LOCUS       .                       7229 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1493..3942
                     /product="sodium channel protein type 8 subunit alpha
                     isoform 1"
                     /translation="CVQRFKCCQVNIEEGLGKSWWILRKTCFLIVEHNWFETFIIFMIL
                     LSSGALAFEDIYIEQRKTIRTILEYADKVFTYIFILEMLLKWTAYGFVKFFTNAWCWLD
                     FLIVAVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSI
                     MNVLLVCLIFWLIFSIMGVNLFAGKYHYCFNETSEIRFEIEDVNNKTECEKLMEGNNTE
                     IRWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRKPDEQPKYEDNIYMYIYFV
                     IFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPI
                     PRPLNKIQGIVFDFVTQQAFDIVIMMLICLNMVTMMVETDTQSKQMENILYWINLVFVI
                     FFTCECVLKMFALRHYYFTIGWNIFDFVVVILSIVGMFLADIIEKYFVSPTLFRVIRLA
                     RIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIFSIFGMSNFAYVKHEAG
                     IDDMFNFETFGNSMICLFQITTSAGWDGLLLPILNRPPDCSLDKEHPGSGFKGDCGNPS
                     VGIFFFVSYIIISFLIVVNMYIAIILENFSVATEESADPLSEDDFETFYEIWEKFDPDA
                     TQFIEYCKLADFADALEHPLRVPKPNTIELIAMDLPMVSGDRIHCLDILFAFTKRVLGD
                     SGELDILRQQMEERFVASNPSKVSYEPITTTLRRKQEEVSAVVLQRAYRGHLARRGFIC
                     KKTTSNKLENGGTHREKKESTPSTASLPSYDSVTKPEKEKQQRAEEGRRERAKRQKEVR
                     ESKC"
                     /gene="SCN8A"
                     /transl_table=1
                     /note="isoform 1 is encoded by transcript variant 1; sodium
                     channel protein type 8 subunit alpha; hNa6/Scn8a
                     voltage-gated sodium channel; voltage-gated sodium channel
                     subunit alpha Nav1.6; sodium channel, voltage gated, type
                     VIII, alpha subunit; voltage-gated sodium channel type VIII
                     alpha protein"
                     /note="color: #993366"
                     /protein_id="NP_055006.1"
                     /db_xref="{'GI': '7657544', 'CCDS': 'CCDS44891.1',
                     'GeneID': '6334', 'HGNC': 'HGNC:10596', 'MIM': '600702'}"
                     /codon_start=1
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /label="SCN8A"
     CDS             5549..6409
                     /product="β-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /transl_table=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /codon_start=1
                     /label="AmpR"
     rep_origin      6580..7168
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     rep_origin      4707..5162
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     terminator      4018..4449
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   4492..4632
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     promoter        5444..5548
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     CDS             3949..4014
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /transl_table=1
                     /codon_start=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     CDS             288..1318
                     /product="site-specific recombinase"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /transl_table=1
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /protein_id=""
                     /codon_start=1
                     /label="Cre"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     protein_bind    1367..1400
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /label="lox2272-JTZ17"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggttgtgtc
     1501 cagcggttca agtgctgcca ggtcaacatc gaggaagggc taggcaagtc ttggtggatc
     1561 ctgcggaaaa cctgcttcct catcgtggag cacaactggt ttgagacctt catcatcttc
     1621 atgattctgc tgagcagtgg cgccctggcc ttcgaggaca tctacattga gcagagaaag
     1681 accatccgca ccatcctgga atatgctgac aaagtcttca cctatatctt catcctggag
     1741 atgttgctca agtggacagc ctatggcttc gtcaagttct tcaccaatgc ctggtgttgg
     1801 ctggacttcc tcattgtggc tgtctcttta gtcagcctta tagctaatgc cctgggctac
     1861 tcggaactag gtgccataaa gtcccttagg accctaagag ctttgagacc cttaagagcc
     1921 ttatcacgat ttgaagggat gagggtggtg gtgaatgcct tggtgggcgc catcccctcc
     1981 atcatgaatg tgctgctggt gtgtctcatc ttctggctga ttttcagcat catgggagtt
     2041 aacttgtttg cgggaaagta ccactactgc tttaatgaga cttctgaaat ccgatttgaa
     2101 attgaagatg tcaacaataa aactgaatgt gaaaagctta tggaggggaa caatacagag
     2161 atcagatgga agaacgtgaa gatcaacttt gacaatgttg gggcaggata cctggccctt
     2221 cttcaagtag caaccttcaa aggctggatg gacatcatgt atgcagctgt agattcccgg
     2281 aagcctgatg agcagcctaa gtatgaggac aatatctaca tgtacatcta ttttgtcatc
     2341 ttcatcatct tcggctcctt cttcaccctg aacctgttca ttggtgtcat cattgataac
     2401 ttcaatcaac aaaagaaaaa gttcggaggt caggacatct tcatgaccga agaacagaag
     2461 aagtactaca atgccatgaa aaagctgggc tcaaagaagc cacagaaacc tattccccgc
     2521 cccttgaaca aaatccaagg aatcgtcttt gattttgtca ctcagcaagc ctttgacatt
     2581 gttatcatga tgctcatctg ccttaacatg gtgacaatga tggtggagac agacactcaa
     2641 agcaagcaga tggagaacat cctctactgg attaacctgg tgtttgttat cttcttcacc
     2701 tgtgagtgtg tgctcaaaat gtttgcgttg aggcactact acttcaccat tggctggaac
     2761 atcttcgact tcgtggtagt catcctctcc attgtgggaa tgttcctggc agatataatt
     2821 gagaaatact ttgtttcccc aaccctattc cgagtcatcc gattggcccg tattgggcgc
     2881 atcttgcgtc tgatcaaagg cgccaaaggg attcgtaccc tgctctttgc cttaatgatg
     2941 tccttgcctg ccctgttcaa catcggcctt ctgctcttcc tggtcatgtt catcttctcc
     3001 atttttggga tgtccaattt tgcatatgtg aagcacgagg ctggtatcga tgacatgttc
     3061 aactttgaga catttggcaa cagcatgatc tgcctgtttc aaatcacaac ctcagctggt
     3121 tgggatggcc tgctgctgcc catcctaaac cgcccccctg actgcagcct agataaggaa
     3181 cacccaggga gtggctttaa gggagattgt gggaacccct cagtgggcat cttcttcttt
     3241 gtaagctaca tcatcatctc tttcctaatt gtcgtgaaca tgtacattgc catcatcctg
     3301 gagaacttca gtgtagccac agaggaaagt gcagaccctc tgagtgagga tgactttgag
     3361 accttctatg agatctggga gaagttcgac cccgatgcca cccagttcat tgagtactgt
     3421 aagctggcag actttgcaga tgccttggag catcctctcc gagtgcccaa gcccaatacc
     3481 attgagctca tcgctatgga tctgccaatg gtgagcgggg atcgcatcca ctgcttggac
     3541 atcctttttg ccttcaccaa gcgggtcctg ggagatagcg gggagttgga catcctgcgg
     3601 cagcagatgg aagagcggtt cgtggcatcc aatccttcca aagtgtctta cgagccaatc
     3661 acaaccacac tgcgtcgcaa gcaggaggag gtatctgcag tggtcctgca gcgtgcctac
     3721 cggggacatt tggcaaggcg gggcttcatc tgcaaaaaga caacttctaa taagctggag
     3781 aatggaggca cacaccggga gaaaaaagag agcaccccat ctacagcctc cctcccgtcc
     3841 tatgacagtg taactaaacc tgaaaaggag aaacagcagc gggcagagga aggaagaagg
     3901 gaaagagcca aaagacaaaa agaggtcaga gaatccaagt gttgtacaga ctacaaagac
     3961 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtaactc
     4021 gagataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     4081 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4141 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttagttctt gccacggcgg
     4201 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     4261 attccgtggt gtttatttgt gaaatttgtg atgctattgc tttatttgta accatctagc
     4321 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     4381 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag
     4441 gttttttaaa gatctggaga ccggtgtggc acgtgcggac cgagcggccg caggaacccc
     4501 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     4561 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     4621 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     4681 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     4741 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     4801 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4861 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4921 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4981 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5041 tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5101 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     5161 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     5221 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     5281 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     5341 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     5401 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     5461 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5521 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     5581 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     5641 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     5701 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     5761 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     5821 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     5881 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5941 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6001 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6061 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6121 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     6181 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     6241 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     6301 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6361 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6421 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6481 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6541 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6601 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6661 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6721 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     6781 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     6841 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6901 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6961 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7021 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7081 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7141 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7201 ggccttttgc tggccttttg ctcacatgt
//
