LOCUS       .                       7127 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             727..753
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /codon_start=1
                     /label="HA"
                     /note="color: #cc99b2"
     misc_feature    4312..4345
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     intron          4268..4308
                     /label="Splicing Donor"
                     /note="color: noColor"
     promoter        5342..5446
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   4390..4530
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     rep_origin      4605..5060
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      6478..7066
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             5447..6307
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /gene="bla"
                     /product="β-lactamase"
                     /transl_table=1
                     /codon_start=1
                     /label="AmpR"
     CDS             760..4267
                     /translation="YSEWRSLHLVIQNDQGHTSVLHSYPESVGREVANAVVRPLGQVLG
                     TPSVAGSENLLKTDKEVKWTMEVICYGLTLPLDGETVKYCVDVYTDWIMALVLPKDSIP
                     LPVIKEPNQYVQTILKHLQNLFVPRQEQGSSQIRLCLQVLRAIQKLARESSLMARETWE
                     VLLLFLLQINDILLAPPTVQGGIAENLAEKLIGVLFEVWLLACTRCFPTPPYWKTAKEM
                     VANWRHHPAVVEQWSKVICALTSRLLRFTYGPSFPAFKVPDEDASLIPPEMDNECVAQT
                     WFRFLHMLSNPVDLSNPAIISSTPKFQEQFLNVSGMPQELNQYPCLKHLPQIFFRAMRG
                     ISCLVDAFLGISRPRSDSAPPTPVNRLSMPQSAAVSTTPPHNRRHRAVTVNKATMKTST
                     VSTAHASKVQHQTSSTSPLSSPNQTSSEPRPLPAPRRPKVNSILNLFGSWLFDAAFVHC
                     KLHNGINRDSSMTAITTQASMEFRRKGSQMSTDTMVSNPMFDASEFPDNYEAGRAEACG
                     TLCRIFCSKKTGEEILPAYLSRFYMLLIQGLQINDYVCHPVLASVILNSPPLFCCDLKG
                     IDVVVPYFISALETILPDRELSKFKSYVNPTELRRSSINILLSLLPLPHHFGTVKSEVV
                     LEGKFSNDDSSSYDKPITFLSLKLRLVNILIGALQTETDPNNTQMILGAMLNIVQDSAL
                     LEAIGCQMEMGGGENNLKSHSRTNSGISSASGGSTEPTTPDSERPAQALLRDYALNTDS
                     AAGLLIRSIHLVTQRLNSQWRQDMSISLAALELLSGLAKVKVMVDSGDRKRAISSVCTY
                     IVYQCSRPAPLHSRDLHSMIVAAFQCLCVWLTEHPDMLDEKDCLKEVLEIVELGISGSK
                     SKNNEQEVKYKGDKEPNPASMRVKDAAEATLTCIMQLLGAFPSPSGPASPCSLVNETTL
                     IKYSRLPTINKHSFRYFVLDNSVILAMLEQPLGNEQNDFFPSVTVLVRGMSGRLAWAQQ
                     LCLLPRGAKANQKLFVPEPRPVPKNDVGFKYSVKHRPFPEEVDKIPFVKADLSIPDLHE
                     IVTEELEERHEKLRSGMAQQIAYEIHLEQQSEEELQKRSFPDPVTDCKPPPPAQEFQTA
                     RLFLSHFGFLSLEALKEPANSRLPPHLIALDSTIPGFFDDIGYLDLLPCRPFDTVFIFY
                     MKP"
                     /note="isoform 1 is encoded by transcript variant 1; Ral
                     GTPase activating protein, beta subunit (non-catalytic);
                     Ral GTPase activating protein non-catalytic beta subunit"
                     /note="color: #993366"
                     /db_xref="{'GI': '34787409', 'CCDS': 'CCDS13305.1',
                     'GeneID': '57148', 'HGNC': 'HGNC:29221', 'MIM': '618833'}"
                     /gene="RALGAPB"
                     /product="ral GTPase-activating protein subunit beta
                     isoform 1"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /transl_table=1
                     /codon_start=1
                     /protein_id="NP_065069.1"
                     /label="RALGAPB"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatctt actctgagtg gaggtcactg
      781 catttggtga ttcagaatga tcaaggccat accagtgtgc tgcacagcta tccagagagc
      841 gttggacgag aggtggcaaa tgctgtagtc cgtcctcttg ggcaggtgtt aggtacccct
      901 tcagtggctg gtagtgagaa tttgttaaaa actgacaaag aagtaaaatg gaccatggaa
      961 gtaatttgct atggactgac ccttccattg gatggagaga ctgtaaaata ttgcgttgat
     1021 gtatatacag actggattat ggctttagtg ttgccaaaag attctattcc attgccagtt
     1081 attaaagagc ctaatcaata tgttcaaact atactaaaac acctacagaa tctttttgta
     1141 ccaagacagg aacagggttc cagtcagatt cgactatgct tacaggtcct gagagccatt
     1201 cagaaactgg cccgtgagtc atctctcatg gcccgagaaa cttgggaagt cttactgttg
     1261 tttcttctgc agattaacga catacttctg gccccaccaa ctgttcaagg tggcattgct
     1321 gagaatctag cagagaagtt gattggtgtt ctctttgagg tgtggttact agcttgtact
     1381 cggtgcttcc caacacctcc ttattggaaa acagccaagg agatggtggc taactggagg
     1441 catcacccag cagtggtgga gcagtggagc aaggtcattt gtgcactcac ttccagattg
     1501 ctacgcttta catatggtcc ttcatttcct gcatttaaag ttcccgatga agatgccagt
     1561 ctgatccctc cagaaatgga taatgagtgt gttgcacaga catggtttcg ctttttacac
     1621 atgttaagta atcctgtgga tttgagtaac ccagctatta taagctctac tcccaaattt
     1681 caggaacagt tcttgaatgt gagcggaatg ccgcaagaat tgaatcagta tccctgcctt
     1741 aaacatctgc ctcaaatatt ttttcgtgcc atgcgtggaa tcagctgtct ggtggatgca
     1801 ttcttaggta tttctagacc ccgatcagac agtgctcccc caacacccgt gaatagatta
     1861 agtatgcctc aaagtgctgc tgtcagtacc acccccccac ataaccggag gcaccgggct
     1921 gttactgtga ataaggccac catgaagaca agcacagtta gtactgctca tgcctctaaa
     1981 gttcagcacc agacgtcctc cacctctcct ctgtcaagtc caaatcagac tagttcagaa
     2041 ccccggccac tgcctgcccc tcggagacca aaggttaaca gcatcttgaa tctctttgga
     2101 tcatggttat ttgatgcagc atttgttcac tgtaaacttc ataatgggat aaacagagac
     2161 agcagcatga ctgccattac aacacaagct agcatggagt ttcgacggaa agggtcacaa
     2221 atgtccacag acaccatggt ttccaatcct atgtttgatg caagtgaatt tcctgataac
     2281 tatgaagcag gaagagctga ggcttgtggg acactgtgta ggattttttg tagcaagaag
     2341 actggagaag agattctgcc agcttattta tccagatttt acatgctttt aattcaaggt
     2401 ttgcagataa atgattatgt gtgccatcct gtcttggcca gcgttattct aaactctcct
     2461 cctttgttct gctgtgactt gaaagggatt gatgttgtgg ttccttactt tatttcagct
     2521 cttgaaacca ttttgcctga cagagaactc tcaaaattca aaagctatgt aaatccaaca
     2581 gaattgcgaa gatcctccat taatatcctg ctttctttgt tgcccctccc tcatcatttt
     2641 ggcacagtca aatctgaggt ggtcctggaa ggaaagttta gtaacgatga cagctcttct
     2701 tatgataaac caataacttt tctgtccctg aagttgagac ttgtgaatat attaataggt
     2761 gccttgcaaa ctgaaacgga ccccaacaac acccaaatga tattaggggc aatgttaaat
     2821 attgttcaag attcagcact tttggaagcc attggttgcc agatggagat gggtggtgga
     2881 gaaaataacc tgaagagtca tagtcgcacc aatagtggta ttagttcagc aagtggtgga
     2941 agcacggagc ccacgactcc cgatagtgag agacctgctc aagctctctt aagagattat
     3001 gctcttaata cagattcagc tgctgggctc ctgattcgca gcattcatct cgtcacccaa
     3061 agactcaact cccagtggcg ccaagacatg agcatatcac tggcagctct agagctcctc
     3121 tctggccttg caaaggtaaa agtgatggtt gactcaggag accggaagcg agccatcagt
     3181 tctgtgtgca cctacattgt ttatcagtgt agtcggccag ctcctttaca ctccagggat
     3241 ctgcactcca tgatagtggc agcttttcag tgtctctgtg tctggctgac agagcaccct
     3301 gatatgcttg atgaaaagga ctgccttaag gaagtactgg agattgtgga actgggtatc
     3361 tcaggaagta agtccaagaa caatgagcaa gaggtcaagt acaaaggaga taaggagcca
     3421 aaccctgcat ctatgagggt aaaggatgct gctgaagcca ccctaacatg cattatgcag
     3481 ttgctcggcg catttccttc acctagtggt cctgcctctc cttgtagtct tgtgaatgag
     3541 accactttga ttaaatactc caggctgcca accataaaca agcatagttt ccggtacttt
     3601 gtcttggata acagtgtcat cctggcaatg ctggaacaac ctcttggaaa tgagcagaat
     3661 gattttttcc cctctgtcac tgtgctggtc cggggaatgt ctggaagact tgcttgggca
     3721 caacagcttt gtcttttacc cagaggagca aaagcaaatc agaagctttt tgtacctgaa
     3781 cctcgcccag ttcctaaaaa tgacgttgga tttaaatatt ctgtgaaaca tcggccattt
     3841 cctgaagagg tggacaagat tccttttgtg aaagcagatc tcagcattcc agatttgcat
     3901 gaaatagtca ctgaagaatt agaagagaga cacgaaaaat taaggagtgg catggcccag
     3961 cagattgctt atgaaataca ccttgagcaa cagagtgagg aggaattgca gaagagaagt
     4021 tttcctgacc cagttacgga ttgcaagccc ccgcctcctg cccaggaatt ccaaacagcc
     4081 cgcctttttc tctcacactt tggatttttg tccttagaag cactgaagga acctgcaaat
     4141 agtcgtctac ctcctcacct tattgcactt gattccacga tacctggatt ttttgatgac
     4201 attgggtatc tggatctctt gccatgtcgt ccttttgaca cagtttttat tttctatatg
     4261 aagccaggta agtatcaagg ttacaagaca ggtttaagga gaccaataag ctattattcg
     4321 tataggatac tttatacgaa gttattaagt tatggagacc ggtgtggcac gtgcggaccg
     4381 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
     4441 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
     4501 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
     4561 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
     4621 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4681 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4741 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4801 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4861 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4921 tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     4981 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     5041 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
     5101 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     5161 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     5221 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     5281 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     5341 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5401 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5461 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5521 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5581 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5641 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5701 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5761 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5821 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5881 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5941 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6001 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6061 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6121 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6181 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6241 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6301 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6361 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6421 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6481 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6541 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6601 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     6661 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6721 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6781 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6841 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6901 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6961 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7021 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7081 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
