LOCUS       .                       7248 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             727..753
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
                     /transl_table=1
                     /codon_start=1
                     /label="HA"
                     /note="color: #cc99b2"
     misc_feature    4433..4466
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     intron          4389..4429
                     /label="Splicing Donor"
                     /note="color: noColor"
     promoter        5463..5567
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   4511..4651
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     rep_origin      4726..5181
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      6599..7187
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             5568..6428
                     /product="β-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /transl_table=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /codon_start=1
                     /label="AmpR"
     CDS             760..4388
                     /product="transcription factor 20 isoform 1"
                     /translation="QSFREQSSYHGNQQSYPQEVHGSSRLEEFSPRQAQMFQNFGGTGG
                     SSGSSGSGSGGGRRGAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVTTGTPQ
                     PPQRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSGLGGVSHYQQDYTGPFSPGS
                     AQYQQQASSQQQQQQVQQLRQQLYQSHQPLPQATGQPASSSSHLQPMQRPSTLPSSAAG
                     YQLRVGQFGQHYQSSASSSSSSSFPSPQRFSQSGQSYDGSYNVNAGSQYEGHNVGSNAQ
                     AYGTQSNYSYQPQSMKNFEQAKIPQGTQQGQQQQQPQQQQHPSQHVMQYTNAATKLPLQ
                     SQVGQYNQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLMQTGENLQCGQ
                     GSVPMGSRNRILQLMPQLSPTPSMMPSPNSHAAGFKGFGLEGVPEKRLTDPGLSSLSAL
                     STQVANLPNTVQHMLLSDALTPQKKTSKRPSSSKKADSCTNSEGSSQPEEQLKSPMAES
                     LDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEPPRLNASPAA
                     REEATSPGAKDMPLSSDGNPKVNEKTVGVIVSREAMTGRVEKPGGQDKGSQEDDPAATQ
                     RPPSNGGAKETSHASLPQPEPPGGGGSKGNKNGDNNSNHNGEGNGQSGHSAAGPGFTSR
                     TEPSKSPGSLRYSYKDSFGSAVPRNVSGFPQYPTGQEKGDFTGHGERKGRNEKFPSLLQ
                     EVLQGYHHHPDRRYSRSTQEHQGMAGSLEGTTRPNVLVSQTNELASRGLLNKSIGSLLE
                     NPHWGPWERKSSSTAPEMKQINLTDYPIPRKFEIEPQSSAHEPGGSLSERRSVICDISP
                     LRQIVRDPGAHSLGHMSADTRIGRNDRLNPTLSQSVILPGGLVSMETKLKSQSGQIKEE
                     DFEQSKSQASFNNKKSGDHCHPPSIKHESYRGNASPGAATHDSLSDYGPQDSRPTPMRR
                     VPGRVGGREGMRGRSPSQYHDFAEKLKMSPGRSRGPGGDPHHMNPHMTFSERANRSSLH
                     TPFSPNSETLASAYHANTRAHAYGDPNAGLNSQLHYKRQMYQQQPEEYKDWSSGSAQGV
                     IAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPVGTYHDPSAQEAGRCLMSS
                     DGLPNKGMELKHGSQKLQESCWDLSRQTSPAKSSGPPGMSSQK"
                     /gene="TCF20"
                     /transl_table=1
                     /note="isoform 1 is encoded by transcript variant 3;
                     stromelysin-1 platelet-derived growth factor-responsive
                     element binding protein; SPRE-binding protein; nuclear
                     factor SPBP; stromelysin-1 PDGF-responsive element-binding
                     protein"
                     /note="color: #993366"
                     /protein_id="NP_001365347.1"
                     /db_xref="{'GI': '1808532973', 'CCDS': 'CCDS14033.1',
                     'GeneID': '6942', 'HGNC': 'HGNC:11631', 'MIM': '603107'}"
                     /codon_start=1
                     /gene_synonym="AR1; DDVIBA; SPBP; TCF-20"
                     /label="TCF20"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatctc agtcctttcg ggagcaaagc
      781 agttaccacg gaaaccagca aagctaccca caggaggtac acggctcatc ccggctagaa
      841 gagttcagcc ctcgtcaggc ccagatgttc cagaattttg gaggtacagg tggcagtagt
      901 ggcagcagtg gcagtggcag tggtggtgga cgacgaggag cagcagctgc tgcggcagcg
      961 atggctagcg agacctctgg ccatcaaggt taccagggtt tcaggaaaga ggctggagat
     1021 ttttactaca tggcaggcaa caaagacccc gtgactacag gaaccccaca gcctcctcag
     1081 cgaaggcctt ctgggcctgt gcagagctat ggaccccccc aggggagcag ctttggcaat
     1141 cagtatggga gtgagggtca tgtgggccag tttcaagcac agcactctgg ccttggcggt
     1201 gtgtcacatt atcagcagga ttacactggg cctttctctc cagggagtgc tcagtaccaa
     1261 cagcaggctt ccagccagca gcagcagcag caagtccagc agttgagaca acagctttac
     1321 cagtcccatc agcccctgcc acaggccact ggccaaccag catccagctc atcccatcta
     1381 cagccaatgc agcggccctc aactctgcca tcctctgctg ctggttacca gttaagagtg
     1441 ggtcagtttg gccaacacta tcagtcttct gcttcctcct cctcctcctc ctccttccct
     1501 tcaccacagc gttttagcca gtctggacag agctatgatg gcagttacaa tgtgaatgct
     1561 ggatctcagt atgaaggaca caatgtgggt tctaatgcac aggcttatgg aacacaatcc
     1621 aattacagct atcagcctca atctatgaag aattttgaac aggcaaagat tccacaaggg
     1681 acccaacagg ggcagcagca gcagcaaccg cagcaacaac aacacccttc tcagcatgtg
     1741 atgcagtata ctaacgctgc caccaagctg cccctgcaaa gccaagtggg gcagtacaac
     1801 cagcctgagg ttcctgtgag gtcccccatg cagtttcacc agaacttcag ccccatttct
     1861 aacccttctc cagctgcctc tgtggttcag tctccaagct gtagttctac cccatctcct
     1921 ctcatgcaga ctggggagaa tctccagtgt gggcaaggca gtgtgcctat gggttccaga
     1981 aacagaattt tacagttaat gcctcaactc agtccaaccc catcaatgat gcccagtcct
     2041 aattctcatg ctgcaggctt caaagggttt ggactagaag gggtaccaga aaagcgactg
     2101 acagatcctg ggttgagtag tttgagtgct ctgagtactc aagtggccaa tcttcctaac
     2161 actgtccagc acatgttact ttctgatgcc ctgactcctc agaagaagac ctccaagagg
     2221 ccctcatctt ccaagaaagc agatagctgc acaaattctg aaggctcctc acaacctgaa
     2281 gaacagctga agtcccctat ggcagagtca ttagatggag gctgctccag cagttcagag
     2341 gatcaaggcg agagagtgcg gcaactaagt ggccagagca ccagctctga caccacctac
     2401 aagggtggag cctctgagaa agctggctcc tcaccggcac aaggtgctca gaatgaaccc
     2461 cccagactca atgctagtcc tgccgcaaga gaagaggcca cctcaccagg cgctaaggac
     2521 atgccattgt catccgacgg gaacccaaag gttaatgaga agactgttgg ggtgattgtc
     2581 tcccgggaag ccatgacagg tcgggtagaa aagcctggtg gacaagataa aggctcccaa
     2641 gaggatgatc ctgcagccac tcaaaggcca cctagcaatg gtggggcaaa ggaaaccagt
     2701 catgcatcac ttccccagcc agagcctcca ggaggaggag ggagcaaagg aaacaagaat
     2761 ggcgataaca actccaacca taatggagaa ggaaatggcc agagtggcca ctctgcagcg
     2821 ggccctggtt ttacgagcag aactgagcct agcaaatctc ctggaagtct gcgctatagt
     2881 tacaaagata gtttcgggtc agccgtgcca cgaaatgtca gtggctttcc tcagtatcct
     2941 acagggcaag aaaagggaga tttcactggc catggggaac gaaagggtag aaatgaaaaa
     3001 ttcccaagcc tcctgcagga agtgcttcag ggttaccacc accaccctga caggagatat
     3061 tctaggagta ctcaagagca tcaggggatg gctggtagcc tagaaggaac cacaaggccc
     3121 aatgtcttgg ttagtcaaac caatgaatta gctagcaggg gccttctgaa caaaagcatt
     3181 gggtctctat tagaaaatcc ccactggggc ccctgggaaa ggaaatcaag cagcacagct
     3241 cctgaaatga aacagatcaa tttgactgac tatccaattc ccagaaagtt tgaaatagag
     3301 cctcagtcat cagcacatga gcctgggggt tccctctctg aaagaagatc agtgatctgt
     3361 gatatttctc cactaagaca gattgtcagg gacccagggg ctcactcact gggacacatg
     3421 agtgccgaca ccagaattgg gaggaatgac cgtctcaatc caactttaag tcagtcggtc
     3481 attcttcctg gtggtttggt gtccatggaa accaagctga aatcccagag cgggcagata
     3541 aaagaggaag actttgaaca gtctaaatct caagctagtt tcaacaacaa gaaatctgga
     3601 gaccactgcc atcctcctag catcaagcat gagtcttacc gcggcaatgc cagccctgga
     3661 gcagcaaccc atgattccct ttcagactat ggcccgcaag acagcagacc cacgccaatg
     3721 cggcgggtcc ctggcagagt tggtggtcgg gagggcatga ggggtcggtc cccttctcaa
     3781 tatcatgact ttgcagaaaa attgaaaatg tctcctgggc ggagcagagg tccaggggga
     3841 gaccctcatc acatgaatcc acacatgacc ttttcagaga gggctaaccg gagttcttta
     3901 cacactccct tttctcccaa ctcagaaacc ctggcctctg cttatcatgc aaatactcgg
     3961 gctcatgctt atggggaccc taacgcaggt ttgaattctc agctgcatta taagagacag
     4021 atgtaccaac agcaaccaga ggagtataaa gactggagca gcggttctgc tcagggagta
     4081 attgctgcag cacagcacag gcaggagggg ccacggaaga gtccaaggca gcagcagttt
     4141 cttgacagag tacggagccc tctgaaaaat gacaaagatg gtatgatgta tggcccacca
     4201 gtggggactt accatgaccc cagtgcccag gaggctgggc gctgcctaat gtctagtgat
     4261 ggtctgccta acaagggcat ggaattaaag catggctccc agaagttaca agaatcctgt
     4321 tgggatcttt ctcggcaaac ttctccagcc aaaagcagcg gtcctccagg aatgtccagt
     4381 caaaaaaggt aagtatcaag gttacaagac aggtttaagg agaccaataa gctattattc
     4441 gtataggata ctttatacga agttattaag ttatggagac cggtgtggca cgtgcggacc
     4501 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     4561 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     4621 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
     4681 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
     4741 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     4801 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4861 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4921 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     4981 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     5041 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga
     5101 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     5161 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
     5221 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     5281 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     5341 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     5401 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     5461 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5521 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5581 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5641 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5701 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5761 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5821 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5881 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5941 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6001 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6061 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6121 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6181 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6241 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6301 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6361 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6421 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6481 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6541 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6601 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6661 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6721 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     6781 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6841 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6901 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6961 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7021 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7081 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7141 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7201 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
