LOCUS       pLentiX1-CAG-mRu        9283 bp DNA     circular SYN 25-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9283)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Jul 25, 2025 from SnapGene 7.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9283
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        42..146
                     /gene="bla"
                     /label=AmpR promoter
     CDS             147..1007
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(365..384)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1178..1766
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1667..1686
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1920..1937
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2054..2075
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2090..2120
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2128..2144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2133..2155
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2152..2168
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2152..2168
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2187..2207
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2189..2207
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2235..2461
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2462..2642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2689..2814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    3307..3540
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4036..4152
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     protein_bind    4203..4227
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4241..4261
                     /label=pENTR-Gibson-REV
     promoter        4271..4272
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     enhancer        4275..4635
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4637..4914
                     /label=chicken beta-actin promoter
     intron          4915..5931
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     regulatory      5984..5993
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5990..6700
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     CDS             6701..6715
                     /codon_start=1
                     /label=GGGGS linker
                     /translation="GGGGS"
     CDS             6716..6730
                     /codon_start=1
                     /label=GGGGS linker
                     /translation="GGGGS"
     CDS             6731..7297
                     /codon_start=1
                     /locus_tag="hRAS"
                     /label=hRAS
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     misc_feature    7311..7899
                     /label=WPRE
                     /note="woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
     protein_bind    complement(7913..7937)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7939
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             8036..8269
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8341..8462
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8378..8397)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8432..8451
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8502..8637
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8564..8583
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(8657..8676)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8658..8676)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8686..8703)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21)
                     Forward"
     primer_bind     complement(8686..8702)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8695..8717)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8844..9283,1..16)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8931..8950)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9141..9162
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
       61 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      121 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      181 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      241 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      301 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      361 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      421 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
      481 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
      541 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
      601 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
      661 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
      721 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
      781 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
      841 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
      901 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
      961 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1021 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1081 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1141 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1201 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1261 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1321 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1381 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1441 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     1501 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     1561 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1621 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1681 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1741 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     1801 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     1861 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     1921 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     1981 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     2041 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     2101 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     2161 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     2221 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     2281 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     2341 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     2401 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     2461 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     2521 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     2581 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     2641 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     2701 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     2761 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     2821 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     2881 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     2941 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     3001 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     3061 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     3121 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     3181 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     3241 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     3301 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     3361 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     3421 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     3481 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     3541 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     3601 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     3661 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     3721 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     3781 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     3841 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     3901 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     3961 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
     4021 acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     4081 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     4141 attcaaaatt ttatcgtcca gtgtggtggc tactcatagc aggtgccact agtggagttc
     4201 cgacaagttt gtacaaaaaa gcaggctcca ccatgggaac caattcagtc gactggatcc
     4261 ggtactacta ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     4321 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     4381 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     4441 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     4501 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     4561 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     4621 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     4681 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     4741 gcgggggggg ggggggggcg cgcgccrggs ggggsggggs ggggsgrggg gsggggsggg
     4801 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     4861 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     4921 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
     4981 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
     5041 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt
     5101 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt
     5161 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg
     5221 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt
     5281 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
     5341 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct
     5401 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg
     5461 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
     5521 cctcgggccg gggrgggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt
     5581 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
     5641 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
     5701 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     5761 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
     5821 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
     5881 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
     5941 aacgtgctgg ttattgtgct gtctcatcat tttggcaaaa atggccacca tggtgtctaa
     6001 gggcgaagag ctgatcaagg aaaatatgcg tatgaaggtg gtcatggaag gttcggtcaa
     6061 cggccaccaa ttcaaatgca caggtgaagg agaaggcaat ccgtacatgg gaactcaaac
     6121 catgaggatc aaagtcatcg agggaggacc cctgccattt gcctttgaca ttcttgccac
     6181 gtcgttcatg tatggcagcc gtacttttat caagtacccg aaaggcattc ctgatttctt
     6241 taaacagtcc tttcctgagg gttttacttg ggaaagagtt acgagatacg aagatggtgg
     6301 agtcgtcacc gtcatgcagg acaccagcct tgaggatggc tgtctcgttt accacgtcca
     6361 agtcagaggg gtaaactttc cctccaatgg tcccgtgatg cagaagaaga ccaagggttg
     6421 ggagcctaat acagagatga tgtatccagc agatggtggt ctgaggggat acactcatat
     6481 ggcactgaaa gttgatggtg gtggccatct gtcttgctct ttcgtaacaa cttacaggtc
     6541 aaaaaagacc gtcgggaaca tcaagatgcc cggtatccat gccgttgatc accgcctgga
     6601 aaggttagag gaaagtgaca atgaaatgtt cgtagtacaa cgcgaacacg cagttgccaa
     6661 gttcgccggg cttggtggtg ggatggacga gctgtacaag ggtggtgggg gaagcggagg
     6721 aggaggttcg atgaccgaat acaagctggt ggtggtgggc gccgtcggtg tgggcaagag
     6781 tgcgctgacc atccagctga tccagaacca ttttgtggac gaatacgacc ccactataga
     6841 ggattcctac cggaagcagg tggtcattga tggggagacg tgcctgttgg acatcctgga
     6901 taccgccggc caggaggagt acagcgccat gcgggaccag tacatgcgca ccggggaggg
     6961 cttcctgtgt gtgtttgcca tcaacaacac caagtctttt gaggacatcc accagtacag
     7021 ggagcagatc aaacgggtga aggactcgga tgacgtgccc atggtgctgg tggggaacaa
     7081 gtgtgacctg gctgcacgca ctgtggaatc tcggcaggct caggacctcg cccgaagcta
     7141 cggcatcccc tacatcgaga cctcggccaa gacccggcag ggagtggagg atgccttcta
     7201 cacgttggtg cgtgagatcc ggcagcacaa gctgcggaag ctgaaccctc ctgatgagag
     7261 tggccccggc tgcatgagct gcaagtgtgt gctctcctaa caacgccgat aatcaacctc
     7321 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     7381 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     7441 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     7501 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     7561 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     7621 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     7681 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     7741 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     7801 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     7861 ctcagacgag tcggatctcc ctttgggccg cctccccgcc gctgatatct agacccagct
     7921 ttcttgtaca aagtggtcac ctgctatgcg ctgatatagg gcccggtacc tttaagacca
     7981 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     8041 gggctagctc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     8101 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8161 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8221 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     8281 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     8341 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8401 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8461 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     8521 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     8581 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     8641 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     8701 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     8761 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     8821 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     8881 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     8941 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     9001 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     9061 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     9121 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     9181 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     9241 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa ata
//
