LOCUS       .                       6996 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1493..3709
                     /product="ras/Rap GTPase-activating protein SynGAP isoform
                     2"
                     /translation="VVNSHCVFPRELKEVFASWRLRCAERGREDIADRLISASLFLRFL
                     CPAIMSPSLFGLMQEYPDEQTSRTLTLIAKVIQNLANFSKFTSKEDFLGFMNEFLELEW
                     GSMQQFLYEISNLDTLTNSSSFEGYIDLGRELSTLHALLWEVLPQLSKEALLKLGPLPR
                     LLNDISTALRNPNIQRQPSRQSERPRPQPVVLRGPSAEMQGYMMRDLNSSMDMARLPSP
                     TKEKPPPPPPGGGKDLFYVSRPPLARSSPAYCTSSSDITEPEQKMLSVNKSVSMLDLQG
                     DGPGGRLNSSSVSNLAAVGDLLHSSQASLTAALGLRPAPAGRLSQGSGSSITAAGMRLS
                     QMGVTTDGVPAQQLRIPLSFQNPLFHMAADGPGPPGGHGGGGGHGPPSSHHHHHHHHHH
                     RGGEPPGDTFAPFHGYSKSEDLSSGVPKPPAASILHSHSYSDEFGPSGTDFTRRQLSLQ
                     DNLQHMLSPPQITIGPQRPAPSGPGGGSGGGSGGGGGGQPPPLQRGKSQQLTVSAAQKP
                     RPSSGNLLQSPEPSYGPARPRQQSLSKEGSIGGSGGSGGGGGGGLKPSITKQHSQTPST
                     LNPTMPASERTVAWVSNMPHLSADIESAHIEREEYKLKEYSKSMDESRLDREYEEEIHS
                     LKERLHMSNRKLEEYERRLLSQEEQTSKILMQYQARLEQSEKRLRQQQAEKDSQIKSII
                     GRLMLVEEELRRDHPAMAEPLPEPKKRLLDAQRGSFPPWVQQTRV"
                     /gene="SYNGAP1"
                     /transl_table=1
                     /note="isoform 2 is encoded by transcript variant 2;
                     synaptic Ras GTPase activating protein, 135kDa; Ras
                     GTPase-activating protein SynGAP; neuronal RasGAP; synaptic
                     Ras GTPase activating protein 1 homolog; ras/Rap
                     GTPase-activating protein SynGAP; Ras/Rap GTPase-activating
                     protein 1"
                     /note="color: #993366"
                     /protein_id="NP_001123538.1"
                     /codon_start=1
                     /db_xref="{'GI': '194248070', 'CCDS': 'CCDS93894.1',
                     'GeneID': '8831', 'HGNC': 'HGNC:11497', 'MIM': '603384'}"
                     /gene_synonym="MRD5; RASA1; RASA5; SYNGAP"
                     /label="SYNGAP1"
     CDS             5316..6176
                     /product="β-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /transl_table=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /codon_start=1
                     /label="AmpR"
     rep_origin      6347..6935
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     rep_origin      4474..4929
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     terminator      3785..4216
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   4259..4399
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     promoter        5211..5315
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     CDS             3716..3781
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /transl_table=1
                     /codon_start=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     CDS             288..1318
                     /product="site-specific recombinase"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /transl_table=1
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /protein_id=""
                     /codon_start=1
                     /label="Cre"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     protein_bind    1367..1400
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /label="lox2272-JTZ17"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtggtcaa
     1501 ctcccactgc gtgttcccga gggagctgaa ggaggtgttt gcttcgtggc ggctgcgctg
     1561 cgcagagcga ggccgggagg acatcgcaga caggcttatc agcgcctcac tcttcctgcg
     1621 cttcctctgc ccagcgatta tgtcgcccag tctctttggg cttatgcagg agtacccaga
     1681 tgagcagacc tcacgaaccc tcaccctcat tgccaaggtc atccagaacc tggccaactt
     1741 ttccaagttt acctcaaagg aggactttct gggcttcatg aatgagtttc tggagctgga
     1801 atggggttcc atgcagcagt ttttgtatga gatctccaat ctggacacgc taaccaacag
     1861 cagtagcttt gagggttaca tcgacttggg ccgagagctc tccacactgc atgccctact
     1921 ctgggaggtg ctgccccagc tcagcaagga agccctcctg aagctgggtc cactgccccg
     1981 gctcctcaac gacatcagca cagctctgag gaaccccaac atccaaaggc agccaagccg
     2041 ccagagtgag cggccccggc ctcagcctgt ggtactgcgg gggccatcgg ctgagatgca
     2101 gggctacatg atgcgggacc tcaacagctc tatggacatg gctcgcctcc cctccccaac
     2161 caaggaaaag ccacccccac caccgcctgg tggtggtaaa gacctgttct atgtaagccg
     2221 tccacccctg gcccgttcct caccagcata ctgcacgagc agctcggaca tcacagagcc
     2281 agagcagaag atgctgagtg tcaacaagag tgtgtccatg ctggacttac agggtgatgg
     2341 gcctggtggc cgcctcaaca gcagcagtgt ttcgaacctg gcggccgtag gggacctgct
     2401 gcactcaagc caggcctcgc tgacagcagc cttggggcta cggcctgcgc ctgccggacg
     2461 cctctcccag gggagtggct catccatcac ggcggctggc atgcgcctca gccagatggg
     2521 tgtcaccaca gacggtgtcc ctgcccagca actgcgaatc cccctctcct tccagaaccc
     2581 tctcttccac atggctgctg atgggccagg tcccccaggc ggccatggag ggggcggtgg
     2641 ccatggccca ccttcctccc atcaccacca ccaccaccat caccaccacc gaggtggaga
     2701 gccccctggg gacacctttg ccccattcca tggctatagc aagagtgagg acctctcttc
     2761 cggggtcccc aagccccctg ctgcctccat ccttcatagc cacagctaca gtgatgagtt
     2821 tggaccctct ggcactgact tcacccgtcg gcagctttca ctccaggaca acctgcagca
     2881 catgctgtcc cctccccaga tcaccattgg tccccagagg ccagccccct cagggcctgg
     2941 aggtgggagc ggtgggggca gcggtggggg tggcgggggc cagccgcctc cattgcagag
     3001 gggcaagtct cagcagttga cagtcagcgc agcccagaaa ccccggccat ccagcgggaa
     3061 tctattgcag tccccagagc caagttatgg ccccgcccgt ccacggcaac agagcctcag
     3121 caaggagggc agcattgggg gcagcggggg cagcggtggc ggagggggtg gggggctgaa
     3181 gccctccatc accaagcagc attctcagac accatccaca ttgaacccca caatgccagc
     3241 ctctgagcgg acagtggcct gggtctccaa catgcctcac ctgtcggctg acatcgagag
     3301 tgcccacatc gagcgggaag agtacaagct caaggagtac tcaaaatcga tggatgagag
     3361 ccggctggat agggagtacg aggaggagat tcactcactg aaagagcggc tgcacatgtc
     3421 caaccggaag ctggaagagt atgagcggag gctgctgtcc caggaagaac aaaccagcaa
     3481 aatcctgatg cagtatcagg cccgactgga gcagagtgag aagaggctaa ggcagcagca
     3541 ggcagagaag gattcccaga tcaagagcat cattggcagg ctgatgctgg tggaggagga
     3601 gctgcgccgg gaccaccccg ccatggctga gccgctgcca gaacccaaga agaggctgct
     3661 cgacgctcag agaggcagct tccccccttg ggtccaacaa acccgcgtgt gtacagacta
     3721 caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa
     3781 gtaactcgag ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     3841 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     3901 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt agttcttgcc
     3961 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4021 actgacaatt ccgtggtgtt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     4081 atctagcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     4141 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat
     4201 gtgggaggtt ttttaaagat ctggagaccg gtgtggcacg tgcggaccga gcggccgcag
     4261 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     4321 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
     4381 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     4441 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
     4501 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4561 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4621 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4681 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4741 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4801 tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     4861 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     4921 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     4981 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     5041 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5101 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     5161 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5221 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5281 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5341 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5401 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5461 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5521 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5581 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5641 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5701 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5761 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5821 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5881 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5941 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6001 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6061 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6121 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6181 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6241 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6301 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6361 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6421 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6481 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6541 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6601 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6661 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6721 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6781 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6841 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6901 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6961 ggttcctggc cttttgctgg ccttttgctc acatgt
//
