LOCUS       .                       6248 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    1367..1400
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /label="lox2272-JTZ17"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     CDS             2968..3033
                     /codon_start=1
                     /transl_table=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     promoter        4463..4567
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     repeat_region   3511..3651
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     terminator      3037..3468
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     rep_origin      3726..4181
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     rep_origin      5599..6187
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     CDS             4568..5428
                     /codon_start=1
                     /transl_table=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /label="AmpR"
     CDS             288..1318
                     /codon_start=1
                     /transl_table=1
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /product="site-specific recombinase"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /protein_id=""
                     /label="Cre"
     CDS             1493..2961
                     /codon_start=1
                     /transl_table=1
                     /db_xref="{'GI': '112382224', 'CCDS': 'CCDS95.1', 'GeneID':
                     '473', 'HGNC': 'HGNC:9965', 'MIM': '605226'}"
                     /note="isoform a is encoded by transcript variant 2;
                     atrophin 2; atrophin-1 related protein; atrophin-1 like
                     protein; arginine-glutamic acid dipeptide (RE) repeats"
                     /note="color: #993366"
                     /product="arginine-glutamic acid dipeptide repeats protein
                     isoform a"
                     /translation="GSIAGGSSCPLPTVQIKEEALDDAEEPESPPPPPRSPSPEPTVVD
                     TPSHASQSARFYKHLDRGYNSCARTDLYFMPLAGSKLAKKREEAIEKAKREAEQKAREE
                     REREKEKEKEREREREREREAERAAKASSSAHEGRLSDPQLSGPGHMRPSFEPPPTTIA
                     AVPPYIGPDTPALRTLSEYARPHVMSPTNRNHPFYMPLNPTDPLLAYHMPGLYNVDPTI
                     RERELREREIREREIRERELRERMKPGFEVKPPELDPLHPAANPMEHFARHSALTIPPT
                     AGPHPFASFHPGLNPLERERLALAGPQLRPEMSYPDRLAAERIHAERMASLTSDPLARL
                     QMFNVTPHHHQHSHIHSHLHLHQQDPLHQGSAGPVHPLVDPLTAGPHLARFPYPPGTLP
                     NPLLGQPPHEHEMLRHPVFGTPYPRDLPGAIPPPMSAAHQLQAMHAQSAELQRLAMEQQ
                     WLHGHPHMHGGHLPSQEDYYSRLKKEGDKQL"
                     /gene="RERE"
                     /gene_synonym="ARG; ARP; ATN1L; DNB1; NEDBEH"
                     /protein_id="NP_001036146.1"
                     /label="RERE"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggaggcagc
     1501 atagcggggg ggtcgtcctg cccactcccc accgtccaga tcaaggagga ggctctggac
     1561 gacgctgagg agcctgagag cccccctccc ccaccaagga gcccgtcccc ggagcccact
     1621 gtggtggaca cccccagtca cgccagccag tcagctaggt tctacaaaca cctggaccgg
     1681 ggctacaact cgtgtgcccg gacagacctg tacttcatgc ctctggccgg gtccaagctg
     1741 gccaagaaga gggaggaggc cattgagaag gccaagcgcg aggctgagca gaaagcccga
     1801 gaggagcgag agcgggagaa ggagaaggag aaggagcggg agcgggagcg agagcgggag
     1861 cgcgaggcag agcgggcggc taaggcgtcc agctcagcgc atgaaggtcg cctcagtgac
     1921 ccacagctca gtggtcctgg ccacatgcgg ccatccttcg agccaccacc aaccaccatt
     1981 gctgctgtgc ccccctacat cgggcccgac acacctgccc ttcggactct gagcgagtac
     2041 gcccggcccc acgtcatgtc gcccaccaac cgcaaccacc ccttctacat gccccttaac
     2101 cccacggacc ccctgctggc ctaccacatg cctggcctct acaacgtcga ccccaccatc
     2161 cgcgagcggg agctccggga gcgggagatc cgagagcggg agatccgaga gcgggagctg
     2221 cgggagagga tgaagccggg cttcgaggtg aagcccccag agctggaccc cctgcaccca
     2281 gccgccaacc ccatggagca ctttgcccgg cacagcgccc tcaccatccc cccgaccgcc
     2341 gggccccacc cttttgcttc tttccacccg ggcctgaacc ccttggagag ggagagactg
     2401 gccctggcgg gcccccagct gcggcccgag atgagctacc ctgacagact ggcagccgag
     2461 cgtatccacg cagagcgcat ggcatcgctg accagcgatc ccctggcccg actgcagatg
     2521 ttcaacgtga ctccgcacca tcaccagcac tctcacattc actcccacct ccacctccac
     2581 cagcaggacc ccctccacca aggttcagca ggccccgttc acccgctggt cgaccccctg
     2641 actgccggtc cccacctggc tcgcttcccc tacccgcctg gcactctccc caaccctctg
     2701 cttggacagc ccccacacga gcacgagatg cttcgccacc cagttttcgg caccccctac
     2761 ccccgtgacc tgcctggggc catcccaccc cccatgtcag cagcccacca gctgcaggcc
     2821 atgcatgccc agtcggccga gctgcagaga ctggccatgg agcagcagtg gctgcatgga
     2881 cacccccaca tgcatggtgg ccacctacca agtcaggaag attattacag tcgactgaag
     2941 aaagaaggtg acaagcagtt atgtacagac tacaaagacc atgacggtga ttataaagat
     3001 catgacatcg actacaagga tgacgatgac aagtaactcg agataatcaa cctctggatt
     3061 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     3121 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     3181 cctccttgta taaatcctgg ttagttcttg ccacggcgga actcatcgcc gcctgccttg
     3241 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg tttatttgtg
     3301 aaatttgtga tgctattgct ttatttgtaa ccatctagct ttatttgtga aatttgtgat
     3361 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     3421 attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag atctggagac
     3481 cggtgtggca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
     3541 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     3601 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
     3661 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
     3721 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     3781 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     3841 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     3901 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     3961 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4021 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga
     4081 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4141 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     4201 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     4261 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     4321 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     4381 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     4441 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     4501 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     4561 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     4621 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     4681 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     4741 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     4801 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     4861 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     4921 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     4981 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5041 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     5101 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     5161 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     5221 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     5281 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     5341 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     5401 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     5461 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     5521 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     5581 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     5641 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     5701 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     5761 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     5821 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     5881 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     5941 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6001 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6061 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6121 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6181 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6241 tcacatgt
//
