LOCUS       .                       5278 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             760..2418
                     /codon_start=1
                     /transl_table=1
                     /db_xref="{'GI': '194248070', 'CCDS': 'CCDS93894.1',
                     'GeneID': '8831', 'HGNC': 'HGNC:11497', 'MIM': '603384'}"
                     /product="ras/Rap GTPase-activating protein SynGAP isoform
                     2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     synaptic Ras GTPase activating protein, 135kDa; Ras
                     GTPase-activating protein SynGAP; neuronal RasGAP; synaptic
                     Ras GTPase activating protein 1 homolog; ras/Rap
                     GTPase-activating protein SynGAP; Ras/Rap GTPase-activating
                     protein 1"
                     /note="color: #993366"
                     /translation="MSRSRASIHRGSIPAMSYAPFRDVRGPSMHRTQYVHSPYDRPGWN
                     PRFCIISGNQLLMLDEDEIHPLLIRDRRSESSRNKLLRRTVSVPVEGRPHGEHEYHLGR
                     SRRKSVPGGKQYSMEGAPAAPFRPSQGFLSRRLKSSIKRTKSQPKLDRTSSFRQILPRF
                     RSADHDRARLMQSFKESHSHESLLSPSSAAEALELNLDEDSIIKPVHSSILGQEFCFEV
                     TTSSGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYY
                     CELCLDDMLYARTTSKPRSASGDTVFWGEHFEFNNLPAVRALRLHLYRDSDKKRKKDKA
                     GYVGLVTVPVATLAGRHFTEQWYPVTLPTGSGGSGGMGSGGGGGSGGGSGGKGKGGCPA
                     VRLKARYQTMSILPMELYKEFAEYVTNHYRMLCAVLEPALNVKGKEEVASALVHILQST
                     GKAKDFLSDMAMSEVDRFMEREHLIFRENTLATKAIEEYMRLIGQKYLKDAIGEFIRAL
                     YESEENCEVDPIKCTASSLAEHQANLRMCCELALCK"
                     /gene_synonym="MRD5; RASA1; RASA5; SYNGAP"
                     /gene="SYNGAP1"
                     /protein_id="NP_001123538.1"
                     /label="SYNGAP1"
     CDS             3598..4458
                     /codon_start=1
                     /transl_table=1
                     /product="β-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /label="AmpR"
     rep_origin      4629..5217
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      2756..3211
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   2541..2681
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     promoter        3493..3597
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          2419..2459
                     /label="Splicing Donor"
                     /note="color: noColor"
     misc_feature    2463..2496
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     CDS             727..753
                     /codon_start=1
                     /transl_table=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
                     /label="HA"
                     /note="color: #cc99b2"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatcta tgagcaggtc tcgagcctcc
      781 atccatcggg ggagcatccc cgcgatgtcc tatgccccct tcagagatgt acggggaccc
      841 tctatgcacc gaacccaata cgttcattcc ccgtatgatc gtcctggttg gaaccctcgg
      901 ttctgcatca tctcggggaa ccagctgctc atgctggatg aggatgagat acacccccta
      961 ctgatccggg accggaggag cgagtccagt cgcaacaaac tgctgagacg cacagtctcc
     1021 gtgccggtgg aggggcggcc ccacggcgag catgaatacc acttgggtcg ctcgaggagg
     1081 aagagtgtcc caggggggaa gcagtacagc atggagggtg cccctgctgc gcccttccgg
     1141 ccctcgcaag gcttcctgag ccgacggcta aaaagctcca tcaaacgaac gaagtcacaa
     1201 cccaaacttg accggaccag cagctttcgc cagatcctgc ctcgcttccg aagtgctgac
     1261 catgaccggg cccggctgat gcaaagcttt aaggagtcac actctcatga gtccttgctg
     1321 agtcctagca gtgcagctga ggcattggag ctcaacttgg atgaagattc cattatcaag
     1381 ccagtgcaca gctccatcct gggccaggag ttctgttttg aggtaacaac ttcatcagga
     1441 acaaaatgct ttgcctgtcg gtctgcggcc gaaagagaca aatggattga gaatctgcag
     1501 cgggcagtaa agcccaacaa ggacaacagc cgccgggtag acaatgtgct aaagctgtgg
     1561 atcatagagg cccgggagct gccccccaag aagcggtact actgtgagct ctgcctggat
     1621 gacatgctgt atgcacgcac cacctccaag ccccgctctg cctctgggga caccgtcttc
     1681 tggggcgagc acttcgagtt taacaacctg ccggctgtcc gtgccctgcg gctgcatctg
     1741 taccgtgact cagacaaaaa gcgcaagaag gacaaggcag gctatgtcgg cctggtgact
     1801 gtgccagtgg ccaccctggc tgggcgccac ttcacagagc agtggtaccc tgtaaccctg
     1861 ccaacaggca gtgggggatc tgggggcatg ggttcgggag ggggaggggg ctcggggggt
     1921 ggctcagggg gcaagggcaa aggaggttgc ccggctgtgc ggctgaaagc acgttaccag
     1981 acaatgagca tcttgcccat ggagctatat aaagagtttg cagagtatgt caccaaccat
     2041 tatcggatgc tgtgtgcagt cttggagccc gccctgaatg tcaaaggcaa ggaggaggtt
     2101 gccagtgcac tagttcacat cctgcagagt acaggcaagg ccaaggactt cctttcagac
     2161 atggccatgt ctgaggtaga ccggttcatg gaacgggagc acctcatatt ccgcgagaac
     2221 acgcttgcca ctaaagccat agaagagtat atgagactga ttggtcagaa atacctcaag
     2281 gatgccattg gagaattcat ccgtgctctg tatgaatctg aggaaaactg cgaggtagac
     2341 cctatcaagt gcacagcatc cagtttggca gagcaccagg ccaacctgcg aatgtgctgt
     2401 gagttggccc tgtgcaaggt aagtatcaag gttacaagac aggtttaagg agaccaataa
     2461 gctattattc gtataggata ctttatacga agttattaag ttatggagac cggtgtggca
     2521 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
     2581 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     2641 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
     2701 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     2761 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     2821 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     2881 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     2941 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     3001 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     3061 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg
     3121 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     3181 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     3241 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     3301 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     3361 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     3421 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     3481 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     3541 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     3601 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     3661 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     3721 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     3781 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     3841 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     3901 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     3961 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     4021 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     4081 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     4141 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     4201 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     4261 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     4321 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     4381 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     4441 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     4501 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     4561 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     4621 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     4681 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     4741 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     4801 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4861 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4921 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4981 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5041 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5101 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5161 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     5221 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
