LOCUS       .                       7078 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1493..3791
                     /translation="EPPATGLCKQTYQRETRHSWDSPAFNNDVVQRRHYRIGLNLFNKK
                     PEKGIQYLIERGFLSDTPVGVAHFILERKGLSRQMIGEFLGNRQKQFNRDVLDCVVDEM
                     DFSSMDLDDALRKFQSHIRVQGEAQKVERLIEAFSQRYCVCNPALVRQFRNPDTIFILA
                     FAIILLNTDMYSPSVKAERKMKLDDFIKNLRGVDNGEDIPRDLLVGIYQRIQGRELRTN
                     DDHVSQVQAVERMIVGKKPVLSLPHRRLVCCCQLYEVPDPNRPQRLGLHQREVFLFNDL
                     LVVTKIFQKKKILVTYSFRQSFPLVEMHMQLFQNSYYQFGIKLLSAVPGGERKVLIIFN
                     APSLQDRLRFTSDLRESIAEVQEMEKYRVESELEKQKGMMRPNASQPGGAKDSVNGTMA
                     RSSLEDTYGAGDGLKRGALSSSLRDLSDAGKRGRRNSVGSLDSTIEGSVISSPRPHQRM
                     PPPPPPPPPEEYKSQRPVSNSSSFLGSLFGSKRGKGPFQMPPPPTGQASASSSSASSTH
                     HHHHHHHHGHSHGGLGVLPDGQSKLQALHAQYCQGPGPAPPPYLPPQQPSLPPPPQQPP
                     PLPQLGSIPPPPASAPPVGPHRHFHAHGPVPGPQHYTLGRPGRAPRRGAGGHPQFAPHG
                     RHPLHQPTSPLPLYSPAPQHPPAHKQGPKHFIFSHHPQMMPAAGAAGGPGSRPPGGSYS
                     HPHHPQSPLSPHSPIPPHPSYPPLPPPSPHTPHSPLPPTSPHGPLHASGPPGTANPPSA
                     NPKAKPSRISTVV"
                     /product="IQ motif and SEC7 domain-containing protein 2
                     isoform 1"
                     /gene="IQSEC2"
                     /transl_table=1
                     /note="isoform 1 is encoded by transcript variant 1; IQ
                     motif and SEC7 domain-containing protein 2; brefeldin A
                     resistant Arf-guanine nucleotide exchange factor 1b;
                     brefeldin A resistant Arf-guanine nucleotide exchange
                     factor 1c; IQ motif and Sec7 domain 2"
                     /note="color: #993366"
                     /protein_id="NP_001104595.1"
                     /db_xref="{'GI': '162138911', 'CCDS': 'CCDS48130.1',
                     'GeneID': '23096', 'HGNC': 'HGNC:29059', 'MIM': '300522'}"
                     /gene_synonym="BRAG1; IQ-ArfGEF; MRX1; MRX18; MRX78; XLID1"
                     /codon_start=1
                     /label="IQSEC2"
     CDS             5398..6258
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /product="β-lactamase"
                     /gene="bla"
                     /transl_table=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /codon_start=1
                     /label="AmpR"
     rep_origin      6429..7017
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     rep_origin      4556..5011
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     terminator      3867..4298
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   4341..4481
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     promoter        5293..5397
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     CDS             3798..3863
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /codon_start=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     CDS             288..1318
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /product="site-specific recombinase"
                     /transl_table=1
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /protein_id=""
                     /codon_start=1
                     /label="Cre"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     protein_bind    1367..1400
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /label="lox2272-JTZ17"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac agggagcctc
     1501 cggctaccgg cctgtgcaag cagacttacc agcgggagac aaggcatagc tgggactcgc
     1561 cagctttcaa caatgatgtg gtccagaggc ggcactaccg aatcggcctc aacctcttca
     1621 acaagaagcc agagaagggt atccagtatc tgatcgagcg gggcttcctg tcagacacac
     1681 cggtgggagt ggctcacttc atcctggagc ggaaaggcct cagccggcag atgatagggg
     1741 aattcctagg gaaccggcag aagcagttca acagagacgt gttggactgt gtggtggatg
     1801 agatggactt ctcctccatg gatctggatg atgcgctccg gaagttccag tcccatatcc
     1861 gggttcaggg tgaggcccag aaagtggagc gactcatcga agccttcagc cagcggtact
     1921 gtgtctgtaa cccagccctc gtgcgccagt tccggaaccc agacaccatc ttcatccttg
     1981 cttttgccat catcctcctc aataccgaca tgtacagtcc cagcgtcaaa gctgaacgaa
     2041 agatgaaact agatgacttc atcaagaacc tgagaggggt tgacaatggt gaagacatcc
     2101 cccgagacct cctggtgggc atctaccagc gcatccaggg gcgtgaactg cggaccaacg
     2161 atgaccatgt gtcccaggtg caggctgtgg agcgcatgat tgttggaaag aaaccagtcc
     2221 tgtctctccc tcaccgtcga ctggtttgct gctgccagct ctacgaggtg ccagatccaa
     2281 accgccccca gaggctaggg ttgcatcagc gggaggtctt cctcttcaat gatctccttg
     2341 tggtcaccaa aattttccag aagaagaaga tcttggtgac gtacagtttc cgtcagtctt
     2401 tccccctcgt ggaaatgcac atgcagctct tccagaattc atattaccag tttgggatca
     2461 agttgctgtc tgcagtacct ggtggggagc gaaaagtcct catcatcttc aatgccccca
     2521 gcctccagga ccggctgcgc tttacatccg acctgcgcga gtccattgcg gaggtgcagg
     2581 agatggagaa ataccgtgtg gagtcggagc tggagaagca gaaaggtatg atgcggccta
     2641 acgcctcaca gcctggaggg gccaaggact cagtgaatgg gacgatggcc cgcagtagcc
     2701 tggaggacac ttacggggca ggcgatgggc tcaaacgggg cgcactcagc agttccctgc
     2761 gagacctctc tgatgcaggg aagcgggggc ggcgtaacag cgtgggatcg ctggacagca
     2821 ccatcgaagg gtctgttatt agcagtccac gccctcacca gaggatgcca cctccgcccc
     2881 cacccccgcc gccagaggag tacaagagcc agaggcccgt ctccaactcc tcatccttcc
     2941 tgggctccct atttggaagc aagcggggca aggggccctt ccagatgcca ccaccgccaa
     3001 caggccaggc ctctgcctcc tcttcatctg cttcttccac gcaccaccac caccaccacc
     3061 accatcatgg ccatagccac ggtggcctgg gggtgctgcc tgatgggcag tccaagctcc
     3121 aggccctgca tgcccagtat tgccaaggac cgggccctgc cccgccaccc tacctcccac
     3181 cccagcagcc ctctcttccc ccacctcccc agcagccccc acccttgccc cagctgggct
     3241 ccattccacc gcctcccgcc tcagccccac ctgtggggcc acatcgccac ttccacgccc
     3301 atggcccagt cccagggccc caacactata ccttgggccg gccaggcagg gcacccagac
     3361 ggggggctgg aggacaccct cagtttgctc cacatggccg ccaccccctg caccagccca
     3421 catccccact gcccctgtac agtcctgccc cccagcaccc tccagcccac aaacagggcc
     3481 ctaagcactt catcttcagc caccacccac agatgatgcc agcagcaggc gcggctgggg
     3541 gccctggatc ccggccacca gggggctcct actcccaccc ccaccacccc cagtcaccat
     3601 tgtcaccaca ctcacccatc ccaccccacc cctcctatcc acccctcccc ccaccctccc
     3661 ctcacacccc gcactcaccc cttccaccca cctcccccca tggcccgctg cacgcctctg
     3721 ggccccctgg cacagccaac ccccccagtg caaaccccaa ggccaagcca agccggatca
     3781 gcaccgtggt ctgtacagac tacaaagacc atgacggtga ttataaagat catgacatcg
     3841 actacaagga tgacgatgac aagtaactcg agataatcaa cctctggatt acaaaatttg
     3901 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     3961 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     4021 taaatcctgg ttagttcttg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     4081 gacaggggct cggctgttgg gcactgacaa ttccgtggtg tttatttgtg aaatttgtga
     4141 tgctattgct ttatttgtaa ccatctagct ttatttgtga aatttgtgat gctattgctt
     4201 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     4261 tgtttcaggt tcagggggag atgtgggagg ttttttaaag atctggagac cggtgtggca
     4321 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
     4381 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     4441 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
     4501 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     4561 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4621 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4681 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4741 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     4801 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4861 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg
     4921 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     4981 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     5041 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     5101 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     5161 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     5221 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     5281 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     5341 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5401 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5461 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5521 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5581 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5641 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5701 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5761 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     5821 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5881 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5941 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     6001 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     6061 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     6121 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     6181 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     6241 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     6301 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6361 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6421 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6481 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6541 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     6601 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6661 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6721 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6781 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6841 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6901 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6961 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7021 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
