LOCUS       .                       6933 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    1367..1400
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /bound_moiety="Cre recombinase"
                     /label="lox2272-JTZ17"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     CDS             3653..3718
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /codon_start=1
                     /transl_table=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     promoter        5148..5252
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     repeat_region   4196..4336
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     terminator      3722..4153
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     rep_origin      4411..4866
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     rep_origin      6284..6872
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     CDS             5253..6113
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /gene="bla"
                     /product="β-lactamase"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     CDS             288..1318
                     /protein_id=""
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /product="site-specific recombinase"
                     /codon_start=1
                     /transl_table=1
                     /label="Cre"
     CDS             1493..3646
                     /protein_id="NP_109590.3"
                     /translation="VSSSPQKEGRVSQRARVPKPGAGSKLSDRPLHALKRKSAFMAPVP
                     TKKRNLVLRSRSSSSSNASGNGGDGKEERPEGSPTLFKRMSSPKKAKPTKGNGEPATKL
                     PPPETPDACLKLASRAAFQGAMKTKVLPPRKGRGLKLEAIVQKITSPSLKKFACKAPGA
                     SPGNPLSPSLSDKDRGLKGAGGSPVGVEEGLVNVGTGQKLPTSGADPLCRNPTNRSLKG
                     KLMNSKKLSSTDCFKTEAFTSPEALQPGGTALAPKKRSRKGRAGAHGLSKGPLEKRPYL
                     GPALLLTPRDRASGTQGASEDNSGGGGKKPKMEELGLASQPPEGRPCQPQTRAQKQPGH
                     TNYSSYSKRKRLTRGRAKNTTSSPCKGRAKRRRQQQVLPLDPAEPEIRLKYISSCKRLR
                     SDSRTPAFSPFVRVEKRDAFTTICTVVNSPGDAPKPHRKPSSSASSSSSSSSFSLDAAG
                     ASLATLPGGSILQPRPSLPLSSTMHLGPVVSKALSTSCLVCCLCQNPANFKDLGDLCGP
                     YYPEHCLPKKKPKLKEKVRPEGTCEEASLPLERTLKGPECAAAATAGKPPRPDGPADPA
                     KQGPLRTSARGLSRRLQSCYCCDGREDGGEEAAPADKGRKHECSKEAPAEPGGEAQEHW
                     VHEACAVWTGGVYLVAGKLFGLQEAMKVAVDMMCSSCQEAGATIGCCHKGCLHTYHYPC
                     ASDAGCIFIEENFSLKCPKHKRLP"
                     /note="Smith-Magenis syndrome chromosome region"
                     /note="color: #993366"
                     /gene="RAI1"
                     /db_xref="{'CCDS': 'CCDS11188.1', 'GeneID': '10743',
                     'HGNC': 'HGNC:9834', 'MIM': '607642'}"
                     /product="retinoic acid-induced protein 1"
                     /codon_start=1
                     /gene_synonym="SMCR; SMS"
                     /transl_table=1
                     /label="RAI1"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtctccag
     1501 cagcccccag aaggagggca gggtgagcca gcgggcaagg gtccccaaac ctggtgcagg
     1561 cagcaagctc tctgaccggc ccctccatgc gctcaaaagg aagtcggcct tcatggcgcc
     1621 ggtccccacc aagaagcgga acctggtctt gcggagccgc agcagcagca gcagcaacgc
     1681 cagtggcaat gggggagatg ggaaggagga gaggcctgag ggttccccca ccctcttcaa
     1741 gaggatgtct tctcccaaga aagccaagcc caccaagggc aatggcgagc ctgccacaaa
     1801 gctcccaccc ccggagaccc ccgatgcctg cctcaagctc gcctctcggg cagccttcca
     1861 gggggccatg aagaccaagg tgctgccacc ccggaagggc cggggcctga agctggaagc
     1921 catcgtgcag aagatcacct cgcccagcct caagaagttc gcatgtaaag cgccaggggc
     1981 ctctcctggt aatcctctga gcccatccct ttccgacaaa gaccgtgggc tcaagggtgc
     2041 tgggggcagc ccagtggggg tggaagaagg cctggtaaat gtgggcaccg ggcagaagct
     2101 cccaacttct ggggctgatc cgttatgcag aaatccaacc aacagatcct taaaaggcaa
     2161 actcatgaac agtaagaaac tgtcttctac tgactgtttc aaaaccgagg ccttcacatc
     2221 cccggaggcc ctgcagcctg gggggactgc cctggcgcct aagaagagga gccggaaagg
     2281 ccgggcaggg gcccatggac tctccaaagg cccgctggag aagcggccct atcttggccc
     2341 ggctctgctc ctgactcccc gagacagggc cagtggcaca caaggggcca gtgaggacaa
     2401 ctctggtgga ggaggcaaga agccaaagat ggaggagctg ggcctggcct cccagccccc
     2461 ggagggcagg ccctgccagc cccagacaag ggcacagaaa cagccaggcc acaccaacta
     2521 cagcagctat tccaagcgga agcgcctcac tcggggccgg gccaagaaca ccacctcttc
     2581 accctgtaag gggcgtgcca agcgacgacg acagcagcag gtgctgcccc tggatcccgc
     2641 agagcctgaa atccgcctca agtacatttc ctcttgcaag cggctgaggt cagacagccg
     2701 gacccccgcc ttctcaccct tcgtgcgggt ggagaagcga gacgcgttca ccaccatatg
     2761 cactgttgtc aactcccctg gagatgcgcc caagccccac aggaagcctt cctcctctgc
     2821 ctcctcttcc tcatcctcgt cctcgttctc cttggatgca gccggggcct ccctggccac
     2881 actccctgga ggctccatcc tgcagccgcg gccctccttg cccctctcct ccacgatgca
     2941 cttggggcct gtggtttcca aggccctgag tacctcttgc cttgtttgct gcctctgcca
     3001 aaacccggcc aacttcaagg accttgggga cctctgtggg ccctactacc ctgaacactg
     3061 cctccccaaa aagaagccaa aactcaagga gaaggtgcgg ccagaaggca cctgtgagga
     3121 ggcctcgctg ccgcttgaga gaacactcaa aggtcccgag tgtgcagctg ccgccactgc
     3181 cgggaagccc cccaggcctg acggcccagc tgacccggcc aagcagggcc cactgcgcac
     3241 cagtgcccgg ggcctgtccc ggaggctgca gagctgctac tgctgtgatg gccgggagga
     3301 tgggggcgag gaggcagccc cagccgacaa gggtcgcaaa catgagtgca gcaaggaggc
     3361 tccggcagag cccggcgggg aggcccagga gcactgggtg catgaggcct gtgccgtgtg
     3421 gaccggcggc gtctacctgg tggccgggaa gctctttggg ctgcaggagg ccatgaaggt
     3481 ggccgtggac atgatgtgtt ccagctgcca agaagccggg gccaccatcg ggtgctgcca
     3541 caaaggatgc ctccacacct accactaccc gtgtgccagc gatgcaggtt gcatattcat
     3601 cgaagagaac ttttctttga aatgtcccaa acataagagg ctgccgtgta cagactacaa
     3661 agaccatgac ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta
     3721 actcgagata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     3781 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3841 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttagt tcttgccacg
     3901 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     3961 gacaattccg tggtgtttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatc
     4021 tagctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     4081 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
     4141 ggaggttttt taaagatctg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa
     4201 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     4261 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     4321 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     4381 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
     4441 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4501 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4561 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4621 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4681 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4741 accctatctc gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4801 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     4861 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     4921 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     4981 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     5041 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     5101 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5161 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5221 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     5281 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     5341 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5401 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     5461 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5521 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5581 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5641 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5701 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5761 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     5821 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     5881 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5941 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6001 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6061 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6121 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6181 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6241 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6301 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6361 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6421 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6481 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6541 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6601 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6661 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6721 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6781 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6841 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6901 tcctggcctt ttgctggcct tttgctcaca tgt
//
