LOCUS       .                       6621 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             727..753
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /codon_start=1
                     /label="HA"
                     /note="color: #cc99b2"
     misc_feature    3806..3839
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     intron          3762..3802
                     /label="Splicing Donor"
                     /note="color: noColor"
     promoter        4836..4940
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   3884..4024
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     rep_origin      4099..4554
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      5972..6560
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             4941..5801
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /gene="bla"
                     /product="β-lactamase"
                     /transl_table=1
                     /codon_start=1
                     /label="AmpR"
     CDS             760..3761
                     /translation="HRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGR
                     CSASIGQPPLPAPLPPSHGSSSGHPSKPYYAPGAPTPRPLHGKLESLHGCVQALLREPA
                     QPGLWEQLGQLYESEHDSEEATRCYHSALRYGGSFAELGPRIGRLQQAQLWNFHTGSCQ
                     HRAKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGL
                     SPGGKRRRGCNSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPLPGLATSPPFQLTKPGLW
                     STLHGDAWGPERKGSAPPERQEQRHSLPHPYPYPAPAYTAHPPGHRLVPAAPPGPGPRP
                     PGAESHGCLPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSS
                     SSSSSSNTGLRGVEPNPGIPGADHYQTPALEVSHHGRLGPSAHSSRKPFLGAPAATPHL
                     SLPPGPSSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGTEGPPRPAPPP
                     LPHREGFLGPPASRFSVGTQDSHTPPTPPTPTTSSSNSNSGSHSSSPAGPVSFPPPPYL
                     ARSIDPLPRPPSPAQNPQDPPLVPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPK
                     TPEVGPGPPPGPLSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGL
                     ANIMKMLDESIRKEEEQQQHEAGVAPQPPLKEPFASLQSPFPTDTAPTTTAPAVAVTTT
                     TTTTTTTTATQEEEKKPPPALPPPPPLAKFPPPSQPQPPPPPPPSPASLLKSLASVLEG
                     QKYCYRGTGAAVSTRPGPLPTTQYSPGPPSGATALPPTSAAPSAQGSPQPSASSSSQFS
                     TSGGPWARERRAGEEPVPGPMTPTQPPPPLSLPPARSESEVLEEISRACETLVERVGRS
                     ATDPADPVDTAEPADSGTERLLPPAQAKEEAGGVAAVSGSCKRRQKEHQKEHRRHRRAC
                     KDSVGRRPREG"
                     /note="isoform 2 is encoded by transcript variant 2;
                     jumonji domain containing 3, histone lysine demethylase;
                     lysine (K)-specific demethylase 6B; lysine-specific
                     demethylase 6B; jmjC domain-containing protein 3; jumonji
                     domain-containing protein 3; [histone
                     H3]-trimethyl-L-lysine(27) demethylase 6B"
                     /note="color: #993366"
                     /db_xref="{'GI': '1145994701', 'CCDS': 'CCDS86569.1',
                     'GeneID': '23135', 'HGNC': 'HGNC:29012', 'MIM': '611577'}"
                     /EC_number="1.14.11.68"
                     /gene="KDM6B"
                     /product="lysine-specific demethylase 6B isoform 2"
                     /gene_synonym="JMJD3; NEDCFSA"
                     /transl_table=1
                     /codon_start=1
                     /protein_id="NP_001335645.1"
                     /label="KDM6B"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatctc atcgggcagt ggaccctcca
      781 ggggcccgcg ctgcacggga agcctttgcc cttgggggcc tgagctgtgc tggggcctgg
      841 agctcctgcc cgcctcatcc ccctcctcgt agcgcatggc tgcctggagg cagatgctca
      901 gccagcattg ggcagccccc gcttcctgct cccctacccc cttcacatgg cagtagttct
      961 gggcacccca gcaaaccata ttatgctcca ggggcgccca ctccaagacc cctccatggg
     1021 aagctggaat ccctgcatgg ctgtgtgcag gcattgctcc gggagccagc ccagccaggg
     1081 ctttgggaac agcttgggca actgtacgag tcagagcacg atagtgagga ggccacacgc
     1141 tgctaccaca gcgcccttcg atacggagga agcttcgctg agctggggcc ccgcattggc
     1201 cgactgcagc aggcccagct ctggaacttt catactggct cctgccagca ccgagccaag
     1261 gtcctgcccc cactggagca agtgtggaac ttgctacacc ttgagcacaa acggaactat
     1321 ggagccaagc ggggaggtcc cccggtgaag cgagctgctg aacccccagt ggtgcagcct
     1381 gtgcctcctg cagcactctc aggcccctca ggggaggagg gcctcagccc tggaggcaag
     1441 cgaaggagag gctgcaactc tgaacagact ggccttcccc cagggctgcc actgcctcca
     1501 ccaccattac caccaccacc accaccacca ccaccaccac caccacccct gcctggcctg
     1561 gctaccagcc ccccatttca gctaaccaag ccagggctgt ggagtaccct gcatggagat
     1621 gcctggggcc cagagcgcaa gggttcagca cccccagagc gccaggagca gcggcactcg
     1681 ctgcctcacc catatccata cccagctcca gcgtacaccg cgcacccccc tggccaccgg
     1741 ctggtcccgg ctgctccccc aggcccaggc ccccgccccc caggagcaga gagccatggc
     1801 tgcctgcctg ccacccgtcc ccccggaagt gaccttagag agagcagagt tcagaggtcg
     1861 cggatggact ccagcgtttc accagcagca accaccgcct gcgtgcctta cgccccttcc
     1921 cggccccctg gcctccccgg caccaccacc agcagcagca gtagcagcag cagcaacact
     1981 ggtctccggg gcgtggagcc gaacccaggc attcccggcg ctgaccatta ccaaactccc
     2041 gcgctggagg tctctcacca tggccgcctg gggccctcgg cacacagcag tcggaaaccg
     2101 ttcttggggg ctcccgctgc cactccccac ctatccctgc cacctggacc ttcctcaccc
     2161 cctccacccc cctgtccccg cctcttacgc cccccaccac cccctgcctg gttgaagggt
     2221 ccggcctgcc gggcagcccg agaggatgga gagatcttag aagagctctt ctttgggact
     2281 gagggacccc cccgccctgc cccaccaccc ctcccccatc gcgagggctt cttggggcct
     2341 ccggcctccc gcttttctgt gggcactcag gattctcaca cccctcccac tcccccaacc
     2401 ccaaccacca gcagtagcaa cagcaacagt ggcagccaca gcagcagccc tgctgggcct
     2461 gtgtcctttc ccccaccacc ctatctggcc agaagtatag acccccttcc ccggcctccc
     2521 agcccagcac agaaccccca ggacccacct cttgtacccc tgactcttgc cctgcctcca
     2581 gcccctcctt cctcctgcca ccaaaatacc tcaggaagct tcaggcgccc ggagagcccc
     2641 cggcccaggg tctccttccc aaagaccccc gaggtggggc cggggccacc cccaggcccc
     2701 ctgagtaaag ccccccagcc tgtgccgccc ggggttgggg agctgcctgc ccgaggccct
     2761 cgactctttg attttccccc cactccgctg gaggaccagt ttgaggagcc agccgaattc
     2821 aagatcctac ctgatgggct ggccaacatc atgaagatgc tggacgaatc cattcgcaag
     2881 gaagaggaac agcaacaaca cgaagcaggc gtggcccccc aacccccgct gaaggagccc
     2941 tttgcatctc tgcagtctcc tttccccacc gacacagccc ccaccactac tgctcctgct
     3001 gtcgccgtca ccaccaccac caccaccacc accaccacca cggccaccca ggaagaggag
     3061 aagaagccac caccagccct accaccacca ccgcctctag ccaagttccc tccaccctct
     3121 cagccacagc caccaccacc cccacccccc agcccggcca gcctgctcaa atccttggcc
     3181 tccgtgctgg agggacaaaa gtactgttat cgggggactg gagcagctgt ttccacccgg
     3241 cctgggccct tgcccaccac tcagtattcc cctggccccc catcaggtgc taccgccctg
     3301 ccgcccacct cagcggcccc tagcgcccag ggctccccac agccctctgc ttcctcgtca
     3361 tctcagttct ctacctcagg cgggccctgg gcccgggagc gcagggcggg cgaagagcca
     3421 gtcccgggcc ccatgacccc cacccaaccg cccccacccc tatctctgcc ccctgctcgc
     3481 tctgagtctg aggtgctaga agagatcagc cgggcttgcg agacccttgt ggagcgggtg
     3541 ggccggagtg ccactgaccc agccgaccca gtggacacag cagagccagc ggacagtggg
     3601 actgagcgac tgctgccccc cgcacaggcc aaggaggagg ctggcggggt ggcggcagtg
     3661 tcaggcagct gtaagcggcg acagaaggag catcagaagg agcatcggcg gcacaggcgg
     3721 gcctgtaagg acagtgtggg tcgtcggccc cgtgagggca ggtaagtatc aaggttacaa
     3781 gacaggttta aggagaccaa taagctatta ttcgtatagg atactttata cgaagttatt
     3841 aagttatgga gaccggtgtg gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg
     3901 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     3961 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     4021 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     4081 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4141 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4201 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4261 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     4321 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4381 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     4441 gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     4501 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     4561 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     4621 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     4681 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     4741 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     4801 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4861 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4921 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4981 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5041 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5101 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5161 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5221 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5281 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5341 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5401 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5461 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5521 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5581 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5641 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5701 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5761 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5821 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5881 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5941 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6001 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6061 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     6121 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6181 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6241 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6301 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6361 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6421 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6481 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6541 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6601 gctggccttt tgctcacatg t
//
