LOCUS       .                       6912 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             727..753
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /codon_start=1
                     /label="HA"
                     /note="color: #cc99b2"
     misc_feature    4097..4130
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     intron          4053..4093
                     /label="Splicing Donor"
                     /note="color: noColor"
     promoter        5127..5231
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   4175..4315
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     rep_origin      4390..4845
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      6263..6851
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             5232..6092
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /gene="bla"
                     /product="β-lactamase"
                     /transl_table=1
                     /codon_start=1
                     /label="AmpR"
     CDS             760..4052
                     /translation="DQEGGGDGQKAPSFQWRNYKLIVDPALDPALRRPSQKVYRYDGVH
                     FSVNDSKYIPVEDLQDPRCHVRSKNRDFSLPVPKFKLDEFYIGQIPLKEVTFARLNDNV
                     RETFLKDMCRKYGEVEEVEILLHPRTRKHLGLARVLFTSTRGAKETVKNLHLTSVMGNI
                     IHAQLDIKGQQRMKYYELIVNGSYTPQTVPTGGKALSEKFQGSGAATETAESRRRSSSD
                     TAAYPAGTTAVGTPGNGTPCSQDTSFSSSRQDTPSSFGQFTPQSSQGTPYTSRGSTPYS
                     QDSAYSSSTTSTSFKPRRSENSYQDAFSRRHFSASSASTTASTAIAATTAATASSSASS
                     SSLSSSSSSSSSSSSSQFRSSDANYPAYYESWNRYQRHTSYPPRRATREEPPGAPFAEN
                     TAERFPPSYTSYLPPEPSRPTDQDYRPPASEAPPPEPPEPGGGGGGGGPSPEREEVRTS
                     PRPASPARSGSPAPETTNESVPFAQHSSLDSRIEMLLKEQRSKFSFLASDTEEEEENSS
                     MVLGARDTGSEVPSGSGHGPCTPPPAPANFEDVAPTGSGEPGATRESPKANGQNQASPC
                     SSGDDMEISDDDRGGSPPPAPTPPQQPPPPPPPPPPPPPYLASLPLGYPPHQPAYLLPP
                     RPDGPPPPEYPPPPPPPPHIYDFVNSLELMDRLGAQWGGMPMSFQMQTQMLTRLHQLRQ
                     GKGLIAASAGPPGGAFGEAFLPFPPPQEAAYGLPYALYAQGQEGRGAYSREAYHLPMPM
                     AAEPLPSSSVSGEEARLPPREEAELAEGKTLPTAGTVGRVLAMLVQEMKSIMQRDLNRK
                     MVENVAFGAFDQWWESKEEKAKPFQNAAKQQAKEEDKEKTKLKEPGLLSLVDWAKSGGT
                     TGIEAFAFGSGLRGALRLPSFKVKRKEPSEISEASEEKRPRPSTPAEEDEDDPEQEKEA
                     GEPGRPGTKPPKRDEERGKTQGKHRKSFALDSEGEEASQESSSEKDEEDDEEDEEDEDR
                     EEAVDTTKKETEVSDGEDEESDSSSKCSLYADSDGENDSTSDSESSSSSSSSSSSSSSS
                     SSSSSSSSSESSSEDEEEEERPAALPSASPPPREVPVPTPAPVEVPVPE"
                     /note="hSET1A; lysine N-methyltransferase 2F; SET
                     domain-containing protein 1A; set1/Ash2 histone
                     methyltransferase complex subunit SET1"
                     /note="color: #993366"
                     /db_xref="{'GI': '55741677', 'CCDS': 'CCDS32435.1',
                     'GeneID': '9739', 'HGNC': 'HGNC:29010', 'MIM': '611052'}"
                     /EC_number="2.1.1.364"
                     /gene="SETD1A"
                     /product="histone-lysine N-methyltransferase SETD1A"
                     /gene_synonym="EPEDD; KMT2F; NEDSID; Set1; Set1A"
                     /transl_table=1
                     /codon_start=1
                     /protein_id="NP_055527.1"
                     /label="SETD1A"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatctg atcaggaagg tgggggagat
      781 gggcagaagg ccccgagctt ccagtggcgg aactacaagc tcatcgtgga tcctgccttg
      841 gaccctgccc tgcgcaggcc ttctcagaag gtgtaccgct atgatggagt ccacttcagt
      901 gtcaacgact caaagtatat accagtcgaa gacctccaag acccccgttg ccatgtcagg
      961 tccaaaaaca gagacttttc cctcccagtc cctaagttta agctggacga gttctatatt
     1021 ggacagattc cactgaagga agtgactttt gcaaggctga atgacaacgt gcgggagacc
     1081 ttcctgaagg atatgtgccg taagtacggt gaggtggaag aggtagagat cctccttcac
     1141 ccccgtacgc gcaagcacct gggcctggcc cgtgtgctct tcaccagcac tcggggcgcc
     1201 aaggaaacgg tcaaaaacct ccaccttacc tccgtcatgg gcaacatcat ccatgcccag
     1261 cttgacatca aaggacaaca acgaatgaaa tactatgaac taattgtcaa tggctcctac
     1321 acccctcaga ctgtgcccac tgggggcaag gccctgagtg agaagttcca aggctcgggt
     1381 gcagccactg agacggccga atcccgccgc cgctcttcct ctgacacagc tgcctaccca
     1441 gcaggcacca ctgcggtggg cactcctggc aacggcaccc cctgctccca ggacacaagc
     1501 ttctccagca gccgacaaga taccccatct tcctttggcc agttcacacc tcagtcctcc
     1561 caaggaaccc cctacacgtc tcggggcagc accccctact ctcaggactc tgcctactcc
     1621 agcagcacca cttcaacctc cttcaagccc cggcggtcag agaacagcta ccaagatgcc
     1681 ttttcccgcc gccacttctc tgcatcttca gcctccacaa ccgcctccac ggccatcgcc
     1741 gccaccactg cagccactgc ctcatcctcc gcctcttcct cctcattgtc ctcgtcctcc
     1801 tcgtcatcct cttcctcctc gtcctctcag tttcgtagtt ctgatgcaaa ctacccagcg
     1861 tattatgaaa gctggaatcg ctaccagcgc catacttcct acccaccacg ccgggccaca
     1921 cgggaggaac cccctggagc cccttttgct gaaaatacag ctgagcgctt cccaccttct
     1981 tacacctcct acctgccccc cgagcccagc cggcccaccg accaggacta ccggcctcct
     2041 gcctcagagg ctccaccccc ggagcctcca gaacctggtg gaggcggggg tggaggaggg
     2101 cccagccctg agagagaaga agttcggact tccccccgcc cagcctcccc tgcccgctct
     2161 ggctccccag ccccggagac caccaatgag agtgtgccct tcgcccagca cagcagcctg
     2221 gattcccgca tcgagatgct gctgaaggag cagcgctcca agttttcctt cttggcctct
     2281 gacacagagg aggaggaaga gaacagcagc atggtccttg gggccagaga tacagggagt
     2341 gaggtgcctt ctgggtcagg gcatgggccc tgcacacccc ctccggcccc agctaatttt
     2401 gaggatgtgg cacctacagg gagcggggag ccaggggcta cccgggagtc tcccaaggca
     2461 aatggacaga accaggcttc tccatgctct tctggagacg acatggagat ctccgacgac
     2521 gaccggggtg gctcaccccc tccggccccg acgccccctc agcagcctcc gccacctccc
     2581 cctcccccgc cgcctcctcc tccctacctg gcgtcccttc ctcttggtta tcctccccac
     2641 caacctgcct acctcctccc acccagacct gatgggccgc cgccccctga gtacccccca
     2701 cctcctccac cacccccgca catctatgac tttgtgaact ccttggagct catggaccga
     2761 cttggggctc agtggggagg gatgcccatg tccttccaga tgcagaccca gatgttaact
     2821 cggctccatc agctgcggca gggcaaggga ttgattgccg cctcagctgg cccccccggt
     2881 ggggcctttg gggaggcctt cctcccgttt ccacccccgc aggaggcagc ctacggcttg
     2941 ccgtatgctc tatatgcaca ggggcaggag ggcagagggg catactcacg ggaggcctac
     3001 cacctgccca tgccaatggc agccgagccc ctgccctcct cctcagtctc gggagaggag
     3061 gcccggctgc cacccaggga agaagcagag ctggcagagg gcaagaccct cccgacagca
     3121 ggcaccgtgg gccgtgtgct cgccatgctg gtccaggaga tgaagagcat catgcagcga
     3181 gacctcaacc gcaagatggt ggagaacgtg gccttcggag cctttgacca gtggtgggag
     3241 agcaaggagg agaaggccaa gccattccag aacgcggcca agcagcaagc caaggaggag
     3301 gataaagaga agacgaagct gaaggagcct ggcctgctgt ccctcgtgga ctgggccaag
     3361 agcgggggca ctacgggcat cgaggctttc gcctttgggt cagggctgag aggggccctg
     3421 cggctgcctt cattcaaggt aaagcggaaa gagccatcgg aaatttccga ggccagtgag
     3481 gaaaagaggc ctcgtccctc cactcctgct gaggaagatg aagacgaccc tgaacaagag
     3541 aaggaggctg gagagccagg acgtccgggg accaagcccc cgaagcggga cgaagagcga
     3601 ggcaagaccc agggcaagca ccgcaagtcc tttgctctgg acagcgaagg ggaggaggca
     3661 tcccaggagt cctcctcgga gaaggatgag gaggatgacg aggaagatga ggaagatgaa
     3721 gatcgagagg aagctgtgga taccacaaag aaggagacag aggtgtcgga tggcgaggac
     3781 gaggaaagcg attcgtcttc caaatgttct ctgtatgctg actcagatgg cgaaaatgac
     3841 agcacatcag actccgagag cagcagctct tccagctcct catcctcctc ctcctcctcg
     3901 tcctcatcct cctcgtcctc ttcatcctct gagtcctcct ctgaagatga agaggaagag
     3961 gagcggccag cagcccttcc ctcagcctcc ccgcccccca gagaagtccc agtgcccacg
     4021 ccagcacctg tggaggtgcc agtgccggaa aggtaagtat caaggttaca agacaggttt
     4081 aaggagacca ataagctatt attcgtatag gatactttat acgaagttat taagttatgg
     4141 agaccggtgt ggcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc
     4201 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
     4261 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc
     4321 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
     4381 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4441 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4501 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4561 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
     4621 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4681 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt
     4741 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     4801 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
     4861 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     4921 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     4981 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     5041 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     5101 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5161 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5221 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5281 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5341 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5401 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5461 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5521 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5581 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5641 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5701 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5761 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5821 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5881 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5941 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6001 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6061 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6121 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6181 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6241 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6301 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6361 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     6421 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6481 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6541 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6601 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6661 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6721 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6781 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6841 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6901 ttgctcacat gt
//
