LOCUS       pUC-GW-Kan-VPC          6024 bp    DNA     circular     25-JUL-2025
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-16074224|
COMMENT     VNTDBDATE|-16074229|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pUC-GW-Kan-VPC|
COMMENT     VNTAUTHORNAME|Tom Kuhlman|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
FEATURES             Location/Qualifiers
     terminator      3585..3813
                     /vntifkey="43"
                     /label=SV40\terminator
     promoter        780..1004
                     /vntifkey="29"
                     /label=CMV\promoter
     enhancer        416..779
                     /vntifkey="9"
                     /label=CMV\enhancer
     CDS             1766..2845
                     /vntifkey="4"
                     /label=MAPK14/p38a
     CDS             1046..1762
                     /vntifkey="4"
                     /label=mVenus
     promoter        1015..1032
                     /vntifkey="30"
                     /label=T7\promoter
     RBS             1033..1039
                     /vntifkey="32"
                     /label=RBS
     CDS             2849..3568
                     /vntifkey="4"
                     /label=mCerulean3
     CDS             complement(5009..5824)
                     /vntifkey="4"
                     /label=KanR
     rep_origin      complement(4235..4854)
                     /vntifkey="33"
                     /label=pUC\Ori
BASE COUNT     1584 a      1568 c      1499 g      1373 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagactgtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggattagtt 
      421 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta 
      481 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt 
      541 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg 
      601 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta 
      661 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga 
      721 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg 
      781 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc 
      841 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact 
      901 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt 
      961 gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag cgcttaatac 
     1021 gactcactat agaggaggtt gtacaatggt gagcaagggc gaggagctgt tcaccggggt 
     1081 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg 
     1141 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg 
     1201 caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt 
     1261 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg 
     1321 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga 
     1381 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa 
     1441 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta 
     1501 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat 
     1561 cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 
     1621 ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga gcaaagaccc 
     1681 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct 
     1741 cggcatggac gagctgtaca agggaatgtc tcaggagagg cccacgttct accggcagga 
     1801 gctgaacaag acaatctggg aggtgcccga gcgttaccag aacctgtctc cagtgggctc 
     1861 tggcgcctat ggctctgtgt gtgctgcttt tgacacaaaa acggggttac gtgtggcagt 
     1921 gaagaagctc tccagaccat ttcagtccat cattcatgcg aaaagaacct acagagaact 
     1981 gcggttactt aaacatatga aacatgaaaa tgtgattggt ctgttggacg tttttacacc 
     2041 tgcaaggtct ctggaggaat tcaatgatgt gtatctggtg acccatctca tgggggcaga 
     2101 tctgaacaac attgtgaaat gtcagaagct tacagatgac catgttcagt tccttatcta 
     2161 ccaaattctc cgaggtctaa agtatataca ttcagctgac ataattcaca gggacctaaa 
     2221 acctagtaat ctagctgtga atgaagactg tgagctgaag attctggatt ttggactggc 
     2281 tcggcacaca gatgatgaaa tgacaggcta cgtggccact aggtggtaca gggctcctga 
     2341 gatcatgctg aactggatgc attacaacca gacagttgat atttggtcag tgggatgcat 
     2401 aatggccgag ctgttgactg gaagaacatt gtttcctggt acagaccata ttaaccagct 
     2461 tcagcagatt atgcgtctga caggaacacc ccccgcttat ctcattaaca ggatgccaag 
     2521 ccatgaggca agaaactata ttcagtcttt gactcagatg ccgaagatga actttgcgaa 
     2581 tgtatttatt ggtgccaatc ccctggctgt cgacttgctg gagaagatgc ttgtattgga 
     2641 ctcagataag agaattacag cggcccaagc ccttgcacat gcctactttg ctcagtacca 
     2701 cgatcctgat gatgaaccag tggccgatcc ttatgatcag tcctttgaaa gcagggacct 
     2761 ccttatagat gagtggaaaa gcctgaccta tgatgaagtc atcagctttg tgccaccacc 
     2821 ccttgaccaa gaagagatgg agtccggaat ggtgagcaag ggcgaggagc tgttcaccgg 
     2881 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc 
     2941 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac 
     3001 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgagctggg gcgtgcagtg 
     3061 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga 
     3121 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc 
     3181 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt 
     3241 caaggaggac ggcaacatcc tggggcacaa gctggagtac aacgccatcc acggcaacgt 
     3301 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcggcc tcaactgcaa 
     3361 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga 
     3421 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga 
     3481 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac 
     3541 tctcggcatg gacgagctat acaagtaatt ataatctaga taactgatca taatcagcca 
     3601 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct 
     3661 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagcta ataatggtta 
     3721 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 
     3781 ttgtggtttg tccaaactca tcaatgtatc ttaatcccaa tggcgcgccg agcttggctc 
     3841 gagcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 
     3901 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 
     3961 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 
     4021 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctgt tccgcttcct 
     4081 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 
     4141 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 
     4201 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 
     4261 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 
     4321 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 
     4381 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 
     4441 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 
     4501 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 
     4561 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 
     4621 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 
     4681 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 
     4741 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 
     4801 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 
     4861 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 
     4921 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 
     4981 gtatatatga gtaaacttgg tctgacagtt agaaaaactc atcgagcatc aaatgaaact 
     5041 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg 
     5101 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga 
     5161 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat 
     5221 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca 
     5281 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat 
     5341 caaccaaacc gttattcatt cgtgattgcg cctgagcgaa acgaaatacg cgatcgctgt 
     5401 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat 
     5461 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccag 
     5521 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg 
     5581 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg 
     5641 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc 
     5701 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat 
     5761 cagcatccat gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc 
     5821 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 
     5881 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 
     5941 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 
     6001 ggcgtatcac gaggcccttt tgtc 
//
