LOCUS       .                       6560 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             727..753
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /codon_start=1
                     /label="HA"
                     /note="color: #cc99b2"
     misc_feature    3745..3778
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     intron          3701..3741
                     /label="Splicing Donor"
                     /note="color: noColor"
     promoter        4775..4879
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   3823..3963
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     rep_origin      4038..4493
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      5911..6499
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             4880..5740
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /gene="bla"
                     /product="β-lactamase"
                     /transl_table=1
                     /codon_start=1
                     /label="AmpR"
     CDS             760..3700
                     /translation="MADAAASPVGKRLLLLFADTAASASASAPAAAAASGDPGPALRTR
                     AWRAGTVRAMSGAVPQDLAIFVEFDGCNWKQHSWVKVHAEEVIVLLLEGSLVWAPREDP
                     VLLQGIRVSIAQWPALTFTPLVDKLGLGSVVPVEYLLDRELRFLSDANGLHLFQMGTDS
                     QNQILLEHAALRETVNALISDQKLQEIFSRGPYSVQGHRVKIYQPEGEEGWLYGVVSHQ
                     DSITRLMEVSVTESGEIKSVDPRLIHVMLMDNSTPQSEGGTLKAVKSSKGKKKRESIEG
                     KDGRRRKSASDSGCDPASKKLKGDRGEVDSNGSDGGEASRGPWKGGNASGEPGLDQRAK
                     QPPSTFVPQINRNIRFATYTKENGRTLVVQDEPVGGDTPASFTPYSTATGQTPLAPEVG
                     GAENKEAGKTLEQVGQGIVASAAVVTTASSTPNTVRISDTGLAAGTVPEKQKGSRSQAS
                     GENSRNSILASSGFGAPLPSSSQPLTFGSGRSQSNGVLATENKPLGFSFGCSSAQEAQK
                     DTDLSKNLFFQCMSQTLPTSNYFTTVSESLADDSSSRDSFKQSLESLSSGLCKGRSVLG
                     TDTKPGSKAGSSVDRKVPAESMPTLTPAFPRSLLNARTPENHENLFLQPPKLSREEPSN
                     PFLAFVEKVEHSPFSSFASQASGSSSSATTVTSKVAPSWPESHSSADSASLAKKKPLFI
                     TTDSSKLVSGVLGSALTSGGPSLSAMGNGRSSSPTSSLTQPIEMPTLSSSPTEERPTVG
                     PGQQDNPLLKTFSNVFGRHSGGFLSSPADFSQENKAPFEAVKRFSLDERSLACRQDSDS
                     STNSDLSDLSDSEEQLQAKTGLKGIPEHLMGKLGPNGERSAELLLGKSKGKQAPKGRPR
                     TAPLKVGQSVLKDVSKVKKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQEQ
                     DDSTVACRFFHFRRLIFTRKGVLRVEGFLSPQQSDPDAMNLWIPSSSLAE"
                     /note="jumonji domain containing 1B; nuclear protein 5qNCA;
                     lysine (K)-specific demethylase 3B; jumonji
                     domain-containing protein 1B; jmjC domain-containing
                     histone demethylation protein 2B; [histone
                     H3]-dimethyl-L-lysine(9) demethylase 3B"
                     /note="color: #993366"
                     /db_xref="{'GI': '1519312227', 'CCDS': 'CCDS34242.1',
                     'GeneID': '51780', 'HGNC': 'HGNC:1337', 'MIM': '609373'}"
                     /EC_number="1.14.11.65"
                     /gene="KDM3B"
                     /product="lysine-specific demethylase 3B"
                     /gene_synonym="5qNCA; C5orf7; DIJOS; JMJD1B; NET22"
                     /transl_table=1
                     /codon_start=1
                     /protein_id="NP_057688.3"
                     /label="KDM3B"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatcta tggcggacgc ggcggcctcc
      781 ccggtgggca agcggctgct gctgctgttc gcggacactg cggcctcagc ctcggcctcg
      841 gctcccgcgg cggcagcggc gagcggagat ccggggcctg cgctgcgcac tcgagcctgg
      901 cgggccggca cggtgcgggc catgagcggg gcggtgcccc aggacctagc gatctttgta
      961 gaatttgatg gctgtaactg gaagcaacac tcctgggtaa aagttcatgc tgaggaagtt
     1021 attgtgcttc tgctggaagg gtctcttgta tgggcgcccc gtgaggaccc agtccttctc
     1081 cagggcattc gagtctccat tgcacaatgg ccagccctga cttttactcc ccttgtagat
     1141 aaactgggtt tgggttctgt ggttccagtg gaatatcttc tggatcgaga gcttcggttc
     1201 ctgtcagatg ccaatgggtt gcatctgttt cagatgggaa cagatagcca aaaccagatt
     1261 cttttggaac atgctgcact gagagaaaca gttaatgctt tgatcagtga ccaaaagcta
     1321 caagagatat tcagccgagg tccctacagt gttcaaggtc acagggtcaa aatatatcag
     1381 ccagaggggg aagaagggtg gctctatggt gttgtgagcc atcaggactc catcactcgt
     1441 cttatggagg tgtctgtaac tgagagtggt gagatcaagt cggtagatcc cagactaatc
     1501 catgtgatgc tgatggataa ttcaacgcct caaagcgagg ggggtacgtt aaaagcagta
     1561 aaatcttcca aaggaaagaa gaagagagaa agcatagagg ggaaagatgg ccggaggagg
     1621 aaaagtgctt cggactctgg gtgtgaccct gcatcaaaga aattaaaagg agacaggggt
     1681 gaagtagaca gtaatgggag cgatggaggt gaggcaagcc gagggccctg gaaaggaggg
     1741 aatgccagtg gagagccagg gctggatcag agagccaagc agccaccgtc tacatttgtc
     1801 ccccagataa accgcaacat tcgctttgcc acttacacca aagaaaacgg caggactctg
     1861 gtggtgcagg atgagcctgt aggtggggac acacctgcat ctttcactcc atattctaca
     1921 gccacaggtc agacaccttt ggccccagag gtgggtggag ccgaaaacaa agaggcagga
     1981 aaaacactgg aacaagttgg ccagggcata gtggcttccg cagctgtggt cactaccgcc
     2041 agctccaccc caaacacagt gaggatctca gacactggcc ttgcagcagg gactgtgcca
     2101 gaaaaacaga aaggcagccg gtcgcaggcc tcgggagaga attcaagaaa ttctattctg
     2161 gcctcttctg gatttggagc acctctccct agttcatcgc aacctttgac ttttggaagt
     2221 ggaaggagcc agtccaatgg tgttctagcc acagagaaca aacctttggg cttctctttt
     2281 ggctgtagct ctgcacaaga ggcacagaaa gacactgatc tctccaaaaa cttgtttttt
     2341 caatgcatgt cccaaacttt acctaccagt aactacttca ctactgtttc agagagtttg
     2401 gctgatgatt cttctagtcg ggactcattc aaacaaagcc ttgagagcct gagctcaggc
     2461 ctgtgtaaag gcagatccgt tcttggaaca gacactaagc caggctctaa ggctggcagc
     2521 tctgtggacc ggaaagtgcc tgcagagtcc atgcccaccc tcactccagc cttcccacgg
     2581 agcctcctaa atgcccgtac cccagagaat catgaaaatc tatttttaca gccccccaaa
     2641 ttgtcccgag aagagccttc taatcctttc ctggcatttg tggagaaagt tgaacacagc
     2701 cctttcagta gttttgcatc tcaggcatca ggtagctcct cttctgctac cactgtcacc
     2761 tccaaggtgg cacccagctg gcccgagtct cactcctctg cagattcggc atctttagca
     2821 aagaagaaac ccctcttcat tacaactgac tcctccaagc tagtatctgg tgttctgggc
     2881 tcagctctta ccagtggggg cccaagcctc tctgccatgg ggaatggccg ctccagctcg
     2941 cccaccagca gcctcactca gcccattgag atgccaactc tctcctctag ccccacagag
     3001 gagaggccaa ctgtggggcc tgggcagcag gacaatcccc tcctcaaaac ctttagtaac
     3061 gtctttggca ggcactcagg cggctttctg tcctccccgg cagatttttc acaggagaac
     3121 aaagctcctt ttgaagctgt gaaaaggttc tcactggatg aacgaagctt ggcttgcaga
     3181 caagactcgg actccagcac caacagtgac ctgtcagatt tgagtgactc tgaggagcag
     3241 ctgcaggcta agacaggcct gaagggaatt ccagagcacc tgatggggaa gctgggcccc
     3301 aatggggagc gcagtgctga gctgttgctg ggcaaaagca aagggaagca ggcccccaag
     3361 ggccggcctc ggactgcccc cctgaaagtt ggccagtcag tgctgaaaga tgtaagcaaa
     3421 gtgaagaagc tgaagcaatc tggagagccc ttcctgcagg atgggtcatg catcaatgtg
     3481 gcacctcatc tgcacaagtg tcgtgaatgc cgcctggagc ggtaccggaa gtttaaggaa
     3541 caggagcaag atgattctac tgtagcctgc cgtttctttc acttccggag gttgatcttc
     3601 actcgaaaag gggtgctccg tgtggagggg tttttaagcc cccagcaaag tgaccctgat
     3661 gccatgaacc tgtggattcc ctcttcctcc ctagcagaag gtaagtatca aggttacaag
     3721 acaggtttaa ggagaccaat aagctattat tcgtatagga tactttatac gaagttatta
     3781 agttatggag accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg
     3841 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     3901 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     3961 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     4021 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4081 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     4141 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4201 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     4261 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     4321 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg
     4381 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     4441 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     4501 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     4561 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     4621 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     4681 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4741 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4801 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4861 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4921 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4981 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5041 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5101 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5161 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5221 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5281 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5341 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5401 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5461 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5521 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5581 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5641 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5701 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5761 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5821 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5881 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5941 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6001 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     6061 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6121 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6181 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6241 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6301 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6361 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6421 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6481 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6541 ctggcctttt gctcacatgt
//
