LOCUS       .                       7276 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             760..4416
                     /gene="MBD5"
                     /product="methyl-CpG-binding domain protein 5 isoform 1"
                     /translation="NGGKECDGGDKEGGLPAIQVPVGWQRRVDQNGVLYVSPSGSLLSC
                     LEQVKTYLLTDGTCKCGLECPLILPKVFNFDPGAAVKQRTAEDVKADEDVTKLCIHKRK
                     IIAVATLHKSMEAPHPSLVLTSPGGGTNATPVVPSRAATPRSVRNKSHEGITNSVMPEC
                     KNPFKLMIGSSNAMGRLYVQELPGSQQQELHPVYPRQRLGSSEHGQKSPFRGSHGGLPS
                     PASSGSQIYGDGSISPRTDPLGSPDVFTRSNPGFHGAPNSSPIHLNRTPLSPPSVMLHG
                     SPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSTNMEIPRAMFHHKPPQGPPPPPP
                     PSCALQKKPLTSEKDPLGILDPIPSKPVNQNPVIINPTSFHSNVHSQVPMMNVSMPPAV
                     VPLPSNLPLPTVKPGHMNHGSHVQRVQHSASTSLSPSPVTSPVHMMGTGIGRIEASPQR
                     SRSSSTSSDHGNFMMPPVGPQATSSGIKVPPRSPRSTIGSPRPSMPSSPSTKSDGHHQY
                     KDIPNPLIAGISNVLNTPSSAAFPTASAGSSSVKSQPGLLGMPLNQILNQHNAASFPAS
                     SLLSAAAKAQLANQNKLAGNNSSSSSNSGAVAGSGNTEGHSTLNTMFPPTANMLLPTGE
                     GQSGRAALRDKLMSQQKDALRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGS
                     FPISSMSQLLQSMSCQSSHLSSNSTPGCGASNTALPCSANQLHFTDPSMNSSVLQNIPL
                     RGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQ
                     SRISTSSTPVIPNSIVSSYNQTSSEAGGSGPSSSIAIAGTNHPAITKTTSVLQDGVIVT
                     TAAGNPLQSQLPIGSDFPFVGQEHALHFPSNSTSNNHLPHPLNPSLLSSLPISLPVNQQ
                     HLLNQNLLNILQPSAGEGKSEINLHPLGFLNPNVNAALAFLSSDMDGQVLQPVHFQLLA
                     ALLQNQAQAAAMLPLPSFNLTISDLLQQQNTPLPSLTQMTAPPDHLPSNQSDNSRAETL
                     LTSPLGNPLPSFAGSDTTFNPLFLPAVNGASGLMTLNPQLLGGVLNSASANTANHPEVS
                     IATSSQATTTTTTTSSAVAALTVSTLGGTAVVSMAETLLNISNNAGNTPGPAKLNSNSV
                     VPQLLNPLLGTGLLGDMSSINNTLSNHQLTHLQSLLNNNQMFPPNQQQQQLLQ"
                     /note="isoform 1 is encoded by transcript variant 1;
                     methyl-CpG-binding protein MBD5"
                     /note="color: #993366"
                     /db_xref="{'GI': '1802776415', 'CCDS': 'CCDS92873.1',
                     'GeneID': '55777', 'HGNC': 'HGNC:20444', 'MIM': '611472'}"
                     /gene_synonym="C2DELq23.1; DEL2Q23.1; MRD1"
                     /codon_start=1
                     /transl_table=1
                     /protein_id="NP_001365049.1"
                     /label="MBD5"
     CDS             5596..6456
                     /gene="bla"
                     /product="β-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     rep_origin      6627..7215
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      4754..5209
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   4539..4679
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     promoter        5491..5595
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          4417..4457
                     /label="Splicing Donor"
                     /note="color: noColor"
     misc_feature    4461..4494
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     CDS             727..753
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
                     /codon_start=1
                     /transl_table=1
                     /label="HA"
                     /note="color: #cc99b2"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatcta atggaggcaa agagtgtgac
      781 ggaggggaca aggaaggagg tcttccagct atacaagttc ctgtgggttg gcagcgtcgt
      841 gtggatcaaa atggagtgct ttatgtcagt cccagtgggt ctttgttatc ttgcttggag
      901 caggttaaaa catacctgct tactgatgga acatgcaagt gtggcttgga atgtcctctt
      961 attcttccca aggtatttaa ttttgatcct ggagctgctg tgaaacagag aaccgcagaa
     1021 gatgttaagg cagatgaaga tgtcacaaag ctatgcatac ataaaagaaa aattattgca
     1081 gtggccacac ttcataaaag catggaagcc ccacatcctt ctctggtgct caccagtccc
     1141 ggaggaggaa caaatgcaac tccagtagta ccttctcggg cagcaactcc aagatcagta
     1201 agaaataagt ctcatgaagg aattacaaat tctgtaatgc ctgaatgtaa gaatcctttc
     1261 aagttaatga ttggatcatc aaatgccatg ggaaggctat atgtacaaga actgcctgga
     1321 agccaacaac aagaactcca ccctgtctac ccccgacaga gattgggcag cagtgaacat
     1381 ggacagaaat ctccattccg tggcagccat ggaggcctgc ccagcccagc gtcatcaggt
     1441 tcccagatat atggagatgg ttcaatctct ccaaggactg acccacttgg aagtcctgat
     1501 gttttcacaa gaagtaatcc tggttttcat ggagctccca attctagtcc tattcacctg
     1561 aataggactc ctctttctcc accttcagta atgctacatg gttctcctgt acagtcatcc
     1621 tgtgcaatgg ctggaaggac taatatacct ctttccccaa ccttgactac aaagagtcca
     1681 gtaatgaaaa aaccaatgtg taatttttca actaatatgg aaataccacg agcaatgttc
     1741 caccacaaac caccccaagg cccacctccc cctcctccac cttcttgtgc tcttcagaaa
     1801 aagccattaa catctgagaa agatccactt ggcattcttg accctattcc tagtaaacca
     1861 gtgaatcaga accctgttat cattaatcca accagtttcc attcaaatgt ccactctcag
     1921 gtacctatga tgaatgtaag catgcctcct gctgttgttc ctttgccaag taatctccca
     1981 ttgccaactg taaaacctgg tcacatgaat catgggagtc atgtacaaag agttcagcat
     2041 tcagcttcaa cctccctgtc cccttctcca gtgacatccc ccgtgcacat gatggggact
     2101 ggaattggaa ggattgaggc atcgccccaa agatcacgct catcttccac atcatcagat
     2161 catggaaatt tcatgatgcc acctgtagga ccccaggcca cttctagtgg tattaaggtt
     2221 ccacccaggt caccaaggtc aacaataggg tccccaaggc catcaatgcc atcaagccct
     2281 tctaccaagt ccgatggaca tcatcagtac aaggatatcc ctaacccatt aattgctgga
     2341 ataagtaatg tactaaatac cccaagcagt gcagcttttc ctactgcatc tgccggaagt
     2401 agttctgtaa agagtcagcc tggtttgctg ggaatgcctt taaatcagat cttgaaccag
     2461 cacaatgctg cctcctttcc agcaagtagt ttactctcag cagcagccaa agcacagcta
     2521 gcaaatcaaa acaaacttgc tggtaacaac agtagcagca gtagcaattc tggagctgtt
     2581 gccggcagtg gcaacactga aggacatagc actttaaaca ccatgttccc tcctactgcc
     2641 aacatgcttc tcccaacagg tgaagggcaa agtggtcgag cagcactaag agataagctg
     2701 atgtctcagc aaaaagacgc attgcggaaa agaaaacaac cacctacgac agtgttgagt
     2761 ttgctcagac agtctcaaat ggatagttct gcagttccta aacctggacc tgacttgcta
     2821 aggaagcagg gtcagggttc atttcccatc agttcaatgt ctcagttact acagtctatg
     2881 agttgtcaaa gctctcactt gagtagcaat agtaccccgg gttgtggggc ctcaaatact
     2941 gctttgcctt gctctgctaa ccagctgcat tttacagatc ccagtatgaa ctctagtgtt
     3001 cttcagaaca tacctttaag aggggaagcc gtgcactgcc acaatgcaaa cactaacttt
     3061 gttcacagta acagtccagt ccccaaccac catcttgcag gtttaataaa tcagattcag
     3121 gctagcggga actgtgggat gctcagtcag tcgggcatgg ctttaggaaa ttccttacat
     3181 cccaatccac ctcagtcaag aatttcaacg tcctccactc cagtgatacc aaacagcatt
     3241 gttagcagct ataatcaaac aagttctgaa gcaggcggtt caggaccatc atcctccata
     3301 gccatagcgg gcaccaacca ccctgccatc acaaagacaa catctgttct tcaagatggc
     3361 gtcatagtca ccactgcagc tggaaaccca ctgcagagtc agctacccat tgggagtgat
     3421 tttccttttg ttggccagga gcacgcactt cattttccat ccaacagcac ttcaaacaac
     3481 catcttccac accccttgaa ccccagcctc ctcagttctc tacctatctc tttgccagtg
     3541 aatcaacagc atctcctaaa ccagaatcta ttaaatatcc tccagccttc agcaggagaa
     3601 ggcaagtctg agatcaacct ccacccttta ggttttctca acccgaatgt aaacgctgct
     3661 ttagcttttc tctccagtga catggatggg caggtattgc agcctgttca ctttcagctc
     3721 ttagcagcct tgcttcagaa ccaagcccaa gcagctgcca tgcttcccct gccatctttc
     3781 aatctgacca tctcagatct tttgcaacag caaaataccc ctttaccctc attaacacag
     3841 atgacagccc caccagacca tttgccaagc aatcagtcag acaacagccg agctgagacc
     3901 cttttaacca gccccctggg gaacccttta ccaagctttg caggcagtga cactactttt
     3961 aaccccctgt tcctcccagc tgtcaatggg gcctcaggat taatgacctt gaatccccag
     4021 ctgttgggag gtgtcctgaa ctcggcatcg gccaacaccg ctaatcatcc agaggtttcc
     4081 atagcaacct cctcccaggc aaccactacc acaaccacta catcatcagc agtggcagca
     4141 ctgactgtct caacacttgg tgggacagca gtggtgtcaa tggcagaaac attgctgaat
     4201 atatctaata atgctgggaa tacacctggt ccagctaaac tcaacagtaa ctctgtggtg
     4261 ccacagctac ttaaccctct actggggaca ggtctacttg gtgatatgtc atcaataaac
     4321 aatactttga gtaaccatca actgactcat ctacagtcgc tgttaaacaa caatcagatg
     4381 tttcctccaa atcagcaaca gcagcaactt ctccaggtaa gtatcaaggt tacaagacag
     4441 gtttaaggag accaataagc tattattcgt ataggatact ttatacgaag ttattaagtt
     4501 atggagaccg gtgtggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt
     4561 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     4621 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     4681 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     4741 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4801 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4861 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4921 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     4981 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     5041 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat
     5101 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     5161 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     5221 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     5281 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     5341 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     5401 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     5461 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5521 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5581 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5641 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5701 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5761 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5821 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     5881 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5941 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6001 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6061 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6121 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6181 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6241 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6301 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6361 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6421 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6481 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6541 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6601 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6661 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6721 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     6781 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6841 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6901 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6961 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7021 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7081 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7141 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7201 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7261 ccttttgctc acatgt
//
