LOCUS       .                       6834 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             727..753
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
                     /transl_table=1
                     /codon_start=1
                     /label="HA"
                     /note="color: #cc99b2"
     misc_feature    4019..4052
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     intron          3975..4015
                     /label="Splicing Donor"
                     /note="color: noColor"
     promoter        5049..5153
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   4097..4237
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     rep_origin      4312..4767
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      6185..6773
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             5154..6014
                     /product="β-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /transl_table=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /codon_start=1
                     /label="AmpR"
     CDS             760..3974
                     /product="PH-interacting protein"
                     /translation="SCERKGLSELRSELYFLIARFLEDGPCQQAAQVLIREVAEKELLP
                     RRTDWTGKEHPRTYQNLVKYYRHLAPDHLLQICHRLGPLLEQEIPQSVPGVQTLLGAGR
                     QSLLRTNKSCKHVVWKGSALAALHCGRPPESPVNYGSPPSIADTLFSRKLNGKYRLERL
                     VPTAVYQHMKMHKRILGHLSSVYCVTFDRTGRRIFTGSDDCLVKIWATDDGRLLATLRG
                     HAAEISDMAVNYENTMIAAGSCDKMIRVWCLRTCAPLAVLQGHSASITSLQFSPLCSGS
                     KRYLSSTGADGTICFWLWDAGTLKINPRPAKFTERPRPGVQMICSSFSAGGMFLATGST
                     DHIIRVYFFGSGQPEKISELEFHTDKVDSIQFSNTSNRFVSGSRDGTARIWQFKRREWK
                     SILLDMATRPAGQNLQGIEDKITKMKVTMVAWDRHDNTVITAVNNMTLKVWNSYTGQLI
                     HVLMGHEDEVFVLEPHPFDPRVLFSAGHDGNVIVWDLARGVKIRSYFNMIEGQGHGAVF
                     DCKCSPDGQHFACTDSHGHLLIFGFGSSSKYDKIADQMFFHSDYRPLIRDANNFVLDEQ
                     TQQAPHLMPPPFLVDVDGNPHPSRYQRLVPGRENCREEQLIPQMGVTSSGLNQVLSQQA
                     NQEISPLDSMIQRLQQEQDLRRSGEAVISNTSRLSRGSISSTSEVHSPPNVGLRRSGQI
                     EGVRQMHSNAPRSEIATERDLVAWSRRVVVPELSAGVASRQEEWRTAKGEEEIKTYRSE
                     EKRKHLTVPKENKIPTVSKNHAHEHFLDLGESKKQQTNQHNYRTRSALEETPRPSEEIE
                     NGSSSSDEGEVVAVSGGTSEEEERAWHSDGSSSDYSSDYSDWTADAGINLQPPKKVPKN
                     KTKKAESSSDEEEESEKQKQKQIKKEKKKVNEEKDGPISPKKKKPKERKQKRLAVGELT
                     ENGLTLEEWLPSTWITDTIPRRCPFVPQMGDEVYYFRQGHEAYVEMARKNKIYSINPKK
                     QPWHKMELREQELMKIVGIKYEVGLPTLCCLKLAFLDPDTGKLTGGSFTMKYHDMPDVI
                     DFLVLRQQFDDAKYRRWNIGDRF"
                     /gene="PHIP"
                     /transl_table=1
                     /note="DDB1 and CUL4 associated factor 14; IRS-1 PH
                     domain-binding protein; WD repeat-containing protein 11"
                     /note="color: #993366"
                     /protein_id="NP_060404.4"
                     /db_xref="{'GI': '1081684143', 'CCDS': 'CCDS4987.1',
                     'GeneID': '55023', 'HGNC': 'HGNC:15673', 'MIM': '612870'}"
                     /codon_start=1
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; WDR11"
                     /label="PHIP"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatctt cttgtgagag gaaaggcctc
      781 tcggagctgc gatcggagct ctacttcctc atcgcccggt tcctggaaga tggaccctgt
      841 cagcaggcgg ctcaggtgct gatccgcgag gtggccgaga aggagctgct gccccggcgc
      901 accgactgga ccgggaagga gcatcccagg acctaccaga atctggtgaa gtattacaga
      961 cacttagcac ctgatcactt gctgcaaata tgtcatcgac taggacctct tcttgaacaa
     1021 gaaattcctc aaagtgttcc tggagtacaa actttattag gagctggaag acagtcttta
     1081 ctacgcacaa ataaaagctg caagcatgtt gtgtggaaag gatctgctct ggctgcgttg
     1141 cactgtggaa gaccacctga gtcaccagtt aactatggta gcccacccag cattgcggat
     1201 actctgtttt caaggaagct gaatgggaaa tacagacttg agcgacttgt tccaactgca
     1261 gtgtatcagc acatgaaaat gcataaacga attcttggac acttgtcatc tgtgtactgt
     1321 gtaacttttg atcgaactgg cagacggata tttactggtt ctgatgactg tcttgtgaaa
     1381 atatgggcaa cagatgatgg gaggttgtta gctaccttaa gaggacatgc tgctgaaata
     1441 tcagacatgg ctgtaaacta tgagaatacc atgatagcag ctggaagttg tgataaaatg
     1501 atccgagtct ggtgtcttcg aacctgtgca cctttggctg ttcttcaggg ccatagtgca
     1561 tctattacat cactacagtt ctcaccattg tgcagtggct caaagagata tctatcttct
     1621 actggggcag atggcactat ttgtttttgg ctctgggatg ctggaaccct taaaataaac
     1681 ccaagacctg caaaatttac agagcgccct cggcctggag ttcaaatgat ctgttcttct
     1741 tttagtgctg gtggaatgtt tctggcgacg ggaagcacag atcatattat tcgggtttat
     1801 ttttttggat caggtcagcc agagaaaata tcagaattgg agtttcatac tgacaaagtt
     1861 gacagtatcc agttttccaa cactagtaac aggtttgtaa gtggcagtcg tgatgggaca
     1921 gcacgtattt ggcaatttaa acgaagagag tggaagagca ttttgttgga tatggctact
     1981 cgtccagcag gccaaaacct tcaaggaata gaagataaaa tcacaaaaat gaaggttact
     2041 atggtagctt gggatcgaca tgacaataca gttataactg cagttaataa catgactctg
     2101 aaagtttgga attcttacac tggtcaacta attcatgtcc tgatgggtca tgaagatgag
     2161 gtatttgttc ttgaaccaca cccgttcgat cctagagttc tcttttctgc tggtcatgat
     2221 ggaaacgtga tagtgtggga tctggcaaga ggagtcaaaa tacgatctta tttcaatatg
     2281 attgaaggcc aaggacatgg cgcagtattt gactgcaaat gctctcctga tggtcagcat
     2341 tttgcatgca cagactctca tggacatctt ttaatttttg gctttgggtc cagtagcaaa
     2401 tatgacaaga tagcagatca gatgttcttt catagtgatt atcggccact tattcgtgat
     2461 gccaacaatt ttgtattaga tgaacagact cagcaagcac ctcatcttat gcctccccct
     2521 tttttggttg atgttgatgg taaccctcat ccatcaagat atcaaagatt agttcctggc
     2581 cgtgaaaatt gcagggagga gcaactcatc cctcagatgg gagtaacttc ctcaggactg
     2641 aatcaagttt taagtcagca agcaaaccag gagatcagcc cactggacag catgattcaa
     2701 agactacaac aggagcaaga cctgagacgt tctggtgaag cagttatcag taataccagc
     2761 cgtttaagta gaggctccat aagttctacc tcagaggttc attcaccacc aaacgtagga
     2821 ctaagacgta gtggacaaat tgaaggtgta cggcaaatgc acagcaacgc accaagaagt
     2881 gaaatagcca cagagcggga tctggtagct tggagtcgaa gggtggtagt acccgagcta
     2941 tcagctggtg tagccagtag gcaagaagaa tggagaactg caaagggaga agaagaaata
     3001 aagacttaca ggtcagaaga gaaaagaaaa cacttaactg ttccaaaaga gaataaaata
     3061 cccactgtct caaagaatca tgctcatgag catttcctgg atcttggaga atccaaaaag
     3121 caacagacaa atcaacacaa ttatcgtaca agatctgcat tggaagagac tcctagaccc
     3181 tcagaagaga tagaaaatgg cagtagttct tcagatgaag gcgaagtagt tgctgtcagt
     3241 ggtggaacat ccgaagaaga agagagagca tggcacagtg atggcagttc tagtgactac
     3301 tccagtgatt actctgactg gacagcagat gcaggaatta atctgcagcc accaaagaaa
     3361 gttcctaaga ataaaaccaa gaaagcagaa agcagttcag atgaagaaga agaatctgaa
     3421 aaacagaagc aaaaacagat taaaaaggaa aagaaaaaag taaatgaaga aaaagatgga
     3481 ccaatatcac caaagaaaaa gaagcccaaa gaaagaaaac aaaagagatt ggctgtggga
     3541 gaactaactg aaaatggttt gacattagaa gaatggttgc catcaacatg gattacagat
     3601 accattcccc gaagatgtcc atttgtgcca cagatgggtg atgaggttta ttatttccga
     3661 caaggacatg aagcctatgt cgaaatggcc cggaaaaata aaatatatag tatcaatccc
     3721 aaaaaacaac catggcataa aatggagcta cgggaacaag aacttatgaa aatagttggc
     3781 ataaagtatg aagtgggatt acctaccctt tgctgcctta aacttgcttt tctagatcct
     3841 gatactggta aactgactgg cggatcattt accatgaaat accatgatat gcctgacgtt
     3901 atagatttcc tagtcttgag acaacaattt gatgatgcaa aatacaggcg atggaatata
     3961 ggtgaccgct tcaggtaagt atcaaggtta caagacaggt ttaaggagac caataagcta
     4021 ttattcgtat aggatacttt atacgaagtt attaagttat ggagaccggt gtggcacgtg
     4081 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     4141 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     4201 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
     4261 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     4321 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     4381 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4441 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4501 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     4561 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4621 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt
     4681 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4741 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     4801 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     4861 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     4921 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     4981 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     5041 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5101 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5161 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5221 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5281 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5341 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     5401 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5461 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5521 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5581 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5641 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5701 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5761 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5821 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5881 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5941 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     6001 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     6061 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6121 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6181 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6241 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6301 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     6361 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6421 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6481 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6541 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6601 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6661 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6721 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6781 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
