LOCUS       .                       6999 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1493..3712
                     /codon_start=1
                     /note="isoform alpha-1 precursor is encoded by transcript
                     variant alpha-1; neurexin II"
                     /note="color: #993366"
                     /transl_table=1
                     /translation="GYIHYVFDLGNGPSLMKGNSDKPVNDNQWHNVVVSRDPGNVHTLK
                     IDSRTVTQHSNGARNLDLKGELYIGGLSKNMFSNLPKLVASRDGFQGCLASVDLNGRLP
                     DLIADALHRIGQVERGCDGPSTTCTEESCANQGVCLQQWDGFTCDCTMTSYGGPVCNDP
                     GTTYIFGKGGALITYTWPPNDRPSTRMDRLAVGFSTHQRSAVLVRVDSASGLGDYLQLH
                     IDQGTVGVIFNVGTDDITIDEPNAIVSDGKYHVVRFTRSGGNATLQVDSWPVNERYPAG
                     NFDNERLAIARQRIPYRLGRVVDEWLLDKGRQLTIFNSQAAIKIGGRDQGRPFQGQVSG
                     LYYNGLKVLALAAESDPNVRTEGHLRLVGEGPSVLLSAETTATTLLADMATTIMETTTT
                     MATTTTRRGRSPTLRDSTTQNTDDLLVASAECPSDDEDLEECEPSTGGELILPIITEDS
                     LDPPPVATRSPFVPPPPTFYPFLTGVGATQDTLPPPAARRPPSGGPCQAERDDSDCEEP
                     IEASGFASGEVFDSSLPPTDDEDFYTTFPLVTDRTTLLSPRKPAPRPNLRTDGATGAPG
                     VLFAPSAPAPNLPAGKMNHRDPLQPLLENPPLGPGAPTSFEPRRPPPLRPGVTSAPGFP
                     HLPTANPTGPGERGPPGAVEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEG
                     SYQVDQSRNYISNSAQSNGAVVKEKAPAAPKTPSKAKKNKDKEYYV"
                     /protein_id="NP_055895.1"
                     /db_xref="{'GI': '14211536', 'CCDS': 'CCDS8077.1',
                     'GeneID': '9379', 'HGNC': 'HGNC:8009', 'MIM': '600566'}"
                     /product="neurexin-2-beta isoform alpha-1 precursor"
                     /gene="NRXN2"
                     /label="NRXN2"
     CDS             5319..6179
                     /codon_start=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /product="β-lactamase"
                     /gene="bla"
                     /label="AmpR"
     rep_origin      6350..6938
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     rep_origin      4477..4932
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     terminator      3788..4219
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   4262..4402
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     promoter        5214..5318
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     CDS             3719..3784
                     /codon_start=1
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     CDS             288..1318
                     /codon_start=1
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /transl_table=1
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /protein_id=""
                     /product="site-specific recombinase"
                     /label="Cre"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     protein_bind    1367..1400
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /label="lox2272-JTZ17"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggggtacat
     1501 ccactacgtg tttgacctgg ggaatggccc gtccttgatg aaggggaact cagacaaacc
     1561 agtcaatgac aaccagtggc acaacgtggt ggtgtccagg gacccaggca acgtgcacac
     1621 gctcaagatt gactcccgca ctgtcacgca gcactccaat ggcgcccgaa acctcgatct
     1681 caaaggggag ttgtacattg gcggtctgag caagaatatg ttcagcaacc tgcccaagct
     1741 ggtggcctcc cgggatggct ttcagggctg cctggcctca gtggacctca acggacgtct
     1801 cccagacctc atcgccgacg ccctgcaccg cattgggcag gtggagaggg gctgtgatgg
     1861 ccccagcacc acctgcactg aagagtcctg tgccaaccag ggcgtctgct tgcagcagtg
     1921 ggatggcttc acctgcgact gcaccatgac ttcctatgga ggccctgtct gcaatgatcc
     1981 cgggaccaca tacatctttg ggaagggggg agcgctcatc acctacacgt ggccccccaa
     2041 tgacaggccc agcacgagga tggatcgcct ggccgtgggc ttcagcaccc accagcggag
     2101 cgctgtgctg gtgcgggtgg acagcgcctc cggccttgga gactacctgc agctgcacat
     2161 cgaccagggc accgtggggg tgatctttaa cgtgggcacg gacgacatta ccatcgacga
     2221 gcccaacgcc atagtaagcg acggcaaata ccacgtggtg cgcttcactc gaagcggcgg
     2281 caacgccacc ctgcaggtgg acagctggcc ggtcaacgag cggtacccgg caggaaactt
     2341 tgataacgag cgcctggcga ttgctagaca gagaatcccc taccggcttg gtcgagtagt
     2401 agatgagtgg ctgctcgaca aaggccgcca gctgaccatc ttcaacagcc aggctgccat
     2461 caagatcggg ggccgggatc agggccgccc cttccagggc caggtgtccg gcctctacta
     2521 caatgggctc aaggtgctgg cgctggccgc cgagagcgac cccaatgtgc ggactgaggg
     2581 tcacctgcgc ctggtggggg aggggccgtc cgtgctgctc agtgcggaga ccacggccac
     2641 caccctgctg gctgacatgg ccaccaccat catggagact accaccacca tggccactac
     2701 caccacgcgc cggggccgct cccccacact gagggacagc accacccaga acacagatga
     2761 cctgctggtg gcctctgctg agtgtccaag cgatgatgag gacctggagg agtgtgagcc
     2821 cagtactgga ggagagttaa tattgcccat tatcacggag gactccttag acccccctcc
     2881 cgtggccacc cgatccccct tcgtgccccc gccccctacc ttctacccct tcctcacggg
     2941 agtgggcgcc acccaagaca cgctgccccc gcccgccgcg cgccgcccgc cctctggggg
     3001 cccgtgccag gccgagcggg acgacagcga ctgcgaggag cccatcgagg cctcgggctt
     3061 cgcctccggg gaggtctttg actccagcct cccccccacg gacgacgagg acttttacac
     3121 cacctttccc ctggtcacgg accgcaccac cctcctgtca ccccgcaaac ccgctccccg
     3181 gcccaacctc aggacagatg gggccacggg cgcccctggg gtgctgtttg ccccctccgc
     3241 cccggccccc aacctgccgg cgggcaaaat gaaccaccga gacccgcttc agcccttgct
     3301 ggagaacccg cccttggggc ccggggcccc cacgtccttt gagccgcgga ggccccctcc
     3361 cctgcgcccc ggcgtgacct cagcccccgg cttcccccat ctgcccacag ccaaccccac
     3421 agggcctggg gagcggggcc cgccgggcgc agtggaggtg atccgggagt ccagcagcac
     3481 cacgggcatg gtggtgggca ttgtggcggc ggcggcgctc tgcatcctca tcctcctcta
     3541 cgccatgtat aagtaccgca atcgtgatga gggctcctac caggtggacc agagccgaaa
     3601 ctacatcagt aactcggccc agagcaatgg ggcggtggtg aaagagaagg ccccggctgc
     3661 ccccaagacg cccagcaagg ccaagaagaa caaagacaag gagtattatg tctgtacaga
     3721 ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga
     3781 caagtaactc gagataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     3841 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     3901 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttagttctt
     3961 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     4021 ggcactgaca attccgtggt gtttatttgt gaaatttgtg atgctattgc tttatttgta
     4081 accatctagc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     4141 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     4201 gatgtgggag gttttttaaa gatctggaga ccggtgtggc acgtgcggac cgagcggccg
     4261 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     4321 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     4381 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
     4441 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     4501 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4561 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4621 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4681 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4741 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4801 cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct
     4861 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     4921 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     4981 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     5041 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     5101 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5161 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5221 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5281 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5341 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5401 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5461 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5521 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5581 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5641 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5701 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5761 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5821 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5881 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5941 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6001 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6061 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6121 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6181 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6241 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6301 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6361 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6421 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6481 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6541 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6601 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6661 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6721 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6781 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6841 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6901 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6961 tacggttcct ggccttttgc tggccttttg ctcacatgt
//
