LOCUS       .                       6766 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             727..753
                     /codon_start=1
                     /translation="YPYDVPDYA"
                     /transl_table=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label="HA"
                     /note="color: #cc99b2"
     misc_feature    3951..3984
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     intron          3907..3947
                     /label="Splicing Donor"
                     /note="color: noColor"
     promoter        4981..5085
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   4029..4169
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     rep_origin      4244..4699
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      6117..6705
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             5086..5946
                     /codon_start=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /transl_table=1
                     /product="β-lactamase"
                     /gene="bla"
                     /label="AmpR"
     CDS             760..3906
                     /codon_start=1
                     /note="isoform 1 is encoded by transcript variant 1;
                     cortactin SH3 domain-binding protein; GKAP/SAPAP
                     interacting protein; proline-rich synapse associated
                     protein 1; cortactin-binding protein 1"
                     /note="color: #993366"
                     /protein_id="NP_036441.2"
                     /translation="MPRSPTSSEDEMAQSFSDYSVGSESDSSKEETIYDTIRATAEKPG
                     GARTEESQGNTLVIRVVIHDLQQTKCIRFNPDATVWVAKQRILCTLTQSLKDVLNYGLF
                     QPASNGRDGKFLDEERLLREYPQPVGEGVPSLEFRYKKRVYKQASLDEKQLAKLHTKTN
                     LKKCMDHIQHRLVEKITKMLDRGLDPNFHDPETGETPLTLAAQLDDSVEVIKALKNGGA
                     HLDFRAKDGMTALHKAARARNQVALKTLLELGASPDYKDSYGLTPLYHTAIVGGDPYCC
                     ELLLHEHATVCCKDENGWHEIHQACRYGHVQHLEHLLFYGADMSAQNASGNTALHICAL
                     YNQDSCARVLLFRGGNKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDIVPFREAPA
                     YSNRRRRPPNTLAAPRVLLRSNSDNNLNASAPDWAVCSTATSHRSLSPQLLQQMPSKPE
                     GAAKTIGSYVPGPRSRSPSLNRLGGAGEDGKRPQPLWHVGSPFALGANKDSLSAFEYPG
                     PKRKLYSAVPGRLFVAVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFP
                     AECVEEVQCKPRDSQAETRADRSKKLFRHYTVGSYDSFDTSSDCIIEEKTVVLQKKDNE
                     GFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVV
                     KVGHRQVVNMIRQGGNHLVLKVVTVTRNLDPDDTARKKAPPPPKRAPTTALTLRSKSMT
                     SELEELVDKASVRKKKDKPEEIVPASKPSRAAENMAVEPRVATIKQRPSSRCFPAGSDM
                     NSVYERQGIAVMTPTVPGSPKAPFLGIPRGTMRRQKSIDSRIFLSGITEEERQFLAPPM
                     LKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTTYNCPKSPTPRVYGTIKPAFNQNSAA
                     KVSPATRSDTVATMMREKGMYFRRELDRYSLDSEDLYSRNAGPQANFRNKRGQMPENPY
                     SEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSS
                     Q"
                     /transl_table=1
                     /db_xref="{'GI': '226817313', 'GeneID': '22941', 'HGNC':
                     'HGNC:14295', 'MIM': '603290'}"
                     /product="SH3 and multiple ankyrin repeat domains protein 2
                     isoform 1"
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /label="SHANK2"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatcta tgccgcgcag cccaacatcc
      781 agcgaggacg agatggccca gagcttctcc gactactccg tggggtcgga gtcagacagc
      841 tccaaagaag agaccatcta tgacacgatc cgggccactg cggagaagcc gggcggtgcc
      901 aggacggagg agagccaggg caacacgctg gtgatccgcg tggtcatcca tgacctgcag
      961 cagacgaaat gcattcgatt taacccggat gccacagtgt gggttgcaaa gcagcggatc
     1021 ctgtgtacat taacccagag tttgaaagat gtcctgaact acggcctgtt ccagccggcc
     1081 agcaatgggc gtgacggcaa gttcctggat gaggagcggc tcctgcgcga gtacccacag
     1141 cccgtgggtg agggcgttcc ttccctggag tttcgataca agaagcgggt gtataaacaa
     1201 gccagtctcg atgagaaaca gttggccaag ctccacacga agaccaatct gaagaaatgc
     1261 atggatcaca ttcagcatcg cttggtggag aagatcacca agatgctgga ccgaggcctg
     1321 gatcccaatt tccacgaccc ggagaccgga gagacccccc tgaccttagc cgctcagctg
     1381 gacgactctg tggaggtcat caaagctctc aaaaatggtg gagctcacct ggacttccgt
     1441 gccaaagatg ggatgaccgc cctacacaaa gctgcccgag cgaggaacca agttgccctg
     1501 aagacccttt tagagcttgg tgcatcccca gattataaag acagttacgg cctcaccccg
     1561 ctgtatcaca cagccatcgt cggaggtgat ccctactgct gcgagcttct cctgcacgaa
     1621 cacgccactg tgtgctgcaa agatgagaac ggctggcacg agatccacca ggcctgcagg
     1681 tacgggcacg tgcagcacct ggagcacctg ctgttctacg gggcagacat gagtgcccag
     1741 aatgcctcgg ggaacacggc cttgcacatc tgcgccctct acaaccagga cagctgtgca
     1801 agagtgcttc tgtttcgagg cggaaataag gagttaaaaa actacaacag ccagactcca
     1861 tttcaggtgg ccataatagc aggcaacttt gagctggcag aatacatcaa gaaccacaag
     1921 gaaacagaca ttgtgccctt ccgagaggcc ccggcgtact ccaaccgccg gcggcggccc
     1981 cccaacacgc tggccgcccc ccgggtcctg ctgcgctcca acagtgacaa caacctcaat
     2041 gccagcgctc ccgactgggc cgtctgctcc acggccacct cgcaccgcag cctgtcaccc
     2101 cagctgctgc agcagatgcc cagcaagccc gagggggccg cgaagaccat tgggagctac
     2161 gtgcccgggc cccgcagccg gtccccatcg ctcaacaggc tgggcggcgc aggcgaggac
     2221 ggcaagaggc cgcagcctct ctggcatgtc gggtcgcctt ttgctcttgg tgccaacaag
     2281 gactcactct cggccttcga gtacccgggg cccaagcgga agctctacag tgccgtgccc
     2341 gggaggctct tcgtcgctgt caagccatac caaccccaag tggacggcga gatccccctt
     2401 caccgcggtg acagggtcaa agttctgagc atcggtgaag ggggcttctg ggaaggcagc
     2461 gcccgcggcc acatcggatg gtttccggcg gagtgcgtgg aggaggtcca gtgtaagccc
     2521 agggacagcc aggcagaaac ccgcgctgac cgcagcaaga agcttttcag gcactacacc
     2581 gtgggctcct atgacagctt cgacacttcc agtgactgca ttattgagga gaagacggtg
     2641 gtcctgcaga aaaaagacaa tgagggcttt ggattcgtgc ttcgaggggc caaagctgac
     2701 acacccattg aagaattcac accaacaccg gctttcccag ccctacagta cctggagtcc
     2761 gtggatgaag gtggggtggc gtggcaagcc ggactaagga ccggggactt cttgattgag
     2821 gttaacaatg agaatgttgt caaagtcggc cacaggcagg tggtgaacat gatccggcag
     2881 ggagggaatc acctggtcct taaggtggtc acggtgacca ggaatctgga ccccgacgac
     2941 accgccagga agaaagctcc cccgcctcca aagcgggcac cgaccacagc cctcaccctg
     3001 cgctccaagt ccatgacctc ggagctggag gagctcgtgg ataaagcctc ggtccggaag
     3061 aagaaggata aacccgagga gatagtcccg gcctccaagc cctcccgcgc tgctgagaac
     3121 atggctgtgg aaccgagggt ggcgaccatc aagcagcggc ccagcagccg gtgcttcccg
     3181 gcgggctcag acatgaactc tgtgtacgaa cgccaaggaa tcgccgtgat gacgcccact
     3241 gttcctggga gcccaaaagc cccgtttctg ggcatccctc gaggtacgat gcgaaggcag
     3301 aaatcaatag acagcagaat ctttctatca ggaataacag aggaagagcg gcagtttctg
     3361 gctcctccaa tgctgaagtt caccagaagc ctgtccatgc cggacacctc tgaggacatc
     3421 ccccctccac cgcagtctgt gcccccgtcc ccaccaccac cttccccaac cacttacaac
     3481 tgccccaagt ccccaactcc aagagtctac gggacgatta agcctgcgtt caatcagaat
     3541 tctgccgcca aggtgtcccc cgccaccagg tccgacaccg tggccaccat gatgagggag
     3601 aaggggatgt acttcaggag agagctggac cgctactcct tggactctga agacctctac
     3661 agtcggaatg ccggcccgca agccaacttc cgcaacaaga gaggccagat gccagaaaac
     3721 ccatactcag aggtggggaa gatcgccagc aaagccgtct acgtccccgc caagcccgcc
     3781 aggcggaagg ggatgctggt gaagcagtcc aacgtggagg acagccccga gaagacgtgc
     3841 tccatcccta tcccgaccat catcgtgaag gagccgtcca ccagcagcag cggcaagagc
     3901 agccaggtaa gtatcaaggt tacaagacag gtttaaggag accaataagc tattattcgt
     3961 ataggatact ttatacgaag ttattaagtt atggagaccg gtgtggcacg tgcggaccga
     4021 gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
     4081 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
     4141 gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca
     4201 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     4261 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4321 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4381 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4441 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4501 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4561 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     4621 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     4681 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     4741 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     4801 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     4861 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     4921 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     4981 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5041 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5101 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5161 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5221 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5281 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5341 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5401 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5461 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5521 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5581 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5641 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5701 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5761 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     5821 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     5881 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     5941 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6001 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6061 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6121 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6181 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6241 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     6301 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6361 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6421 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6481 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6541 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6601 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6661 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6721 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//
