LOCUS       .                       5784 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             727..753
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
                     /transl_table=1
                     /codon_start=1
                     /label="HA"
                     /note="color: #cc99b2"
     misc_feature    2969..3002
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     intron          2925..2965
                     /label="Splicing Donor"
                     /note="color: noColor"
     promoter        3999..4103
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   3047..3187
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     rep_origin      3262..3717
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      5135..5723
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             4104..4964
                     /product="β-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /transl_table=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /codon_start=1
                     /label="AmpR"
     CDS             760..2924
                     /product="IQ motif and SEC7 domain-containing protein 2
                     isoform 1"
                     /translation="MEAGSGPPGGPGSESPNRAVEYLLELNNIIESQQQLLETQRRRIE
                     ELEGQLDQLTQENRDLREESQLHRGELHRDPHGARDSPGRESQYQNLRETQFHHRELRE
                     SQFHQAARDVGYPNREGAYQNREAVYRDKERDASYPLQDTTGYTARERDVAQCHLHHEN
                     PALGRERGGREAGPAHPGREKEAGYSAAVGVGPRPPRERGQLSRGASRSSSPGAGGGHS
                     TSTSTSPATTLQRKSDGENSRTVSVEGDAPGSDLSTAVDSPGSQPPYRLSQLPPSSSHM
                     GGPPAGVGLPWAQRARLQPASVALRKQEEEEIKRSKALSDSYELSTDLQDKKVEMLERK
                     YGGSFLSRRAARTIQTAFRQYRMNKNFERLRSSASESRMSRRIILSNMRMQFSFEEYEK
                     AQNPAYFEGKPASLDEGAMAGARSHRLERGLPYGGSCGGGIDGGGSSVTTSGEFSNDIT
                     ELEDSFSKQVKSLAESIDEALNCHPSGPMSEEPGSAQLEKRESKEQQEDSSATSFSDLP
                     LYLDDTVPQQSPERLPSTEPPPQGRPEFWAPAPLPPVPPPVPSGTREDGSREEGTRRGP
                     GCLECRDFRLRAAHLPLLTIEPPSDSSVDLSDRSDRGSVHRQLVYEADGCSPHGTLKHK
                     GPPGRAPIPHRHYPAPEGPAPAPPGPLPPAPNSGTGPSGVAGGRRLGKCEAAGENSDGG
                     DNESLESSSNSNETINCSSGSSSRDSL"
                     /gene="IQSEC2"
                     /transl_table=1
                     /note="isoform 1 is encoded by transcript variant 1; IQ
                     motif and SEC7 domain-containing protein 2; brefeldin A
                     resistant Arf-guanine nucleotide exchange factor 1b;
                     brefeldin A resistant Arf-guanine nucleotide exchange
                     factor 1c; IQ motif and Sec7 domain 2"
                     /note="color: #993366"
                     /protein_id="NP_001104595.1"
                     /codon_start=1
                     /db_xref="{'GI': '162138911', 'CCDS': 'CCDS48130.1',
                     'GeneID': '23096', 'HGNC': 'HGNC:29059', 'MIM': '300522'}"
                     /gene_synonym="BRAG1; IQ-ArfGEF; MRX1; MRX18; MRX78; XLID1"
                     /label="IQSEC2"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatcta tggaggcggg gtcggggccc
      781 ccgggcggcc cgggatccga gagcccaaat cgggccgtgg agtacctgct ggagctgaac
      841 aacatcatcg agagccagca gcagctgctg gaaacccagc ggcggcgcat cgaggagctg
      901 gagggccagc tggaccagct cacccaggag aaccgcgacc tgcgagagga gagccagctg
      961 caccgcgggg agctgcaccg ggacccccac ggcgcgcggg atagcccggg ccgcgagagc
     1021 cagtaccaga acctgcgcga gacccagttc caccaccgcg agctgcggga gagccagttc
     1081 caccaggcgg cccgggacgt gggctacccg aaccgggaag gcgcctacca gaatcgggag
     1141 gctgtgtatc gggacaagga gcgggacgcc tcctacccgc tccaggacac taccggttac
     1201 acagcccgcg agcgtgacgt ggcccagtgc cacctgcacc acgagaaccc agccctgggt
     1261 cgcgagcgtg gcgggcggga ggccgggccg gcgcacccgg gccgcgagaa ggaagcgggc
     1321 tattcggcgg cggtgggcgt ggggccgcgg ccaccgcggg agcggggcca gctgagccgt
     1381 ggcgcatcca ggagctccag tcccggcgcc ggcggaggcc acagcaccag taccagcacc
     1441 agcccggcca cgaccctcca gagaaaatct gatggtgaga attccagaac agtcagtgtg
     1501 gagggtgatg ccccaggcag tgacctgagc acagcggttg atagtcctgg gagccaaccc
     1561 ccctaccggc tgagccagct gcccccctcc agcagccaca tggggggccc ccctgctgga
     1621 gtgggccttc cctgggctca gcgggcacgc ctccagccag ccagtgtcgc cctgaggaag
     1681 caggaggagg aggagataaa gcgctccaag gccctatcgg acagctatga actctccaca
     1741 gacctgcagg acaagaaggt ggaaatgctg gagaggaagt atgggggctc cttcctgagc
     1801 cgcagggctg ccaggaccat ccagacagcc ttccgccagt accgcatgaa caagaacttt
     1861 gagcggctac gcagctcagc ctcagagagc cgcatgtccc gccgcatcat cctttccaac
     1921 atgcggatgc agttctcctt tgaggagtat gagaaggcac agaaccccgc gtacttcgag
     1981 ggcaagcctg cctcgctgga cgagggtgcc atggctggtg cccggagcca ccggcttgaa
     2041 cgggggctcc catatggagg ctcctgtggt gggggcatcg atggtggtgg aagctccgtc
     2101 accacatctg gagagttttc taatgacatc acagaacttg aggactcctt ctccaaacag
     2161 gtaaagtctc tggctgaatc catcgacgaa gccctgaact gccacccgtc agggcccatg
     2221 tctgaggagc cagggtcagc ccagctggag aagcgggagt caaaggaaca gcaagaggac
     2281 agctcagcca catccttcag tgatcttccc ctctacctgg atgacacagt tccccaacaa
     2341 tcccctgagc gactgcccag cacagaaccc ccaccccagg gccggcccga gttctgggcg
     2401 ccagcccctc tcccgccagt tcctccacca gtgccatcag gaacccggga agacggtagc
     2461 cgtgaggaag gcactcgcag gggtcccggg tgcttggagt gccgggattt ccggctgcgg
     2521 gctgcccacc ttcccctgct taccattgag cctcctagtg acagctccgt ggacctgagt
     2581 gaccgctcag atcgcggctc tgtccaccgc cagctggtgt atgaggctga tggctgcagc
     2641 ccccatggga ccctgaagca caaggggcca ccaggcaggg ccccgatccc acaccgccac
     2701 tacccagccc ctgaaggccc agccccagcc ccaccagggc ccctgccacc agcccccaac
     2761 agtggcactg ggcccagtgg tgtggctggg ggtcggaggt tggggaagtg cgaggcagca
     2821 ggcgagaact ctgatggtgg agataacgag agccttgaga gctccagcaa ttccaatgag
     2881 accatcaact gcagctccgg ctcctcttct cgggacagtc taaggtaagt atcaaggtta
     2941 caagacaggt ttaaggagac caataagcta ttattcgtat aggatacttt atacgaagtt
     3001 attaagttat ggagaccggt gtggcacgtg cggaccgagc ggccgcagga acccctagtg
     3061 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
     3121 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc
     3181 ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     3241 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     3301 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     3361 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     3421 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
     3481 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     3541 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     3601 cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     3661 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
     3721 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     3781 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     3841 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     3901 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     3961 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4021 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     4081 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4141 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     4201 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     4261 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     4321 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     4381 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4441 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4501 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     4561 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     4621 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     4681 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     4741 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     4801 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     4861 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     4921 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     4981 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5041 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5101 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5161 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5221 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5281 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5341 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5401 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5461 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5521 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5581 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5641 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5701 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5761 tttgctggcc ttttgctcac atgt
//
