LOCUS       .                       6604 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1493..3317
                     /codon_start=1
                     /product="histone-lysine N-methyltransferase SETD1A"
                     /translation="VAGSPVTPLPEQEASPARPAGPTEESPPSAPLRPPEPPAGPPAPA
                     PRPDERPSSPIPLLPPPKKRRKTVSFSAIEVVPAPEPPPATPPQAKFPGPASRKAPRGV
                     ERTIRNLPLDHASLVKSWPEEVSRGGRSRAGGRGRLTEEEEAEPGTEVDLAVLADLALT
                     PARRGLPALPAVEDSEATETSDEAERPRPLLSHILLEHNYALAVKPTPPAPALRPPEPV
                     PAPAALFSSPADEVLEAPEVVVAEAEEPKPQQLQQQREEGEEEGEEEGEEEEEESSDSS
                     SSSDGEGALRRRSLRSHARRRRPPPPPPPPPPRAYEPRSEFEQMTILYDIWNSGLDSED
                     MSYLRLTYERLLQQTSGADWLNDTHWVHHTITNLTTPKRKRRPQDGPREHQTGSARSEG
                     YYPISKKEKDKYLDVCPVSARQLEGVDTQGTNRVLSERRSEQRRLLSAIGTSAIMDSDL
                     LKLNQLKFRKKKLRFGRSRIHEWGLFAMEPIAADEMVIEYVGQNIRQMVADMREKRYVQ
                     EGIGSSYLFRVDHDTIIDATKCGNLARFINHCCTPNCYAKVITIESQKKIVIYSKQPIG
                     VDEEITYDYKFPLEDNKIPCLCGTESCRGSLN"
                     /gene_synonym="EPEDD; KMT2F; NEDSID; Set1; Set1A"
                     /protein_id="NP_055527.1"
                     /gene="SETD1A"
                     /transl_table=1
                     /EC_number="2.1.1.364"
                     /note="hSET1A; lysine N-methyltransferase 2F; SET
                     domain-containing protein 1A; set1/Ash2 histone
                     methyltransferase complex subunit SET1"
                     /note="color: #993366"
                     /db_xref="{'GI': '55741677', 'CCDS': 'CCDS32435.1',
                     'GeneID': '9739', 'HGNC': 'HGNC:29010', 'MIM': '611052'}"
                     /label="SETD1A"
     CDS             288..1318
                     /codon_start=1
                     /product="site-specific recombinase"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /protein_id=""
                     /transl_table=1
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /label="Cre"
     CDS             4924..5784
                     /codon_start=1
                     /product="β-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /transl_table=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /label="AmpR"
     rep_origin      5955..6543
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     rep_origin      4082..4537
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     terminator      3393..3824
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   3867..4007
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     promoter        4819..4923
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     CDS             3324..3389
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /transl_table=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     protein_bind    1367..1400
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /label="lox2272-JTZ17"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac agggttgcag
     1501 gctccccagt cacacccctg cccgaacagg aggcgtctcc agcaaggcct gcaggcccca
     1561 cggaggagtc accccccagt gcgcctctgc gtcccccaga accacctgct gggcccccgg
     1621 cccctgcccc acgccccgat gagcgtccct cttctcccat ccccctcctg cccccaccca
     1681 agaaacgccg gaaaactgtc tccttctctg ccatcgaggt ggtgccagcc ccggagcccc
     1741 ctccagccac accgccgcag gccaagtttc ccggcccagc ctcccgcaag gctccccggg
     1801 gcgtggagcg gaccatccgc aacctgcccc tggaccacgc atctctggtc aagagttggc
     1861 ccgaggaggt gtcccgagga ggccggagcc gggctggagg ccgaggccgc ctcaccgagg
     1921 aagaggaggc tgagccaggg acagaggtgg acctggcggt cctggccgac ctggccctga
     1981 cccctgcccg gcgcgggctg cctgccctgc ctgctgttga agactcagag gccacagaga
     2041 catcggacga ggccgagcgc cctaggcccc tgctcagcca catcctcctg gagcacaact
     2101 atgccctggc cgtcaagccc acgccccctg cgccagccct gcggcccccg gagccagtgc
     2161 ccgcacccgc cgccctcttc agttccccag ctgatgaggt cctggaggcc cccgaggtgg
     2221 tggtggctga ggcggaggag cccaagccgc agcaactgca gcagcagcgg gaggagggcg
     2281 aagaggaggg ggaggaagag ggggaggaag aggaggagga gtcctctgac agcagcagca
     2341 gcagcgatgg ggagggcgcc ctccggaggc gcagcctccg ctcccacgcc cggcgccgcc
     2401 gccctccgcc cccacccccg ccgccaccgc cccgcgccta cgagccacgc agtgagtttg
     2461 aacagatgac catcctgtat gacatttgga actcgggcct ggactcagag gacatgagtt
     2521 acctgcggct tacgtacgag cggctgctgc agcagacaag cggggctgac tggctcaacg
     2581 acactcactg ggtccatcac acaatcacca acctgaccac cccaaaacgc aagcggcggc
     2641 cccaggatgg gccccgggag caccagacag gctcagcccg cagcgaaggc tactacccca
     2701 tcagcaagaa ggagaaggac aagtacctgg acgtgtgccc agtctcggcc cggcagctgg
     2761 agggcgtgga cactcagggg acgaaccgcg tgctgtccga gcgccggtcc gagcagcggc
     2821 ggctgctgag cgccatcggt acctccgcca tcatggacag tgacctgctg aaactcaacc
     2881 agctcaagtt ccggaagaag aagctccgat ttggccggag ccggatccac gagtggggtc
     2941 tgtttgccat ggaacccatt gctgctgacg agatggtcat cgaatacgtg ggtcagaaca
     3001 tccgtcagat ggtggccgac atgcgggaga agcgctacgt gcaggagggc attggcagca
     3061 gctacctgtt ccgggtggac cacgacacca tcatcgatgc caccaagtgt ggcaacctgg
     3121 ccagattcat caaccactgc tgcacgccta actgctacgc caaggtcatc accatcgagt
     3181 cccagaagaa gatcgtgatc tactccaagc agcccattgg cgtggacgag gagatcacct
     3241 acgactacaa gttcccactg gaagacaaca agatcccgtg tctgtgtggc acagagagct
     3301 gccggggctc cctaaactgt acagactaca aagaccatga cggtgattat aaagatcatg
     3361 acatcgacta caaggatgac gatgacaagt aactcgagat aatcaacctc tggattacaa
     3421 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     3481 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     3541 cttgtataaa tcctggttag ttcttgccac ggcggaactc atcgccgcct gccttgcccg
     3601 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttta tttgtgaaat
     3661 ttgtgatgct attgctttat ttgtaaccat ctagctttat ttgtgaaatt tgtgatgcta
     3721 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     3781 attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagatct ggagaccggt
     3841 gtggcacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc
     3901 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt
     3961 tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg
     4021 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
     4081 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4141 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4201 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4261 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
     4321 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4381 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta
     4441 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4501 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc
     4561 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     4621 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     4681 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     4741 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     4801 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     4861 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     4921 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     4981 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5041 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     5101 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     5161 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5221 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5281 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5341 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5401 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5461 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5521 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5581 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5641 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5701 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5761 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     5821 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5881 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5941 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6001 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6061 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     6121 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6181 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6241 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6301 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6361 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6421 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6481 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6541 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6601 atgt
//
