LOCUS       .                       7121 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    1367..1400
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /label="lox2272-JTZ17"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     CDS             3841..3906
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /transl_table=1
                     /codon_start=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     promoter        5336..5440
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     repeat_region   4384..4524
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     terminator      3910..4341
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     rep_origin      4599..5054
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     rep_origin      6472..7060
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             5441..6301
                     /product="β-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /transl_table=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /codon_start=1
                     /label="AmpR"
     CDS             288..1318
                     /product="site-specific recombinase"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /transl_table=1
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /protein_id=""
                     /codon_start=1
                     /label="Cre"
     CDS             1493..3834
                     /product="lysine-specific demethylase 3B"
                     /translation="IDLETSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRG
                     VREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKSRPRSETEEMGDEEVFSWLKCAKG
                     QSHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCISRQNKSVLRPAVTNGMS
                     QLPSINPSASSGNETTFSGGGGPAPVTTPEPDHVPKADSTDIRSEEPLKTDSSASNSNS
                     ELKAIRPPCPDTAPPSSALHWLADLATQKAKEETKEAGSLRSVLNKESHSPFGLDSFNS
                     TAKVSPLTPKLFNSLLLGPTASNNKTEGSSLRDLLHSGPGKLPQTPLDTGIPFPPVFST
                     SSAGVKSKASLPNFLDHIIASVVENKKTSDASKRACNLTDTQKEVKEMVMGLNVLDPHT
                     SHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQPVLVSGVHKKLKSELWKPEAFSQEFG
                     DQDVDLVNCRNCAIISDVKVRDFWDGFEIICKRLRSEDGQPMVLKLKDWPPGEDFRDMM
                     PTRFEDLMENLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTT
                     NLHLDVSDAVNVMVYVGIPIGEGAHDEEVLKTIDEGDADEVTKQRIHDGKEKPGALWHI
                     YAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRKRLYEEYGVQGWAIVQ
                     FLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKL
                     QVKNIIYHAVKDAVGTLKAHESKLARS"
                     /EC_number="1.14.11.65"
                     /gene="KDM3B"
                     /transl_table=1
                     /note="jumonji domain containing 1B; nuclear protein 5qNCA;
                     lysine (K)-specific demethylase 3B; jumonji
                     domain-containing protein 1B; jmjC domain-containing
                     histone demethylation protein 2B; [histone
                     H3]-dimethyl-L-lysine(9) demethylase 3B"
                     /note="color: #993366"
                     /protein_id="NP_057688.3"
                     /codon_start=1
                     /db_xref="{'GI': '1519312227', 'CCDS': 'CCDS34242.1',
                     'GeneID': '51780', 'HGNC': 'HGNC:1337', 'MIM': '609373'}"
                     /gene_synonym="5qNCA; C5orf7; DIJOS; JMJD1B; NET22"
                     /label="KDM3B"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac agggatagat
     1501 ctagagacct caaaatacat cctggccaat gttggggacc agttctgcca gctcgtaatg
     1561 tctgagaagg aggccatgat gatggtggag ccacaccaga aagtggcatg gaagcgagct
     1621 gtgcgtggtg tacgggagat gtgtgatgtg tgtgaaacaa ctctcttcaa catccactgg
     1681 gtttgtcgca aatgtggatt tggggtctgc cttgactgtt accggctcag gaaaagccgg
     1741 ccacgcagtg agacagaaga gatgggtgat gaagaagttt tctcctggtt gaagtgtgca
     1801 aagggacagt cccacgaacc agagaatctc atgcccacac aaattattcc tggcacagct
     1861 ctttacaata ttggagacat ggtacatgct gcccggggca agtggggaat taaagcaaac
     1921 tgcccttgta tcagtcgaca gaacaaatct gtattgagac ctgccgtcac caatgggatg
     1981 tcacagcttc ctagcataaa ccctagtgcc tcttctggaa acgaaactac cttctctggc
     2041 ggaggaggac cggcaccagt aacaactcca gagccggacc atgttcccaa agccgacagc
     2101 actgacatca gatctgaaga gcctctgaaa acagacagtt cggcatcaaa tagcaatagt
     2161 gaactgaaag ccatcaggcc tccttgccct gacacggccc caccctcctc cgccctgcac
     2221 tggttggcag atttagcaac tcagaaggct aaagaagaaa caaaagaagc agggtccctg
     2281 aggtcggtgc tcaataaaga gtctcattca ccctttgggc tggactcgtt caactccact
     2341 gcaaaggtct ctccgctgac tccaaagctt tttaacagtc tgttgctggg tcccactgcc
     2401 tccaacaaca aaaccgaagg gtctagcctt cgagacctcc ttcactccgg gccgggaaaa
     2461 cttcctcaaa cccccttgga cacaggcata ccctttcccc cggtcttctc tacatcctca
     2521 gcaggagtga agagcaaggc cagcctaccc aactttcttg accacatcat tgcctcagtg
     2581 gtagaaaata agaaaacctc agatgcttca aagcgggcct gcaacttgac tgatacccag
     2641 aaggaagtga aggagatggt gatggggtta aatgtgctag atccccatac ttctcactcc
     2701 tggctttgtg atgggaggct tctgtgtctc catgacccca gcaacaaaaa caattggaag
     2761 atcttccggg agtgttggaa gcaaggtcag ccagtgctgg tttcgggggt acataaaaag
     2821 ctcaagtctg agctctggaa gccagaagcc tttagccagg aatttggaga ccaggatgta
     2881 gacttggtga actgcaggaa ctgtgctata atttccgatg tgaaagttcg ggatttctgg
     2941 gatggtttcg agatcatatg caaacgacta cggtcagaag atgggcagcc aatggtgctc
     3001 aaactcaagg actggcctcc tggggaagat tttcgagaca tgatgccaac caggtttgaa
     3061 gatctgatgg agaaccttcc tctgccagaa tataccaaac gagatggcag gctcaatctg
     3121 gcctctaggc tacctagcta ctttgtaagg cctgatctgg gccccaagat gtacaacgcc
     3181 tatgggttga taacagcaga agatagaaga gttggtacaa caaatcttca cttagatgtg
     3241 tctgatgctg ttaatgtgat ggtgtatgtt gggattccca tcggggaggg tgctcatgat
     3301 gaagaggtac tcaagacaat tgacgaggga gatgccgatg aggtgacgaa gcagaggatt
     3361 catgatggaa aagagaagcc aggtgcttta tggcacatct atgcagccaa ggatgcagag
     3421 aagatccggg agctgctccg aaaggttgga gaagaacaag gccaagagaa cccccctgat
     3481 catgacccaa ttcatgacca aagttggtac ctggaccaga ccctccgtaa gcgactctat
     3541 gaggagtatg gcgtgcaagg ctgggctatt gtgcagttcc taggtgatgc tgttttcata
     3601 cctgctggag ccccacacca ggttcacaat ctatacagtt gcataaaagt agcagaagac
     3661 tttgtatctc cagaacatgt aaagcactgt ttccgcctga ctcaggaatt caggcatctc
     3721 tctaacactc atacaaatca tgaggataaa ctgcaggtga agaacatcat ttaccatgca
     3781 gtgaaagatg cggttggcac cctcaaggct catgaatcca aactggcaag gtcctgtaca
     3841 gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat
     3901 gacaagtaac tcgagataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     3961 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4021 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttagttc
     4081 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     4141 tgggcactga caattccgtg gtgtttattt gtgaaatttg tgatgctatt gctttatttg
     4201 taaccatcta gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     4261 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     4321 gagatgtggg aggtttttta aagatctgga gaccggtgtg gcacgtgcgg accgagcggc
     4381 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     4441 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     4501 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     4561 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     4621 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4681 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4741 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4801 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4861 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4921 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc
     4981 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     5041 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     5101 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     5161 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     5221 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     5281 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5341 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5401 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5461 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5521 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5581 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5641 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     5701 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5761 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5821 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5881 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5941 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     6001 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6061 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6121 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6181 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6241 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6301 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6361 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6421 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6481 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6541 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6601 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6661 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6721 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6781 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6841 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6901 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6961 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7021 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7081 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
