LOCUS       .                       6630 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    3815..3848
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     intron          3771..3811
                     /label="Splicing Donor"
                     /note="color: noColor"
     promoter        4845..4949
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   3893..4033
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     rep_origin      4108..4563
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      5981..6569
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             4950..5810
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /product="β-lactamase"
                     /gene="bla"
                     /transl_table=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /codon_start=1
                     /label="AmpR"
     CDS             724..3770
                     /translation="MKPRAECCSPKFWLVLAVLAVSGSRARSQKSPPSIGIAVILVGTS
                     DEVAIKDAHEKDDFHHLSVVPRVELVAMNETDPKSIITRICDLMSDRKIQGVVFADDTD
                     QEAIAQILDFISAQTLTPILGIHGGSSMIMADKDESSMFFQFGPSIEQQASVMLNIMEE
                     YDWYIFSIVTTYFPGYQDFVNKIRSTIENSFVGWELEEVLLLDMSLDDGDSKIQNQLKK
                     LQSPIILLYCTKEEATYIFEVANSVGLTGYGYTWIVPSLVAGDTDTVPAEFPTGLISVS
                     YDEWDYGLPARVRDGIAIITTAASDMLSEHSFIPEPKSSCYNTHEKRIYQSNMLNRYLI
                     NVTFEGRNLSFSEDGYQMHPKLVIILLNKERKWERVGKWKDKSLQMKYYVWPRMCPETE
                     EQEDDHLSIVTLEEAPFVIVESVDPLSGTCMRNTVPCQKRIVTENKTDEEPGYIKKCCK
                     GFCIDILKKISKSVKFTYDLYLVTNGKHGKKINGTWNGMIGEVVMKRAYMAVGSLTINE
                     ERSEVVDFSVPFIETGISVMVSRSNGTVSPSAFLEPFSADVWVMMFVMLLIVSAVAVFV
                     FEYFSPVGYNRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVW
                     AFFAVIFLASYTANLAAFMIQEEYVDQVSGLSDKKFQRPNDFSPPFRFGTVPNGSTERN
                     IRNNYAEMHAYMGKFNQRGVDDALLSLKTGKLDAFIYDAAVLNYMAGRDEGCKLVTIGS
                     GKVFASTGYGIAIQKDSGWKRQVDLAILQLFGDGEMEELEALWLTGICHNEKNEVMSSQ
                     LDIDNMAGVFYMLGAAMALSLITFICEHLFYWQFRHCFMGVCSGKPGMVFSISRGIYSC
                     IHGVAIEERQSVMNSPTATMNNTHSNILRLLRTAKNMANLSGVNGSPQSALDFIRRESS
                     VYDISEHRRSFTHSDCKSYNNPPCEENLFSDYISEVERTFGNLQLKDSNVYQDHYHHHH
                     RPHSIGSASSIDGLYDCDNPPFTTQS"
                     /product="glutamate receptor ionotropic, NMDA 2B isoform a
                     precursor"
                     /gene="GRIN2B"
                     /transl_table=1
                     /note="isoform a precursor is encoded by transcript variant
                     1; glutamate [NMDA] receptor subunit epsilon-2; glutamate
                     receptor subunit epsilon-2; N-methyl-D-aspartate receptor
                     subunit 3; N-methyl D-aspartate receptor subtype 2B;
                     GluN2B(alt_5'UTR); glutamate receptor ionotropic, NMDA 2B;
                     glutamate receptor, ionotropic, N-methyl D-aspartate 2B"
                     /note="color: #993366"
                     /protein_id="NP_000825.2"
                     /db_xref="{'GI': '167003331', 'CCDS': 'CCDS8662.1',
                     'GeneID': '2904', 'HGNC': 'HGNC:4586', 'MIM': '138252'}"
                     /gene_synonym="DEE27; EIEE27; GluN2B; hNR3; MRD6; NMDAR2B;
                     NR2B; NR3"
                     /codon_start=1
                     /label="GRIN2B"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgaagc ccagagcgga gtgctgttct cccaagttct ggttggtgtt ggccgtcctg
      781 gccgtgtcag gcagcagagc tcgttctcag aagagccccc ccagcattgg cattgctgtc
      841 atcctcgtgg gcacttccga cgaggtggcc atcaaggatg cccacgagaa agatgatttc
      901 caccatctct ccgtggtacc ccgggtggaa ctggtagcca tgaatgagac cgacccaaag
      961 agcatcatca cccgcatctg tgatctcatg tctgaccgga agatccaggg ggtggtgttt
     1021 gctgatgaca cagaccagga agccatcgcc cagatcctcg atttcatttc agcacagact
     1081 ctcaccccca tcctgggcat ccacgggggc tcctctatga taatggcaga taaggatgaa
     1141 tcctccatgt tcttccagtt tggcccatca attgaacagc aagcttccgt aatgctcaac
     1201 atcatggaag aatatgactg gtacatcttt tctatcgtca ccacctattt ccctggctac
     1261 caggactttg taaacaagat ccgcagcacc attgagaata gctttgtggg ctgggagcta
     1321 gaggaggtcc tcctactgga catgtccctg gacgatggag attctaagat ccagaatcag
     1381 ctcaagaaac ttcaaagccc catcattctt ctttactgta ccaaggaaga agccacctac
     1441 atctttgaag tggccaactc agtagggctg actggctatg gctacacgtg gatcgtgccc
     1501 agtctggtgg caggggatac agacacagtg cctgcggagt tccccactgg gctcatctct
     1561 gtatcatatg atgaatggga ctatggcctc cccgccagag tgagagatgg aattgccata
     1621 atcaccactg ctgcttctga catgctgtct gagcacagct tcatccctga gcccaaaagc
     1681 agttgttaca acacccacga gaagagaatc taccagtcca atatgctaaa taggtatctg
     1741 atcaatgtca cttttgaggg gaggaatttg tccttcagtg aagatggcta ccagatgcac
     1801 ccgaaactgg tgataattct tctgaacaag gagaggaagt gggaaagggt ggggaagtgg
     1861 aaagacaagt ccctgcagat gaagtactat gtgtggcccc gaatgtgtcc agagactgaa
     1921 gagcaggagg atgaccatct gagcattgtg accctggagg aggcaccatt tgtcattgtg
     1981 gaaagtgtgg accctctgag tggaacctgc atgaggaaca cagtcccctg ccaaaaacgc
     2041 atagtcactg agaataaaac agacgaggag ccgggttaca tcaaaaaatg ctgcaagggg
     2101 ttctgtattg acatccttaa gaaaatttct aaatctgtga agttcaccta tgacctttac
     2161 ctggttacca atggcaagca tgggaagaaa atcaatggaa cctggaatgg tatgattgga
     2221 gaggtggtca tgaagagggc ctacatggca gtgggctcac tcaccatcaa tgaggaacga
     2281 tcggaggtgg tcgacttctc tgtgcccttc atagagacag gcatcagtgt catggtgtca
     2341 cgcagcaatg ggactgtctc accttctgcc ttcttagagc cattcagcgc tgacgtatgg
     2401 gtgatgatgt ttgtgatgct gctcatcgtc tcagccgtgg ctgtctttgt ctttgagtac
     2461 ttcagccctg tgggttataa caggtgcctc gctgatggca gagagcctgg tggaccctct
     2521 ttcaccatcg gcaaagctat ttggttgctc tggggtctgg tgtttaacaa ctccgtacct
     2581 gtgcagaacc caaaggggac cacctccaag atcatggtgt cagtgtgggc cttctttgct
     2641 gtcatcttcc tggccagcta cactgccaac ttagctgcct tcatgatcca agaggaatat
     2701 gtggaccagg tttctggcct gagcgacaaa aagttccaga gacctaatga cttctcaccc
     2761 cctttccgct ttgggaccgt gcccaacggc agcacagaga gaaatattcg caataactat
     2821 gcagaaatgc atgcctacat gggaaagttc aaccagaggg gtgtagatga tgcattgctc
     2881 tccctgaaaa cagggaaact ggatgccttc atctatgatg cagcagtgct gaactatatg
     2941 gcaggcagag atgaaggctg caagctggtg accattggca gtgggaaggt ctttgcttcc
     3001 actggctatg gcattgccat ccaaaaagat tctgggtgga agcgccaggt ggaccttgct
     3061 atcctgcagc tctttggaga tggggagatg gaagaactgg aagctctctg gctcactggc
     3121 atttgtcaca atgagaagaa tgaggtcatg agcagccagc tggacattga caacatggca
     3181 ggggtcttct acatgttggg ggcggccatg gctctcagcc tcatcacctt catctgcgaa
     3241 caccttttct attggcagtt ccgacattgc tttatgggtg tctgttctgg caagcctggc
     3301 atggtcttct ccatcagcag aggtatctac agctgcatcc atggggtggc gatcgaggag
     3361 cgccagtctg taatgaactc ccccaccgca accatgaaca acacacactc caacatcctg
     3421 cgcctgctgc gcacggccaa gaacatggct aacctgtctg gtgtgaatgg ctcaccgcag
     3481 agcgccctgg acttcatccg acgggagtca tccgtctatg acatctcaga gcaccgccgc
     3541 agcttcacgc attctgactg caaatcctac aacaacccgc cctgtgagga gaacctcttc
     3601 agtgactaca tcagtgaggt agagagaacg ttcgggaacc tgcagctgaa ggacagcaac
     3661 gtgtaccaag atcactacca ccatcaccac cggccccata gtattggcag tgccagctcc
     3721 atcgatgggc tctacgactg tgacaaccca cccttcacca cccagtccag gtaagtatca
     3781 aggttacaag acaggtttaa ggagaccaat aagctattat tcgtatagga tactttatac
     3841 gaagttatta agttatggag accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc
     3901 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     3961 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     4021 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     4081 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     4141 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4201 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4261 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4321 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4381 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     4441 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     4501 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     4561 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     4621 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     4681 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     4741 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     4801 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4861 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4921 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4981 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5041 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5101 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5161 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5221 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5281 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5341 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5401 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5461 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5521 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5581 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5641 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5701 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5761 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5821 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5881 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5941 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6001 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6061 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6121 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6181 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6241 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6301 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6361 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6421 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6481 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6541 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6601 tggccttttg ctggcctttt gctcacatgt
//
