LOCUS       .                       6658 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             727..753
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
                     /transl_table=1
                     /label="HA"
                     /note="color: #cc99b2"
     misc_feature    3843..3876
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     intron          3799..3839
                     /label="Splicing Donor"
                     /note="color: noColor"
     promoter        4873..4977
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   3921..4061
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     rep_origin      4136..4591
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      6009..6597
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     CDS             4978..5838
                     /codon_start=1
                     /product="β-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /transl_table=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /label="AmpR"
     CDS             760..3798
                     /codon_start=1
                     /product="transcription activator BRG1 isoform A"
                     /translation="STPDPPLGGTPRPGPSPGPGPSPGAMLGPSPGPSPGSAHSMMGPS
                     PGPPSAGHPIPTQGPGGYPQDNMHQMHKPMESMHEKGMSDDPRYNQMKGMGMRSGGHAG
                     MGPPPSPMDQHSQGYPSPLGGSEHASSPVPASGPSSGPQMSSGPGGAPLDGADPQALGQ
                     QNRGPTPFNQNQLHQLRAQIMAYKMLARGQPLPDHLQMAVQGKRPMPGMQQQMPTLPPP
                     SVSATGPGPGPGPGPGPGPGPAPPNYSRPHGMGGPNMPPPGPSGVPPGMPGQPPGGPPK
                     PWPEGPMANAAAPTSTPQKLIPPQPTGRPSPAPPAVPPAASPVMPPQTQSPGQPAQPAP
                     MVPLHQKQSRITPIQKPRGLDPVEILQEREYRLQARIAHRIQELENLPGSLAGDLRTKA
                     TIELKALRLLNFQRQLRQEVVVCMRRDTALETALNAKAYKRSKRQSLREARITEKLEKQ
                     QKIEQERKRRQKHQEYLNSILQHAKDFKEYHRSVTGKIQKLTKAVATYHANTEREQKKE
                     NERIEKERMRRLMAEDEEGYRKLIDQKKDKRLAYLLQQTDEYVANLTELVRQHKAAQVA
                     KEKKKKKKKKKAENAEGQTPAIGPDGEPLDETSQMSDLPVKVIHVESGKILTGTDAPKA
                     GQLEAWLEMNPGYEVAPRSDSEESGSEEEEEEEEEEQPQAAQPPTLPVEEKKKIPDPDS
                     DDVSEVDARHIIENAKQDVDDEYGVSQALARGLQSYYAVAHAVTERVDKQSALMVNGVL
                     KQYQIKGLEWLVSLYNNNLNGILADEMGLGKTIQTIALITYLMEHKRINGPFLIIVPLS
                     TLSNWAYEFDKWAPSVVKVSYKGSPAARRAFVPQLRSGKFNVLLTTYEYIIKDKHILAK
                     IRWKYMIVDEGHRMKNHHCKLTQVLNTHYVAPRRLLLTGTPLQNKLPELWALLNFLLPT
                     IFKSCSTFEQWFNAPFAMTGEKVDLNEEETILIIRRLHKVLRPFLLRRLKKEVEAQLPE
                     KVEYVIKCDMSALQRVLYRHMQAK"
                     /gene_synonym="BAF190; BAF190A; BRG1; CSS4; hSNF2b; MRD16;
                     RTPS2; SNF2; SNF2-beta; SNF2L4; SNF2LB; SWI2"
                     /protein_id="NP_001374212.1"
                     /gene="SMARCA4"
                     /transl_table=1
                     /note="isoform A is encoded by transcript variant 10;
                     sucrose nonfermenting-like 4; mitotic growth and
                     transcription activator; BRM/SWI2-related gene 1; homeotic
                     gene regulator; nuclear protein GRB1; brahma protein-like
                     1; SNF2-like 4; global transcription activator homologous
                     sequence; transcription activator BRG1; ATP-dependent
                     helicase SMARCA4; protein BRG-1; protein brahma homolog 1;
                     BRG1-associated factor 190A"
                     /note="color: #993366"
                     /db_xref="{'GeneID': '6597', 'HGNC': 'HGNC:11100', 'MIM':
                     '603254'}"
                     /label="SMARCA4"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatctt ccactccaga cccacccctg
      781 ggcggaactc ctcggccagg tccttccccg ggccctggcc cttcccctgg agccatgctg
      841 ggccctagcc cgggtccctc gccgggctcc gcccacagca tgatggggcc cagcccaggg
      901 ccgccctcag caggacaccc catccccacc caggggcctg gagggtaccc tcaggacaac
      961 atgcaccaga tgcacaagcc catggagtcc atgcatgaga agggcatgtc ggacgacccg
     1021 cgctacaacc agatgaaagg aatggggatg cggtcagggg gccatgctgg gatggggccc
     1081 ccgcccagcc ccatggacca gcactcccaa ggttacccct cgcccctggg tggctctgag
     1141 catgcctcta gtccagttcc agccagtggc ccgtcttcgg ggccccagat gtcttccggg
     1201 ccaggaggtg ccccgctgga tggtgctgac ccccaggcct tggggcagca gaaccggggc
     1261 ccaaccccat ttaaccagaa ccagctgcac cagctcagag ctcagatcat ggcctacaag
     1321 atgctggcca gggggcagcc cctccccgac cacctgcaga tggcggtgca gggcaagcgg
     1381 ccgatgcccg ggatgcagca gcagatgcca acgctacctc caccctcggt gtccgcaaca
     1441 ggacccggcc ctggccctgg ccctggcccc ggcccgggtc ccggcccggc acctccaaat
     1501 tacagcaggc ctcatggtat gggagggccc aacatgcctc ccccaggacc ctcgggcgtg
     1561 ccccccggga tgccaggcca gcctcctgga gggcctccca agccctggcc tgaaggaccc
     1621 atggcgaatg ctgctgcccc cacgagcacc cctcagaagc tgattccccc gcagccaacg
     1681 ggccgccctt cccccgcgcc ccctgccgtc ccacccgccg cctcgcccgt gatgccaccg
     1741 cagacccagt cccccgggca gccggcccag cccgcgccca tggtgccact gcaccagaag
     1801 cagagccgca tcacccccat ccagaagccg cggggcctcg accctgtgga gatcctgcag
     1861 gagcgcgagt acaggctgca ggctcgcatc gcacaccgaa ttcaggaact tgaaaacctt
     1921 cccgggtccc tggccgggga tttgcgaacc aaagcgacca ttgagctcaa ggccctcagg
     1981 ctgctgaact tccagaggca gctgcgccag gaggtggtgg tgtgcatgcg gagggacaca
     2041 gcgctggaga cagccctcaa tgctaaggcc tacaagcgca gcaagcgcca gtccctgcgc
     2101 gaggcccgca tcactgagaa gctggagaag cagcagaaga tcgagcagga gcgcaagcgc
     2161 cggcagaagc accaggaata cctcaatagc attctccagc atgccaagga tttcaaggaa
     2221 tatcacagat ccgtcacagg caaaatccag aagctgacca aggcagtggc cacgtaccat
     2281 gccaacacgg agcgggagca gaagaaagag aacgagcgga tcgagaagga gcgcatgcgg
     2341 aggctcatgg ctgaagatga ggaggggtac cgcaagctca tcgaccagaa gaaggacaag
     2401 cgcctggcct acctcttgca gcagacagac gagtacgtgg ctaacctcac ggagctggtg
     2461 cggcagcaca aggctgccca ggtcgccaag gagaaaaaga agaaaaagaa aaagaagaag
     2521 gcagaaaatg cagaaggaca gacgcctgcc attgggccgg atggcgagcc tctggacgag
     2581 accagccaga tgagcgacct cccggtgaag gtgatccacg tggagagtgg gaagatcctc
     2641 acaggcacag atgcccccaa agccgggcag ctggaggcct ggctcgagat gaacccgggg
     2701 tatgaagtag ctccgaggtc tgatagtgaa gaaagtggct cagaagaaga ggaagaggag
     2761 gaggaggaag agcagccgca ggcagcacag cctcccaccc tgcccgtgga ggagaagaag
     2821 aagattccag atccagacag cgatgacgtc tctgaggtgg acgcgcggca catcattgag
     2881 aatgccaagc aagatgtcga tgatgaatat ggcgtgtccc aggcccttgc acgtggcctg
     2941 cagtcctact atgccgtggc ccatgctgtc actgagagag tggacaagca gtcagcgctt
     3001 atggtcaatg gtgtcctcaa acagtaccag atcaaaggtt tggagtggct ggtgtccctg
     3061 tacaacaaca acctgaacgg catcctggcc gacgagatgg gcctggggaa gaccatccag
     3121 accatcgcgc tcatcacgta cctcatggag cacaaacgca tcaatgggcc cttcctcatc
     3181 atcgtgcctc tctcaacgct gtccaactgg gcgtacgagt ttgacaagtg ggccccctcc
     3241 gtggtgaagg tgtcttacaa gggatcccca gcagcaagac gggcctttgt cccccagctc
     3301 cggagtggga agttcaacgt cttgctgacg acgtacgagt acatcatcaa agacaagcac
     3361 atcctcgcca agatccgttg gaagtacatg attgtggacg aaggtcaccg catgaagaac
     3421 caccactgca agctgacgca ggtgctcaac acgcactatg tggcaccccg ccgcctgctg
     3481 ctgacgggca caccgctgca gaacaagctt cccgagctct gggcgctgct caacttcctg
     3541 ctgcccacca tcttcaagag ctgcagcacc ttcgagcagt ggtttaacgc accctttgcc
     3601 atgaccgggg aaaaggtgga cctgaatgag gaggaaacca ttctcatcat ccggcgtctc
     3661 cacaaagtgc tgcggccctt cttgctccga cgactcaaga aggaagtcga ggcccagttg
     3721 cccgaaaagg tggagtacgt catcaagtgc gacatgtctg cgctgcagcg agtgctctac
     3781 cgccacatgc aggccaaggt aagtatcaag gttacaagac aggtttaagg agaccaataa
     3841 gctattattc gtataggata ctttatacga agttattaag ttatggagac cggtgtggca
     3901 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
     3961 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     4021 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
     4081 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     4141 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4201 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4261 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4321 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     4381 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4441 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg
     4501 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     4561 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     4621 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     4681 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     4741 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     4801 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     4861 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4921 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     4981 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5041 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5101 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5161 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5221 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5281 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5341 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     5401 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5461 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5521 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     5581 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     5641 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     5701 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     5761 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     5821 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     5881 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5941 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6001 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6061 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6121 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     6181 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6241 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6301 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6361 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6421 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6481 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6541 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6601 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
