LOCUS       .                       6774 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1493..3487
                     /translation="GVLLTDGSEKDKKGKGGTKTLMNTIMQLRKICNHPYMFQHIEESF
                     SEHLGFTGGIVQGLDLYRASGKFELLDRILPKLRATNHKVLLFCQMTSLMTIMEDYFAY
                     RGFKYLRLDGTTKAEDRGMLLKTFNEPGSEYFIFLLSTRAGGLGLNLQSADTVIIFDSD
                     WNPHQDLQAQDRAHRIGQQNEVRVLRLCTVNSVEEKILAAAKYKLNVDQKVIQAGMFDQ
                     KSSSHERRAFLQAILEHEEQDESRHCSTGSGSASFAHTAPPPAGVNPDLEEPPLKEEDE
                     VPDDETVNQMIARHEEEFDLFMRMDLDRRREEARNPKRKPRLMEEDELPSWIIKDDAEV
                     ERLTCEEEEEKMFGRGSRHRKEVDYSDSLTEKQWLKKITGKDIHDTASSVARGLQFQRG
                     LQFCTRASKAIEEGTLEEIEEEVRQKKSSRKRKRDSDAGSSTPTTSTRSRDKDDESKKQ
                     KKRGRPPAEKLSPNPPNLTKKMKKIVDAVIKYKDSSSGRQLSEVFIQLPSRKELPEYYE
                     LIRKPVDFKKIKERIRNHKYRSLNDLEKDVMLLCQNAQTFNLEGSLIYEDSIVLQSVFT
                     SVRQKIEKEDDSEGEESEEEEEGEEEGSESESRSVKVKIKLGRKEKAQDRLKGGRRRPS
                     RGSRAKPVVSDDDSEEEQEEDRSGSGSEED"
                     /note="isoform A is encoded by transcript variant 10;
                     sucrose nonfermenting-like 4; mitotic growth and
                     transcription activator; BRM/SWI2-related gene 1; homeotic
                     gene regulator; nuclear protein GRB1; brahma protein-like
                     1; SNF2-like 4; global transcription activator homologous
                     sequence; transcription activator BRG1; ATP-dependent
                     helicase SMARCA4; protein BRG-1; protein brahma homolog 1;
                     BRG1-associated factor 190A"
                     /note="color: #993366"
                     /db_xref="{'GeneID': '6597', 'HGNC': 'HGNC:11100', 'MIM':
                     '603254'}"
                     /gene="SMARCA4"
                     /product="transcription activator BRG1 isoform A"
                     /gene_synonym="BAF190; BAF190A; BRG1; CSS4; hSNF2b; MRD16;
                     RTPS2; SNF2; SNF2-beta; SNF2L4; SNF2LB; SWI2"
                     /transl_table=1
                     /codon_start=1
                     /protein_id="NP_001374212.1"
                     /label="SMARCA4"
     CDS             288..1318
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /product="site-specific recombinase"
                     /transl_table=1
                     /codon_start=1
                     /protein_id=""
                     /label="Cre"
     CDS             5094..5954
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /gene="bla"
                     /product="β-lactamase"
                     /transl_table=1
                     /codon_start=1
                     /label="AmpR"
     rep_origin      6125..6713
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     rep_origin      4252..4707
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     terminator      3563..3994
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   4037..4177
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     promoter        4989..5093
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     CDS             3494..3559
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /codon_start=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     protein_bind    1367..1400
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /label="lox2272-JTZ17"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac agggcgtgct
     1501 gctgactgat ggctccgaga aggacaagaa gggcaaaggc ggcaccaaga ccctgatgaa
     1561 caccatcatg cagctgcgga agatctgcaa ccacccctac atgttccagc acatcgagga
     1621 gtccttttcc gagcacttgg ggttcactgg cggcattgtc caagggctgg acctgtaccg
     1681 agcctcgggt aaatttgagc ttcttgatag aattcttccc aaactccgag caaccaacca
     1741 caaagtgctg ctgttctgcc aaatgacctc cctcatgacc atcatggaag attactttgc
     1801 gtatcgcggc tttaaatacc tcaggcttga tggaaccacg aaggcggagg accggggcat
     1861 gctgctgaaa accttcaacg agcccggctc tgagtacttc atcttcctgc tcagcacccg
     1921 ggctgggggg ctcggcctga acctccagtc ggcagacact gtgatcattt ttgacagcga
     1981 ctggaatcct caccaggacc tgcaagcgca ggaccgagcc caccgcatcg ggcagcagaa
     2041 cgaggtgcgt gtgctccgcc tctgcaccgt caacagcgtg gaggagaaga tcctagctgc
     2101 agccaagtac aagctcaacg tggaccagaa ggtgatccag gccggcatgt tcgaccagaa
     2161 gtcctccagc catgagcggc gcgccttcct gcaggccatc ctggagcacg aggagcagga
     2221 tgagagcaga cactgcagca cgggcagcgg cagtgccagc ttcgcccaca ctgcccctcc
     2281 gccagcgggc gtcaaccccg acttggagga gccacctcta aaggaggaag acgaggtgcc
     2341 cgacgacgag accgtcaacc agatgatcgc ccggcacgag gaggagtttg atctgttcat
     2401 gcgcatggac ctggaccgca ggcgcgagga ggcccgcaac cccaagcgga agccgcgcct
     2461 catggaggag gacgagctcc cctcgtggat catcaaggac gacgcggagg tggagcggct
     2521 gacctgtgag gaggaggagg agaagatgtt cggccgtggc tcccgccacc gcaaggaggt
     2581 ggactacagc gactcactga cggagaagca gtggctcaag aaaattacag gaaaagatat
     2641 ccatgacaca gccagcagtg tggcacgtgg gctacaattc cagcgtggcc ttcagttctg
     2701 cacacgtgcg tcaaaggcca tcgaggaggg cacgctggag gagatcgaag aggaggtccg
     2761 gcagaagaaa tcatcacgga agcgcaagcg agacagcgac gccggctcct ccaccccgac
     2821 caccagcacc cgcagccgcg acaaggacga cgagagcaag aagcagaaga agcgcgggcg
     2881 gccgcctgcc gagaaactct cccctaaccc acccaacctc accaagaaga tgaagaagat
     2941 tgtggatgcc gtgatcaagt acaaggacag cagcagtgga cgtcagctca gcgaggtctt
     3001 catccagctg ccctcgcgaa aggagctgcc cgagtactac gagctcatcc gcaagcccgt
     3061 ggacttcaag aagataaagg agcgcattcg caaccacaag taccgcagcc tcaacgacct
     3121 agagaaggac gtcatgctcc tgtgccagaa cgcacagacc ttcaacctgg agggctccct
     3181 gatctatgaa gactccatcg tcttgcagtc ggtcttcacc agcgtgcggc agaaaatcga
     3241 gaaggaggat gacagtgaag gcgaggagag tgaggaggag gaagagggcg aggaggaagg
     3301 ctccgaatcc gaatctcggt ccgtcaaagt gaagatcaag cttggccgga aggagaaggc
     3361 acaggaccgg ctgaagggcg gccggcggcg gccgagccga gggtcccgag ccaagccggt
     3421 cgtgagtgac gatgacagtg aggaggaaca agaggaggac cgctcaggaa gtggcagcga
     3481 agaagactgt acagactaca aagaccatga cggtgattat aaagatcatg acatcgacta
     3541 caaggatgac gatgacaagt aactcgagat aatcaacctc tggattacaa aatttgtgaa
     3601 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3661 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3721 tcctggttag ttcttgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     3781 ggggctcggc tgttgggcac tgacaattcc gtggtgttta tttgtgaaat ttgtgatgct
     3841 attgctttat ttgtaaccat ctagctttat ttgtgaaatt tgtgatgcta ttgctttatt
     3901 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     3961 tcaggttcag ggggagatgt gggaggtttt ttaaagatct ggagaccggt gtggcacgtg
     4021 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     4081 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     4141 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
     4201 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     4261 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     4321 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4381 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4441 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     4501 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4561 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt
     4621 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4681 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     4741 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     4801 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     4861 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     4921 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     4981 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5041 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5101 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5161 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5221 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5281 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     5341 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5401 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5461 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5521 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5581 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5641 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5701 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5761 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5821 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5881 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5941 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     6001 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6061 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6121 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6181 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6241 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     6301 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6361 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6421 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6481 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6541 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6601 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6661 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6721 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
