LOCUS       .                       6159 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    1367..1400
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /label="lox2272-JTZ17"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     CDS             2879..2944
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /codon_start=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     promoter        4374..4478
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     repeat_region   3422..3562
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     terminator      2948..3379
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     rep_origin      3637..4092
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     rep_origin      5510..6098
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             4479..5339
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /gene="bla"
                     /product="β-lactamase"
                     /transl_table=1
                     /codon_start=1
                     /label="AmpR"
     CDS             288..1318
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /product="site-specific recombinase"
                     /transl_table=1
                     /codon_start=1
                     /protein_id=""
                     /label="Cre"
     CDS             1493..2872
                     /translation="VGSASLSSGRLHKRKHKHKHKHKEDRILGTHDNLSGLFAGKATGF
                     SSHILSERLSSADKELPLVSEKNKHKEKQKHQHSEAGHKASKNNFEVDTLSTLSLSDAQ
                     HWTQAKEKGDLSSEPVDSCTKRYSGSGGDGGSTRSENLDVFSEMNPSNDKWDSDVSGSK
                     RRSYEGFGTYREKDIQAFKMNRKERSSYDSSMSPGMPSPHLKVDQTAVHSKNEGSVPTM
                     MTRKKPAAVDSVTIPPAPVLSLLAASAATSDAVGSSLKKRFKRREIEAIQCEVRKMCNY
                     TKILSTKKNLDHVNKILKAKRLQRQSKTGNNFVKKRRGRPRKQPTQFDEDSRDQMPVLE
                     KCIDLPSKRGQKPSLSPLVLEPAASQDTIMATIEAVIHMAREAPPLPPPPPPPLPPPPP
                     PPLPPPPPLPKTPRGGKRKHKPQAPAQPPQQSPPQQPLPQEEEVKAKRQRKSRGSESEV
                     LP"
                     /note="isoform a is encoded by transcript variant 1;
                     SET-binding protein"
                     /note="color: #993366"
                     /db_xref="{'GI': '194294554', 'CCDS': 'CCDS11923.2',
                     'GeneID': '26040', 'HGNC': 'HGNC:15573', 'MIM': '611060'}"
                     /gene="SETBP1"
                     /product="SET-binding protein isoform a"
                     /gene_synonym="MRD29; SEB"
                     /transl_table=1
                     /codon_start=1
                     /protein_id="NP_056374.2"
                     /label="SETBP1"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtaggcag
     1501 tgccagtctg tccagtggtc ggctccataa gaggaaacac aaacacaagc ataagcacaa
     1561 ggaagaccgg atcctaggga cccatgacaa cctgagtggt ctttttgcag gcaaagccac
     1621 aggcttctcc agccacatcc tgagcgagcg gctgagtagc gcagacaaag agctcccgct
     1681 ggtgagtgag aagaacaagc ataaggagaa acagaagcac cagcacagcg aagccggcca
     1741 caaagcttct aagaacaact ttgaggtgga caccctgtct acactgtcac tttccgacgc
     1801 ccagcattgg acacaggcca aggaaaaagg agacttgagc agtgagcctg tggactcatg
     1861 cacgaaaaga tactctggca gtggcgggga tggtggcagc acgagatcag agaacctgga
     1921 cgtgttcagt gaaatgaacc cttcgaatga caagtgggac agtgacgtga gtgggagtaa
     1981 aaggaggagc tatgaaggct ttggaacgta cagggaaaag gacatccaag ccttcaagat
     2041 gaaccgcaag gagagaagtt cttatgactc ctccatgtct ccagggatgc caagtcccca
     2101 cttaaaagtg gaccagacag cagtgcatag taagaacgaa ggctcagtgc ccaccatgat
     2161 gaccaggaag aagccagccg cagttgacag tgttacaatt ccaccagccc cagtgttatc
     2221 tctccttgct gcatctgcag caacgtcgga tgcagtcggc tcctccctga agaagaggtt
     2281 caagcggcgg gagatcgaag ccatccagtg cgaagtgcgg aagatgtgca actacaccaa
     2341 gatcctgtcc accaagaaga acctggacca cgtgaacaag atcctgaagg ccaagcggct
     2401 gcagagacaa tcaaaaacag gcaacaactt cgtgaagaag aggcgcgggc gtcccaggaa
     2461 gcagcccacc cagttcgatg aggactccag agaccaaatg ccggtgctgg aaaaatgcat
     2521 cgacctgccc agcaaaagag gccagaagcc cagcctgagc ccgctggtgc tggagcccgc
     2581 cgccagccaa gacaccatca tggccaccat cgaggcggtc atccacatgg cccgggaggc
     2641 gccgcccctg cccccgccac cgccgccgcc cctgccgcca ccgccgccac cacccctgcc
     2701 cccgccaccc cctctaccca agaccccccg aggcggaaag aggaaacaca aaccgcaggc
     2761 ccccgctcag cccccacagc agtcgccccc gcagcagccc cttccccagg aagaggaggt
     2821 gaaagccaaa aggcagagga agtcccgagg gagtgagagc gaggtccttc cctgtacaga
     2881 ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga
     2941 caagtaactc gagataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     3001 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     3061 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttagttctt
     3121 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3181 ggcactgaca attccgtggt gtttatttgt gaaatttgtg atgctattgc tttatttgta
     3241 accatctagc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     3301 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     3361 gatgtgggag gttttttaaa gatctggaga ccggtgtggc acgtgcggac cgagcggccg
     3421 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     3481 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     3541 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
     3601 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     3661 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     3721 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     3781 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     3841 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     3901 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     3961 cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct
     4021 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     4081 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     4141 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     4201 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     4261 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     4321 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     4381 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4441 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4501 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4561 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4621 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4681 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4741 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4801 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4861 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4921 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4981 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5041 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5101 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5161 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5221 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5281 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5341 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5401 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5461 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5521 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5581 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5641 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5701 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5761 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5821 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5881 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5941 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6001 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6061 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6121 tacggttcct ggccttttgc tggccttttg ctcacatgt
//
