LOCUS       .                       6715 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1493..3416
                     /translation="AKAKAKVPKEKSRRVLGNLDLQSEEIQGREKSRPDLGGASKAKPP
                     TAPAPPSAPAPSAQPTPPSASVPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIR
                     LIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASRNAKVKGKFRESYLSPAQS
                     VKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRL
                     NLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQY
                     QASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKP
                     QLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNI
                     NIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGD
                     LVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSW
                     NVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNEC
                     DVEVFNILFVTSENGSRNTYLVHCEGCARRRSAGLQGVVVLEQYRTEELAQAYDAFTLA
                     PASTSR"
                     /note="isoform 2 is encoded by transcript variant 2;
                     jumonji domain containing 3, histone lysine demethylase;
                     lysine (K)-specific demethylase 6B; lysine-specific
                     demethylase 6B; jmjC domain-containing protein 3; jumonji
                     domain-containing protein 3; [histone
                     H3]-trimethyl-L-lysine(27) demethylase 6B"
                     /note="color: #993366"
                     /db_xref="{'GI': '1145994701', 'CCDS': 'CCDS86569.1',
                     'GeneID': '23135', 'HGNC': 'HGNC:29012', 'MIM': '611577'}"
                     /EC_number="1.14.11.68"
                     /gene="KDM6B"
                     /product="lysine-specific demethylase 6B isoform 2"
                     /gene_synonym="JMJD3; NEDCFSA"
                     /transl_table=1
                     /codon_start=1
                     /protein_id="NP_001335645.1"
                     /label="KDM6B"
     CDS             288..1318
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /product="site-specific recombinase"
                     /transl_table=1
                     /codon_start=1
                     /protein_id=""
                     /label="Cre"
     CDS             5035..5895
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /gene="bla"
                     /product="β-lactamase"
                     /transl_table=1
                     /codon_start=1
                     /label="AmpR"
     rep_origin      6066..6654
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     rep_origin      4193..4648
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     terminator      3504..3935
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   3978..4118
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     promoter        4930..5034
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     CDS             3423..3488
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /codon_start=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     protein_bind    1367..1400
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /label="lox2272-JTZ17"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac agggcaaagg
     1501 ccaaggccaa ggtccccaaa gaaaagagcc gccgggtgct ggggaacctg gacctgcaga
     1561 gcgaggagat ccagggtcgt gagaagtccc ggcccgatct tggcggggcc tccaaggcca
     1621 agccacccac agctccagcc cctccatcag ctcctgcacc ttctgcccag cccacacccc
     1681 cgtcagcctc tgtccctgga aagaaggctc gggaggaagc cccagggcca ccgggtgtca
     1741 gccgggccga catgctgaag ctgcgctcac ttagtgaggg gccccccaag gagctgaaga
     1801 tccggctcat caaggtagag agtggtgaca aggagacctt tatcgcctct gaggtggaag
     1861 agcggcggct gcgcatggca gacctcacca tcagccactg tgctgctgac gtcgtgcgcg
     1921 ccagcaggaa tgccaaggtg aaagggaagt ttcgagagtc ctacctttcc cctgcccagt
     1981 ctgtgaaacc gaagatcaac actgaggaga agctgccccg ggaaaaactc aaccccccta
     2041 cacccagcat ctatctggag agcaaacggg atgccttctc acctgtcctg ctgcagttct
     2101 gtacagaccc tcgaaatccc atcacagtga tccggggcct ggcgggctcc ctgcggctca
     2161 acttgggcct cttctccacc aagaccctgg tggaagcgag tggcgaacac accgtggaag
     2221 ttcgcaccca ggtgcagcag ccctcagatg agaactggga tctgacaggc actcggcaga
     2281 tctggccttg tgagagctcc cgttcccaca ccaccattgc caagtacgca cagtaccagg
     2341 cctcatcctt ccaggagtct ctgcaggagg agaaggagag tgaggatgag gagtcagagg
     2401 agccagacag caccactgga acccctccta gcagcgcacc agacccgaag aaccatcaca
     2461 tcatcaagtt tggcaccaac atcgacttgt ctgatgctaa gcggtggaag ccccagctgc
     2521 aggagctgct aaagctgccc gccttcatgc gggtaacatc cacgggcaac atgctgagcc
     2581 acgtgggcca caccatcctg ggcatgaaca cggtgcagct gtacatgaag gtgcccggca
     2641 gccgaacgcc aggccaccag gagaataaca acttctgctc cgtcaacatc aacattggcc
     2701 caggcgactg cgagtggttc gcggtgcacg agcactactg ggagaccatc agcgctttct
     2761 gtgatcggca cggcgtggac tacttgacgg gttcctggtg gccaatcctg gatgatctct
     2821 atgcatccaa tattcctgtg taccgcttcg tgcagcgacc cggagacctc gtgtggatta
     2881 atgcggggac tgtgcactgg gtgcaggcca ccggctggtg caacaacatt gcctggaacg
     2941 tggggcccct caccgcctat cagtaccagc tggccctgga acgatacgag tggaatgagg
     3001 tgaagaacgt caaatccatc gtgcccatga ttcacgtgtc atggaacgtg gctcgcacgg
     3061 tcaaaatcag cgaccccgac ttgttcaaga tgatcaagtt ctgcctgctg cagtccatga
     3121 agcactgcca ggtgcaacgc gagagcctgg tgcgggcagg gaagaaaatc gcttaccagg
     3181 gccgtgtcaa ggacgagcca gcctactact gcaacgagtg cgatgtggag gtgtttaaca
     3241 tcctgttcgt gacaagtgag aatggcagcc gcaacacgta cctggtacac tgcgagggct
     3301 gtgcccggcg ccgcagcgca ggcctgcagg gcgtggtggt gctggagcag taccgcactg
     3361 aggagctggc tcaggcctac gacgccttca cgctggcccc agccagcacg tcgcgatgta
     3421 cagactacaa agaccatgac ggtgattata aagatcatga catcgactac aaggatgacg
     3481 atgacaagta agaattctga tgactcgaga taatcaacct ctggattaca aaatttgtga
     3541 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     3601 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     3661 atcctggtta gttcttgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     3721 aggggctcgg ctgttgggca ctgacaattc cgtggtgttt atttgtgaaa tttgtgatgc
     3781 tattgcttta tttgtaacca tctagcttta tttgtgaaat ttgtgatgct attgctttat
     3841 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     3901 ttcaggttca gggggagatg tgggaggttt tttaaagatc tggagaccgg tgtggcacgt
     3961 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     4021 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
     4081 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
     4141 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
     4201 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4261 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4321 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4381 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     4441 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4501 ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt
     4561 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4621 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
     4681 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     4741 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     4801 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     4861 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     4921 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     4981 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     5041 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     5101 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     5161 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     5221 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     5281 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5341 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     5401 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     5461 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5521 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     5581 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5641 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5701 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5761 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5821 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     5881 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     5941 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6001 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6061 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6121 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6181 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     6241 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6301 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6361 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6421 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6481 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6541 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6601 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6661 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
