LOCUS       .                       7039 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    1367..1400
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /label="lox2272-JTZ17"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     CDS             3747..3812
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /transl_table=1
                     /codon_start=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     promoter        5254..5358
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     repeat_region   4302..4442
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     terminator      3828..4259
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     rep_origin      4517..4972
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     rep_origin      6390..6978
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     CDS             5359..6219
                     /gene="bla"
                     /product="β-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /transl_table=1
                     /codon_start=1
                     /label="AmpR"
     CDS             288..1318
                     /product="site-specific recombinase"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /transl_table=1
                     /protein_id=""
                     /codon_start=1
                     /label="Cre"
     CDS             1493..3740
                     /gene="TCF20"
                     /product="transcription factor 20 isoform 1"
                     /translation="YGPPHETDGHGLAEATQSSKPGSVMLRLPGQEDHSSQNPLIMRRR
                     VRSFISPIPSKRQSQDVKNSSTEDKGRLLHSSKEGADKAFNSYAHLSHSQDIKSIPKRD
                     SSKDLPSPDSRNCPAVTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAE
                     AGGDTVTLDDILSLKSGPPEGGSVAVQDADIEKRKGEVASDLVSPANQELHVEKPLPRS
                     SEEWRGSVDDKVKTETHAETVTAGKEPPGAMTSTTSQKPGSNQGRPDGSLGGTAPLIFP
                     DSKNVPPVGILAPEANPKAEEKENDTVTISPKQEGFPPKGYFPSGKKKGRPIGSVNKQK
                     KQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAVPIVEPQEP
                     EIKLKYATQPLDKTDAKNKSFYPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRS
                     LTPPPSSTESKALPASSFMLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQD
                     YAATLPKNPPPKRATEMQSKVKVRHKSASNGSKTDTEEEEEQQQQQKEQRSLAAHPRFK
                     RRHRSEDCGGGPRSLSRGLPCKKAATEGSSEKTVLDSKPSVPTTSEGGPELELQIPELP
                     LDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMKCSHCQEAGATLGCYNKGCS
                     FRYHYPCAIDADCLLHEENFSVRCPKHKPPLPCPLPPLQNKTAKGSLSTEQSERG"
                     /note="isoform 1 is encoded by transcript variant 3;
                     stromelysin-1 platelet-derived growth factor-responsive
                     element binding protein; SPRE-binding protein; nuclear
                     factor SPBP; stromelysin-1 PDGF-responsive element-binding
                     protein"
                     /note="color: #993366"
                     /db_xref="{'GI': '1808532973', 'CCDS': 'CCDS14033.1',
                     'GeneID': '6942', 'HGNC': 'HGNC:11631', 'MIM': '603107'}"
                     /gene_synonym="AR1; DDVIBA; SPBP; TCF-20"
                     /transl_table=1
                     /protein_id="NP_001365347.1"
                     /codon_start=1
                     /label="TCF20"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtatgggc
     1501 cgccccatga gactgatgga catggactag ctgaggctac acagtcatcc aaacctggta
     1561 gtgttatgct gagacttcca ggccaggagg atcattcttc tcaaaacccc ttaatcatga
     1621 ggaggcgtgt tcgttctttt atctctccca ttcccagtaa gagacagtca caagatgtaa
     1681 agaacagtag cactgaagat aaaggtcgcc tccttcactc atcaaaagaa ggcgctgata
     1741 aagcattcaa ttcctatgcc catctttctc acagtcagga tatcaagtct atccctaaga
     1801 gagattcctc caaggacctt ccaagtccag atagtagaaa ctgccctgct gttaccctca
     1861 caagccctgc taagaccaaa atactgcccc cacggaaagg acggggattg aaattggaag
     1921 ctatagttca gaagattaca tccccaaata ttaggaggag cgcatcttcg aacagtgcgg
     1981 aggctggggg agacacggtt acgcttgatg atatactgtc tttgaagagt ggtcctcctg
     2041 aaggtgggag tgttgctgtt caggatgctg acatagagaa gagaaaaggt gaggtggctt
     2101 cggacctagt cagtccagca aaccaggagt tgcacgtaga gaaacctctt ccaaggtctt
     2161 cagaagagtg gcgtggcagc gtggatgaca aagtgaagac agagacacat gcagaaacag
     2221 ttactgccgg aaaggaaccc cctggtgcca tgacatccac aacctcacag aagcctggta
     2281 gtaaccaagg gagaccagat ggttccctgg gtggaacagc acctttaatc tttccagact
     2341 caaagaatgt acctccagtg ggcatattgg cccctgaggc aaaccccaag gctgaagaga
     2401 aggagaacga tacagtgacg atttcaccga agcaagaggg tttccctcca aagggatatt
     2461 tcccatcagg aaagaagaag gggagaccca ttggtagtgt gaataagcaa aagaaacagc
     2521 agcagccacc gcctccaccc cctcagcccc cacagatacc agaaggttct gcagatggag
     2581 agccaaagcc aaaaaaacag aggcaaagga gggagagaag gaagcctggg gcccagccga
     2641 ggaagcgaaa aaccaaacaa gcagttccca ttgtggaacc ccaagaacct gagatcaaac
     2701 taaaatatgc cacccagcca ctggataaaa ctgatgccaa gaacaagtct ttttaccctt
     2761 acatccatgt agtaaataag tgtgaacttg gagccgtttg tacaatcatc aatgctgagg
     2821 aagaagaaca gaccaaatta gtgaggggca ggaagggtca gaggtcactg acccctccac
     2881 ctagcagcac tgaaagcaag gcgctcccgg cctcgtcctt tatgctgcag ggacctgttg
     2941 tgacagagtc ttcggttatg gggcacctgg tttgctgtct gtgtggcaag tgggccagtt
     3001 accggaacat gggtgacctc tttggacctt tttatcccca agattatgca gccactctcc
     3061 cgaagaatcc acctcctaag agggccacag aaatgcagag caaagttaag gtacggcaca
     3121 aaagtgcttc taatggctcc aagacggaca ctgaggagga ggaagagcag cagcagcagc
     3181 agaaggagca gagaagcctg gccgcacacc ccaggtttaa gcggcgccac cgctcggaag
     3241 actgtggtgg aggccctcgg tccctgtcca gggggctccc ttgtaaaaaa gcagccactg
     3301 agggcagcag tgaaaagact gttttggact cgaagccctc cgtgcccacc acttcagaag
     3361 gtggccctga gctggagtta caaatccctg aactacctct tgacagcaat gaattttggg
     3421 tccatgaggg ttgtattctc tgggccaatg gaatctacct ggtttgtggc aggctctatg
     3481 gcctgcagga agcgctggaa atagccagag agatgaaatg ttcccactgc caggaggcag
     3541 gcgccacctt gggctgctac aacaaaggct gctccttccg ataccattac ccgtgtgcca
     3601 ttgatgcaga ttgtttgcta catgaggaga acttctcggt gaggtgccct aagcacaagc
     3661 ctccccttcc gtgccctctc ccccccttgc agaacaagac cgcgaaaggc agcctcagca
     3721 cagagcagtc ggagcggggg tgtacagact acaaagacca tgacggtgat tataaagatc
     3781 atgacatcga ctacaaggat gacgatgaca agtaagaatt ctgatgactc gagataatca
     3841 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     3901 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     3961 tttcattttc tcctccttgt ataaatcctg gttagttctt gccacggcgg aactcatcgc
     4021 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     4081 gtttatttgt gaaatttgtg atgctattgc tttatttgta accatctagc tttatttgtg
     4141 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     4201 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa
     4261 gatctggaga ccggtgtggc acgtgcggac cgagcggccg caggaacccc tagtgatgga
     4321 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc
     4381 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca
     4441 ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     4501 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4561 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4621 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4681 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
     4741 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4801 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc
     4861 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     4921 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
     4981 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     5041 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     5101 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     5161 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     5221 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5281 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5341 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5401 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5461 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5521 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5581 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     5641 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5701 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5761 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5821 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5881 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     5941 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6001 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6061 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6121 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6181 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6241 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6301 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6361 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6421 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6481 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     6541 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6601 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6661 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6721 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6781 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6841 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6901 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6961 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7021 tggccttttg ctcacatgt
//
