtctcg aagccgcggt gcgggtgcca gggcgtgccc
     7921 ttgggctccc cgggcgcgta ctccacctca cccatctggt ccatcatgat gaacgggtcg
     7981 aggtggcggt agttgatccc ggcgaacgcg cggcgcaccg ggaagccctc gccctcgaaa
     8041 ccgctgggcg cggtggtcac ggtgagcacg ggacgtgcga cggcgtcggc gggtgcggat
     8101 acgcggggca gcgtcagcgg gttctcgacg gtcacggcgg gcatgtcgac actagttcta
     8161 gccagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag
     8221 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     8281 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8341 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8401 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8461 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8521 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8581 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8641 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8701 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8761 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8821 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8881 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8941 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9001 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     9061 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9241 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9301 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9361 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9421 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9481 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9541 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9601 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9661 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9721 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9781 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9841 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9901 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9961 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10021 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10081 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10141 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10201 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10261 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    10321 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac
//
